Direct comparison of enzyme histochemical and immunohistochemical methods to localize an enzyme.


:Immunohistochemical localization of enzymes is compared directly with localization of enzyme activity with (catalytic) enzyme histochemical methods. The two approaches demonstrate principally different aspects of an enzyme. The immunohistochemical method localizes the enzyme protein whether it is active or not whereas the catalytic method visualizes the functionally active enzyme only. Furthermore, enzyme histochemical methods appear to be highly sensitive in comparison with immunohistochemical methods. Finally, the catalytic methods are simple and last 5-60 min at room temp, whereas immunohistochemical methods take hours at least. It can be concluded that enzyme histochemical methods are the methods of choice to localize enzymes because they detect the functional activity of an enzyme, they are relatively simple and their sensitivity is high.


Mar Environ Res


Van Noorden CJ





Has Abstract


2002-09-01 00:00:00














  • Short term impact of artisanal dredges in a Patagonian mussel fishery: Comparisons with commercial diving and control sites.

    abstract::Mussels in the San Matías Gulf fishery are targeted using artisanal dredges and diving. The main objective of this study was to assess the direct impact of artisanal dredging on the biota and sediments, and to compare the composition of the catches and the individual damage induced by fishing between dredging and comm...

    journal_title:Marine environmental research

    pub_type: 杂志文章


    authors: Narvarte M,González R,Medina A,Avaca MS,Ginsberg S,Aliotta S

    更新日期:2012-02-01 00:00:00

  • The effects of shoreface nourishments on Spisula and scoters in The Netherlands.

    abstract::The coast of The Netherlands is protected by nourishing sand. Generally, two different techniques are used, beach nourishment and shoreface nourishment. The latter technique supplies sand at a water depth of about 5-8m in the surf zone, and has been used on a regular basis since 1997 with increasing volumes since 2001...

    journal_title:Marine environmental research

    pub_type: 杂志文章


    authors: Baptist MJ,Leopold MF

    更新日期:2009-07-01 00:00:00

  • The expression pattern of hsp70 plays a critical role in thermal tolerance of marine demersal fish: Multilevel responses of Paralichthys olivaceus and its hybrids (P. olivaceus ♀ × P. dentatus ♂) to chronic and acute heat stress.

    abstract::Ocean warming has multifaceted impacts on marine organisms. This study investigated the different responses of Paralichthys olivaceus and the hybrids (P. olivaceus ♀ × P. dentatus ♂) to chronic and acute heat stress. By comparing their survival, behavioural and histological changes, we found that the hybrids possess a...

    journal_title:Marine environmental research

    pub_type: 杂志文章


    authors: Liu Y,Ma D,Zhao C,Xiao Z,Xu S,Xiao Y,Wang Y,Liu Q,Li J

    更新日期:2017-08-01 00:00:00

  • Endocrine disruption in a marine amphipod? Field observations of intersexuality and de-masculinisation.

    abstract::Intersexuality has been found in both males and females of the marine/estuarine amphipod, Echinogammarus marinus, at polluted and reference sites in East Scotland. Polluted sites had significantly more intersex specimens than reference sites, however the cause of intersexuality is unclear. Discriminant analysis of mor...

    journal_title:Marine environmental research

    pub_type: 杂志文章


    authors: Ford AT,Fernandes TF,Rider SA,Read PA,Robinson CD,Davies IM

    更新日期:2004-08-01 00:00:00

  • Changes in benthic communities along a presumed pollution gradient in Vancouver Harbour.

    abstract::Samples of macrobenthic organisms were obtained at seven stations on a presumed pollution gradient from the head of Vancouver Harbour through to outer Howe Sound. Polychaetes (83 apparent species) and molluscs (43 apparent species) were the most abundant faunal groups numerically (44.8 and 47.9%, respectively). Mollus...

    journal_title:Marine environmental research

    pub_type: 杂志文章


    authors: Je JG,Belan T,Levings C,Koo BJ

    更新日期:2004-02-01 00:00:00

  • Monitoring PAH contamination in the field (South west Iberian Peninsula): biomonitoring using fluorescence spectrophotometry and physiological assessments in the shore crab Carcinus maenas (L.) (Crustacea: Decapoda).

