Abstract:
:The objective was to determine the disposition of polymer nanoparticles and an associated, lipophilic, model "active" component on and within the skin following topical application. Polystyrene and poly(methyl methacrylate) nanoparticles containing covalently bound fluorescein methacrylate and dispersed Nile Red were prepared by emulsion polymerization. The two fluorophores differentiate the fate of the polymeric vehicle on and within the skin from that of the active. Nanoparticles were characterized by dynamic light scattering, transmission electron microscopy and NMR spectroscopy. In vitro skin permeation experiments were performed using dermatomed porcine skin. Post-treatment with nanoparticle formulations, the skin surface was either cleaned carefully with buffer or simply dried with tissue, and then immediately visualized by confocal microscopy. Average nanoparticle diameters were below 100 nm. Confocal images showed that nanoparticles were located in skin "furrows" and around hair follicles. Surface cleaning removed the former but not all of the latter. At the skin surface, Nile Red remained partly associated with nanoparticles, but was also released to some extent and penetrated into deeper layers of the stratum corneum (SC). In summary, polymeric nanoparticles did not penetrate beyond the superficial SC, showed some affinity for hair follicles, and released an associated "active" into the skin.
journal_name
Mol Pharmjournal_title
Molecular pharmaceuticsauthors
Wu X,Price GJ,Guy RHdoi
10.1021/mp9001188subject
Has Abstractpub_date
2009-09-01 00:00:00pages
1441-8issue
5eissn
1543-8384issn
1543-8392journal_volume
6pub_type
杂志文章abstract::Polycationic polymers like poly(ethylene imine)s (PEIs) are extensively explored for the nonviral transfer of DNA or small RNAs (siRNAs). To enhance biocompatibility and alter pharmacokinetic properties, hyperbranched PEI was recently grafted with the nonligand oligosaccharides maltose or maltotriose at various degree...
journal_title:Molecular pharmaceutics
pub_type: 杂志文章
doi:10.1021/mp400479g
更新日期:2013-12-02 00:00:00
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pub_type: 杂志文章
doi:10.1021/mp500504q
更新日期:2015-04-06 00:00:00
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更新日期:2012-11-05 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章,评审
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更新日期:2014-01-06 00:00:00
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pub_type: 杂志文章
doi:10.1021/mp3006209
更新日期:2013-06-03 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1021/acs.molpharmaceut.8b01013
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pub_type: 杂志文章
doi:10.1021/acs.molpharmaceut.6b01023
更新日期:2017-04-03 00:00:00
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pub_type: 杂志文章
doi:10.1021/acs.molpharmaceut.0c00380
更新日期:2020-07-06 00:00:00
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journal_title:Molecular pharmaceutics
pub_type: 杂志文章
doi:10.1021/acs.molpharmaceut.7b00361
更新日期:2017-09-05 00:00:00
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journal_title:Molecular pharmaceutics
pub_type: 杂志文章
doi:10.1021/acs.molpharmaceut.7b00884
更新日期:2018-03-05 00:00:00
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journal_title:Molecular pharmaceutics
pub_type: 杂志文章
doi:10.1021/mp200315c
更新日期:2012-05-07 00:00:00
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journal_title:Molecular pharmaceutics
pub_type: 杂志文章
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pub_type: 杂志文章,评审
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journal_title:Molecular pharmaceutics
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更新日期:2014-01-06 00:00:00
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pub_type: 杂志文章
doi:10.1021/acs.molpharmaceut.7b00404
更新日期:2017-11-06 00:00:00
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更新日期:2013-01-07 00:00:00
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doi:10.1021/acs.molpharmaceut.5b00089
更新日期:2015-09-08 00:00:00
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更新日期:2019-01-07 00:00:00
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更新日期:2014-03-03 00:00:00
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pub_type: 杂志文章
doi:10.1021/acs.molpharmaceut.5b00582
更新日期:2015-12-07 00:00:00