Chromatin fiber folding: requirement for the histone H4 N-terminal tail.

abstract：:Ribosomes are dynamic complexes responsible for translating the genetic information encoded in mRNAs to proteins. The accuracy of this process is vital to the survival of an organism, and is often compromised by translational miscoding agents. Aminoglycosides are a group of miscoding agents that bind to the ribosome a...

journal_title：Journal of molecular biology

authors： Jerinic O,Joseph S

更新日期：2000-12-15 00:00:00

abstract：:The mRNA-capping process starts with the conversion of a 5'-triphosphate end into a 5'-diphosphate by an RNA triphosphatase, followed by the addition of a guanosine monophosphate unit in a 5'-5' phosphodiester bond by a guanylyltransferase. Methyltransferases are involved in the third step of the process, transferring...

journal_title：Journal of molecular biology

authors： Bollati M,Milani M,Mastrangelo E,Ricagno S,Tedeschi G,Nonnis S,Decroly E,Selisko B,de Lamballerie X,Coutard B,Canard B,Bolognesi M

更新日期：2009-01-09 00:00:00

abstract：:Protein trafficking pathways to plastids are directed by N-terminal targeting peptides. In plants this consists of a relatively simple transit peptide, while in organisms with secondary plastids (which reside within the endomembrane system) a signal peptide is appended to the transit peptide. Despite amino acid compos...

journal_title：Journal of molecular biology

authors： Patron NJ,Waller RF,Archibald JM,Keeling PJ

更新日期：2005-05-13 00:00:00

abstract：:The gene sup61+, which codes for the essential Saccharomyces cerevisiae tRNA(SerUCG), is the only single-copy tRNA gene in this organism know to contain an intron. To assess the role of this intron in tRNA gene expression, an intron-deleted sup61+ gene was constructed in vitro and introduced into the yeast genome. Iso...

journal_title：Journal of molecular biology

authors： Ho CK,Abelson J

更新日期：1988-08-05 00:00:00

abstract：:TF1 is a member of the family of type II DNA-binding proteins, which also includes the bacterial HU proteins and the Escherichia coli integration host factor (IHF). Distinctive to TF1, which is encoded by the Bacillus subtilis bacteriophage SPO1, is its preferential binding to DNA in which thymine is replaced by 5-hyd...

journal_title：Journal of molecular biology

authors： Grove A,Galeone A,Mayol L,Geiduschek EP

更新日期：1996-07-12 00:00:00

abstract：:Antisense RNAs interact with their complementary target RNAs as folded structures. The formation of early binding intermediates is the most important step in determining the overall rates of stable complex formation in vitro and the efficiency of control in vivo. In the case of CopA and CopT (antisense/target RNA pair...

journal_title：Journal of molecular biology

authors： Nordgren S,Slagter-Jäger JG,Wagner GH

更新日期：2001-07-27 00:00:00

abstract：:The role of an 18-residue ion-pair network, that is present in the glutamate dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus, in conferring stability to other, less stable homologous enzymes, has been studied by introducing four new charged amino acid residues into the subunit interface of glutam...

journal_title：Journal of molecular biology

authors： Lebbink JH,Knapp S,van der Oost J,Rice D,Ladenstein R,de Vos WM

更新日期：1999-06-04 00:00:00

abstract：:Reconstitution of liver fluke (Dicrocoelium dendriticum) apo-hemoglobin with hemins selectively deuterated at specific positions has permitted the assignment of several heme resonances in the proton nuclear magnetic resonance spectrum of the Met-aquo and Met-cyano forms of the holoprotein. It was established that in t...

journal_title：Journal of molecular biology

authors： Lecomte JT,La Mar GN,Smit JD,Winterhalter KH,Smith KM,Langry KC,Leung HK

更新日期：1987-09-05 00:00:00

abstract：:Monoclinic P2(1) crystals of the bacteriophage phi X174 have been incubated with calcium ions (Ca2+) and the induced structural conformational changes studied to 3 A resolution with X-ray crystallographic methods. Three different types of Ca2+ binding sites have been located within the asymmetric unit of the virion. T...

journal_title：Journal of molecular biology

authors： Ilag LL,McKenna R,Yadav MP,BeMiller JN,Incardona NL,Rossmann MG

更新日期：1994-12-02 00:00:00

abstract：:The interactions between the complement receptor type 2 (CR2) and the C3 complement fragments C3d, C3dg, and iC3b are essential for the initiation of a normal immune response. A crystal-derived structure of the two N-terminal short consensus repeat (SCR1-2) domains of CR2 in complex with C3d has previously been elucid...

