Utility of internally transcribed 16S-23S rDNA spacer regions for the definition of Pseudomonas stutzeri genomovars and other Pseudomonas species.

Abstract:

:Bacteria identified and classified as Pseudomonas stutzeri, on the basis of traditional criteria, are recognized to be markedly heterogeneous, such that a systematic phenotypic characterization has not been correlated with genotypic groupings (i.e. genomovars) based upon DNA-DNA similarities. The internally transcribed 16S-23S rDNA spacer (ITS1) regions of P. stutzeri were analysed with respect to the ability of these nucleic acid regions to differentiate and identify the genomic groups (i.e. genomovars) of P. stutzeri. The ITS1s of 34 strains of P. stutzeri were amplified by PCR and the PCR product was subjected to RFLP analysis, which allowed the differentiation and identification of the strains to their respective genomovars. Sequence determination and analysis of ITS1s supported further the results obtained by RFLP, i.e. nucleotide signatures were identified in strains belonging to different genomovars. The ITS1s of all strains of P. stutzeri contained the tandem tRNA(Ile)/tRNA(Ala) genes and did not exhibit distinct sequence heterogeneity between different operons of a strain. Phylogenetically informative variable sites were located, exclusively, in non-coding regions. The results of the RFLP and sequence analysis of ITS1s supported and correlated with the phylogenetic relationships estimated from 16S rRNA gene sequence comparisons and DNA-DNA hybridizations, offering an alternative tool for genomovar and species differentiation.

authors

Guasp C,Moore ER,Lalucat J,Bennasar A

doi

10.1099/00207713-50-4-1629

keywords:

subject

Has Abstract

pub_date

2000-07-01 00:00:00

pages

1629-1639

eissn

1466-5026

issn

1466-5034

journal_volume

50 Pt 4

pub_type

杂志文章
  • Taxonomic relationships among the taxa in the Candida guilliermondii complex, as revealed by comparative electrophoretic karyotyping.

    abstract::Electrophoretic karyotypes of 15 type strains of the taxa in the Candida guilliermondii complex including Candida fukuyamaensis Nakase et al. and Candida xestobii Yarrow et S. A. Meyer were comparatively analysed by using the CHEF (contour-clamped homogeneous electric field) method of PFGE. Eighteen strains (isolated ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/00207713-50-1-417

    authors: Bai FY,Liang HY,Jia JH

    更新日期:2000-01-01 00:00:00

  • Thermaerobacter subterraneus sp. nov., a novel aerobic bacterium from the Great Artesian Basin of Australia, and emendation of the genus Thermaerobacter.

    abstract::A strictly aerobic, thermophilic, gram-positive, spore-producing, rod-shaped bacterium (2.0-10.0 x 0.3 microm), designated isolate C21T, was isolated from a sample collected from an open drain run-off channel of a bore in the Great Artesian Basin of Australia (New Lorne Bore, registered number 17263). Isolate C21T gre...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/00207713-52-3-795

    authors: Spanevello MD,Yamamoto H,Patel BKC

    更新日期:2002-05-01 00:00:00

  • Corynebacterium glaucum sp. nov.

    abstract::A bacterial strain, strain IMMIB R-5091(T), isolated from a cosmetic dye was characterized by phenotypic and molecular taxonomic methods. Chemotaxonomic investigations revealed the presence of cell-wall chemotype IV and short-chain mycolic acids consistent with the genus Corynebacterium. Comparative 16S rRNA gene sequ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.02394-0

    authors: Yassin AF,Kroppenstedt RM,Ludwig W

    更新日期:2003-05-01 00:00:00

  • Blastococcus atacamensis sp. nov., a novel strain adapted to life in the Yungay core region of the Atacama Desert.

    abstract::A polyphasic study was undertaken to establish the taxonomic status of a Blastococcus strain isolated from an extreme hyper-arid Atacama Desert soil. The isolate, strain P6T, was found to have chemotaxonomic and morphological properties consistent with its classification in the genus Blastococcus. It was shown to form...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.002828

    authors: Castro JF,Nouioui I,Sangal V,Choi S,Yang SJ,Kim BY,Trujillo ME,Riesco R,Montero-Calasanz MDC,Rahmani TPD,Bull AT,Sutcliffe IC,Asenjo JA,Andrews B,Goodfellow M

