Abstract:
:The major goal of wound healing biology is to determine how a wound can be induced to repair damaged tissue faster and more efficiently. Enhancement of dermal and epidermal regeneration is an extremely important goal for the treatment of many different types of wounds. Exogenous application of growth factors to the wound site has been shown to have potential to improve wound healing. Frequent applications of large amounts of growth factor have been required. This is because proteases in the wound quickly destroy peptide growth factor. Gene therapy has the potential to produce growth factors deep within the wound, where they can be effective as well as able to constantly replenish growth factor that is destroyed by peptidases. We have shown that application of plasmid DNA expression vectors directly into the wound is an inefficient modality. Electroporation, the application of an electrical field across cells to permeabilize the cell membrane has led us to explore the possibility of utilizing the technique to enhance transfection efficiency. We have identified electroporation parameters that improve the efficiency of DNA transfection in cutaneous wounds, and we have shown that electroporation itself does not impair wound healing. We are now on the threshold of exploring whether electroporation-assisted transfection with DNA plasmid expression vectors for growth factors will be an effective modality for enhancing cutaneous wound healing.
journal_name
World J Surgjournal_title
World journal of surgeryauthors
Ferguson M,Byrnes C,Sun L,Marti G,Bonde P,Duncan M,Harmon JWdoi
10.1007/s00268-004-2062-2keywords:
subject
Has Abstractpub_date
2005-01-01 00:00:00pages
S55-9eissn
0364-2313issn
1432-2323journal_volume
29 Suppl 1pub_type
杂志文章,评审abstract::Angiogenesis is of vital importance during the development and progression of solid tumors. Vascular endothelial growth factor (VEGF) is a major regulator of angiogenesis and could be produced by some cancer cells. To investigate the clinical relevance of VEGF in the tumorigenesis of human thyroid, an immunohistochemi...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s002680020085
更新日期:2001-03-01 00:00:00
abstract:BACKGROUND:Artificial intelligence is touted as the future of medicine. Classical algorithms for the detection of common bile duct stones (CBD) have had poor clinical uptake due to low accuracy. This study explores the challenges of developing and implementing a machine-learning model for the prediction of CBD stones i...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-020-05820-8
更新日期:2021-02-01 00:00:00
abstract:BACKGROUND:Parathyroid cancer is rare. Differentiating parathyroid carcinoma from degenerative changes at histopathology can be difficult and studies investigating the value of single immunohistochemical markers have had variable results. In this study we aimed to investigate whether a panel of immunohistochemistry mar...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-014-2700-2
更新日期:2014-11-01 00:00:00
abstract:BACKGROUND:It is now generally believed that regulatory T cells (Tregs) play an important role in peripheral tolerance, and a defect in Tregs is considered one of the most important factors in the induction of various kinds of autoimmune disease including ulcerative colitis (UC). Here, we examined the change in frequen...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-005-0301-9
更新日期:2006-04-01 00:00:00
abstract:BACKGROUND:Selective nonoperative management (SNOM) of penetrating neck injuries (PNI) has steadily gained favor, but indications for surgery and adjunctive diagnostic studies remain debated. The purpose of the present study is to validate a protocol of SNOM of PNI based on physical examination, which further dictates ...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-008-9766-7
更新日期:2008-12-01 00:00:00
abstract::Recently, surgical services have been gaining greater attention as an integral part of public health in low-income countries due to the significant volume and burden of surgical conditions, growing evidence of the cost-effectiveness of surgical intervention, and global disparities in surgical care. Nonetheless, there ...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-010-0585-2
更新日期:2010-11-01 00:00:00
abstract::Breast cancer is a common disease, and the surgical management is continually evolving. The objective of this study was to describe the current breast cancer practice patterns among Canadian surgeons. All active General Surgeons (n=1172), as accredited by the Royal College of Physicians and Surgeons of Canada, were se...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-003-7040-6
更新日期:2004-01-01 00:00:00
abstract:BACKGROUND:Alternative methods to overcome limitations of electromyogram (EMG) tube applied for intraoperative neuromonitoring (IONM) of recurrent laryngeal nerve (RLN) during thyroidectomy have been introduced. In this study, we evaluated the feasibility of adhesive skin electrodes for IONM of RLN in patients who unde...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-019-05208-3
更新日期:2020-01-01 00:00:00
abstract::Hepatectomy with concomitant resection of the inferior vena cava (IVC) has become common for hepatic malignancies involving the IVC. However, diagnosing IVC invasion and the procedure of choice have yet to be standardized. Medical records of nine patients with liver cancer (five metastatic tumors from colorectal cance...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-003-6908-9
更新日期:2003-06-01 00:00:00
abstract:BACKGROUND:Primary hyperparathyroidism (HPT) is often caused by a benign parathyroid tumor, adenoma; less commonly by multiglandular parathyroid disease/hyperplasia; and rarely by parathyroid carcinoma. Patients with multiple tumors require wider exploration to avoid recurrence and have increased risk for hereditary di...
