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DNA damage detection by an archaeal single-stranded DNA-binding protein.

Abstract：

:Archaeal DNA repair pathways are not well defined; in particular, there are no convincing candidate proteins for detection of DNA mismatches or the bulky lesions removed by excision repair pathways. Single-stranded DNA-binding proteins (SSBs) play a central role in DNA replication, recombination and repair. The crenarchaeal SSB is a monomer with a single oligonucleotide-binding fold for single-stranded DNA binding coupled to a flexible C-terminal tail reminiscent of bacterial SSB that mediates interactions with other proteins. We demonstrate that Sulfolobus solfataricus SSB can melt DNA containing a mismatch or DNA lesion specifically in vitro. We suggest that a potential role for SSB in archaea is the detection of DNA damage due to local destabilisation of the DNA double helix, followed by recruitment of specific repair proteins. Proteins interacting specifically with a single-stranded DNA:SSB complex include several known or putative DNA repair proteins and DNA helicases.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Cubeddu L,White MF

doi

10.1016/j.jmb.2005.08.050

keywords:

subject

Has Abstract

pub_date

2005-10-28 00:00:00

pages

507-16

issue

3

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(05)01004-1

journal_volume

353

pub_type

杂志文章

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