Abstract：
:Multidrug resistance transporters (MDRs) are excellent candidates for molecular-level biomonitoring - they function in exporting xenobiotic compounds and their expression is inducible. However, currently available MDR sequence information from aquatic invertebrates is partial and mostly biased towards the conserved ATPase domain. In the present study, two genes belonging to the MDR/TAP (ABCB) family were cloned and characterized from the bivalve Brachidontes pharaonis, which thrives in rocky environments along the Israeli Mediterranean coast. One of these is a complete sequence of a 'half'ABCB, probably belonging to the ABCB10 subfamily, while the second is a 'full'ABCB1 transporter. A quantitative RT-PCR protocol for biomonitoring was tested in laboratory experiments. Bivalves exposed to diesel showed significant increase in B1 expression levels, while the expression of B10 was suppressed. These results suggest that B. pharaonis features an MDR1 homologue that is induced by pollution and may serve as a sentinel organism for routine biomonitoring programs. However, our findings also exemplify that not all MDRs are equally suitable for this purpose and sequence information must be expanded beyond the ATPase domain for correct classification of cloned genes.
journal_name
Mar Environ Resjournal_title
Marine environmental researchauthors
Feldstein T,Nelson N,Mokady Odoi
10.1016/j.marenvres.2006.04.007subject
Has Abstractpub_date
2006-07-01 00:00:00pages
S118-21eissn
0141-1136issn
1879-0291pii
S0141-1136(06)00052-3journal_volume
62 Supplpub_type
杂志文章abstract：:During the last decades, marine pollution with anthropogenic litter has become a worldwide major environmental concern. Standardized monitoring of litter since 2001 on 78 beaches selected within the framework of the Convention for the Protection of the Marine Environment of the North-East Atlantic (OSPAR) has been use...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2013.08.013
更新日期：2013-12-01 00:00:00
abstract：:Competition for space is a remarkable ecological force, comparable to predation, producing a strong selective pressure on benthic invertebrates. Some invertebrates, thus, possess antimicrobial compounds to reduce surface bacterial growth. Antimicrobial inhibition is the first step in avoiding being overgrown by other ...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2015.02.001
更新日期：2015-04-01 00:00:00
abstract：:Irregular sea urchins such as the spatangoid Spatangus purpureus are important bioturbators that contribute to natural biogenic disturbance and the functioning of biogeochemical cycles in soft sediments. In the coastal waters of the Balearic Islands S. purpureus occurs in soft red algal beds, and can reach high densit...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2011.01.008
更新日期：2011-05-01 00:00:00
abstract：:Intersexuality has been found in both males and females of the marine/estuarine amphipod, Echinogammarus marinus, at polluted and reference sites in East Scotland. Polluted sites had significantly more intersex specimens than reference sites, however the cause of intersexuality is unclear. Discriminant analysis of mor...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2004.03.013
更新日期：2004-08-01 00:00:00
abstract：:Shrimps of the Palaemon genus have been affected for over 15 years by a problem of exoskeletal deformities, particularly in the Gironde estuary (France). Given the large numbers of individuals affected, this study focus on a better description of the phenomenon to estimate its impact on shrimps. This study, on Palaemo...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2009.09.001
更新日期：2010-04-01 00:00:00
abstract：:Adopting an integrated approach the potential genotoxic, cytotoxic and developmental effects of tributyltin oxide (TBTO), a known endocrine-disrupting agent for neogastropods, have been evaluated in two ecologically relevant invertebrates: Mytilus edulis (blue mussel) and Platynereis dumerilli (rag worm). Following de...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/s0141-1136(00)00112-4
更新日期：2000-07-01 00:00:00
abstract：:Silver nanoparticles (Ag-NPs) are increasingly used in a wide range of consumer products and such an extensive use raises questions about their safety and environmental toxicity. We investigated the potential toxicity of Ag-NPs in the marine ecosystem by analyzing the effects on several organisms belonging to differen...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2015.06.001
更新日期：2015-10-01 00:00:00
abstract：:Environmental changes have been associated with natural climatic variability or human activity. Water resources management is, perhaps, the most drastic change observed in the coastal environment. However, external forcings such as the El Niño event have important implications in the global and regional hydrological b...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2020.105135
