Abstract：
:Immunohistochemical localization of enzymes is compared directly with localization of enzyme activity with (catalytic) enzyme histochemical methods. The two approaches demonstrate principally different aspects of an enzyme. The immunohistochemical method localizes the enzyme protein whether it is active or not whereas the catalytic method visualizes the functionally active enzyme only. Furthermore, enzyme histochemical methods appear to be highly sensitive in comparison with immunohistochemical methods. Finally, the catalytic methods are simple and last 5-60 min at room temp, whereas immunohistochemical methods take hours at least. It can be concluded that enzyme histochemical methods are the methods of choice to localize enzymes because they detect the functional activity of an enzyme, they are relatively simple and their sensitivity is high.
journal_name
Mar Environ Resjournal_title
Marine environmental researchauthors
Van Noorden CJdoi
10.1016/s0141-1136(02)00169-1keywords:
subject
Has Abstractpub_date
2002-09-01 00:00:00pages
575-7issue
3-5eissn
0141-1136issn
1879-0291pii
S0141-1136(02)00169-1journal_volume
54pub_type
杂志文章abstract：:A quantitative Histological Health Index (HHI) was applied to Antarctic rock cod (Trematomus bernacchii) using gill, liver, spleen, kidney and gonad to assess the impact of wastewater effluent from Davis Station, East Antarctica. A total of 120 fish were collected from 6 sites in the Prydz Bay region of East Antarctic...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2015.05.011
更新日期：2015-08-01 00:00:00
abstract：:The potential of eel (Anguilla anguilla) as a monitoring species for the Thames Estuary, UK, was examined. Hepatic cytochrome P4501A [7-ethoxyresorufin O-deethylase (EROD) activity] and blood vitellogenin (Western analysis) were investigated as biomarkers of exposure to, respectively, organic contaminants and to conta...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/s0141-1136(00)00060-x
更新日期：2000-07-01 00:00:00
abstract：:The 5900 MW Younggwang nuclear power station on the west coast of Korea discharges warm water affecting coastal ecology [KORDI report (2003). Wide area observation of the impact of the operation of Younggwang nuclear power plant 5 and 6, No. BSPI 319-00-1426-3, KORDI, Seoul, Korea]. Here the spatial and temporal chara...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2005.09.001
更新日期：2006-03-01 00:00:00
abstract：:The aim of this work was to characterize the cholinesterases from gills of Crassostrea rhizophorae in order to use them as biomarkers. Gills were homogenized and then centrifuged (9,000 x g, 4 degrees C, 30 min). S9 and Triton X-100 S9 treated (TX S9) fractions were employed as enzyme source. Km(ap) and Vmax were esti...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/s0141-1136(02)00136-8
更新日期：2002-09-01 00:00:00
abstract：:Trampling by human visitors to rocky shores is a known stressor on macroorganisms. However, the effects of trampling on rocky intertidal biofilm, a complex association of microorganisms of ecological importance in coastal communities, have not been quantified. We evaluated the impact of trampling frequency and intensi...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2012.06.001
更新日期：2012-08-01 00:00:00
abstract：:Since May 29, 2006, a mud volcano in the Brantas Delta of the Sidoarjo district has emitted mud that has inundated nearby villages. Pollution in this area has been implicated in detrimental effects on fish health. In fishes, leukocyte and phagocytic cells play a vital role in body defenses. We report for the first tim...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2014.02.004
更新日期：2014-05-01 00:00:00
abstract：:The effects of sub-lethal concentrations of lindane on behavioral, hematological and histological parameters in Etroplus maculatus were investigated. The LC₅₀ value of lindane for E. maculatus was estimated using the Probit method and was found to be 0.028 mg/L. The red blood cell count (RBC) and the hemoglobin (Hb), ...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2011.10.011
更新日期：2012-05-01 00:00:00
abstract：:The Mondego estuary (Portugal) went through different ecological scenarios over the last decades. An eutrophication process led to a decline in the ecosystem quality. The ensuing restoration plan resulted into a gradual ecological recovery, which was impaired by the occurrence of successive extreme climate events that...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2014.03.004
更新日期：2014-07-01 00:00:00
