Abstract：
:Immunohistochemical localization of enzymes is compared directly with localization of enzyme activity with (catalytic) enzyme histochemical methods. The two approaches demonstrate principally different aspects of an enzyme. The immunohistochemical method localizes the enzyme protein whether it is active or not whereas the catalytic method visualizes the functionally active enzyme only. Furthermore, enzyme histochemical methods appear to be highly sensitive in comparison with immunohistochemical methods. Finally, the catalytic methods are simple and last 5-60 min at room temp, whereas immunohistochemical methods take hours at least. It can be concluded that enzyme histochemical methods are the methods of choice to localize enzymes because they detect the functional activity of an enzyme, they are relatively simple and their sensitivity is high.
journal_name
Mar Environ Resjournal_title
Marine environmental researchauthors
Van Noorden CJdoi
10.1016/s0141-1136(02)00169-1keywords:
subject
Has Abstractpub_date
2002-09-01 00:00:00pages
575-7issue
3-5eissn
0141-1136issn
1879-0291pii
S0141-1136(02)00169-1journal_volume
54pub_type
杂志文章abstract：:Identifying and quantifying microplastic in marine samples can be facilitated by removing natural organic matter (NOM). Cellulosic material, like chitin, however, are a type of NOM that is resistant to chemical digestion, and difficult to eliminate from samples. To address this, a two-step digestion method was develop...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2020.105080
更新日期：2020-10-01 00:00:00
abstract：:The objective of this study was to determine the potential for dietary transfer of sediment-associated fluoranthene from tubificid oligochaetes (Monopylephorus rubroniveus) to grass shrimp (Palaemonetes pugio). Grass shrimp, either in the presence or absence of sublethal waterborne concentrations of the metabolic inhi...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2006.06.005
更新日期：2007-03-01 00:00:00
abstract：:Meiofauna can play an important role in the carbon fluxes of soft-bottom coastal habitats. Investigation of their feeding behavior and trophic position remains challenging due to their small size. In this study, we determine and compare the food sources used by nematodes and benthic copepods by using stable isotope co...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2019.05.014
更新日期：2019-08-01 00:00:00
abstract：:The transformation of estuaries by human activities continues to alter the biogeochemical balance of the coastal ocean. The disruption of this balance can negatively impact the provision of goods and services, including fisheries, commerce and transportation, recreation and esthetic enjoyment. Here we examine a link, ...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2014.04.006
更新日期：2014-08-01 00:00:00
abstract：:The amount of macroalgal biomass is an important ecosystem variable. Estimates can be made for a sampled area or values can be extrapolated to represent biomass over a larger region. Typically biomass is scaled-up using the area multiplied by the mean: a non-spatial method. Where algal biomass is patchy or shows gradi...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2020.104906
更新日期：2020-04-01 00:00:00
abstract：:We investigated the long-term effects of contaminants in sediment from Baltimore Harbor, MD, USA, on a burrowing amphipod (Leptocheirus plumulosus) via a full life-cycle laboratory exposure. Contaminants in the Baltimore Harbor sediment included PAHs (10,800 ng/g), PCBs (152 ng/g), and heavy metals (including As, Cd, ...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2006.03.006
更新日期：2006-08-01 00:00:00
abstract：:Biodiverse ecosystems are sometimes inherently resistant to invasion, but environmental change can facilitate invasion by disturbing natural communities and providing resources that are underutilised by native species. In such cases, sufficiently abundant native predators may help to limit invasive population growth. ...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2020.105152
更新日期：2020-12-01 00:00:00
abstract：:The coast of The Netherlands is protected by nourishing sand. Generally, two different techniques are used, beach nourishment and shoreface nourishment. The latter technique supplies sand at a water depth of about 5-8m in the surf zone, and has been used on a regular basis since 1997 with increasing volumes since 2001...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2009.03.003
更新日期：2009-07-01 00:00:00
abstract：:The fin whale (Balaenoptera physalus) is the most abundant and widespread mysticete species in the Mediterranean Sea, found mostly in deep, offshore waters of the western and central portion of the region. In the Mediterranean, this species is known to feed mainly on krill, in contrast to its Atlantic counterpart, whi...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2017.03.009
更新日期：2017-06-01 00:00:00
abstract：:Samples of macrobenthic organisms were obtained at seven stations on a presumed pollution gradient from the head of Vancouver Harbour through to outer Howe Sound. Polychaetes (83 apparent species) and molluscs (43 apparent species) were the most abundant faunal groups numerically (44.8 and 47.9%, respectively). Mollus...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/S0141-1136(03)00064-3
