Cholesterol crystal binding of biliary immunoglobulin A: visualization by fluorescence light microscopy.

Abstract:

AIM:To assess potential contributions of biliary IgA for crystal agglomeration into gallstones, we visualized cholesterol crystal binding of biliary IgA. METHODS:Crystal binding biliary proteins were extracted from human gallbladder bile using lectin affinity chromatography.Biliary IgA was isolated from the bound protein fraction by immunoaffinity chromatography. Pure cholesterol monohydrate crystals were incubated with biliary IgA and fluoresceine isothiocyanate (FITC)conjugated anti IgA at 37 degree. Samples were examined under polarizing and fluorescence light microscopy with digital image processing. RESULTS:Binding of biliary IgA to cholesterol monohydrate crystals could be visualized with FITC conjugated anti IgA antibodies. Peak fluorescence occurred at crystal edges and dislocations. Controls without biliary IgA or with biliary IgG showed no significant fluorescence. CONCLUSION:Fluorescence light microscopy provided evidence for cholesterol crystal binding of biliary IgA. Cholesterol crystal binding proteins like IgA might be important mediators of crystal agglomeration and growth of cholesterol gallstones by modifying the evolving crystal structures in vivo.

journal_name

World J Gastroenterol

authors

Lammert F,Südfeld S,Busch N,Matern S

doi

10.3748/wjg.v7.i2.198

keywords:

subject

Has Abstract

pub_date

2001-04-01 00:00:00

pages

198-202

issue

2

eissn

1007-9327

issn

2219-2840

journal_volume

7

pub_type

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