    更新日期:2018-09-01 00:00:00

  • Lactobacillus cypricasei Lawson et al. 2001 is a later heterotypic synonym of Lactobacillus acidipiscis Tanasupawat et al. 2000.

    abstract::The applicability of a multilocus sequence analysis (MLSA)-based identification system for lactobacilli was evaluated. Two housekeeping genes that code for the phenylalanyl-tRNA synthase alpha-subunit (pheS) and RNA polymerase alpha-subunit (rpoA) were sequenced and analysed for members of the Lactobacillus salivarius...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.64229-0

    authors: Naser SM,Vancanneyt M,Hoste B,Snauwaert C,Swings J

    更新日期:2006-07-01 00:00:00

  • Marinitoga okinawensis sp. nov., a novel thermophilic and anaerobic heterotroph isolated from a deep-sea hydrothermal field, Southern Okinawa Trough.

    abstract::A novel thermophilic and sulfur-reducing heterotrophic bacterium, strain TFS10-5(T), was isolated from a deep-sea hydrothermal field in Yonaguni Knoll IV, Southern Okinawa Trough. Cells of strain TFS10-5(T) were motile rods, 1.5-5 microm in length and 0.5-0.8 microm in width. Strain TFS10-5(T) was an obligately anaero...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.64640-0

    authors: Nunoura T,Oida H,Miyazaki M,Suzuki Y,Takai K,Horikoshi K

    更新日期:2007-03-01 00:00:00

  • Knoellia locipacati sp. nov., from soil of the Demilitarized Zone in South Korea.

    abstract::A gram-positive, aerobic, rod- or coccus-shaped, non-motile bacterium, designated DMZ1(T), was isolated from soil of the Demilitarized Zone, South Korea. Strain DMZ1(T) grew optimally at 30 °C, at pH 7-8 and with 1 % (w/v) NaCl. The isolate showed high 16S rRNA gene sequence similarity with Knoellia aerolata 5317S-21(...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.031880-0

    authors: Shin NR,Roh SW,Kim MS,Jung MJ,Whon TW,Bae JW

    更新日期:2012-02-01 00:00:00

  • Cautionary tale of using 16S rRNA gene sequence similarity values in identification of human-associated bacterial species.

    abstract::Modern bacterial taxonomy is based on a polyphasic approach that combines phenotypic and genotypic characteristics, including 16S rRNA sequence similarity. However, the 95 % (for genus) and 98.7 % (for species) sequence similarity thresholds that are currently recommended to classify bacterial isolates were defined by...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.000161

    authors: Rossi-Tamisier M,Benamar S,Raoult D,Fournier PE

    更新日期:2015-06-01 00:00:00

  • Nomenclature and taxonomy of the genus Salmonella.

    abstract::The nomenclature of the genus Salmonella has reached an unsatisfactory state of affairs, with two systems of nomenclature in circulation. One system, proposed in the 1980s by Le Minor and Popoff, has received wide acceptance, although it does not conform to the rules of the Bacteriological Code. The other system, whic...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 评论,杂志文章

    doi:10.1099/ijs.0.63580-0

    authors: Tindall BJ,Grimont PAD,Garrity GM,Euzéby JP

    更新日期:2005-01-01 00:00:00

  • 'Candidatus Phytoplasma luffae', a novel taxon associated with witches' broom disease of loofah, Luffa aegyptica Mill.

    abstract::The phytoplasma associated with witches' broom disease of loofah [Luffa aegyptica Mill., syn. Luffa cylindrica (L.) M.J. Roem.] in Taiwan was classified in group 16SrVIII, subgroup A (16SrVIII-A), based on results from actual and in silico RFLP analysis of 16S rRNA gene sequences. Nucleotide sequencing of PCR-amplifie...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.001980

    authors: Davis RE,Zhao Y,Wei W,Dally EL,Lee IM

    更新日期:2017-08-01 00:00:00

  • Thiobacter subterraneus gen. nov., sp. nov., an obligately chemolithoautotrophic, thermophilic, sulfur-oxidizing bacterium from a subsurface hot aquifer.