journal_title:World journal of surgery
pub_type: 杂志文章,评审
doi:10.1007/s00268-009-0022-6
更新日期:2009-11-01 00:00:00
abstract:BACKGROUND:Accurate presurgical assessment is important to anticipate postoperative complications, especially in the growing proportion of elderly cancer patients. We designed a study to define which comorbid conditions at the time of diagnosis predict complications after surgery for colorectal cancer. PATIENTS:A rand...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-005-0711-8
更新日期:2007-01-01 00:00:00
abstract::Mansonic schistosomiasis is an endemic disease in Brazil, with an estimated 10-12 million people infested. Among its clinical manifestations, the hepatosplenic form causes portal hypertension which, in turn, brings about severe digestive hemorrhage, the most serious complication of the disease. Normally, the patients ...
journal_title:World journal of surgery
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/BF01659051
更新日期:1991-03-01 00:00:00
abstract:BACKGROUND:We reported phonatory recovery in the majority of 88 patients after recurrent laryngeal nerve (RLN) reconstruction. Here we analyzed factors that might influence the recovery, in a larger patient series. METHODS:At Kuma Hospital, 449 patients (354 females and 95 males) underwent RLN reconstruction with dire...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-016-3634-7
更新日期:2016-12-01 00:00:00
abstract:BACKGROUND:The goal of the present study was to evaluate the effects of preoperative oral carbohydrate supplementation (OCH) on the postoperative metabolic stress response of patients undergoing elective abdominal surgery. METHODS:The study was designed as a controlled, prospective, cohort study including 38 patients ...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-012-1590-4
更新日期:2012-08-01 00:00:00
abstract::Leiomyomas and leiomyosarcomas are derived from smooth muscle tissues. Smooth muscle cells also surround the blood vessels that supply the alimentary tract. These cells have at times been said to contribute to the formation of smooth muscle tumors in the alimentary canal. With such an abundance of smooth muscle, there...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s002689910063
更新日期:2000-04-01 00:00:00
abstract::To evaluate quality of life and functional results following surgery for gastric cancer we studied 104 patients with no evidence of disease at a minimum of 12 months postoperatively. Patients were treated with total gastrectomy and jejunal pouch reconstruction according to Hunt-Lawrence-Rodino (n = 59) or simple esoph...
journal_title:World journal of surgery
pub_type: 临床试验,杂志文章
doi:10.1007/BF00294722
更新日期:1995-07-01 00:00:00
abstract:INTRODUCTION:The quantification and analysis of adverse events is essential to benchmark surgical outcomes and establish a foundation for quality improvement interventions. We developed a hybrid electronic medical record (HEMR) system for the accurate collection and integration of data into a structured morbidity and m...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-014-2766-x
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:The Severe Acute Respiratory Syndrome Coronavirus 2 pandemic has exposed surgeons to hazardous working conditions, imposing the need for personal protective equipment (PPE) use during surgery. The use of such equipment may affect their non-technical skills, augment fatigue, and affect performance. This study...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-020-05648-2
更新日期:2020-09-01 00:00:00
abstract::This study aims to examine the frequency of the pheochromocytoma (pheo), defined as a "benign, intra-adrenal, hypertensive, sporadic, unilateral tumor." Three large series amounting to 310 subphrenic chromaffin tumors operated over periods of 17, 23, and 41 years, respectively, have been reviewed. Among those combined...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/BF00353738
更新日期:1994-07-01 00:00:00
abstract:BACKGROUND:Uncomplicated acute appendicitis has been managed traditionally by early appendicectomy. However, recently, there has been increasing interest in the potential for primary treatment with antibiotics, with studies finding this to be associated with fewer complications than appendicectomy. The aim of this stud...
