Abstract:
:Delivery of plasmid DNA for protein production in mammalian cells is not an efficient process. In this study, the theory that cellular localization of plasmid DNA increases transfection efficiency is examined with an emphasis on the understanding of the cellular association of the components of a ternary transfection complex. Mammalian cells take up transfection reagent-DNA complexes primarily from their local environment. Via formation of a ternary complex consisting of the DNA-transfection reagent complex and a heavy particle, such as silica, the efficiency of transfection is substantially increased. We have analyzed cells transfected with the ternary complexes to determine if sedimentation alone affects the percentage of cells that contain the complexes or specific components of the complex. A significant fraction of cells associate with the ternary complexes, including silica nanoparticles. The percentage of cells that associate with DNA was not significantly influenced by the use of the ternary complex. This result suggests that the silica nanoparticles are more than just a sedimentation agent, being also a secondary transfection reagent. These data also confirm that cells may contain transfection reagent-DNA complex but do not express the protein of interest. This knowledge will be used in further research to better design transfection reagents that will increase the efficiency of protein production.
journal_name
Mol Pharmjournal_title
Molecular pharmaceuticsauthors
Guo C,Gemeinhart RAdoi
10.1021/mp049969akeywords:
subject
Has Abstractpub_date
2004-07-01 00:00:00pages
309-16issue
4eissn
1543-8384issn
1543-8392journal_volume
1pub_type
杂志文章abstract::Within the immune system there is an exquisite ability to discriminate between "self" and "non-self" that is orchestrated by antigen-specific T lymphocytes. Genomic plasticity enables differentiation of naive CD4+ T lymphocytes into either regulatory cells (Treg) that express the transcription factor Foxp3 and activel...
journal_title:Molecular pharmaceutics
pub_type: 杂志文章
doi:10.1021/mp100203a
更新日期:2011-02-07 00:00:00
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journal_title:Molecular pharmaceutics
pub_type: 杂志文章
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journal_title:Molecular pharmaceutics
pub_type: 杂志文章
doi:10.1021/mp900024z
更新日期:2009-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2014-01-06 00:00:00
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journal_title:Molecular pharmaceutics
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pub_type: 杂志文章
doi:10.1021/acs.molpharmaceut.0c00177
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更新日期:2012-09-04 00:00:00
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pub_type: 杂志文章
doi:10.1021/mp500464b
更新日期:2015-04-06 00:00:00
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pub_type: 杂志文章
doi:10.1021/acs.molpharmaceut.0c00982
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pub_type: 杂志文章
doi:10.1021/mp300202c
更新日期:2012-09-04 00:00:00
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journal_title:Molecular pharmaceutics
pub_type: 杂志文章
doi:10.1021/mp400531n
更新日期:2013-12-02 00:00:00
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pub_type: 杂志文章
doi:10.1021/mp100425p
更新日期:2011-04-04 00:00:00
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pub_type: 杂志文章
doi:10.1021/mp200621v
更新日期:2012-06-04 00:00:00
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journal_title:Molecular pharmaceutics
pub_type: 杂志文章
doi:10.1021/mp300461b
更新日期:2013-01-07 00:00:00
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journal_title:Molecular pharmaceutics
pub_type: 杂志文章
doi:10.1021/acs.molpharmaceut.8b00588
更新日期:2018-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1021/acs.molpharmaceut.8b01237
更新日期:2019-03-04 00:00:00
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pub_type: 杂志文章
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更新日期:2014-01-06 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2012-03-05 00:00:00
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journal_title:Molecular pharmaceutics
pub_type: 杂志文章
doi:10.1021/acs.molpharmaceut.0c00478
更新日期:2020-10-05 00:00:00
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journal_title:Molecular pharmaceutics
pub_type: 杂志文章
doi:10.1021/acs.molpharmaceut.7b00068
更新日期:2017-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1021/mp400619v
更新日期:2014-03-03 00:00:00
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journal_title:Molecular pharmaceutics
pub_type: 杂志文章
doi:10.1021/acs.molpharmaceut.7b00343
更新日期:2017-08-07 00:00:00
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journal_title:Molecular pharmaceutics
pub_type: 杂志文章
doi:10.1021/acs.molpharmaceut.5b00723
更新日期:2016-03-07 00:00:00
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更新日期:2018-03-05 00:00:00
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journal_title:Molecular pharmaceutics
pub_type: 杂志文章
doi:10.1021/acs.molpharmaceut.8b00672
更新日期:2018-11-05 00:00:00
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journal_title:Molecular pharmaceutics
pub_type: 杂志文章
doi:10.1021/acs.molpharmaceut.6b01146
更新日期:2017-08-07 00:00:00