    abstract::Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous pollutants of the marine environment, arising predominantly from petrochemical contamination and pyrogenic sources. A biomarker of PAH exposure was employed in a field study (South West, Spain) in both captured (indigenous) and deployed (caged) shore crabs (Carcin...

    journal_title:Marine environmental research

    pub_type: 杂志文章


    authors: Dissanayake A,Bamber SD

    更新日期:2010-07-01 00:00:00

  • Characterizing estuarine plume discharge into the coastal ocean using fatty acid biomarkers and pigment analysis.

    abstract::The transformation of estuaries by human activities continues to alter the biogeochemical balance of the coastal ocean. The disruption of this balance can negatively impact the provision of goods and services, including fisheries, commerce and transportation, recreation and esthetic enjoyment. Here we examine a link, ...

    journal_title:Marine environmental research

    pub_type: 杂志文章


    authors: Fischer AM,Ryan JP,Levesque C,Welschmeyer N

    更新日期:2014-08-01 00:00:00

  • Comparison of artificially weathered Macondo oil with field samples and evidence that weathering does not increase environmental acute toxicity.

    abstract::Macondo source oils and artificially weathered oil residues from 150 °C+ to 300 °C+, including artificially photo oxidized oils, were prepared and used for generating low energy water accommodated fractions (LE-WAFs) in order to assess the impact of oil weathering on WAF chemistry composition and toxicity to marine or...

    journal_title:Marine environmental research

    pub_type: 杂志文章


    authors: Faksness LG,Altin D,Størseth TR,Nordtug T,Hansen BH

    更新日期:2020-05-01 00:00:00

  • Assessment of the levels of polycyclic aromatic hydrocarbons and organochlorine contaminants in bottlenose dolphins (Tursiops truncatus) from the Eastern Atlantic Ocean.

    abstract::The concentrations of 18 polychlorinated biphenyls (PCBs), 23 organochlorine pesticides (OCPs), and 16 polycyclic aromatic hydrocarbons (PAHs) were determined in the blubber and liver of 27 bottlenose dolphins (Tursiops truncatus) stranded along the Canary Islands coasts from 1997 to 2011. DDTs (mean of 60,960 and 445...

    journal_title:Marine environmental research

    pub_type: 杂志文章


    authors: García-Álvarez N,Boada LD,Fernández A,Zumbado M,Arbelo M,Sierra E,Xuriach A,Almunia J,Camacho M,Luzardo OP

    更新日期:2014-09-01 00:00:00

  • Transcriptional and post-transcriptional response of drug-metabolizing enzymes to PAHs contamination in red mullet (Mullus barbatus, Linnaeus, 1758): a field study.

    abstract::Aim of this study was to evaluate the responsiveness of red mullet (Mullus barbatus) liver detoxification enzymes to PAHs at transcriptional and post-transcriptional levels in the field. Fish were captured in the north-eastern Adriatic Sea, close to an oil refinery. Sixteen PAHs (EPA) were determined in sediments and ...

    journal_title:Marine environmental research

    pub_type: 杂志文章


    authors: Della Torre C,Corsi I,Nardi F,Perra G,Tomasino MP,Focardi S

    更新日期:2010-07-01 00:00:00

  • Coral reefs as the first line of defense: Shoreline protection in face of climate change.

    abstract::Coral reefs are responsible for a wide array of ecosystem services including shoreline protection. However, the processes involved in delivering this particular service have not been fully understood. The objective of the present review was to compile the main results in the literature regarding the study of shoreline...

    journal_title:Marine environmental research

    pub_type: 杂志文章,评审


    authors: Elliff CI,Silva IR

    更新日期:2017-06-01 00:00:00

  • Contrasting effects of a classic Nrf2 activator, tert-butylhydroquinone, on the glutathione-related antioxidant defenses in Pacific oysters, Crassostrea gigas.

