Abstract：
:The acetylcholinesterase inhibitor, acotiamide, improves gastric motility and is clinically used to treat functional dyspepsia. The present study aimed to identify the transporters involved in the distribution of acotiamide in stomach tissue. Acotiamide uptake by the gastric cancer-derived model cell line, Hs746 T, was Na+- and pH-independent. The initial uptake velocity of acotiamide was saturable with increasing concentrations of acotiamide and was inhibited by selective serotonin reuptake inhibitors, which are potent inhibitors of the plasma membrane monoamine transporter (PMAT). The uptake of acotiamide by PMAT gene-transfected HEK293 cells was saturable, with similar Km (197.9 μM) values to those of uptake by Hs 746T cells (106 μM). Moreover, immunoreactivity of PMAT was found in the gastric smooth muscle and vascular endothelial cells. These results suggest that PMAT contributes to the distribution of acotiamide in the stomach, where it exerts its pharmacological effects.
journal_name
Mol Pharmjournal_title
Molecular pharmaceuticsauthors
Hirayama M,Hoshino Y,Yoshii K,Toda R,Kawabata Y,Nakanishi T,Tamai Idoi
10.1021/acs.molpharmaceut.9b00894subject
Has Abstractpub_date
2020-04-06 00:00:00pages
1071-1078issue
4eissn
1543-8384issn
1543-8392journal_volume
17pub_type
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