abstract：:Environmental changes have been associated with natural climatic variability or human activity. Water resources management is, perhaps, the most drastic change observed in the coastal environment. However, external forcings such as the El Niño event have important implications in the global and regional hydrological b...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2020.105135
更新日期：2020-10-01 00:00:00
abstract：:Due to the increasing frequency and severity of the coral bleaching events in the context of global warming, there is an urgent need to improve our understanding of the susceptibility of corals to thermal stresses, particularly at the sub-cellular level. In this context, we examined the modulation of the polyp mitocho...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2014.06.008
更新日期：2014-08-01 00:00:00
abstract：:To study the combined effects of ocean acidification (OA) and warming on the growth and photosynthetic performance of the economically important marine macroalga Gracilariopsis lemaneiformis, thalli were grown under ambient low CO2 (390 μatm, LC) and elevated high CO2 (1000 μatm, HC) conditions with culture temperatur...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2020.105204
更新日期：2021-01-01 00:00:00
abstract：:Presented paper describes the dumped conventional munition catalog that provides up-to-date information about potential hazardous objects lied on the seafloor. Specific aspects of particular types of marine historical ordnance were identified and analyzed, along with a way to catalog objects for their further presenta...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2020.105057
更新日期：2020-10-01 00:00:00
abstract：:Economic development, coastal population growth, and expansion of tourism-related activities along coastlines have been the leading causes of marine debris pollution worldwide. While the problem of marine debris pollution has been increasingly recognized, there has been limited research on its socioeconomic drivers, p...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2020.105042
更新日期：2020-09-01 00:00:00
abstract：:Competition for space is a remarkable ecological force, comparable to predation, producing a strong selective pressure on benthic invertebrates. Some invertebrates, thus, possess antimicrobial compounds to reduce surface bacterial growth. Antimicrobial inhibition is the first step in avoiding being overgrown by other ...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2015.02.001
更新日期：2015-04-01 00:00:00
abstract：:The concentrations of 18 polychlorinated biphenyls (PCBs), 23 organochlorine pesticides (OCPs), and 16 polycyclic aromatic hydrocarbons (PAHs) were determined in the blubber and liver of 27 bottlenose dolphins (Tursiops truncatus) stranded along the Canary Islands coasts from 1997 to 2011. DDTs (mean of 60,960 and 445...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2014.03.010
更新日期：2014-09-01 00:00:00
abstract：:The desiccation tolerance of the intertidal seagrass Zostera japonica has been demonstrated in a number of studies; however, the factors limiting expansion of intertidal seagrass species into subtidal zones remain controversial. We transplanted Z. japonica shoots from the intermediate intertidal zone into the plots wi...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2020.104959
更新日期：2020-05-01 00:00:00
abstract：:Accurate and reliable benthic quality indicators are in great demand following the recent developments and the strict time schedule for implementing the European Water Framework Directive. The Mondego estuary has experienced a progressive deterioration during the 1990s, followed by a partial ecological recovery due to...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2009.04.001
更新日期：2009-07-01 00:00:00
abstract：:Shrimps of the Palaemon genus have been affected for over 15 years by a problem of exoskeletal deformities, particularly in the Gironde estuary (France). Given the large numbers of individuals affected, this study focus on a better description of the phenomenon to estimate its impact on shrimps. This study, on Palaemo...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2009.09.001
更新日期：2010-04-01 00:00:00
abstract：:Eelpouts (Zoarces viviparus) sampled at surveillance stations during the fall of 2007 and spring 2008 in different Danish coastal areas, were studied for biomarkers of polycyclic aromatic hydrocarbons (PAHs) exposure and effects. Two analytical techniques, synchronous fluorescence spectrometry (SFS) and high-performan...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2011.09.005
更新日期：2012-04-01 00:00:00