    abstract::Nrf2 is a well-known transcription factor controlling a number of antioxidant defense-related genes, which is understudied in bivalves. In this study, oysters Crassostrea gigas were exposed for 24, 48 and 96 h to 10 or 30 μM tert-butylhydroquinone (tBHQ), a classic Nrf2 activator. At 96 h, a clear induction of GSH-rel...

    journal_title:Marine environmental research

    pub_type: 杂志文章


    authors: Danielli NM,Trevisan R,Mello DF,Fischer K,Deconto VS,Bianchini A,Bainy ACD,Dafre AL

    更新日期:2017-09-01 00:00:00

  • Effects of novobiocin and methotrexate on the benthic amphipod Ampelisca brevicornis exposed to spiked sediments.

    abstract::The marine amphipod Ampelisca brevicornis was used as model organism of benthic macrofauna to assess the possible adverse effects of pharmaceuticals bound to sediments. Organisms were exposed to sediment spiked with novobiocin (NOV) and methotrexate (MTX) for 10 days in order to estimate the acute toxicity (lethal eff...

    journal_title:Marine environmental research

    pub_type: 杂志文章


    authors: Moreira LB,Maranho LA,Baena-Nogueras RM,Lara-Martín PA,Martín-Díaz ML

    更新日期:2016-12-01 00:00:00

  • Assessment of putative endocrine disrupters in an in vivo crustacean assay and an in vitro insect assay.

    abstract::Concern over endocrine disrupters in coastal ecosystems has stimulated global efforts to understand their potential impacts on fish and invertebrate communities. Given that marine crustaceans are sensitive to the effects of alkylphenols and other xenobiotics, we are currently investigating whether these responses may ...

    journal_title:Marine environmental research

    pub_type: 杂志文章


    authors: Pounds NA,Hutchinson TH,Williams TD,Whiting P,Dinan L

    更新日期:2002-09-01 00:00:00

  • Sex ratios of migrating southern hemisphere humpback whales: A new sentinel parameter of ecosystem health.

    abstract::Southern hemisphere humpback whales have evolved energetically demanding capital breeding and migratory life-history behaviours. It has been hypothesised that not all individuals of a population participate in the seasonal migration each year, or only undertake partial migrations. Given the cost of migration and repro...

    journal_title:Marine environmental research

    pub_type: 杂志文章


    authors: Druskat A,Ghosh R,Castrillon J,Bengtson Nash SM

    更新日期:2019-10-01 00:00:00

  • Spiculosiphon oceana (Foraminifera) a new bio-indicator of acidic environments related to fluid emissions of the Zannone Hydrothermal Field (central Tyrrhenian Sea).

    abstract::The new record of a shallow-water submarine hydrothermal field (<150 m w.d.) in the western Mediterranean Sea (Tyrrhenian Sea, Italy) allows us to study CO2 fluid impact on benthic foraminifers. Benthic foraminifers calcification process is sensitive to ocean acidification and to local chemical and physical parameters...

    journal_title:Marine environmental research

    pub_type: 杂志文章


    authors: Di Bella L,Ingrassia M,Frezza V,Chiocci FL,Pecci R,Bedini R,Martorelli E

    更新日期:2018-05-01 00:00:00

  • Drivers of shell growth of the bivalve, Callista chione (L. 1758) - Combined environmental and biological factors.

    abstract::Seasonal shell growth patterns were analyzed using the stable oxygen and carbon isotope values of live-collected specimens of the bivalve Callista chione from two sites in the Adriatic Sea (Pag and Cetina, Croatia). Micromilling was performed on the shell surface of three shells per site and shell oxygen isotopes of t...

    journal_title:Marine environmental research

    pub_type: 杂志文章


    authors: Purroy A,Milano S,Schöne BR,Thébault J,Peharda M

    更新日期:2018-03-01 00:00:00

  • Environmental consequence analyses of fish farm emissions related to different scales and exemplified by data from the Baltic--a review.

    abstract::The aim of this work is to review studies to evaluate how emissions from fish cage farms cause eutrophication effects in marine environments. The focus is on four different scales: (i) the conditions at the site of the farm, (ii) the local scale related to the coastal area where the farm is situated, (iii) the regiona...