更新日期：2004-02-01 00:00:00
abstract：:Reconstructions suggest a massive decline of nearly 1400 ha of kelp forest in North Western Spain in 2007. In line with global rising temperatures, we hypothesized that Sea Surface Temperature (SST) surpassed a lethal threshold for kelp. We examined whether changes in SST correlated to the proposed decline in kelp for...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2013.06.006
更新日期：2013-09-01 00:00:00
abstract：:Extensive research has shown that the early life stages of marine organisms are sensitive to ocean acidification (OA). Less is known, however, on whether larval settlement and metamorphosis may be affected, or by which mechanisms. These are key processes in the life cycle of most marine benthic organisms, since they m...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2020.104977
更新日期：2020-07-01 00:00:00
abstract：:The Water Framework Directives aims a reduction in concentration of hazardous substances in the marine environment. Consequently, there is a need to distinguish between anthropogenically influenced metal concentrations from natural background levels. To better achieve this goal in the Portuguese coast, dissolved and p...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2012.05.010
更新日期：2012-08-01 00:00:00
abstract：:We cloned the vitellogenin gene from the self-fertilizing fish Rivulus marmoratus, and sequenced 12,326 bp. The number of exons of R. marmoratus and rainbow trout vitellogenin genes were different, and also the splicing junctions are different throughout most of the exons and introns but the amino acid similarity of R...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2004.03.063
更新日期：2004-08-01 00:00:00
abstract：:Metal transport in mollusk extrapallial fluid (EPF) that acts as a "bridge" between soft tissues and shell has surprisingly received little attention until now. Using ultrafiltration and radiotracer techniques we determined silver concentrations and speciation in the EPF of the blue mussel Mytilus edulis after short-t...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2010.09.004
更新日期：2011-02-01 00:00:00
abstract：:Intertidal blue mussels, Mytilus edulis, experience hypoxia reoxygenation during tidal emersion and resubmersion cycles, and this is often suggested to represent a major stress for the animals, especially for their respiratory tissues, the gills. We exposed mussels to experimental short and prolonged anoxia and subseq...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2013.09.007
更新日期：2013-12-01 00:00:00
abstract：:The blue mussel (Mytilus spp.) is widely used as a bioindicator for monitoring of coastal water pollution (mussel watch programs). Herein we provide a review of this study field with emphasis on: the suitability of Mytilus spp. as environmental sentinels; uptake and bioaccumulation patterns of key pollutant classes; t...
journal_title：Marine environmental research
pub_type： 杂志文章,评审
doi：10.1016/j.marenvres.2017.07.024
更新日期：2017-09-01 00:00:00
abstract：:Plasma vitellogenin and related parameters in the killifish Fundulus heteroclitus were measured at selected sites in the Chesapeake Bay. In males, vitellogenin was above the detection limit 14% of the time, and detections did not differ between sites or seasons. Few differences in plasma vitellogenin levels were found...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2009.06.006
更新日期：2009-10-01 00:00:00
abstract：:Mucilaginous aggregates produced by planktonic or benthic algae are considered ecological threats to marine systems. The study evaluated the effects of the spread of benthic mucilaginous aggregates on the structure of coralligenous assemblages. The assemblage and the quality of a site subjected to a benthic mucilage b...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2018.06.011
更新日期：2018-09-01 00:00:00
abstract：:In this study the clam, Tapes philippinarum, a filter-feeding bivalve living in soft bottoms, was used to investigate the water/sediment pollution in Venice Lagoon (Venice, Italy), a heavily urbanised and industrialised area. To this end, clams collected from indigenous populations in a clean farming area (Val Dogà) w...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/s0141-1136(00)00037-4
更新日期：2000-07-01 00:00:00
abstract：:The embryotoxicity of CuO NPs was evaluated in the black sea urchin Arbacia lixula embryos, by using 24-well plates. Fertilized eggs were exposed to five doses of CuO NPs ranging from 0.07 to 20 ppb, until pluteus stage. CuO NPs suspensions in artificial seawater formed agglomerates of 80-200 nm size, and copper uptak...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2015.05.010
更新日期：2015-10-01 00:00:00
abstract：:Coral reefs are responsible for a wide array of ecosystem services including shoreline protection. However, the processes involved in delivering this particular service have not been fully understood. The objective of the present review was to compile the main results in the literature regarding the study of shoreline...
