Abstract:
BACKGROUND AND OBJECTIVE:All-trans retinoic acid (ATRA) induces growth arrest and apoptosis in acute myeloblastic leukemia (AML) cells. Since cellular redox state regulates these events, we were interested in studying whether it has any role in the responsiveness of AML cells to ATRA. DESIGN AND METHODS:Two human AML cell lines, the ATRA-sensitive OU-AML-3, and the ATRA-resistant OU-AML-7, were used as models. Clonogenic cell culture assay, annexin V method, and measurement of mitochondrial membrane potential were used for the determination of cell growth and apoptosis. Peroxide formation was analyzed by flow cytometry, glutathione and g-glutamylcysteine synthetase (g-GCS) activity was determined spectrophotometrically, and the expression of manganese superoxide dismutase (MnSOD) by Western blotting. RESULTS:ATRA inhibited clonogenic cell growth and induced apoptosis particularly in OU-AML-3 cells. The OU-AML-7 cells had a higher basal level of glutathione and g-GCS activity than the OU-AML-3 cells. ATRA enhanced the generation of peroxides after 24h exposure, which was more prominent in the sensitive than the resistant cell line and was not preventable by N-acetyl-L-cysteine. ATRA also increased the activity of g-GCS, which was associated with increased intracellular glutathione in the resistant cell line, while the glutathione level was maintained in the sensitive cell line. During ATRA exposure, MnSOD was induced in the sensitive cell line, but not until after 72 h. Buthionine sulfoximine significantly increased the inhibitory effect of ATRA on colony formation in both cell lines, but only marginally enhanced the effect of ATRA on the induction of apoptosis. INTERPRETATION AND CONCLUSIONS:The balance between oxidative and antioxidative actions of ATRA, as well as the basal redox state of the cells seem to have a definite influence on the responsiveness of AML cells to ATRA.
journal_name
Haematologicajournal_title
Haematologicaauthors
Mäntymaa P,Guttorm T,Siitonen T,Säily M,Savolainen ER,Levonen AL,Kinnula V,Koistinen Pkeywords:
subject
Has Abstractpub_date
2000-03-01 00:00:00pages
238-45issue
3eissn
0390-6078issn
1592-8721journal_volume
85pub_type
杂志文章相关文献
HAEMATOLOGICA文献大全abstract::In the European Intergroup EURO-LB02 trial, children and adolescents with lymphoblastic lymphoma underwent the non-Hodgkin lymphoma Berlin-Frankfurt-Münster protocol without prophylactic cranial radiotherapy. The primary aims of this trial were to test whether replacing prednisone with dexamethasone during induction i...
journal_title:Haematologica
pub_type: 临床试验,杂志文章
doi:10.3324/haematol.2015.139162
更新日期:2017-12-01 00:00:00
abstract::e tested whether polymorphic variations in glutathione S-transferase genes (GSTM1, GSTT1, GSTP1) and interleukin-1 (IL-1beta and IL-1RN) genes confer susceptibility to mucosa-associated lymphoid tissue lymphomas (MALT) in a Chinese population. The rates of GSTM1, GSTP1, IL-1beta and IL-1RN genotypes did not differ bet...
journal_title:Haematologica
pub_type: 信件
doi:
更新日期:2004-08-01 00:00:00
abstract:BACKGROUND:Myelodysplastic syndromes are a heterogeneous group of hematopoietic stem cell disorders with a high propensity to transform into acute myeloid leukemia. Heterozygous missense mutations in IDH1 at position R132 and in IDH2 at positions R140 and R172 have recently been reported in acute myeloid leukemia. Howe...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2010.025494
更新日期:2010-10-01 00:00:00
abstract::Shwachman-Diamond syndrome is a congenital bone marrow failure disorder characterized by debilitating neutropenia. The disease is associated with loss-of-function mutations in the SBDS gene, implicated in ribosome biogenesis, but the cellular and molecular events driving cell specific phenotypes in ribosomopathies rem...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2015.131573
更新日期:2015-10-01 00:00:00
abstract:BACKGROUND:31D8 monoclonal antibody (mAb) has been shown to bind heterogeneously to human neutrophils, identifying subsets of cells which differ in their functional response to chemotactic stimuli. In this study we used 31D8 mAb to determine whether differences in neutrophil subpopulations might explain the long-lastin...
journal_title:Haematologica
pub_type: 杂志文章
doi:
更新日期:1995-03-01 00:00:00
abstract::Circulating stem cells (CSC) are well documented in animals and humans. Though their function in normal conditions remains obscure, autologous CSC seem capable of restoring hemopoiesis after myeloablative treatment. With cell separators CSC may be harvested in adequate number, and collection may be further improved gi...
