Glucocorticoid receptor upregulation during seawater adaptation in a euryhaline teleost, the tilapia (Oreochromis mossambicus).


:Cortisol is an important seawater (SW) osmoregulatory hormone in the Mozambique tilapia (Oreochromis mossambicus), a highly euryhaline cichlid able to live in environments ranging from fresh water (FW) to salinities well in excess of full-strength seawater. Previous studies indicate that cortisol may promote SW adaptation by increasing gill chloride cell differentiation, Na(+)/K(+)-ATPase activity and subsequent excretion of excess salt following seawater acclimation. Despite cortisol's widely accepted role as a SW-adapting hormone, cortisol receptor regulation during SW acclimation is not well understood. The purpose of these studies was to determine whether the intracellular glucocorticoid receptor (GR) might be regulated in a manner consistent with cortisol's actions in SW adaptation. Saturation radioligand binding assays were conducted on gill cytoplasm preparations from fish sampled 4 and 24h and 4 and 14 days after transfer from FW to 2/3 SW or FW (control). Affinity (K(d)) of the gill GR remained constant over the timecourse, while numbers of receptors (B(max)) in SW fish were significantly elevated compared with controls at 24h and 4 days after transfer. Plasma osmolality was higher in fish transferred to SW for 24h, 4 days, and 14 days compared with those animals moved to FW. Plasma cortisol levels and hepatic cortisol binding remained constant between SW and FW fish throughout the timecourse of the salinity challenge. These studies indicate that seawater acclimation is accompanied by a specific upregulation of intracellular GR numbers in gill tissue. The lack of increase in circulating cortisol following SW adaptation may reflect enhancement of clearance of the steroid. It appears that an increase in cortisol receptors, which is closely associated with the rise in blood osmotic pressure that accompanies SW exposure, is an important component of cortisol's ability to promote SW adaptation in the tilapia.


Gen Comp Endocrinol


Dean DB,Whitlow ZW,Borski RJ





Has Abstract


2003-06-01 00:00:00














  • Effects of estrogen and progesterone on serum prolactin and luteinizing hormone levels in ovariectomized turkeys (Meleagris gallopavo).

    abstract::Serum prolactin (PRL) and luteinizing hormone (LH) levels from mature female turkeys were determined following ovariectomy and daily subcutaneous injections for 4 or 9 days of 0.002-2.0 mg/kg estradiol benzoate (EB), 0.05-2.0 mg/kg progesterone (P), or their combinations. Serum PRL levels were increased (P less than 0...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: El Halawani ME,Silsby JL,Fehrer SC,Behnke EJ

    更新日期:1983-10-01 00:00:00

  • Control of vascular smooth muscle cell growth in fowl.

    abstract::In adult domestic fowl, angiotensin (ANG) receptors are present in the vascular smooth muscles (VSM) and in the endothelium, mediating vasorelaxation via endothelium-derived relaxing factor/cGMP. ANG II-induced relaxation is minor in chicks and becomes more marked as they mature but diminishes in adult birds, whereas ...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Shimada T,Fabian M,Yan HQ,Nishimura H

    更新日期:1998-10-01 00:00:00

  • In vitro effects of thyroid hormones on gonadotropin-induced estradiol-17 beta secretion by ovarian follicles of rainbow trout, Salmo gairdneri.

    abstract::Ovarian follicles isolated from rainbow trout during early exogenous vitellogenesis were used to study the in vitro effects of thyroid hormones on salmon gonadotropin (GtH)-induced estradiol-17 beta (E2) secretion. Triiodothyronine (T3) alone did not alter E2 secretion but T3 in the presence of GtH (0.5 micrograms/ml)...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Cyr DG,Eales JG

    更新日期:1988-01-01 00:00:00

  • Different actions of ecdysis-triggering hormone on the brain and ventral nerve cord of the hornworm, Manduca sexta.

    abstract::Ecdysis, or the shedding of the old cuticle, depends on coordinated stereotyped behaviors, regulated by a number of neuropeptides. In the hornworm, Manduca sexta, two neuropeptides interact, namely ecdysis-triggering hormone (ETH) and eclosion hormone. We looked at the effects of ETH in vivo and in vitro, on the brain...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Asuncion-Uchi M,El Shawa H,Martin T,Fuse M

    更新日期:2010-03-01 00:00:00

  • Ovarian development in red-sided garter snakes, Thamnophis sirtalis parietalis: relationship to mating.

    abstract::Female red-sided garter snakes (Thamnophis sirtalis parietalis) exhibit a complex array of behavioral patterns. Females may mate once, more than once, or not at all in the spring of the year, and a high percentage of females may mate in late summer before returning to winter hibernacula. The role of mating in the indu...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Whittier JM,Crews D

