A multigene approach to phylogenetic analysis using the genus Mycobacterium as a model.

Abstract:

:Advances in DNA sequencing and the increasing number of sequences available in databases have greatly enhanced the bacterial identification process. Several species within the genus Mycobacterium cause serious human and animal diseases. In order to assess their relative positions in the evolutionary process, four gene fragments, from the 16S rRNA (564 bp), hsp65 (420 bp), rpoB (396 bp) and sod (408 bp) genes, were sequenced from 97 strains, including all available type strains of the genus Mycobacterium. The results demonstrate that, in this case, the concatenation of different genes allows significant increases in the power of discrimination and the robustness of the phylogenetic tree. The sequential and/or combined use of sequences of several genes makes it possible to refine the phylogenetic approach and provides a molecular basis for accurate species identification.

authors

Devulder G,de Montclos MP,Flandrois JP

doi

10.1099/ijs.0.63222-0

keywords:

subject

Has Abstract

pub_date

2005-01-01 00:00:00

pages

293-302

issue

Pt 1

eissn

1466-5026

issn

1466-5034

journal_volume

55

pub_type

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