Abstract:
:A cDNA for the human catalytic subunit (C beta) of cAMP-dependent protein kinase (PKA) has been cloned from a testis cDNA library. In the present study, we have determined the chromosomal localization of this gene using a cDNA for C beta as a probe. Southern blot analysis of genomic DNA from human/mouse cell hybrids revealed that the presence or absence of a 20-kb XbaI fragment, which hybridized with the C beta probe, was concordant with the presence of human chromosome 1. In situ hybridization to metaphase chromosome confirmed the somatic cell hybrid data and regionally mapped the C beta gene of PKA to the p36 band on chromosome 1.
journal_name
Hum Genetjournal_title
Human geneticsauthors
Simard J,Bérubé D,Sandberg M,Grzeschik KH,Gagné R,Hansson V,Jahnsen Tdoi
10.1007/BF02265292keywords:
subject
Has Abstractpub_date
1992-03-01 00:00:00pages
653-7issue
6eissn
0340-6717issn
1432-1203journal_volume
88pub_type
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