journal_title:Haematologica
pub_type: 杂志文章,评审
doi:
更新日期:1990-11-01 00:00:00
abstract::The mechanisms of drug resistance in multiple myeloma are poorly understood. Here we show that CD47, an integrin-associated receptor, is significantly upregulated in drug resistant myeloma cells in comparison with parental cells, and that high expression of CD47 detected by immunohistochemistry is associated with shor...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2019.227579
更新日期:2020-12-01 00:00:00
abstract::A significant proportion of hematopoietic stem cell transplants are performed with ABO-mismatched donors. The impact of ABO mismatch on outcome following transplantation remains controversial and there are no published data regarding the impact of ABO mismatch in acute myeloid leukemia patients receiving haploidentica...
journal_title:Haematologica
pub_type: 杂志文章,多中心研究
doi:10.3324/haematol.2016.160804
更新日期:2017-06-01 00:00:00
abstract::Even though alterations in platelet counts are presumed to be detrimental, their impact on the survival of patients has not been studied in large cohorts. The prevalence of thrombocytopenia and thrombocytosis was examined in a large inner city outpatient population of 36,262 individuals aged ≥65 years old. A significa...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2013.101949
更新日期:2014-05-01 00:00:00
abstract::Juvenile myelomonocytic leukemia (JMML) is a myeloproliferative/myelodysplastic disorder associated with mutations in the Ras-Raf-MEK-ERK-signaling pathway. B-Raf plays a central role in this pathway. In 65 screened JMML patients we identified no BRAF mutations and we conclude that this gene is unlikely to play a role...
journal_title:Haematologica
pub_type: 信件
doi:10.3324/haematol.11493
更新日期:2007-11-01 00:00:00
abstract::In patients with low and intermediate risk myelodysplastic syndrome and deletion 5q (del(5q)) treated with lenalidomide, monitoring of cytogenetic response is mandatory, since patients without cytogenetic response have a significantly increased risk of progression. Therefore, we have reviewed cytogenetic data of 302 p...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2010.026658
更新日期:2011-02-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:The aim of this study was to evaluate the tolerability and effectiveness of a non-myeloablative conditioning regimen followed by autologous hematopoietic stem cell infusion for the treatment of severe autoimmune diseases. DESIGN AND METHODS:From 1996 patients with severe autoimmune disease no...
journal_title:Haematologica
pub_type: 杂志文章
doi:
更新日期:2000-11-01 00:00:00
abstract::Acute myeloid leukemia carrying cytoplasmic mutated nucleophosmin (NPMc(+) AML) and blastic plasmacytoid dendritic cell neoplasm have been included as new entities in the 4(th) edition (2008) WHO classification of myeloid neoplasms. These conditions may show clinical and pathological overlapping features (leukemic and...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.13855
更新日期:2009-02-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Transplant-related mortality (TRM) following allogeneic hematopoietic stem cell transplantation (HSCT) has been reported to be related to disease stage, duratiion of disease and type of donor. Furthermore, the outcome of transplants performed in the 1990s appears to be better than that of tran...
journal_title:Haematologica
pub_type: 杂志文章
doi:
更新日期:2004-10-01 00:00:00
abstract::C-Mpl and PRV-1 expression was measured in a cohort of 48 patients with essential thrombocythemia (ET). A retrospective analysis was conducted to asses whether the presence of one or both markers correlates with a higher risk of developing thromboembolic complications. In this cohort, PRV-1 overexpression was associat...
journal_title:Haematologica
pub_type: 信件
doi:
更新日期:2005-06-01 00:00:00
abstract::A probabilistic model was used to compare cryoprecipitate to viral inactivated, commercial fibrinogen concentrate to evaluate with regard to the recipient's risk of exposure to an emergent AIDS-like epidemic. In patients who occasionally need a therapeutic dose of fibrinogen, commercial fibrinogen would be marginally ...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.11072
更新日期:2007-06-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Hereditary hyperferritinemia-cataract syndrome (HHCS) is an autosomal dominant disorder characterized by bilateral cataracts and increased serum and tissue L-ferritin, in the absence of iron overload. The deregulation of ferritin production is caused by heterogeneous mutations in the iron regul...
journal_title:Haematologica
pub_type: 杂志文章
doi:
更新日期:1999-06-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Altered adhesive interaction between bone marrow (BM) stroma and progenitors in chronic myeloid leukemia (CML) may be in part caused by abnormal expression of cell adhesion molecules (CAMs) on malignant progenitor cells. Treatment of CML with interferon-a (IFN-a) re-establishes normal hemopoies...
