Abstract:
:A new type of solute importer has been identified recently in various bacterial genera and called the tripartite tricarboxylate transporter (TTT). TTTs consist of two cytoplasmic membrane proteins and a periplasmic solute-binding protein. In the whooping cough agent Bordetella pertussis, a TTT system that has been called BctCBA mediates the uptake of citrate, with BctA and BctB being the membrane components and BctC, the periplasmic protein. Here, we describe that the expression of the bctCBA operon is induced by the presence of citrate in the milieu. The signalling cascade involves both BctC and the signal transduction two-component system BctDE, encoded by an operon adjacent to bctCBA. Furthermore, two-hybrid analyses and affinity chromatography experiments indicated that citrate-liganded BctC interacts with the periplasmic domain of the sensor protein, BctE. Thus, BctC is part of the signalling cascade leading to upregulation of the transporter operon in the presence of its solute, a new function for periplasmic binding proteins of TT transporters.
journal_name
J Mol Bioljournal_title
Journal of molecular biologyauthors
Antoine R,Huvent I,Chemlal K,Deray I,Raze D,Locht C,Jacob-Dubuisson Fdoi
10.1016/j.jmb.2005.05.071keywords:
subject
Has Abstractpub_date
2005-08-26 00:00:00pages
799-809issue
4eissn
0022-2836issn
1089-8638pii
S0022-2836(05)00653-4journal_volume
351pub_type
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