Abstract:
AIM:Passive immunotherapy using antibody against hepatitis B surface antigen (HBsAg) has been advocated in certain cases of Hepatitis B infection. We had earlier reported on the cloning and expression of a high affinity scFv derived from a mouse monoclonal (5S) against HBsAg. However this mouse antibody cannot be used for therapeutic purposes as it may elicit anti-mouse immune responses. Chimerization by replacing mouse constant domains with human ones can reduce the immunogenicity of this antibody. METHODS:We cloned the V(H) and V(L) genes of this mouse antibody, and fused them with CH1 domain of human IgG1 and C(L) domain of human kappa chain respectively. These chimeric genes were cloned into a phagemid vector. After initial screening using the phage display system, the chimeric Fab was expressed in soluble form in E. coli. RESULTS:The chimeric Fab was purified from the bacterial periplasmic extract. We characterized the chimeric Fab using several in vitro techniques and it was observed that the chimeric molecule retained the high affinity and specificity of the original mouse monoclonal. This chimeric antibody fragment was further expressed in different strains of E. coli to increase the yield. CONCLUSION:We have generated a mouse-human chimeric Fab against HBsAg without any significant loss in binding and epitope specificity. This chimeric Fab fragment can be further modified to generate a full-length chimeric antibody for therapeutic uses.
journal_name
World J Gastroenteroljournal_title
World journal of gastroenterologyauthors
Bose B,Khanna N,Acharya SK,Sinha Sdoi
10.3748/wjg.v11.i48.7569keywords:
subject
Has Abstractpub_date
2005-12-28 00:00:00pages
7569-78issue
48eissn
1007-9327issn
2219-2840journal_volume
11pub_type
杂志文章abstract:AIM:To investigate the effect of Danzhijiangtang capsule (DJC) on monocyte chemoattractant protein-1 (MCP-1) mRNA expression in newly diagnosed type 2 diabetes mellitus (T2DM) subclinical vascular lesions. METHODS:Sixty-two patients with newly diagnosed T2DM subclinical vascular lesions were randomly divided into a co...
journal_title:World journal of gastroenterology
pub_type: 杂志文章,随机对照试验
doi:10.3748/wjg.v19.i19.2963
更新日期:2013-05-21 00:00:00
abstract:AIM:To characterise the viral kinetics of enterovirus 71 (EV71). METHODS:In this study, human rhabdomyosarcoma (RD) cells were infected with EV71 at different multiplicity of infection (MOI). After infection, the cytopathic effect (CPE) was monitored and recorded using a phase contrast microscope associated with a CCD...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v17.i36.4135
更新日期:2011-09-28 00:00:00
abstract:AIM:To understand the role of toll-like receptor 4 (TLR4) signaling in the regulation of iron-regulatory hormone, hepcidin by chronic alcohol consumption. METHODS:For chronic alcohol intake studies, TLR4 mutant mice on C3H/HeJ background and wildtype counterpart on C3H/HeOuJ background were pair-fed with regular (cont...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v20.i34.12161
更新日期:2014-09-14 00:00:00
abstract::Heterotopic pancreatic tissue within the stomach is rare and dysplasia within heterotopic pancreatic tissue is very rare. We present the first report of a patient with concurrent occurrence of heterotopic pancreas in the stomach with a gastrointestinal stromal tumour. ...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v13.i43.5781
更新日期:2007-11-21 00:00:00
abstract:AIM:To investigate the effects of gastric pacing on gastric emptying and plasma motilin level in a canine model of gastric motility disorders and the correlation between gastric emptying and plasma motilin level. METHODS:Ten healthy Mongrel dogs were divided into: experimental group of six dogs and control group of fo...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v10.i3.419
更新日期:2004-02-01 00:00:00
abstract:AIM:To detect distribution and relative frequency of endocrine cells in gastrointestinal tract of flower fish (Pseudophoxinus antalyae). METHODS:The intestinal tract of flower fish was divided into four portions from proximal to distal; the enlarged area after oesophagus and anterior, middle and posterior intestine. I...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v12.i42.6874
更新日期:2006-11-14 00:00:00
abstract:BACKGROUND:Neoadjuvant chemotherapy is currently recommended as preoperative treatment for locally advanced rectal cancer (LARC); however, evaluation of treatment response to neoadjuvant chemotherapy is still challenging. AIM:To create a multi-modal radiomics model to assess therapeutic response after neoadjuvant chem...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v26.i19.2388
更新日期:2020-05-21 00:00:00
abstract:AIM:To establish a model for prognosis assessment of extranodal follicular dendritic cell (FDC) sarcoma. METHODS:Nine lesions were examined by routine and molecular approaches. Clinicopathological factors from the new cases and 97 reported cases were analyzed for their prognostic values. RESULTS:The current lesions w...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v16.i20.2504
更新日期:2010-05-28 00:00:00
abstract:AIM:To investigate the anti-tumor mechanism of antisense oligodeoxynucleotide cantide against hTERT. METHODS:Tumor cells were cultured overnight and grown to 50-60 % confluence. HepG2 and SMMC-7721 were treated with cantide mixed with lipofectin, or lipofectin alone. After inducted for 6 h at 37 degrees, 10 % FCS in D...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v9.i9.2030
更新日期:2003-09-01 00:00:00
abstract::Multiple studies have demonstrated alterations in the intestinal microbial community (termed the microbiome) in Crohn's disease (CD) and several lines of evidence suggest these changes may have a significant role in disease pathogenesis. In active and quiescent disease, both the faecal and mucosa-associated microbiome...
journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v20.i27.8751
更新日期:2014-07-21 00:00:00
abstract:BACKGROUND:Pancreatic cancer is a major cause of cancer-related death, with a 5-year overall survival rate being below 5%. The main causes of poor prognosis in pancreatic cancer include easy metastasis, high recurrence rate, and robust drug resistance. Gemcitabine is a first-line drug for patients with unresectable pan...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v25.i37.5590
更新日期:2019-10-07 00:00:00
abstract:AIM:To investigate the effect of antiviral therapy with nucleoside analogs in hepatitis B virus (HBV)-related cirrhosis and esophageal varices. METHODS:Eligible patients with HBV-related cirrhosis and esophageal varices who consulted two tertiary hospitals in Beijing, China, the Chinese Second Artillery General Hospit...
journal_title:World journal of gastroenterology
pub_type: 杂志文章,多中心研究
doi:10.3748/wjg.v19.i40.6849
更新日期:2013-10-28 00:00:00
abstract::Despite the recent advances in the therapeutic modalities, colorectal cancer (CRC) remains to be one of the most common causes of cancer-related death. CRC arises through accumulation of multiple genetic and epigenetic alterations that transform normal colonic epithelium into adenocarcinomas. Among crucial roles of ep...
journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v20.i4.978
更新日期:2014-01-28 00:00:00
abstract::Gastrointestinal duplication is an uncommon congenital abnormality in two-thirds of cases manifesting before the age of 2 years. Ileal duplication is common while colonic duplication, either cystic or tubular, is a rather unusual clinical entity that remains asymptomatic and undiagnosed in most cases. Mostly occurring...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v11.i32.5072
更新日期:2005-08-28 00:00:00
abstract:AIM:Quasispecies of hepatitis C virus (HCV) are the foundation for rapid sequence evolution of HCV to evade immune surveillance of hosts. The consensus sequence evolution of a segment of HCV NS3 region, which encompasses putative cytotoxic T cell epitopes, was evaluated. METHODS:Three male patients, infected with HCV ...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v10.i6.847
更新日期:2004-03-15 00:00:00
abstract:AIM:To determine the pattern and distribution of colonic diverticulosis in Thai adults. METHODS:A review of the computerized radiology database for double contrast barium enema (DCBE) in Thai adults was performed at the Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand. Incomplete studies and...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v19.i46.8709
更新日期:2013-12-14 00:00:00
abstract:AIM:To show outcomes of our series of patients that underwent a total gastrectomy with a robotic approach and highlight the technical details of a proposed solution for the reconstruction phase. METHODS:Data of gastrectomies performed from May 2014 to October 2016, were extracted and analyzed. Basic characteristics of...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v23.i23.4293
更新日期:2017-06-21 00:00:00
abstract::It is common practice to follow patients with colorectal cancer for some years after resection and/or adjuvant treatment. Data are lacking about how often patients should be seen, what tests should be performed, and what surveillance strategy has a significant impact on patient outcome. Seven randomized trials have ad...
journal_title:World journal of gastroenterology
pub_type: 社论
doi:10.3748/wjg.v16.i12.1427
更新日期:2010-03-28 00:00:00
abstract::Colorectal cancer metastasizes predictably, with liver predominance in most cases. Because liver involvement has been shown to be a major determinant of survival in this population, liver-directed therapies are increasingly considered even in cases where there is (limited) extrahepatic disease. Unfortunately, these pa...
journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v22.i11.3127
更新日期:2016-03-21 00:00:00
abstract:AIM:To determine whether the gastrin stimulated intestinal crypt cell (IEC-6) proliferation by induction of ornithine decarboxylase (ODC). METHODS:IEC-6 cells were grown in DMEM containing 50 mL x L(-1) dialyzed fetal bovine serum for 24h and then were treated with gastrin. The proliferative capability of the cells wa...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v8.i1.183
更新日期:2002-02-01 00:00:00
abstract:AIM:To investigate serum mean platelet volume (MPV) levels in acute pancreatitis (AP) patients and assess whether MPV effectively predicts the disease severity of AP. METHODS:We included 117 consecutive patients with AP as the AP group and 34 consecutive patients with colorectal polyps (before endoscopic treatment) as...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v23.i13.2404
更新日期:2017-04-07 00:00:00
abstract::Cyclophosphamide is a potent cytotoxic agent used in many clinical settings. The main risks of cyclophosphamide therapy include hematological disorders, infertility, hemorrhagic cystitis and malignancies. Gastrointestinal side effects reported to date are often non-specific and not severe. We present the first case of...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v22.i39.8844
更新日期:2016-10-21 00:00:00
abstract:AIM:To evaluate whether the cytokine responses in liver and serum differ in chronic hepatitis C patients with normal and high alanine aminotransferase (ALT) levels. METHODS:Thirty-three (16 with normal ALT level as group 1 and 17 with elevated ALT level as group 2) patients infected with genotype 1b hepatitis C virus ...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v11.i45.7188
更新日期:2005-12-07 00:00:00
abstract:AIM:To study the relationship between the expression of the c-erbB-2 proto-oncogene product with gastric mucosal carcinogenesis and the behavior of gastric carcinoma. METHODS:Specimens from nine normal gastric mucosa, 23 gastric mucosal dysplasia (10 slight, six moderate, seven severe), 18 early gastric carcinoma, and...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v3.i2.122
更新日期:1997-06-15 00:00:00
abstract:AIM:To compare outcome of stapled hemorrhoidopexy (SH) vs LigaSure hemorrhoidectomy (LH) by a meta-analysis of available randomized controlled trials (RCTs). METHODS:Databases, including PubMed, EMBASE, the Cochrane Library, and the Science Citation Index updated to December 2012, were searched. The main outcomes meas...
journal_title:World journal of gastroenterology
pub_type: 杂志文章,meta分析,评审
doi:10.3748/wjg.v19.i29.4799
更新日期:2013-08-07 00:00:00
abstract::Gastroduodenal disease (GDD) was initially thought to be uncommon in Africa. Amongst others, lack of access to optimal health infrastructure and suspicion of conventional medicine resulted in the reported prevalence of GDD being significantly lower than that in other areas of the world. Following the increasing availa...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v25.i26.3344
更新日期:2019-07-14 00:00:00
abstract::Gluten-sensitive enteropathy, also known as coeliac disease (CD), is an autoimmune disorder occurring in genetically susceptible individuals that damages the small intestine and interferes with the absorption of other nutrients. As it is triggered by dietary gluten and related prolamins present in wheat, rye and barle...
journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v20.i21.6495
更新日期:2014-06-07 00:00:00
abstract:AIM:To evaluate the pharmaceutical safety of a Chinese herbal formula, chunggan extract (CGX), traditionally prescribed as a hepatotherapeutic drug via systemic acute and subacute toxicological study. METHODS:Twenty male dogs and 20 female dogs were fed doses 50 times and 4 times greater than the clinically-recommende...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v12.i46.7497
更新日期:2006-12-14 00:00:00
abstract:AIM:To investigate the role of neoadjuvant chemoradiotherapy in prognosis and surgery for esophageal carcinoma by a meta-analysis. METHODS:PubMed and manual searches were done to identify all published randomized controlled trials (RCTs) that compared neoadjuvant chemoradiotherapy plus surgery (CRTS) with surgery alon...
journal_title:World journal of gastroenterology
pub_type: 杂志文章,meta分析,评审
doi:10.3748/wjg.15.4962
更新日期:2009-10-21 00:00:00
abstract::A case of strangulation of the transverse colon in a traumatic left diaphragmatic hernia manifesting as pericarditis is reported. This is unusual because pericardial signs in traumatic diaphragmatic hernia have been previously described in association with direct pericardial injury. This is the only such case where el...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v13.i9.1449
更新日期:2007-03-07 00:00:00