Abstract：
OBJECTIVE:Mesenchymal stem cells (MSC) raise high hopes in clinical applications. However, the lack of common standards and a precise definition of MSC preparations remains a major obstacle in research and application of MSC. Whereas surface antigen markers have failed to precisely define this population, a combination of proteomic data and microarray data provides a new dimension for the definition of MSC preparations. METHODS:In our continuing effort to characterize MSC, we have analyzed the differential transcriptome and proteome expression profiles of MSC preparations isolated from human bone marrow under two different expansion media (BM-MSC-M1 and BM-MSC-M2). RESULTS:In proteomics, 136 protein spots were unambiguously identified by MALDI-TOF-MS and corresponding cDNA spots were selected on our "Human Transcriptome cDNA Microarray." Combination of datasets revealed a correlation in differential gene expression and protein expression of BM-MSC-M1 vs BM-MSC-M2. Genes involved in metabolism were more highly expressed in BM-MSC-M1, whereas genes involved in development, morphogenesis, extracellular matrix, and differentiation were more highly expressed in BM-MSC-M2. Interchanging culture conditions for 8 days revealed that differential expression was retained in several genes whereas it was altered in others. CONCLUSION:Our results have provided evidence that homogeneous BM-MSC preparations can reproducibly be isolated under standardized conditions, whereas culture conditions exert a prominent impact on transcriptome, proteome, and cellular organization of BM-MSC.
journal_name
Exp Hematoljournal_title
Experimental hematologyauthors
Wagner W,Feldmann RE Jr,Seckinger A,Maurer MH,Wein F,Blake J,Krause U,Kalenka A,Bürgers HF,Saffrich R,Wuchter P,Kuschinsky W,Ho ADdoi
10.1016/j.exphem.2006.01.002keywords:
subject
Has Abstractpub_date
2006-04-01 00:00:00pages
536-48issue
4eissn
0301-472Xissn
1873-2399pii
S0301-472X(06)00006-3journal_volume
34pub_type
杂志文章abstract：OBJECTIVE:The mechanisms of mobilization of hematopoietic stem cells (HSC) from bone marrow to peripheral blood (PB) by cytokines are poorly understood. One hypothesis is that cytokines disrupt cytoadhesive interactions of stem cells with bone marrow stroma. The soluble portion of c-kit (s-kit) binds stem cell factor (...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/j.exphem.2004.01.004
更新日期：2004-04-01 00:00:00
abstract：:A thymic stromal cell line with a medullary phenotype (Z210R.1) supported the differentiation of surface IgM+ B cells when cocultured with fetal liver cells in vitro. Conditioned medium (CM) from this cell line supported the long-term growth of a B cell line (NAG8/7) isolated from cocultures and enhanced the prolifera...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1994-03-01 00:00:00
abstract：:The development of pluripotential hematopoietic stem cells (PHSC) requires the continuous support provided by the bone tissue and bone marrow (BM) stromal cells. The basic rule of spatial and temporal organization of the distinct stromal cells and differentiating hematopoietic cells in the course of development, regen...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1995-04-01 00:00:00
abstract：OBJECTIVE:To investigate the role of perforin-mediated cell apoptosis in murine models of immune-mediated bone marrow (BM) failure. MATERIALS AND METHODS:We compared C57BL/6J (B6) mice carrying a perforin gene deletion (Prf(-/-)) with wild-type (WT) controls for cellular composition in lymphohematopoietic tissues. Lym...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/j.exphem.2008.12.001
更新日期：2009-04-01 00:00:00
abstract：:We established a clonal cell culture system for human natural killer (NK) cells from fetal thymocytes. Thymocytes of 16 to 22 gestational weeks were cultured in methylcellulose in the presence of interleukin (IL)-7, IL-15, and steel factor (SF). After 14 days in incubation, large, diffuse colonies consisting of small ...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/s0301-472x(99)00005-3
更新日期：1999-04-01 00:00:00
abstract：:Hematopoietic stem cell transplantation (HSCT) is an established treatment for multiple myeloma (MM), a plasma cell malignancy. To identify an improved pretransplant conditioning regimen, we investigated the cytotoxicity of gemcitabine (Gem) and clofarabine (Clo) combinations toward MM cell lines and patient cell samp...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/j.exphem.2013.04.009
更新日期：2013-08-01 00:00:00
abstract：OBJECTIVE:To design a culture method allowing the quantitative and qualitative analysis of terminal erythroid differentiation. METHODS:Primary erythroid progenitors derived either from mouse tissues or from human umbilical cord blood were differentiated using hanging drop cultures and compared to methylcellulose cultu...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/j.exphem.2005.06.014
更新日期：2005-10-01 00:00:00
abstract：:To investigate the value of genetic mutations in the pathogenesis and differential diagnosis of hemophagocytic lymphohistiocytosis (HLH), mutations related to blood and immune deficiency genes were analyzed in patients with HLH. Peripheral blood samples from 33 children diagnosed with HLH on the basis of the 2004 diag...