    abstract::A novel, thermophilic, obligately chemolithoautotrophic, sulfur/thiosulfate-oxidizing bacterium was isolated from subsurface geothermal aquifer water (temperature approximately 70 degrees C) in the Hishikari gold mine, Japan. Cells of the isolate, designated strain C55T, were motile, straight rods with a single polar ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.63389-0

    authors: Hirayama H,Takai K,Inagaki F,Nealson KH,Horikoshi K

    更新日期:2005-01-01 00:00:00

  • Synonymy of the yeast genera Moniliella and Trichosporonoides and proposal of Moniliella fonsecae sp. nov. and five new species combinations.

    abstract::Analyses of nucleotide sequences from the D1/D2 domains of the large-subunit rDNA and phenotypic characteristics showed that the genera Moniliella and Trichosporonoides are members of a single, monophyletic clade that would be best represented by a single anamorphic genus. On the basis of taxonomic priority, we propos...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.65117-0

    authors: Rosa CA,Jindamorakot S,Limtong S,Nakase T,Lachance MA,Fidalgo-Jiménez A,Daniel HM,Pagnocca FC,Inácio J,Morais PB

    更新日期:2009-02-01 00:00:00

  • Brooklawnia cerclae gen. nov., sp. nov., a propionate-forming bacterium isolated from chlorosolvent-contaminated groundwater.

    abstract::Two novel facultatively anaerobic bacterial strains, BL-34(T) and BL-35, isolated from groundwater contaminated by a mixture of chlorosolvents were characterized using a polyphasic approach. The two strains exhibited essentially identical taxonomic features except for a vitamin B(12) requirement by strain BL-35 for op...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.64317-0

    authors: Bae HS,Moe WM,Yan J,Tiago I,da Costa MS,Rainey FA

    更新日期:2006-08-01 00:00:00

  • Salinibacterium xinjiangense sp. nov., a psychrophilic bacterium isolated from the China No. 1 glacier.

    abstract::A novel psychrophilic, Gram-positive, yellow-pigmented and aerobic bacterium, strain 0543(T), was isolated from the China No. 1 glacier. Strain 0543(T) was able to grow at 4-23 degrees C, with optimum growth at 18-19 degrees C. The major fatty acids were anteiso-C(15 : 0) (58.36 %), iso-C(16 : 0) (21.13 %), iso-C(14 :...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.65802-0

    authors: Zhang DC,Liu HC,Xin YH,Yu Y,Zhou PJ,Zhou YG

    更新日期:2008-12-01 00:00:00

  • Metallosphaera cuprina sp. nov., an acidothermophilic, metal-mobilizing archaeon.

    abstract::A novel acidothermophilic archaeon, strain Ar-4(T), was isolated from a sulfuric hot spring in Tengchong, Yunnan, China. Cells of strain Ar-4(T) were Gram-staining-negative, irregular cocci and motile by means of flagella. Strain Ar-4(T) grew over a temperature range of 55-75 °C (optimum, 65 °C), a pH range of 2.5-5.5...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.026591-0

    authors: Liu LJ,You XY,Guo X,Liu SJ,Jiang CY

    更新日期:2011-10-01 00:00:00

  • Dyadobacter crusticola sp. nov., from biological soil crusts in the Colorado Plateau, USA, and an emended description of the genus Dyadobacter Chelius and Triplett 2000.

    abstract::Bacterial strain CP183-8(T) was isolated from biological soil crusts collected in the Colorado Plateau, USA. Cells of this strain were aerobic, non-motile, Gram-negative, psychrotolerant and formed beaded chains in the stationary growth phase. They contained C(16 : 1)omega5c and C(16 : 1)omega7c as major fatty acids. ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.63498-0

    authors: Reddy GSN,Garcia-Pichel F

    更新日期:2005-05-01 00:00:00

  • Sejongia antarctica gen. nov., sp. nov. and Sejongia jeonii sp. nov., isolated from the Antarctic.