    journal_title:Marine environmental research

    pub_type: 杂志文章,评审


    authors: Gyllenhammar A,Håkanson L

    更新日期:2005-08-01 00:00:00

  • Contrasting effects of constant and fluctuating pCO2 conditions on the exercise physiology of coral reef fishes.

    abstract::Ocean acidification (OA) is predicted to affect the physiology of some fishes. To date, most studies have investigated this issue using stable pCO2 levels based on open ocean projections. Yet, most shallow, nearshore systems experience temporal and spatial pCO2 fluctuations. For example, pCO2 on coral reefs is highest...

    journal_title:Marine environmental research

    pub_type: 杂志文章


    authors: Hannan KD,McMahon SJ,Munday PL,Rummer JL

    更新日期:2021-01-01 00:00:00

  • Genotoxic, cytotoxic and developmental effects of tributyltin oxide (TBTO): an integrated approach to the evaluation of the relative sensitivities of two marine species.

    abstract::Adopting an integrated approach the potential genotoxic, cytotoxic and developmental effects of tributyltin oxide (TBTO), a known endocrine-disrupting agent for neogastropods, have been evaluated in two ecologically relevant invertebrates: Mytilus edulis (blue mussel) and Platynereis dumerilli (rag worm). Following de...

    journal_title:Marine environmental research

    pub_type: 杂志文章


    authors: Jha AN,Hagger JA,Hill SJ,Depledge MH

    更新日期:2000-07-01 00:00:00

  • Exoskeletal deformities in Palaemonidae: are they a threat to survival?

    abstract::Shrimps of the Palaemon genus have been affected for over 15 years by a problem of exoskeletal deformities, particularly in the Gironde estuary (France). Given the large numbers of individuals affected, this study focus on a better description of the phenomenon to estimate its impact on shrimps. This study, on Palaemo...

    journal_title:Marine environmental research

    pub_type: 杂志文章


    authors: Béguer M,Feuillassier L,Elie P,Boët P,Girardin M

    更新日期:2010-04-01 00:00:00

  • A multibiomarker approach in the clam Ruditapes decussatus to assess the impact of pollution in the Ria Formosa lagoon, South Coast of Portugal.

    abstract::The Ria Formosa lagoon is an ecosystem whose water quality reflects the anthropogenic influence upon the surrounding areas. In this lagoon, the clam Ruditapes decussatus has a great economical importance and has been widely used as a biomonitor. A multibiomarker approach (δ-aminolevulinic acid dehydratase, metallothio...

    journal_title:Marine environmental research

    pub_type: 杂志文章


    authors: Cravo A,Pereira C,Gomes T,Cardoso C,Serafim A,Almeida C,Rocha T,Lopes B,Company R,Medeiros A,Norberto R,Pereira R,Araújo O,Bebianno MJ

    更新日期:2012-04-01 00:00:00

  • Sea urchin larvae show resilience to ocean acidification at the time of settlement and metamorphosis.

    abstract::Extensive research has shown that the early life stages of marine organisms are sensitive to ocean acidification (OA). Less is known, however, on whether larval settlement and metamorphosis may be affected, or by which mechanisms. These are key processes in the life cycle of most marine benthic organisms, since they m...

    journal_title:Marine environmental research

    pub_type: 杂志文章


    authors: Espinel-Velasco N,Agüera A,Lamare M

    更新日期:2020-07-01 00:00:00

  • Two CYP3A-like genes in the marine mussel Mytilus edulis: mRNA expression modulation following short-term exposure to endocrine disruptors.

    abstract::Members of the vertebrate CYP3A subfamily are involved in the metabolism of steroids and a wide range of xenobiotics. In this study two CYP3A-like mRNAs have been isolated from the mussel (Mytilus edulis), and their seasonal expression profile and modulation by estrogens examined. Sexual dimorphism of CYP3A-like mRNA ...

    journal_title:Marine environmental research

    pub_type: 杂志文章


    authors: Cubero-Leon E,Puinean AM,Labadie P,Ciocan C,Itoh N,Kishida M,Osada M,Minier C,Hill EM,Rotchell JM