journal_title：Experimental hematology
pub_type： 临床试验,杂志文章
doi：10.1016/j.exphem.2019.09.024
更新日期：2019-10-01 00:00:00
abstract：:Bone marrow cell responses to therapeutic doses of cis-diamminedichloroplatinum (II) (Cis-Pt) were evaluated in female C57B1/6 mice by determining bone marrow cellularity and content of transplantable colony forming units (CFUS) after treatment. Although limitation on the dosage of Cis-Pt is generally considered to be...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1981-03-01 00:00:00
abstract：OBJECTIVE:The primary objective of this study was to identify and clone the first nonmammalian thrombopoietin (TPO), chicken TPO, and its receptor c-Mpl for the purpose of characterizing their activities both in vitro and in vivo. MATERIALS AND METHODS:Chicken TPO was cloned using the methods of reverse transcriptase ...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/j.exphem.2007.12.001
更新日期：2008-04-01 00:00:00
abstract：:Although three molecular forms of macrophage colony-stimulating factor (M-CSF) have been reported, Western blot analysis of immunoaffinity-purified M-CSF from human blood has shown that the major species of M-CSF in the serum has a molecular weight (MW) of 85 kD. Superose-12 gel filtration chromatography of immunoaffi...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1996-02-01 00:00:00
abstract：:Recent experimental data suggest that the heterogeneity of chronic myeloid leukemia (CML) stem cells may be the result of the development of unique molecular events generating functional consequences in terms of the resistance and persistence of leukemic stem cells. To explore this phenomenon, we designed a single-cel...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/j.exphem.2020.04.005
更新日期：2020-05-01 00:00:00
abstract：:Disruption of ribosomal proteins is associated with hematopoietic phenotypes in cell culture and animal models. Mutations in ribosomal proteins are seen in patients with Diamond Blackfan anemia, a rare congenital disease characterized by red cell aplasia and distinctive craniofacial anomalies. A zebrafish screen uncov...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/j.exphem.2011.11.007
更新日期：2012-03-01 00:00:00
abstract：:Tumor necrosis factor alpha (TNFalpha) is thought to contribute to the blunted erythropoietin (Epo) production in inflammatory diseases. The present study was carried out to find out as to whether the 55 kD (TNF-RI) or the 75 kD (TNF-RII) receptor is responsible for the TNFalpha-induced inhibition of hepatic Epo synth...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/s0301-472x(98)00054-x
更新日期：1999-02-01 00:00:00
abstract：:In a previous report, we described a method to generate cell lines derived from stromal colonies (colony-derived cell lines [CDCL]). In a first step, colonies were obtained from plating cells from adherent layers of human long-term bone marrow cultures (LTBMC) in methyl-cellulose in the presence of interleukin-1 beta ...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1995-02-01 00:00:00
abstract：:Interferon (INF)-α was the maintenance treatment of choice after autologous stem cell transplantation in multiple myeloma in the past, but currently Thalidomide is commonly used. In this prospective study, the implications of the various types of maintenance therapy on the patients T cell pattern and activation status...
