[Biometric evaluation of relative DNA and histone concentration in rabbit spermatozoa following administration of cyproterone acetate. Microcytophotometric studies].

Abstract:

:12 male rabbits were administered cyproterone acetate in a dose of 20 mg/kg every 24 hours for the course of 28 days. Typochemical investigations were then carried out on the relative contents of histone and DNA, using the cytophotometric method. The spermatozoid content of DNA was analyzed using the Feulgen staining method and the relative content of histone proteins by the Alfert and Geschwind staining method. Data obtained were analyzed using ZRA and a Zeiss computer. Results showed a significant decrease in the relative DNA concentration and of histones. These results were compared with reference control data and are discussed. It was thought that in the course of the passage of the spermatozoa through the hormonally prepared epididymis the cyproterone acetate induces changes in the spermatozoan biochemical process, which may have caused their lowered biological value.

journal_name

Ginekol Pol

journal_title

Ginekologia polska

authors

Grabski J

keywords:

subject

Has Abstract

pub_date

1975-09-01 00:00:00

pages

951-8

issue

9

eissn

0017-0011

issn

2543-6767

journal_volume

46

pub_type

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