Abstract:
:The LIM homeobox family of transcription factors is involved in many processes during the development of the mammalian central nerves system. L3, also called Lhx8 (L3/Lhx8), is a recently identified member of the LIM homeobox gene family and is selectively expressed in the medial ganglionic eminence (MGE). Our previous study demonstrated that L3/Lhx8-null mice specifically lacked cholinergic neurons in the basal forebrain. In this study, we reduced L3/Lhx8 function in the murine neuroblastoma cell line, Neuro2a (N2a), using L3/Lhx8-targeted small interfering RNA (siRNA) produced by H1.2 promoter-driven vector. The levels of cholinergic markers per cell were diminished without a reduction in the number of marker-positive cells. Intriguingly, GABAergic marker expression and the number of GABAergic cells were dramatically increased in the differentiating L3/Lhx8-knockdown N2a. These results suggest the possibility that L3/Lhx8 is involved in the determination of transmitter phenotypes (GABAergic or cholinergic cell fate) in a population of neurons during basal forebrain development.
journal_name
J Neurochemjournal_title
Journal of neurochemistryauthors
Manabe T,Tatsumi K,Inoue M,Matsuyoshi H,Makinodan M,Yokoyama S,Wanaka Adoi
10.1111/j.1471-4159.2005.03261.xkeywords:
subject
Has Abstractpub_date
2005-08-01 00:00:00pages
723-30issue
3eissn
0022-3042issn
1471-4159pii
JNC3261journal_volume
94pub_type
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