journal_title：Journal of molecular biology

authors： Shaw CD,Storek MJ,Young KA,Kovacs JM,Thurman JM,Holers VM,Hannan JP

更新日期：2010-12-10 00:00:00

abstract：:Crystals of a relatively unspecific NAD(P)H dehydrogenase from human erythrocytes suitable for X-ray analysis have been grown. They belong to space group P4(1)2(1)2 or its enantiomorph P4(3)2(1)2 with unit cell dimensions: a = b = 79.3 A and c = 38.1 A. The asymmetric unit contains one molecule of Mr 18,000. ...

journal_title：Journal of molecular biology

authors： Pai EF

更新日期：1984-04-25 00:00:00

abstract：:The domain organization of the zymogen subunits of the first component of human complement C1s, C1r2 and the complex C1s-C1r2-C1s was studied by electron microscopy. In the absence of Ca2+, monomeric C1s was visualized as a dumb-bell-shaped molecule consisting of two globular domains (center-to-center distance 11 nm) ...

journal_title：Journal of molecular biology

authors： Weiss V,Fauser C,Engel J

更新日期：1986-06-05 00:00:00

abstract：:RIG-I-like receptors (RLRs) are nucleic acid sensors that activate antiviral innate immune response. These molecules recognize diverse non-self RNA substrates and are antagonized by several viral inhibitors. We performed an evolutionary analysis of RLR genes (RIG-I, MDA5, and LGP2) in mammals. Results indicated that p...

journal_title：Journal of molecular biology

authors： Cagliani R,Forni D,Tresoldi C,Pozzoli U,Filippi G,Rainone V,De Gioia L,Clerici M,Sironi M

更新日期：2014-03-20 00:00:00

abstract：:Previously we have shown that nuclear extracts from mouse cells contain a heterogeneous group of polypeptides (p65, p80, p90, p100) which form distinct DNA-protein complexes on the 18 base-pair sequence element (termed Sal-box), which constitutes the murine rDNA transcription termination signal. These distinct protein...

journal_title：Journal of molecular biology

authors： Smid A,Finsterer M,Grummt I

更新日期：1992-10-05 00:00:00

abstract：:The thermodynamic hypothesis of Anfinsen postulates that structures and stabilities of globular proteins are determined by their amino acid sequences. Chain topology, however, is known to influence the folding reaction, in that motifs with a preponderance of local interactions typically fold more rapidly than those wi...

journal_title：Journal of molecular biology

authors： Hills RD Jr,Kathuria SV,Wallace LA,Day IJ,Brooks CL 3rd,Matthews CR

更新日期：2010-04-30 00:00:00

abstract：:We have applied a search strategy for determining the optimal packing of protein secondary structure elements to the rotational positioning of the seven transmembrane helices of bacteriorhodopsin. The search is based on the assumption that the relative orientations of the helices within the bundle are conditioned prin...

journal_title：Journal of molecular biology

authors： Tuffery P,Etchebest C,Popot JL,Lavery R

更新日期：1994-03-04 00:00:00

abstract：:The rotavirus double-layered particle (DLP) is a molecular machine that transcribes 11 genomic segments of double-stranded RNA into full-length mRNA segments during viral replication. DLPs from the human Wa strain of virus, belonging to subgroup II (SG II), possess a significantly reduced level of transcriptase activi...

journal_title：Journal of molecular biology

authors： Greig SL,Berriman JA,O'Brien JA,Taylor JA,Bellamy AR,Yeager MJ,Mitra AK

更新日期：2006-02-10 00:00:00

abstract：:Siderophores are small-molecule iron chelators that many bacteria synthesize and secrete in order to survive in iron-depleted environments. Biosynthesis of enterobactin, the Escherichia coli catecholate siderophore, requires adenylation of 2,3-dihydroxybenzoic acid (2,3-DHB) by the cytoplasmic enzyme EntE. The DHB-AMP...

journal_title：Journal of molecular biology

authors： Khalil S,Pawelek PD

更新日期：2009-10-30 00:00:00

abstract：:This analysis takes an in-depth look into the difficulties encountered by automatic methods for domain decomposition from three-dimensional structure. The analysis involves a multi-faceted set of criteria including the integrity of secondary structure elements, the tendency toward fragmentation of domains, domain boun...

journal_title：Journal of molecular biology

authors： Holland TA,Veretnik S,Shindyalov IN,Bourne PE

更新日期：2006-08-18 00:00:00

abstract：:A DNA (cytosine)-5-methyltransferase from Haemophilus aegyptius (M.Hae III), which catalyzes methyl transfer from S-adenosyl-L-methionine to DNA, has been crystallized as a covalent complex with a suicide oligonucleotide substrate. Crystals of the co-complex were grown by vapor diffusion with hanging droplets, using p...

