Abstract：
:An experiment was designed to investigate the reaction mechanism of AP (apurinic or apyrimidinic) DNA endonucleases (APcI, APcII, APcIII) purified from rat liver chromatin. Sulfhydryl compounds (2-mercaptoethanol, dithiothreitol) brought about optimal activities of AP DNA endonucleases and N-ethylmaleimide or HgCl2 inhibited the enzyme activities, indicating the presence of sulfhydryl group at or near the active sites of the enzymes. Mg2+ was essential and 4mM of Mg2+ was sufficient for the optimal activities of AP DNA endonucleases. Km values of APcI, APcII and APcIII for the substrate (E. coli chromosomal AP DNA) were 0.53, 0.27 and 0.36 microM AP sites, respectively. AMP was the most potent inhibitor among adenine nucleotides tested and the inhibition was uncompetitive with respective to the substrate. The Ki values of APcI, APcII and APcIII were 0.35, 0.54 and 0.41mM, respectively. The degree of nick translation of AP DNAs nicked by APcI, APcII and APcIII with Klenow fragment in the presence and absence of T4 polynucleotide kinase or alkaline phosphatase were the same, suggesting that all 3 AP DNA endonucleases excise the phosphodiester bond of AP DNA strand to release 3-hydroxyl nucleotides and 5-phosphomonoester nucleotides.
journal_name
J Korean Med Scijournal_title
Journal of Korean medical scienceauthors
Kim YS,Kim JW,Lee SE,Oh SHdoi
10.3346/jkms.1990.5.3.137subject
Has Abstractpub_date
1990-09-01 00:00:00pages
137-43issue
3eissn
1011-8934issn
1598-6357journal_volume
5pub_type
杂志文章abstract：:Cardiovascular mortality is associated with vascular calcification (VC) in hemodialysis (HD) patients. The present study was designed to find factors related with medial artery calcification on the plain radiography of feet by comparing C-reactive protein (CRP), plasminogen activator inhibitor type 1 (PAI-1) and lipid...
journal_title：Journal of Korean medical science
pub_type： 杂志文章
doi：10.3346/jkms.2009.24.S1.S115
更新日期：2009-01-01 00:00:00
abstract：:The objective of this study was to evaluate the prevalence of asthma, allergic rhinitis, and atopic dermatitis, as well as the risk factors of wheezing among young adults in the Korean military. Young military conscripts in five areas completed a modified International Study of Asthma and Allergies in Childhood (ISAAC...
journal_title：Journal of Korean medical science
pub_type： 杂志文章
doi：10.3346/jkms.2011.26.2.201
更新日期：2011-02-01 00:00:00
abstract：:Central obesity has been reported as a risk for atherosclerosis and metabolic syndrome. The influence of central obesity on diurnal blood pressure (BP) has not been established. In this study, we investigated the influence of central obesity on the circadian parameters of BP by 24 hr ambulatory BP monitoring. Total 1,...
journal_title：Journal of Korean medical science
pub_type： 杂志文章
doi：10.3346/jkms.2013.28.10.1461
更新日期：2013-10-01 00:00:00
abstract：BACKGROUND:The objective of this study was to examine changes in the prevalence of cytotoxic-associated gene A (CagA) positive Helicobacter pylori infection in Jinju, Korea, over the last 20 years. METHODS:Three cross-sectional analyses were conducted concurrently. A total of 1,305 serum samples were collected from 19...
journal_title：Journal of Korean medical science
pub_type： 杂志文章
doi：10.3346/jkms.2020.35.e259
更新日期：2020-08-17 00:00:00
abstract：:We examined the possible anti-inflammatory mechanisms of gabapentin in the attenuation of neuropathic pain and the interaction between the anti-allodynic effects of gabapentin and interleukin-10 (IL-10) expression in a rat model of neuropathic pain. The anti-allodynic effect of intrathecal gabapentin was examined over...