更新日期：2020-10-01 00:00:00
abstract：:This study examined total mercury (Hg) concentrations in cartilaginous fishes from Southern New England coastal waters, including smooth dogfish (Mustelus canis), spiny dogfish (Squalus acanthias), little skate (Leucoraja erinacea), and winter skate (Leucoraja ocellata). Total Hg in dogfish and skates were positively ...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2014.05.009
更新日期：2014-08-01 00:00:00
abstract：:Reef-building species play key roles in promoting local species richness and regulating ecosystem functions like biogeochemical fluxes. We evaluated the functioning of a habitat engineered by the reef-building polychaete Sabellaria alveolata, by measuring oxygen and nutrient fluxes in the reef structures and in the so...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2020.105092
更新日期：2020-12-01 00:00:00
abstract：:The Portuguese continental coast is influenced by several oceanographic processes and is located near the confluence of three biogeographic realms (from the North Atlantic, South Atlantic and Mediterranean). Given these features, the topography of the Portuguese coast, possible variations in fishing effort and reporte...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2020.105044
更新日期：2020-09-01 00:00:00
abstract：:Accurate and reliable benthic quality indicators are in great demand following the recent developments and the strict time schedule for implementing the European Water Framework Directive. The Mondego estuary has experienced a progressive deterioration during the 1990s, followed by a partial ecological recovery due to...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2009.04.001
更新日期：2009-07-01 00:00:00
abstract：:Anthropogenic emissions of carbon dioxide are leading to decreases in pH and changes in the carbonate chemistry of seawater. Ocean acidification may negatively affect the ability of marine organisms to produce calcareous structures while also influencing their physiological responses and growth. The aim of this study ...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2014.04.009
更新日期：2014-08-01 00:00:00
abstract：:Benzothiazole, a common chemical associated with tire manufacturing and industrial wastewater, is a principal component of both fresh water and estuarine tire leachate, a neurotoxicant to larval sheepshead minnows (Cyprinodon variegatus) in in vivo estuarine studies. The neurotoxic potential of benzothiazole was inves...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/s0141-1136(00)00090-8
更新日期：2000-07-01 00:00:00
abstract：:Several genes normally induced by estradiol (E(2)) in female fish, those for vitellogenins (VTGs) and zona radiata proteins (ZRPs), are also inducible in males exposed to estrogenic chemicals. Male sheepshead minnows (SHM) were exposed to both E(2) and para-nonylphenol (NP), at several doses and times to determine a d...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2004.03.043
更新日期：2004-08-01 00:00:00
abstract：:Halogenated aromatic hydrocarbons (HAHs) and polynuclear aromatic hydrocarbons (PAHs) are major environmental contaminants. Fish species that are chronically exposed to these compounds can develop resistance to their toxic effects. In all fish species studied to date, toxicant resistance has been accompanied by decrea...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/s0141-1136(02)00184-8
更新日期：2002-09-01 00:00:00
abstract：:Gadolinium (Gd), a metal of the lanthanide series used as contrast agent for magnetic resonance imaging, is released into the aquatic environment. We investigated the effects of Gd on the development of four sea urchin species: two from Europe, Paracentrotus lividus and Arbacia lixula, and two from Australia, Heliocid...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2016.06.001
更新日期：2017-07-01 00:00:00
abstract：:As part of an effort to validate the use of heat shock proteins (hsp) as a biomarker of adverse effect, we investigated the impact of estrogen (E2), the hormone responsible for vitellogenisis in fish, on the induction of hsp 60 and 70. PLHC-1 (Pociliopsis lucida hepatoma cell line) cells were cultured under standard c...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/s0141-1136(00)00130-6
更新日期：2000-07-01 00:00:00
abstract：:The purpose of this paper is to describe the status and general faunal composition of sponge reefs in the Georgia Basin (GB), British Columbia, Canada. Fourteen distinct deep-water glass sponge (Hexactinellid) reefs have been mapped using multibeam bathymetry and sidescan sonar in the GB. Seven of these have been surv...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2008.09.002