    更新日期:1986-01-01 00:00:00

  • Sexual dimorphism in the brain aromatase expression and activity, and in the central expression of other steroidogenic enzymes during the period of sex differentiation in monosex rainbow trout populations.

    abstract::Using genetic monosex male and female rainbow trout populations, the potential sex differences in the central expression of estrogen receptors (esr1, esr2a, esr2b), brain aromatase (cyp19a1b) and some other steroidogenic enzymes was studied over the period of sex differentiation (from 35 to 63 dpf: days post-fertiliza...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Vizziano-Cantonnet D,Anglade I,Pellegrini E,Gueguen MM,Fostier A,Guiguen Y,Kah O

    更新日期:2011-01-15 00:00:00

  • Neural remodelling in spiny lobster larvae is characterized by broad neuropeptide suppression.

    abstract::Neuropeptides are ancient endocrine components which have evolved to regulate many aspects of biology across the animal kingdom including behaviour, development and metabolism. To supplement current knowledge, we have utilized a transcriptome series describing larval development in the ornate spiny lobster, Panulirus ...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Hyde CJ,Nguyen T,Fitzgibbon QP,Elizur A,Smith GG,Ventura T

    更新日期:2020-08-01 00:00:00

  • How functional genomics and genetics complements insect endocrinology.

    abstract::Insects are the most abundant animal group on Earth and have been the subject of genetic and physiological studies since the beginning of the 19th century. The public interest in understanding their biology increased as many insects have proven to exert a severe impact on human welfare and the environment. To trigger ...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章,评审


    authors: Reumer A,Van Loy T,Clynen E,Schoofs L

    更新日期:2008-01-01 00:00:00

  • Changes in steroid profiles of the blue mussel Mytilus trossulus as a function of season, stage of gametogenesis, sex, tissue and mussel bed depth.

    abstract::This paper describes changes in the content of free steroid hormones e.g. testosterone (T), estradiol-17β (E2), estrone (E1) and estriol (E3) of Mytilus trossulus from the southern Baltic Sea as a function of season, stage of gametogenesis, sex, tissue (gonadal and somatic) and depth. The highest levels of T, E2, E1 a...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Smolarz K,Zabrzańska S,Konieczna L,Hallmann A

    更新日期:2018-04-01 00:00:00

  • Excretory fate of estradiol and progesterone in the African elephant (Loxodonta africana) and patterns of fecal steroid concentrations throughout the estrous cycle.

    abstract::We developed and validated a noninvasive method to quantify fecal estrogens and progestins as a tool for monitoring long-term ovarian activity in free-ranging African elephants. The lag times between iv injection of [(3)H]estradiol and [(14)C]progesterone and peak excretion of radioactivity in urine and feces were app...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Wasser SK,Papageorge S,Foley C,Brown JL

    更新日期:1996-05-01 00:00:00

  • High environmental ammonia exposure has developmental-stage specific and long-term consequences on the cortisol stress response in zebrafish.

    abstract::The capacity for early life environmental stressors to induce programming effects on the endocrine stress response in fish is largely unknown. In this study we determined the effects of high environmental ammonia (HEA) exposure on the stress response in larval zebrafish, assessed the tolerance of embryonic and larval ...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Williams TA,Bonham LA,Bernier NJ

    更新日期:2017-12-01 00:00:00

  • Autotransplantation of avian parathyroid glands: an animal model for studying parathyroid function.

    abstract::The parathyroid glands of chickens were autotransplanted and the return of parathyroid function following transplantation was determined. Parathyroidectomy (PTX) resulted in a marked hypocalcemia (5.2 +/- 0.2 mg/dl) 4 hr following PTX. Plasma calcium (PCa) had declined to 4.3 +/- 0.2 mg/dl 24 hr after PTX. Parathyroid...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Cole JA,Forte LR,Thorne PK,Poelling RE,Krause WJ

    更新日期:1989-12-01 00:00:00

  • Relationships between CB1 cannabinoid receptors and pituitary endocrine cells in Xenopus laevis: an immunohistochemical study.

    abstract::The distribution of the cannabinoid CB1 receptor and its relationships with individual endocrine cell types were investigated by immunohistochemistry in the anterior lobe of the Xenopus adenohypophysis. By use of a specific primary antibody raised in rabbit against the amino terminus of the rat CB1, we have found nume...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Cesa R,Guastalla A,Cottone E,Mackie K,Beltramo M,Franzoni MF

    更新日期:2002-01-01 00:00:00

  • Delayed access to feed alters gene expression associated with hormonal signaling, cellular differentiation, and protein metabolism in muscle of newly hatch chicks.