journal_title：Marine environmental research
pub_type： 杂志文章,评审
doi：10.1016/j.marenvres.2017.03.007
更新日期：2017-06-01 00:00:00
abstract：:This study aimed to add light-avoidance as a categorizing technique for the study of mesopelagic acoustic layers. Data recorded along the 20° W parallel from 20° N to Iceland showed three types of mesopelagic layers: the non-avoiding non-migrant deep scattering layer (NMDSL), which dropped its intensity toward the nor...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2019.104842
更新日期：2020-02-01 00:00:00
abstract：:Coral reefs are threatened worldwide by global climate change, manifested in anthropogenic ocean warming and acidification. Despite the importance of coral sexual reproduction for the continuity of coral reefs, our understanding of the extent of the impact of climate change on coral sexual reproduction, particularly o...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2020.105215
更新日期：2021-01-01 00:00:00
abstract：:Persistent organic pollutants (POPs), such as toxic lipophilic organochlorine (OC) compounds, accumulate in the blubber tissue of marine mammals. Toxicological sampling methods most frequently target only the superficial blubber layer. Vertical distribution of these contaminants through the blubber mantle may, however...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2013.11.004
更新日期：2014-03-01 00:00:00
abstract：:To better understand the complexity of the brain-pituitary-gonad axis (BPG) in fish, we developed a biologically based pharmacodynamic model capable of accurately predicting the normal functioning of the BPG axis in salmon. This first-generation model consisted of a set of 13 equations whose formulation was guided by ...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2006.04.022
更新日期：2006-07-01 00:00:00
abstract：:Oysters Crassostrea rhizophorae and Crassostrea gigas were kept for fourteen days at four sites in São José, SC, Brazil, chosen according to a sewage discharge contamination gradient. Enzymatic activities of CAT, GST, G6PDH and GR were evaluated in gills and digestive glands. Higher CAT activity was observed in tissue...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2008.02.057
更新日期：2008-07-01 00:00:00
abstract：:The Quality of Rocky Bottoms index (CFR by its Spanish acronym) is a multimetric method used for the assessment of macroalgae communities in accordance to the European Water Framework Directive. In order to improve the precision and accuracy of the assessments, the index was adjusted using a continuous scoring system....
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2014.06.002
更新日期：2014-12-01 00:00:00
abstract：:The concentrations of 18 polychlorinated biphenyls (PCBs), 23 organochlorine pesticides (OCPs), and 16 polycyclic aromatic hydrocarbons (PAHs) were determined in the blubber and liver of 27 bottlenose dolphins (Tursiops truncatus) stranded along the Canary Islands coasts from 1997 to 2011. DDTs (mean of 60,960 and 445...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2014.03.010
更新日期：2014-09-01 00:00:00
abstract：:Mounting evidence suggests that the P-glycoprotein (pgp) efflux pump may be a modulator of bioavailability and a mode of excretion for xenobiotics. Immunohistochemistry was utilized to examine the distribution and inducibility of a pgp like protein in catfish. Immunoreactivity to the MDR C-219 monoclonal antibody was ...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/s0141-1136(00)00116-1
更新日期：2000-07-01 00:00:00