journal_title：Experimental hematology
pub_type： 临床试验,杂志文章
doi：10.1016/j.exphem.2012.10.018
更新日期：2013-03-01 00:00:00
abstract：:The in vitro and in vivo effects of murine recombinant interferon-gamma (rIFN-gamma) on hematopoietic and immune parameters of normal mice and of mice bearing metastatic variant Lewis lung carcinoma (LLC-C3) tumors were assessed. The in vitro addition of rIFN-gamma to bone marrow or spleen cells from normal and LLC-C3...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1988-05-01 00:00:00
abstract：:The course of development of graft-versus-host reactions (GVHR) following lethal irradiation was compared in two mouse donor-host combinations disparate at non H-2 loci alone or associated to H-2 complex. The pathogenesis of these reactions was very different (mortality rates faster in the second situation for example...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1981-05-01 00:00:00
abstract：OBJECTIVES:To identify the regulatory sequences driving Gata1 expression in conventional dendritic cells (cDC). MATERIALS AND METHODS:The number and expression levels of Gata1, Gata1-target genes and hypersensitive site (HS) 2 (the eosinophil-specific enhancer)-driven green fluorescent protein (GFP) reporter of cDCs f...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/j.exphem.2010.03.006
更新日期：2010-06-01 00:00:00
abstract：:The effects of a single injection of hemin on murine marrow BFU-E and CFU-S were assessed to determine whether hemin is as effective in augmenting primitive (day 7) BFU-E levels in situ as it is in vitro and to assess hemin's action on transplantable pluripotent stem cells (CFU-S). The results show that hemin exerts a...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1984-11-01 00:00:00
abstract：:With a new labeling technique in radiation chimeras, an attempt was made to determine the duration of the phases of the stem cell cycle including shortest and mean generation time and to estimate the number of hemopoietic stem cells per unit of bone marrow cellularity. The DNA of bone marrow cells in DNA synthesis was...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1979-10-01 00:00:00
abstract：:We have investigated the effect of hematopoietic cytokines on the survival of severe combined immune-deficient (SCID) mice that received a high dose of radiation. In this study, female SCID mice were irradiated at doses ranging from 500 to 600 cGy and then transplanted with 2 x 10(6) male Balb/c marrow cells. Groups o...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1994-02-01 00:00:00
abstract：:This study evaluated the usefulness of a matrix culture system, the artificial capillary culture system, for the growth of hematopoietic cells, the maintenance of CFUS and the collection of GM-CSF. The system was compared to monolayer, gelfoam sponge and Dexter liquid cultures. Monolayer cultures of adherent lymphohem...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1981-02-01 00:00:00
abstract：:Based on previous observations that granulocyte-macrophage colony-stimulating factor (GM-CSF) promotes granulocyte recovery following chemotherapy, we evaluated the effect of recombinant human GM-CSF on hematopoietic progenitors and clinical outcome in six patients with delayed engraftment (greater than 55 days) after...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1991-03-01 00:00:00
abstract：:Two main aspects of the in vitro mouse foetal liver cell assay for Erythroid Stimulating Factor (ESF) in human sera have been investigated. The haem extraction process has been shown to give specific and quantitative recovery of 59Fe labelled haem from haemoglobin thus confirming that the material assayed in human ser...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1975-11-01 00:00:00
abstract：OBJECTIVE:Progenipoietin-1 is an agonist of both the granulocyte colony-stimulating factor and fetal liver tyrosine kinase-3 receptors capable of inducing the proliferation of multiple hematopoietic cell lineages. The potential of progenipoietin-1 to mobilize transplantable hematopoietic stem cells into the peripheral ...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/s0301-472x(01)00675-0
更新日期：2001-08-01 00:00:00
abstract：:The efficacy of binding of several lectins to different Burkitt's lymphoma (BL) cell lines was investigated. Soybean agglutinin (SBA), peanut agglutinin (PNA), wheat germ agglutinin (WGA), and Sambucus nigra agglutinin (SNA) bound strongly to all BL cell lines. Because SBA has been used safely in clinical bone marrow ...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：OBJECTIVE:Several compounds, including butyrate and trichostatin A, have been shown to activate gamma-gene expression via p38 mitogen-activated protein kinase (MAPK) signaling. In eukaryotic cells, reactive oxygen species (ROS) act as signaling molecules to mediate phosphorylation of tyrosine kinases such as p38 MAPK t...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/j.exphem.2005.12.009
更新日期：2006-03-01 00:00:00
abstract：:We have established an in vitro system with which to examine the ability of Abelson murine leukemia virus (A-MuLV) to infect early hemopoietic progenitor cells. Blast cell colonies containing less than 100 cells were shown to contain up to 85% of cells with secondary hemopoietic colony-forming ability. Infection of ce...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1988-01-01 00:00:00
abstract：:Quantitative measurements on the haemopoiedic systems of SAS/4, 3H and RFM mice have been used to calculate the distribution and population sizes of haemopoietic cells in the bone marrow, spleen and peripheral blood. Considerable strain differences have been found in the sizes of haemopioetic stem cell populations and...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1975-06-01 00:00:00
