Correlation of somatic cell steroid secretion and quality of generated oocytes after in-vitro stimulation of mouse follicles.

Abstract:

PURPOSE:To test the possibility of follicular somatic cell steroidogenesis as a marker for quality of their embraced oocytes. METHODS:Mechanically isolated mouse preantral follicles were cultured and matured in-vitro (IVC/IVM) for study. RESULTS:During IVC/IVM, oogenesis occurred concomitantly with folliculogenesis in a coordinated manner and simultaneously with progressive increments of somatic cell steroidogenesis. Follicular E(2) production of matured oocytes were significantly higher than that of immature ones. The majority of MII oocytes (32/36) and all developed blastocysts(12/12) were associated with active E(2) production prior to ovulation. In this study, 18 MII oocytes met both requirements for active and optimal E(2) production. 13 of them were fertilized and 10 developed into blastocysts. CONCLUSION:Active somatic cell steroidogenesis prior to ovulation and an optimal steroid milieu at ovulation are prerequisites for generation of competent oocytes after follicular maturation in-vitro.

journal_name

J Assist Reprod Genet

authors

Liu HC,He Z,Rosenwaks Z

doi

10.1007/s10815-006-9041-5

subject

Has Abstract

pub_date

2006-04-01 00:00:00

pages

191-8

issue

4

eissn

1058-0468

issn

1573-7330

journal_volume

23

pub_type

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