journal_title:World journal of surgery
pub_type: 杂志文章,meta分析
doi:10.1007/s00268-016-3561-7
更新日期:2016-10-01 00:00:00
abstract::The present prospective clinical study was carried out to investigate the effect of age on mortality, morbidity, and readmission rates after coronary artery bypass grafting (CABG). Data on 1131 consecutive CABG patients were collected in a surgical center and in all 18 secondary referral hospitals up to 3 months after...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-003-7033-5
更新日期:2003-12-01 00:00:00
abstract:OBJECTIVE:To evaluate comparative outcomes of laparoscopic transcystic (TC) and transductal (TD) common bile duct (CBD) exploration. METHODS:We systematically searched MEDLINE, EMBASE, CINAHL, CENTRAL, the World Health Organization International Clinical Trials Registry, ClinicalTrials.gov, ISRCTN Register, and biblio...
journal_title:World journal of surgery
pub_type: 杂志文章,meta分析
doi:10.1007/s00268-019-05005-y
更新日期:2019-08-01 00:00:00
abstract:BACKGROUND:Patients with hepatocellular carcinoma (HCC) who underwent hepatectomy often developed an intrahepatic recurrence, even though it was a curative one. The relationship between surgery-induced liver damage and the recurrence of HCC has not been described. This study evaluated whether posthepatectomy liver fail...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-013-2247-7
更新日期:2014-01-01 00:00:00
abstract::This retrospective study reports our experience managing 78 patients with adenocarcinoma of the esophagus and gastroesophageal junction operated between January 1982 and December 1996. Altogether 18 patients presented with stage I and II disease, and 60 patients had stage III and IV disease at presentation; 56 patient...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/pl00013179
更新日期:1999-03-01 00:00:00
abstract:BACKGROUND:Chronic anal fissure (CAF) is a linear split of the anoderm. The minimally invasive management of CAF such as botulinum toxin (BT) injection is recommended. However, the exact efficient dose of BT, number of injections per session and the injection sites are still debatable. The aim of this analysis was to a...
journal_title:World journal of surgery
pub_type: 杂志文章,meta分析,评审
doi:10.1007/s00268-016-3693-9
更新日期:2016-12-01 00:00:00
abstract:BACKGROUND:We sought to assess the status of availability of technology for trauma care in a state in India and to identify factors contributing to both adequate levels of availability and to deficiencies. We also sought to identify potential solutions to deficiencies in terms of health system management and product de...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-014-2805-7
更新日期:2015-02-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:We determined the rates of initial lymph node metastasis following curative resection of mass-forming type-intrahepatic cholangiocarcinoma (ICC) in patients with and without hepatitis virus infection. METHODS:We enrolled 87 patients between January 2000 and December 2013 with ICC without preo...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-016-3843-0
更新日期:2017-04-01 00:00:00
abstract::This retrospective study in 79 surgically treated patients with a proximal bile duct carcinoma revealed 12 patients with a median age of 59.5 years (range 21-73 years) who survived more than 5 years. These 12 patients were analyzed to identify specific patient characteristics for long-term survival. Fifteen patients d...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s002689900571
更新日期:1999-01-01 00:00:00
abstract::Surgical oncology is one of the most frequently studied surgical specialties with regard to disparities in quality of care. There is variation in the care received according to nonclinical factors such as age, race and ethnicity, education, income, and even geographic region. Differences exist with regard to who gets ...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-007-9383-x
更新日期:2008-04-01 00:00:00
abstract:BACKGROUND:Medullary thyroid carcinoma (MTC) accounts only for 1.4% of all thyroid malignancies in Japan. Since 1996, we have performed hemithyroidectomy, instead of total thyroidectomy, for sporadic nonhereditary MTC when the primary lesion is located in only one lobe. Regarding lymph node dissection, modified radical...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-008-9795-2
更新日期:2009-01-01 00:00:00