abstract：:The underlying mechanisms of bacterioplankton community assembly and interspecies interactions during harmful algal blooms remain largely unclear. Using 16S rRNA gene amplicon sequencing, we analyzed the bacterioplankton communities over the continuous course of saxitoxin-producing Gymnodinium catenatum blooms and two...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2020.105010
更新日期：2020-09-01 00:00:00
abstract：:Oil pollution of the oceans has been a problem ever since man began to use fossil fuels. Biodegradation by naturally occurring populations of micro-organisms is a major mechanism for the removal of petroleum from the environment. To examine the effects of crude oil pollution on intertidal bacteria, we repeated the sam...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/s0141-1136(99)00080-x
更新日期：2000-06-01 00:00:00
abstract：:Hydrothermal vent systems are inhabited by dense benthic communities adapted to extreme conditions such as high temperature, hydrogen sulphide (H2S) and elevated fluxes of metals. In the present work, a wide range of trace elements (Ag, Al, As, Ba, Cd, Co, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Sb, Se, V and Zn) were measured in...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2017.10.003
更新日期：2017-12-01 00:00:00
abstract：:The removal of turbidity, particles, phytoplankton and zooplankton in water by crumb rubber filtration was investigated. A substantial reduction was achieved. Of the three variables, filter depth, media size and filtration rate, media size had the most significant influence. Smaller media size favored higher removal e...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2005.06.003
更新日期：2006-05-01 00:00:00
abstract：:We have shown previously that exposure of Atlantic croaker to a PCB mixture (Aroclor 1254) results in impaired reproductive neuroendocrine function. In addition, we have identified hypothalamic tryptophan hydroxylase (TPH), the rate-limiting enzyme in serotonin (5-hydroxytryptamine) synthesis, as a target of PCB neuro...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2004.03.077
更新日期：2004-08-01 00:00:00
abstract：:Marine organisms that occur in urbanised bays can be exposed to low-level chronic pollution that results in sublethal changes to behavior or reproduction. The effects of low levels of copper on the reproductive success of a mobile invertebrate were assessed. Free living flatworms are common predators of bivalves and b...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2007.02.004
更新日期：2007-09-01 00:00:00
abstract：:Isotopic studies of archived fish scales have tremendous potential to develop long-term retrospectives that provide important insights into how humans have altered aquatic ecosystems. However, fish specimens in museum archives and other repositories typically date to time periods when the impacts of industrial societi...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2020.104982
更新日期：2020-09-01 00:00:00
abstract：:Nonylphenol (NP) is a breakdown product of alkylphenol polyethoxylates (APEs), an important class of non-ionic surfactants that are widely used in many detergent formulations and plastic products for industrial and domestic use. A complex microbial degradation pattern, characterized by the formation of several metabol...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/s0141-1136(00)00081-7
更新日期：2000-07-01 00:00:00
abstract：:The estuarine dynamics favoring the coexistence of mangroves, seagrass and corals at small river mouths are often poorly understood. We characterize the tidal, day/night and rainfall-induced short-term dynamics in salinity, pH, dissolved oxygen (DO), chlorophyll a (chl a), total suspended matter (TSM), water transpare...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2012.09.002
更新日期：2012-12-01 00:00:00
abstract：:Laeonereis acuta was seasonally collected in an industrially polluted site (P) and in an unpolluted site (UP) at the Patos Lagoon estuary (southern Brazil). Glutathione S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT) activity (U/mg protein) was determined in five groups of worms from each site. Meta...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2004.03.053
更新日期：2004-08-01 00:00:00
abstract：:We studied the accumulation of polychlorinated biphenyls (PCBs), 1,1,1.trichloro-2,2-bis[p-chlorophenyl]ethane (DDT) and its metabolites, and vitamins A and E in the top levels of the Baltic Sea food web. The investigation focused on the transfer of contaminants and vitamins to the ringed seal (Phoca hispida baltica) ...
journal_title：Marine environmental research
pub_type： 杂志文章
doi：10.1016/j.marenvres.2004.10.007
更新日期：2005-09-01 00:00:00