journal_title：Journal of Korean medical science
pub_type： 杂志文章
doi：10.3346/jkms.2013.28.2.308
更新日期：2013-02-01 00:00:00
abstract：:Lack of surface Fas expression is a main route for apoptotic resistance which is considered an important mechanism of tumorigenesis and tumor progression. Fas and FasL expressions in 110 non-small cell lung carcinomas (NSCLCs) were investigated to evaluate their roles in pulmonary carcinogenesis and to examine the cli...
journal_title：Journal of Korean medical science
pub_type： 杂志文章
doi：10.3346/jkms.2005.20.5.770
更新日期：2005-10-01 00:00:00
abstract：BACKGROUND:Mechanism and predictive biomarkers for tyrosine kinase inhibitor (TKI) resistance of advanced clear cell renal cell carcinoma (ccRCC) have not been fully evaluated. METHODS:We performed gene expression profiling on samples from an acquired TKI resistance cohort that consisted of 10 cases of TKI-treated ccR...
journal_title：Journal of Korean medical science
pub_type： 杂志文章
doi：10.3346/jkms.2020.35.e31
更新日期：2020-02-10 00:00:00
abstract：:As of April 18, 2020, there have been a total of 10,653 confirmed cases and 232 deaths due to coronavirus disease 2019 (COVID-19) in Korea. The pathogen spread quickly, and the outbreak caused nationwide anxiety and shock. This study presented the anecdotal records that provided a detailed process of the multidiscipli...
journal_title：Journal of Korean medical science
pub_type： 杂志文章,评审
doi：10.3346/jkms.2020.35.e211
更新日期：2020-06-08 00:00:00
abstract：:The aim of this study was to determine whether maternal serum C-reactive protein (CRP) is of value in predicting funisitis and early-onset neonatal sepsis (EONS) in women with preterm labor or preterm premature rupture of membranes (PROM). This retrospective cohort study included 306 consecutive women with preterm lab...
journal_title：Journal of Korean medical science
pub_type： 杂志文章
doi：10.3346/jkms.2012.27.6.674
更新日期：2012-06-01 00:00:00
abstract：:This study aimed to investigate pressure changes of renal pelvis and histological change of kidneys in a surgically induced sterile rabbit vesicoureteral reflux (VUR) model. Five rabbits served as a control group, 7 as the sham-operated group, and 8 served as the VUR group. Three weeks later, urodynamic studies were p...
journal_title：Journal of Korean medical science
pub_type： 杂志文章
doi：10.3346/jkms.2010.25.9.1352
更新日期：2010-09-01 00:00:00
abstract：BACKGROUND:Although hypertension (HTN) is a well-established major risk factor for renal progression in patients with chronic kidney disease (CKD), few studies investigating its role in renal deterioration in the general population with normal renal function (NRF) have been published. Here, we analyzed the correlation ...
journal_title：Journal of Korean medical science
pub_type： 杂志文章
doi：10.3346/jkms.2020.35.e312
更新日期：2020-08-31 00:00:00
abstract：:Tumor-to-tumor metastasis is rare. We report a case of metastatic renal cell carcinoma in meningioma. A 67-year-old woman presented a two-week history of motor dysphagia and decreased short-term memory. She had undergone a left radical nephrectomy for a renal cell carcinoma 7 years ago, and had not received any adjuva...
journal_title：Journal of Korean medical science
pub_type： 杂志文章
doi：10.3346/jkms.2000.15.5.593
更新日期：2000-10-01 00:00:00
abstract：:In order to evaluate the role of visceral and subcutaneous fat tissue in insulin sensitivity and lipid metabolism, we measured the fasting levels of plasma free fatty acid (FFA) and insulin, glucose disappearance rate (Rd), and hepatic glucose production rate (HGP) after surgical removal of visceral (VF) or subcutaneo...
journal_title：Journal of Korean medical science
pub_type： 杂志文章
doi：10.3346/jkms.1999.14.5.539
更新日期：1999-10-01 00:00:00
abstract：:Development of a cystic mass on the nasal dorsum is a very rare complication of aesthetic rhinoplasty. Most reported cases are of mucous cyst and entrapment of the nasal mucosa in the subcutaneous space due to traumatic surgical technique has been suggested as a presumptive pathogenesis. Here, we report a case of dors...