    更新日期:2012-03-01 00:00:00

  • Genetic polymorphism and its potential relation to environmental stress in five populations of the European flounder Platichthys flesus, along the French Atlantic coast.

    abstract::In this study, new DNA markers were explored for the flounder Platichthys flesus. cDNA and genomic sequences of the genes encoding the glyceraldehyde-3-phosphate-deshydrogenase (GAPDH), the cytosolic creatine kinase (CK), the prostaglandin D synthase (PGDS) and the betaine homocysteine methyltransferase (BHMT) were ch...

    journal_title:Marine environmental research

    pub_type: 杂志文章


    authors: Marchand J,Evrard E,Guinand B,Cachot J,Quiniou L,Laroche J

    更新日期:2010-08-01 00:00:00

  • Bioenhancement of cadmium transfer along a multi-level food chain.

    abstract::Previous studies have shown that metal partitioned to a subcellular compartment containing trophically available metal (TAM) is readily available to predators and may be enhanced by increased binding of metal to heat-stable proteins (HSP - e.g., metallothioneins). The aim of the current investigation was to determine ...

    journal_title:Marine environmental research

    pub_type: 杂志文章


    authors: Seebaugh DR,Goto D,Wallace WG

    更新日期:2005-06-01 00:00:00

  • Better red than dead? Potential aposematism in a harpacticoid copepod, Metis holothuriae.

    abstract::The conspicuous, red harpacticoid Metis holothuriae grows to a large size (∼600 μm length) and accounts for 29.51% of the numerical meiofaunal abundance within blooms of the toxic, benthic cyanobacterium Lyngbya majuscula. Despite this, the meiobenthic juvenile trumpeter whiting, Sillago maculata, consume M. holothuri...

    journal_title:Marine environmental research

    pub_type: 杂志文章


    authors: Gilby BL,Burfeind DD,Tibbetts IR

    更新日期:2012-03-01 00:00:00

  • In vivo and in vitro effects of benzothiazole on sheepshead minnow (Cyprinodon variegatus).

    abstract::Benzothiazole, a common chemical associated with tire manufacturing and industrial wastewater, is a principal component of both fresh water and estuarine tire leachate, a neurotoxicant to larval sheepshead minnows (Cyprinodon variegatus) in in vivo estuarine studies. The neurotoxic potential of benzothiazole was inves...

    journal_title:Marine environmental research

    pub_type: 杂志文章


    authors: Evans JJ,Shoemaker CA,Klesius PH

    更新日期:2000-07-01 00:00:00

  • Vitamin E co-treatment reduces Aroclor 1254-induced impairment of reproductive neuroendocrine function in Atlantic croaker.

    abstract::We have shown previously that exposure of Atlantic croaker to a PCB mixture (Aroclor 1254) results in impaired reproductive neuroendocrine function. In addition, we have identified hypothalamic tryptophan hydroxylase (TPH), the rate-limiting enzyme in serotonin (5-hydroxytryptamine) synthesis, as a target of PCB neuro...

    journal_title:Marine environmental research

    pub_type: 杂志文章


    authors: Khan IA,Thomas P

    更新日期:2004-08-01 00:00:00

  • Physiological response and photoacclimation capacity of Caulerpa prolifera (Forsskål) J.V. Lamouroux and Cymodocea nodosa (Ucria) Ascherson meadows in the Mar Menor lagoon (SE Spain).

    abstract::The macroalga Caulerpa prolifera colonized the Mar Menor coastal lagoon after the enlargement of the main inlet in 1972, coexisting now with the previous Cymodocea nodosa meadows. The physiological response and the photoacclimation capacity of both species were studied. For this purpose in vivo chlorophyll a fluoresce...

    journal_title:Marine environmental research

    pub_type: 杂志文章


    authors: García-Sánchez M,Korbee N,Pérez-Ruzafa IM,Marcos C,Domínguez B,Figueroa FL,Pérez-Ruzafa Á

    更新日期:2012-08-01 00:00:00