Abstract:
:Culture of cassava somatic embryos on media with an altered macro- and micro-nutrient salt concentration affected embryo development and germination capability. In the tests, quarter-, half-, full- or double-strength Murashige and Skoog (MS) media were compared. The maximum number of somatic embryos differentiated from a proliferative nodular embryogenic callus (NEC) on either half- or full-strength MS medium, and the greatest numbers of cotyledonary stage embryos were formed on full-strength MS medium. Developed somatic embryos were then desiccated above a saturated K2SO4 solution for 10 d. After transfer to germination medium, embryos that had developed on half- and full-strength MS medium yielded 8.3 and 8.6 germinants g(-1) NEC tissue, respectively. For this important but often disregarded culture factor, either half- or full-strength MS medium is recommended for both the differentiation and development of cassava somatic embryos that are capable of germination.
journal_name
Ann Botjournal_title
Annals of botanyauthors
Groll J,Mycock DJ,Gray VMdoi
10.1093/aob/mcf095keywords:
subject
Has Abstractpub_date
2002-05-01 00:00:00pages
645-8issue
5eissn
0305-7364issn
1095-8290journal_volume
89pub_type
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