journal_title：Journal of molecular biology

authors： Reinisch KM,Chen L,Verdine GL,Lipscomb WN

更新日期：1994-05-13 00:00:00

abstract：:Despite the fact that DNA polymerases have been investigated for many years and are commonly used as tools in a number of molecular biology assays, many details of the kinetic mechanism they use to catalyze DNA synthesis remain unclear. Structural and kinetic studies have characterized a rapid, pre-catalytic open-to-c...

journal_title：Journal of molecular biology

authors： Xu C,Maxwell BA,Suo Z

更新日期：2014-08-12 00:00:00

abstract：:Contact-dependent growth inhibition (CDI) is a widespread mechanism of inter-bacterial competition mediated by the CdiB/CdiA family of two-partner secretion proteins. CdiA effectors carry diverse C-terminal toxin domains (CdiA-CT), which are delivered into neighboring target cells to inhibit growth. CDI(+) bacteria al...

journal_title：Journal of molecular biology

authors： Morse RP,Willett JL,Johnson PM,Zheng J,Credali A,Iniguez A,Nowick JS,Hayes CS,Goulding CW

更新日期：2015-11-20 00:00:00

abstract：:We have applied the method of simulated annealing to the refinement of the 3 A resolution crystal structure of the influenza virus hemagglutinin glycoprotein, using the program X-PLOR. Two different methods were introduced into X-PLOR to treat the non-crystallographic symmetry present in this and in other crystal stru...

journal_title：Journal of molecular biology

authors： Weis WI,Brünger AT,Skehel JJ,Wiley DC

更新日期：1990-04-20 00:00:00

abstract：:DnaB helicase is a ring-shaped hexamer that unwinds DNA at a replication fork. To understand how this protein interacts with DNA during unwinding, DnaB from Thermus aquaticus was incubated with chemically modified forked-duplex DNA substrates and the unwinding rates were measured. Unwinding was inhibited by modificati...

journal_title：Journal of molecular biology

authors： Kaplan DL

更新日期：2000-08-11 00:00:00

abstract：:A mutant of bovine pancreatic trypsin inhibitor (BPTI) has been constructed and expressed in Escherichia coli in order to probe the kinetic and structural consequences of truncating the binding loop residues to alanine. In addition to two such mutations (Thr11Ala and Pro13Ala), it has a conservative Lys15Arg substitut...

journal_title：Journal of molecular biology

authors： Czapinska H,Otlewski J,Krzywda S,Sheldrick GM,Jaskólski M

更新日期：2000-02-04 00:00:00

abstract：:Tankyrases are recently discovered proteins implicated in many important functions in the cell including telomere homeostasis and mitosis. Tankyrase modulates the activity of target proteins through poly(ADP-ribosyl)ation, and here we report the structure of the catalytic poly(ADP-ribose) polymerase (PARP) domain of h...

journal_title：Journal of molecular biology

authors： Lehtiö L,Collins R,van den Berg S,Johansson A,Dahlgren LG,Hammarström M,Helleday T,Holmberg-Schiavone L,Karlberg T,Weigelt J

更新日期：2008-05-23 00:00:00

abstract：:Interactions among Bcl-2 family proteins are important for regulating apoptosis. Prosurvival members of the family interact with proapoptotic BH3 (Bcl-2-homology-3)-only members, inhibiting execution of cell death through the mitochondrial pathway. Structurally, this interaction is mediated by binding of the alpha-hel...

journal_title：Journal of molecular biology

authors： Dutta S,Gullá S,Chen TS,Fire E,Grant RA,Keating AE

更新日期：2010-05-21 00:00:00

abstract：:The resonances of all the base protons and most of the sugar protons in both strands of the 17 base-pair OR3 operator of the phage lambda, and of the vC3 single base-pair mutant, have been assigned using two-dimensional nuclear magnetic resonance methods. The chemical shift and nuclear Overhauser effect data for these...

journal_title：Journal of molecular biology

authors： Wemmer DE,Chou SH,Reid BR

更新日期：1984-11-25 00:00:00

abstract：:In the crowded cell, a strong selective pressure operates on the proteome to limit the competition between functional and non-functional protein-protein interactions. We developed an original theoretical framework in order to interrogate how this competition constrains the behavior of proteins with respect to their pa...

journal_title：Journal of molecular biology

authors： Schweke H,Mucchielli MH,Sacquin-Mora S,Bei W,Lopes A

更新日期：2020-02-14 00:00:00

abstract：:The excessive activity of matrix metalloproteinases (MMPs) contributes to pathological processes such as arthritis, tumor growth and metastasis if not balanced by the tissue inhibitors of metalloproteinases (TIMPs). In arthritis, the destruction of fibrillar (type II) collagen is one of the hallmarks, with MMP-1 (coll...

journal_title：Journal of molecular biology

authors： Maskos K,Lang R,Tschesche H,Bode W

更新日期：2007-03-02 00:00:00