journal_title：Journal of Korean medical science
pub_type： 杂志文章
doi：10.3346/jkms.2008.23.6.1109
更新日期：2008-12-01 00:00:00
abstract：:The GSTP1 and NQO1 have been reported to be associated with an increased risk for smoking related head and neck squamous cell carcinoma (HNSCC). The purpose of this study was to determine the effect of these metabolic gene polymorphisms on the risk of HNSCC. The study population included 294 histologically confirmed H...
journal_title：Journal of Korean medical science
pub_type： 杂志文章
doi：10.3346/jkms.2006.21.6.1075
更新日期：2006-12-01 00:00:00
abstract：:Interleukin (IL)-33 is an important mediator of innate immunity. Behcet's disease (BD) is an autoinflammatory disorder characterized by hyperactivity of the innate immune response. We measured serum levels of IL-33 and its receptor soluble ST2 (sST2) in patients with BD to investigate their association with disease ac...
journal_title：Journal of Korean medical science
pub_type： 杂志文章
doi：10.3346/jkms.2013.28.8.1145
更新日期：2013-08-01 00:00:00
abstract：:Terminal or interstitial deletions of Xp (Xp22.2→Xpter) in males have been recognized as a cause of contiguous gene syndromes showing variable association of apparently unrelated clinical manifestations such as Leri-Weill dyschondrosteosis (SHOX), chondrodysplasia punctata (CDPX1), mental retardation (NLGN4), ichthyos...
journal_title：Journal of Korean medical science
pub_type： 杂志文章
doi：10.3346/jkms.2012.27.10.1273
更新日期：2012-10-01 00:00:00
abstract：BACKGROUND:We purposed to evaluate the seasonality and associated factors of the incidence of gout attacks in Korea. METHODS:We prospectively enrolled patients with gout attacks who were treated at nine rheumatology clinics between January 2015 and July 2018 and followed them for 1-year. Demographic data, clinical and...
journal_title：Journal of Korean medical science
pub_type： 杂志文章,多中心研究
doi：10.3346/jkms.2020.35.e133
更新日期：2020-05-25 00:00:00
abstract：:In the present study, the authors investigated the management of mechanical valve thrombosis (MVT). From January 1981 through March 2006, 2,908 mechanical valve replacements were performed in 2,298 patients at our institution. Twenty (0.87%) patients presented with MVT, 14 (70.0%) were women, and the mean age of the p...
journal_title：Journal of Korean medical science
pub_type： 杂志文章
doi：10.3346/jkms.2008.23.3.378
更新日期：2008-06-01 00:00:00
abstract：:Sarcoidosis is a systemic granulomatous disease of unknown etiology that involves many organs, occasionally mimicking malignancy. We herein report a 50-yr-old woman of muscular sarcoidosis of chronic myopathic type, manifested by hypercalcemia and muscle wasting. Besides insignificant hilar lymphadenopathy, her sarcoi...
journal_title：Journal of Korean medical science
pub_type： 杂志文章
doi：10.3346/jkms.2013.28.9.1399
更新日期：2013-09-01 00:00:00
abstract：Background:The aim of this study was to evaluate the use of the Diagnostic Drawing Series (DDS) as a screening tool for the breast cancer patients with psychological distress. Methods:All of 64 patients with breast cancer participated in this study. Patients' depressive and anxiety symptoms were assessed using Patient...
journal_title：Journal of Korean medical science
pub_type： 杂志文章
doi：10.3346/jkms.2018.33.e140
更新日期：2018-04-19 00:00:00
abstract：:c-erbB-2 oncogene encodes a growth factor receptor whose amino acid sequence has extensive homology with human epidermal growth factor receptor. It is frequently overexpressed in human breast, ovary, lung, and stomach cancers, where its overexpression is related significantly to the prognosis. Tl investigate the possi...