    abstract::Two yellow-pigmented, Gram-negative and aerobic bacterial strains, designated AT1013T and AT1047T, were isolated from terrestrial samples of the Antarctic. On the basis of 16S rRNA gene sequence analyses, the two Antarctic strains shared 97.7 % sequence similarity and showed moderate relationships to the genera Chryse...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.63273-0

    authors: Yi H,Yoon HI,Chun J

    更新日期:2005-01-01 00:00:00

  • Methanosarcina subterranea sp. nov., a methanogenic archaeon isolated from a deep subsurface diatomaceous shale formation.

    abstract::A methanogenic archaeon, strain HC-2(T), was isolated from a deep diatomaceous shale formation. The strain grew on methanol, monomethylamine, dimethylamine, trimethylamine and dimethylsulphide, but not on acetate, H2/CO2, formate, 2-propanol, 2-butanol or cyclopentanol. Cells were Gram-stain-negative, non-motile, and ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.000072

    authors: Shimizu S,Ueno A,Naganuma T,Kaneko K

    更新日期:2015-04-01 00:00:00

  • Methanobacterium lacus sp. nov., isolated from the profundal sediment of a freshwater meromictic lake.

    abstract::An autotrophic, hydrogenotrophic methanogen, designated strain 17A1(T), was isolated from the profundal sediment of the meromictic Lake Pavin, France. The cells of the novel strain, which were non-motile, Gram-staining-negative rods that measured 2-15 µm in length and 0.2-0.4 µm in width, grew as filaments. Strain 17A...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.034538-0

    authors: Borrel G,Joblin K,Guedon A,Colombet J,Tardy V,Lehours AC,Fonty G

    更新日期:2012-07-01 00:00:00

  • Sphingobacterium caeni sp. nov., isolated from activated sludge.

    abstract::The taxonomic status of a bacterium, strain DC-8(T), isolated from activated sludge, was determined using a polyphasic taxonomic approach. The cells of strain DC-8(T) were Gram-negative, non-motile, non-spore-forming and rod-shaped. The isolate grew at temperature range of 10-40 °C (optimum 30-35 °C), pH range of 5.0-...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.046987-0

    authors: Sun LN,Zhang J,Chen Q,He J,Li SP

    更新日期:2013-06-01 00:00:00

  • Glaciecola amylolytica sp. nov., an amylase-producing bacterium isolated from seawater.

    abstract::A Gram-stain-negative, aerobic, non-motile and coccus-shaped bacterium (THG-3.7T) was isolated from seawater. Growth occurred at 10-30 °C (optimum 25 °C), at pH 6-8 (optimum 7) and in the presence of 1-8 % (w/v) NaCl (optimum 4 %). Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.003222

    authors: Xiao YK,Yan ZF,Kim Y,Lee HM,Trinh H,Yang JE,Won KH,Yi TH,Kook M

    更新日期:2019-04-01 00:00:00

  • Azonexus hydrophilus sp. nov., a nifH gene-harbouring bacterium isolated from freshwater.

    abstract::Three Gram-negative, non-pigmented, rod-shaped, facultatively aerobic bacterial strains, designated d8-1T, d8-2 and IMCC1716, were isolated from a freshwater spring sample and a eutrophic freshwater pond. Based on characterization using a polyphasic approach, the three strains showed highly similar phenotypic, physiol...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.65434-0

    authors: Chou JH,Jiang SR,Cho JC,Song J,Lin MC,Chen WM

    更新日期:2008-04-01 00:00:00

  • Mesorhizobium waimense sp. nov. isolated from Sophora longicarinata root nodules and Mesorhizobium cantuariense sp. nov. isolated from Sophora microphylla root nodules.

    abstract::In total 14 strains of Gram-stain-negative, rod-shaped bacteria were isolated from Sophora longicarinata and Sophora microphylla root nodules and authenticated as rhizobia on these hosts. Based on the 16S rRNA gene phylogeny, they were shown to belong to the genus Mesorhizobium, and the strains from S. longicarinata w...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.000430

    authors: De Meyer SE,Wee Tan H,Heenan PB,Andrews M,Willems A

    更新日期:2015-10-01 00:00:00

  • Erythrobacter aquimixticola sp. nov., isolated from the junction between the ocean and a freshwater spring.