更新日期：2008-12-01 00:00:00
abstract：:Laeonereis acuta was seasonally collected in an industrially polluted site (P) and in an unpolluted site (UP) at the Patos Lagoon estuary (southern Brazil). Glutathione S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT) activity (U/mg protein) was determined in five groups of worms from each site. Meta...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2004.03.053
更新日期：2004-08-01 00:00:00
abstract：:The sulfur metabolite dimethylsulfoniopropionate (DMSP) is the most important precursor of the climate relevant metabolite dimethylsulfide (DMS). It has thus gained interest in the context of climate change and several studies investigated the influence of elevated temperature and/or CO(2) on DMSP in complex plankton ...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2011.11.002
更新日期：2012-02-01 00:00:00
abstract：:Mummichogs, Fundulus heteroclitus, an estuarine fish with a relatively small home range found along the eastern coast of the United States are well-suited to monitoring contaminant effects, including those of polycyclic aromatic hydrocarbons (PAHs). One of the common PAHs in estuaries is pyrene. We report here on effo...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2003.11.001
更新日期：2004-06-01 00:00:00
abstract：:Concentrations of Zn, Pb, Cu and Cd have been determined in leaves, stems and roots of Spartina maritima and Halimione portulacoides from the Tagus estuary salt mash (Corroios) and in the sediments between their roots. Biological materials and sediments were sampled every 2 months, between July 1991 and July 1992. Roo...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/s0141-1136(99)00077-x
更新日期：2000-04-01 00:00:00
abstract：:Sulphidic mine waste disposed in marine environments constitutes an environmental risk to aquatic life due to potential uptake and accumulation of heavy metals in biota. Fiord sediments near the former Black Angel Mine in West Greenland are contaminated by lead and zinc as a result of submarine tailings disposal in 19...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/s0141-1136(02)00219-2
更新日期：2003-04-01 00:00:00
abstract：:The new record of a shallow-water submarine hydrothermal field (<150 m w.d.) in the western Mediterranean Sea (Tyrrhenian Sea, Italy) allows us to study CO2 fluid impact on benthic foraminifers. Benthic foraminifers calcification process is sensitive to ocean acidification and to local chemical and physical parameters...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2018.02.015
更新日期：2018-05-01 00:00:00
abstract：:The indiscriminate use of plastic has greatly increased microplastic contamination risk in the marine environment. Microplastics can affect all marine life via the food web, from primary producers (e.g., microalgae) to final consumers (e.g., carnivorous fish). Thus, several studies have attempted to evaluate microplas...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2019.104838
更新日期：2019-11-09 00:00:00
abstract：:Oysters Crassostrea rhizophorae and Crassostrea gigas were kept for fourteen days at four sites in São José, SC, Brazil, chosen according to a sewage discharge contamination gradient. Enzymatic activities of CAT, GST, G6PDH and GR were evaluated in gills and digestive glands. Higher CAT activity was observed in tissue...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2008.02.057
更新日期：2008-07-01 00:00:00
abstract：:The need to develop powerful tools to detect exposure and effects of POPs and emerging contaminants in Mediterranean cetaceans led us to develop a suite of sensitive non-lethal biomarkers in integument biopsies of free-ranging animals. In order to propose induction of CYP1A1 and CYP2B, detected by Western blot analysi...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2008.02.006
更新日期：2008-07-01 00:00:00
abstract：:Contamination by lead (Pb) and zinc (Zn) was studied in seawater, sediments, seaweeds and blue mussels near the former Black Angel Pb-Zn Mine in Maarmorilik, West Greenland. The mine operated during the period 1973-90 when mine waste (tailings and later waste rock) was discharged directly into the sea. Metal concentra...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2011.03.001
更新日期：2011-06-01 00:00:00
abstract：:Estuarine environmental assessment by sub-individual responses is important in order to understand contaminant effects and to find suitable estuarine biomonitor species. Our study aimed to analyze oxidative stress responses, including glutathione-S-transferase (GST) activity, total antioxidant capacity (ACAP) and lipi...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2012.10.012
更新日期：2013-02-01 00:00:00