journal_title:Haematologica
pub_type: 杂志文章
doi:
更新日期:2000-02-01 00:00:00
abstract::Diseases with clonal hematopoiesis such as myelodysplastic syndrome and acute myeloid leukemia have high rates of relapse. Only a small subset of acute myeloid leukemia patients are cured with chemotherapy alone. Relapse in these diseases occurs at least in part due to the failure to eradicate leukemic stem cells or h...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2018.188193
更新日期:2018-08-01 00:00:00
abstract::JAK2 V617F, identified in the majority of patients with myeloproliferative neoplasms, tyrosine phosphorylates SOCS3 and escapes its inhibition. Here, we demonstrate that the JAK2 exon 12 mutants described in a subset of V617F-negative MPN cases, also stabilize tyrosine phosphorylated SOCS3. SOCS3 tyrosine phosphorylat...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2008.002352
更新日期:2009-04-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:With the development and refinement of guidance modalities for percutaneous biopsies, many investigators have reported studies supporting the role of guided core needle biopsy in the diagnosis of mediastinal lymphoma. The aims of this report are to evaluate the efficacy of findings at core need...
journal_title:Haematologica
pub_type: 杂志文章
doi:
更新日期:1999-07-01 00:00:00
abstract:BACKGROUND:In a study of childhood acute lymphoblastic leukemia (CoALL 06-97 study), the in vitro sensitivity of the patients' cells to prednisolone, vincristine and asparaginase was introduced as a new additional risk parameter for treatment stratification. In parallel in vivo treatment response was assessed by determ...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2010.039735
更新日期:2011-06-01 00:00:00
abstract:BACKGROUND:Impaired regulation of hepcidin in response to iron is the cause of genetic hemochromatosis associated with defects of HFE and transferrin receptor 2. However, the role of these proteins in the regulation of hepcidin expression is unclear. DESIGN AND METHODS:Hepcidin expression, SMAD and extracellular signa...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2010.027003
更新日期:2010-11-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:A novel role for shedding of the surface molecule L-selectin has been proposed as an adjunctive phenomenon during cell detachment from marrow stroma or vessel endothelium. We wished to examine whether variations in expression of L-selectin on a lymphoma B cell line were linked to shedding. DES...
journal_title:Haematologica
pub_type: 杂志文章
doi:
更新日期:1999-09-01 00:00:00
abstract::Staining for CD27 and CD201 (endothelial protein C receptor) has been recently suggested as an alternative to stem cell antigen-1 (Sca1) to identify hematopoietic stem cells in inbred mouse strains with low or nil expression of SCA1. However, whether staining for CD27 and CD201 is compatible with low fms-like tyrosine...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2018.212910
更新日期:2020-01-01 00:00:00
abstract::The aim of the study was to define the minimal effective dose (MED) of granulocyte colony-stimulating factor (G-CSF) among five daily doses following chemotherapy for peripheral blood stem cell (PBSC) collection. Twenty-five patients were included in this double-blind dose-finding phase II study conducted according to...
journal_title:Haematologica
pub_type: 杂志文章
doi:
更新日期:2006-04-01 00:00:00
abstract:BACKGROUND:Transplantation from an HLA-matched sibling is the treatment of choice for young patients with acquired severe aplastic anemia. For older patients, the acceptable upper age limit for transplantation as first-line treatment varies. The current analysis, therefore, sought to identify age or ages at transplanta...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2010.026682
更新日期:2010-12-01 00:00:00
abstract::Tyrosine kinase inhibitors (TKIs) are highly effective for the treatment of chronic myeloid leukemia (CML), but very few patients are cured. The major drawbacks regarding TKIs are their low efficacy in eradicating the leukemic stem cells responsible for disease maintenance and relapse upon drug cessation. Herein, we p...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2017.169946
更新日期:2018-03-01 00:00:00
abstract:BACKGROUND:Oral deferiprone was suggested to be more effective than subcutaneous desferrioxamine for removing heart iron. Oral once-daily chelator deferasirox has recently been made commercially available but its long-term efficacy on cardiac iron and function has not yet been established. Our study aimed to compare th...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2009.019042
更新日期:2011-01-01 00:00:00
abstract::Heart disease is the leading cause of mortality and one of the main causes of morbidity in beta-thalassemia. The clinical spectrum of the thalassemia syndrome ranges from the severe, transfusion--dependent thalassemia major and the asymptomatic carrier state. Thalassemia intermedia represents a milder form and is usua...
journal_title:Haematologica
pub_type: 杂志文章,评审
doi:10.3324/haematol.10915
更新日期:2007-05-01 00:00:00