    abstract::A Gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, designated JSSK-14T, was isolated from the place where the ocean and a freshwater spring meet at Jeju island, South Korea. Strain JSSK-14T grew optimally at pH 7.0-8.0, at 30 °C and in the presence of 1.0-2.0 % (w/v) NaCl. A ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.002055

    authors: Park S,Jung YT,Choi SJ,Yoon JH

    更新日期:2017-08-01 00:00:00

  • Marinimicrobium locisalis sp. nov., isolated from a marine solar saltern, and emended description of the genus Marinimicrobium.

    abstract::A Gram-negative, motile and rod-shaped bacterial strain, designated ISL-43T, was isolated from a marine solar saltern of the Yellow Sea, Korea, and its taxonomic position was investigated by means of a polyphasic study. Strain ISL-43T grew optimally at pH 7.0-8.0 and 30 degrees C and in the presence of approximately 2...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.008458-0

    authors: Yoon JH,Kang SJ,Jung YT,Oh TK

    更新日期:2009-09-01 00:00:00

  • Aeromicrobium terrae sp. nov., isolated from a maize field.

    abstract::A polyphasic taxonomic approach was used to characterize a Gram-stain-positive bacterium, designated strain CC-CFT486T, isolated from soil sampled in a maize field in Taiwan. Cells of strain CC-CFT486T were short rods, motile with polar flagella, catalase-positive and oxidase-positive. Optimal growth occurred at 30 °С...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.004616

    authors: Lin SY,Tsai CF,Hameed A,Young CC

    更新日期:2021-01-05 00:00:00

  • Dyadobacter luticola sp. nov., isolated from a sewage sediment sample.

    abstract::A Gram-stain-negative, non-motile, aerobic bacterial strain, designated T17T was isolated from a sample of sewage sediment from a Busan park (Republic of Korea). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain T17T had the highest 16S rRNA gene sequence similarity to Dyadobacter soli KCTC 224...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.003178

    authors: Song Z,Song Y,Yu Y,Choi L,Wang G,Li M

    更新日期:2019-02-01 00:00:00

  • Halorubrum yunnanense sp. nov., isolated from a subterranean salt mine.

    abstract::Two halophilic archaeal strains, Q85(T) and Q86, were isolated from a subterranean salt mine in Yunnan, China. Cells were rod-shaped, Gram-stain-negative and motile. Colonies were red, smooth, convex and round (1.0-2.0 mm in diameter). The orthologous 16S rRNA and rpoB9 gene sequences of these two strains were almost ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.000605

    authors: Chen S,Liu HC,Zhao D,Yang J,Zhou J,Xiang H

    更新日期:2015-12-01 00:00:00

  • Thermus arciformis sp. nov., a thermophilic species from a geothermal area.

    abstract::Two aerobic, Gram-negative, non-motile, non-sporulating, yellow-pigmented bacteria, strains TH92(T) and TH91, were isolated from a hot spring located in Laibin, Guangxi, in the south-eastern geothermal area of China. The isolates grew at 40-77 degrees C (optimally at 70 degrees C) and at pH 6.0-9.5 (optimally at pH 7....

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.007690-0

    authors: Zhang XQ,Ying Y,Ye Y,Xu XW,Zhu XF,Wu M

    更新日期:2010-04-01 00:00:00

  • Rhodococcus qingshengii sp. nov., a carbendazim-degrading bacterium.

    abstract::A Gram-positive, aerobic, non-motile, mesophilic strain, djl-6(T), able to degrade carbendazim, was isolated from a carbendazim-contaminated soil sample from Jiangsu province, China. The taxonomic position of this isolate was analysed by using a polyphasic approach. Chemotaxonomic analysis including peptidoglycan type...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.65095-0

    authors: Xu JL,He J,Wang ZC,Wang K,Li WJ,Tang SK,Li SP

    更新日期:2007-12-01 00:00:00