    abstract::Birds rely solely on utilization of the yolk sac as a means of nutritional support throughout embryogenesis and early post-hatch, before first feeding occurs. Newly hatched broiler (meat-type) chickens are frequently not given immediate access to feed, and this can result in numerous alterations to developmental proce...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Payne JA,Proszkowiec-Weglarz M,Ellestad LE

    更新日期:2020-06-01 00:00:00

  • Cloning and developmental expression of the cytochrome P450 aromatase gene (CYP19) in the European eel (Anguilla anguilla).

    abstract::To characterize the involvement of the aromatase gene during the process of sex determination in the European eel (Anguilla anguilla), the expression of its gonadal form was determined during various developmental stages. The cloned cDNA from the European eel gonad (EeCYP19) contains an open reading frame of 1539 bp, ...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Tzchori I,Degani G,Hurvitz A,Moav B

    更新日期:2004-09-15 00:00:00

  • Presence of thyroxine in eggs and changes in its content during early development of chum salmon, Oncorhynchus keta.

    abstract::In order to examine the role of thyroid hormones during salmonid development, techniques were developed for quantitative extraction of thyroxine from eggs, whole embryos, and alevins of chum salmon (Oncorhynchus keta) at various stages of development. Frozen eggs, embryos, alevins, or fry were homogenized in ice-cold ...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Tagawa M,Hirano T

    更新日期:1987-10-01 00:00:00

  • The dynamics of the steroidogenic response of perfused Xenopus testis explants to gonadotropins.

    abstract::The dynamics of androgen production by perfused Xenopus testis explants has been investigated under various gonadotropic stimulations. Androgens were measured in perfusate by radioimmunoassay. The behavior of hormones in the perfusion system was assessed. Pieces of testis were capable of producing androgens after more...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Boujard D,Joly J

    更新日期:1983-09-01 00:00:00

  • The distribution and physiological effects of three evolutionarily and sequence-related neuropeptides in Rhodnius prolixus: Adipokinetic hormone, corazonin and adipokinetic hormone/corazonin-related peptide.

    abstract::We have examined the distribution and physiological effects of three evolutionarily and sequence-related neuropeptides in Rhodnius prolixus. These neuropeptides, adipokinetic hormone (RhoprAKH), corazonin (CRZ) and adipokinetic hormone/corazonin-related peptide (RhoprACP) are present in distinct, non-overlapping neuro...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Patel H,Orchard I,Veenstra JA,Lange AB

    更新日期:2014-01-01 00:00:00

  • The role of 'mineralocorticoids' in teleost fish: relative importance of glucocorticoid signaling in the osmoregulation and 'central' actions of mineralocorticoid receptor.

    abstract::It has long been held that cortisol, a glucocorticoid in many vertebrates, performs glucocorticoid and mineralocorticoid actions in the teleost fish since it lacks aldosterone. However, in addition to the counterparts of tetrapod mineralocorticoid receptors (MRs), 11-deoxycorticosterone (DOC) has been recently identif...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章,评审


    authors: Takahashi H,Sakamoto T

    更新日期:2013-01-15 00:00:00

  • Comparative analysis of reproductive cycles in female Persian gazelle (Gazella subgutturosa subgutturosa) (Central Asia) and sand gazelle (Gazella subgutturosa marica) (Arabian Peninsula).

    abstract::This study was conducted at the Bukhara breeding centre (Ouzbekistan, Central Asia) on Persian gazelles (Gazella subgutturosa subgutturosa) and at Thumammah (Saudi Arabia) on sand gazelles (Gazella subgutturosa marica). Plasma prolactin (PRL) and progesterone were determined in groups of females. Because these females...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Sempéré AJ,Brown N,Pereladova OB,Bahloul K,Lacroix A,Soldatova N

    更新日期:2001-01-01 00:00:00

  • Acute exposure to physiological doses of triiodothyronine does not induce gonadal caspase 3 activity in goldfish in vitro.

    abstract::Seasonally spawning fish rely on a dynamic and complex hormonal interplay to regulate cycles of gonadal development and the regression. Thyroid hormones have been shown to be a key player during gonadal development, and can regulate the activity of a number of essential reproductive hormones. Apoptosis is a vital cell...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Allan ERO,Dores CB,Nelson ER,Habibi HR

    更新日期:2020-04-01 00:00:00

  • Real-time PCR analysis of cytochrome P450 aromatase expression in zebrafish: gene specific tissue distribution, sex differences, developmental programming, and estrogen regulation.