journal_title：Journal of Korean medical science
pub_type： 杂志文章
doi：10.3346/jkms.1993.8.2.153
更新日期：1993-04-01 00:00:00
abstract：:Conjoined twins show varying degree of conjoining in either facing or side-by-side fashion. Cephalothoracopagus janiceps is a prototype of facing anomaly in which the two bodies demonstrated a cross symmetry to the midline, that is axial symmetry. Interfacial and intersternal lines crossed at a right angle and no abno...
journal_title：Journal of Korean medical science
pub_type： 杂志文章
doi：10.3346/jkms.1988.3.1.19
更新日期：1988-03-01 00:00:00
abstract：:A case of Cushing's syndrome due to bilateral pigmented nodular adrenal disease in a 35-year-old male is presented. The adrenals showed multiple, black, variable sized nodules. Histologically the cells contained lipofuscin and either had a clear cytoplasm or an eosinophilic cytoplasm with a prominent nucleus. Lymphocy...
journal_title：Journal of Korean medical science
pub_type： 杂志文章
doi：10.3346/jkms.1994.9.6.450
更新日期：1994-12-01 00:00:00
abstract：:Most of malignant tumors arising in ovarian and extraovarian endometriosis are carcinomas. Mixed mullerian tumor and endometrial stromal sarcoma arising in intestinal endometriosis are rarely described, but its clinicopathologic features have not been well characterized. Here we report a case of endometrial stromal sa...
journal_title：Journal of Korean medical science
pub_type： 杂志文章,评审
doi：10.3346/jkms.2002.17.3.412
更新日期：2002-06-01 00:00:00
abstract：:The aim of this study was to assess the effectiveness of active intervention with antenatal maternal corticosteroid and antibiotics therapy in infants delivered between 24 and 28 weeks of gestation after premature rupture of membrane. This retrospective study included pregnant women complicated by preterm delivery at ...
journal_title：Journal of Korean medical science
pub_type： 杂志文章
doi：10.3346/jkms.2005.20.1.88
更新日期：2005-02-01 00:00:00
abstract：:We aimed to know the risk-stratification-based prevalence of bacterial contamination of ambulance vehicle surfaces, equipment, and materials. This study was performed in a metropolitan area with fire-based single-tiered Basic Life Support ambulances. Total 13 out of 117 ambulances (11.1%) were sampled and 33 sites per...
journal_title：Journal of Korean medical science
pub_type： 杂志文章
doi：10.3346/jkms.2011.26.1.124
更新日期：2011-01-01 00:00:00
abstract：BACKGROUND:Endoscopic diagnosis of atrophic gastritis can contribute to risk stratification and thereby tailored screening for gastric cancer. We aimed to evaluate the effect of training on inter-observer agreement in diagnosis and grading of endoscopic atrophic gastritis (EAG) according to the level of endoscopists' e...
journal_title：Journal of Korean medical science
pub_type： 杂志文章
doi：10.3346/jkms.2018.33.e117
更新日期：2018-04-09 00:00:00
abstract：:This study was done to characterize the natural course of C-peptide levels in patients with type 1 diabetes and identify distinguishing characters among patients with lower rates of C-peptide decline. A sample of 95 children with type 1 diabetes was analyzed to retrospectively track serum levels of C-peptide, HbA1c, w...
journal_title：Journal of Korean medical science
pub_type： 杂志文章
doi：10.3346/jkms.2013.28.9.1340
更新日期：2013-09-01 00:00:00
abstract：BACKGROUND:The left internal thoracic artery (LITA) has been used as the first conduit of choice in coronary artery bypass grafting (CABG) because of excellent long-term patency and outcomes. However, no studies have examined substances other than nitric oxide that could be beneficial for the bypass conduit, native cor...
journal_title：Journal of Korean medical science
pub_type： 杂志文章
doi：10.3346/jkms.2019.34.e104
更新日期：2019-04-08 00:00:00