    abstract::In teleost fish, the predominant brain form of cytochrome P450 aromatase (P450aromB) is a neural marker of estrogen effect, and an entry point for studying the role of hormonal and environmental estrogens on neurodevelopment and neuroplasticity. As part of a project using zebrafish to investigate these issues, we deve...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Sawyer SJ,Gerstner KA,Callard GV

    更新日期:2006-06-01 00:00:00

  • Use of ethanol for preserving steroid and indoleamine hormones in bird plasma.

    abstract::Endocrinological research on wild animals inhabiting remote areas has been hampered by the need to store plasma samples at subzero temperatures. In an attempt to remedy this logistical issue, we here investigate the use of ethanol as an alternative to freezing for the preservation of steroid and indoleamine hormones i...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Goymann W,Schwabl I,Trappschuh M,Hau M

    更新日期:2007-01-15 00:00:00

  • Food, stress, and circulating testosterone: Cue integration by the testes, not the brain, in male zebra finches (Taeniopygia guttata).

    abstract::Food abundance is closely associated with reproductive readiness in vertebrates. Food scarcity can activate the hypothalamo-pituitary-adrenal axis, decrease sex steroid secretion, and dampen reproductive behavior. However, the mechanisms underlying these transient effects are unclear. Gonadotropin inhibitory hormone (...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Lynn SE,Perfito N,Guardado D,Bentley GE

    更新日期:2015-05-01 00:00:00

  • Thyroidectomy of house sparrows (Passer domesticus) prevents photo-induced testicular growth but not the increased hypothalamic gonadotrophin-releasing hormone.

    abstract::Thyroidectomy of starlings (Sturnus vulgaris) prevents the decrease in hypothalamic gonadotrophin-releasing hormone (GnRH) which normally occurs at the onset of photorefractoriness. To extend this observation to another species, changes in testicular mass, bill colour, moult, and hypothalamic GnRH content were monitor...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Dawson A

    更新日期:1998-05-01 00:00:00

  • Plasma estradiol and progesterone concentrations during the female reproductive cycle in a highly placentotrophic viviparous lizard, Mabuya sp.

    abstract::The neotropical genus Mabuya are obligate placentotrophic viviparous lizards, which have a short vitellogenesis that produces microlecithal oocytes and a prolonged time of gestation (9 to 10 months). The hormonal control of female reproductive activity during follicular growth and pregnancy has not been studied, altho...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Barbosa-Moyano H,Rodríguez-Chaparro S,Santos RLSR,Ramírez-Pinilla MP

    更新日期:2020-09-01 00:00:00

  • Hormone cross-regulation in the tadpole brain: developmental expression profiles and effect of T3 exposure on thyroid hormone- and estrogen-responsive genes in Rana pipiens.

    abstract::During metamorphosis, the tadpole neuroendocrine brain is a major target for the organisational effects of hormones acting via both endocrine feedback mechanisms and local hormone production. While the receptor-mediated actions of thyroid hormones in brain development have been well described, there is evidence that t...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Hogan NS,Crump KL,Duarte P,Lean DR,Trudeau VL

    更新日期:2007-10-01 00:00:00

  • Parathyroid hormone-related protein is a factor in normal fish pituitary.

    abstract::Using antibodies to the amino-terminal region of human parathyroid hormone-related protein (PTHrP) we have demonstrated PTHrP immunoreactivity in pituitaries and plasma of the sea bream (Sparus aurata). Pituitary cells at two distinct locations contained immunodetectable PTHrP; an anterior group in the rostral pars di...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Danks JA,Devlin AJ,Ho PM,Diefenbach-Jagger H,Power DM,Canario A,Martin TJ,Ingleton PM

    更新日期:1993-11-01 00:00:00

  • Endocrine changes in photostimulated willow ptarmigan (Lagopus lagopus lagopus) and Svalbard ptarmigan (Lagopus mutus hyperboreus).

    abstract::Changes in plasma luteinizing hormone (LH), testosterone, thyroxine (T4), and triiodothyronine (T3) and the height of supraorbital combs were compared in captive willow ptarmigan (Lagopus lagopus lagopus) and Svalbard ptarmigan (Lagopus mutus hyperboreus) exposed to an artificial annual cycle of daylength simulating t...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Stokkan KA,Sharp PJ,Dunn IC,Lea RW

    更新日期:1988-04-01 00:00:00

  • The effects of age, season, and gender on serum cortisol levels in the tammar wallaby, Macropus eugenii.

    abstract::Serum cortisol levels were measured in a total of 73 tammar wallabies maintained in a captive population at Macquarie University, NSW, Australia. Previous studies of corticosteroids in marsupials have generally involved low sample numbers, a diverse array of analytical techniques, and a variety of sampling conditions....

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: McKenzie S,Deane EM

    更新日期:2003-10-01 00:00:00