Validation of the use of a commercially available kit for the identification of prostate specific antigen (PSA) in semen stains.

Abstract:

:PSA is currently being used to detect and monitor quantitatively the development of prostate cancer by serum levels of PSA and has also been found to be present in high concentrations in semen. Elegantly simple, sensitive, and reproducible methods have been developed for analysis of the presence of PSA, including the Tandem-E PSA Immunoenzymetric Assay. The most common procedures for the forensic identification of semen have focused on the microscopic detection of sperm, acid phosphatase activity, and immunoelectrophoretic methods for the detection of PSA. Although these methods have been used for many years, there are problems associated with each method. The Tandem-E PSA Immunoenzymetric Assay detected PSA in 100% of the forensic casework fabric samples, 80% of the forensic casework vaginal swabs and 100% of the vasectomized individuals tested. The cut-off value was determined to be 1.77 ng/mL. These results indicate that this method can be used to identify the presence of semen in forensically significant specimens.

journal_name

J Forensic Sci

authors

Simich JP,Morris SL,Klick RL,Rittenhouse-Diakun K

keywords:

subject

Has Abstract

pub_date

1999-11-01 00:00:00

pages

1229-31

issue

6

eissn

0022-1198

issn

1556-4029

journal_volume

44

pub_type

杂志文章
  • Postmortem stability of barbiturates in blood and tissues.

    abstract::The stability of five commonly prescribed barbiturates and thiopental in blood and liver at room temperature and at 4 degrees C was studied. Gas chromatography was used for oxybarbiturate analysis while liquid chromatography was used to quantitate thiopental. In blood and liver, greater than 75% of the drugs were dete...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Levine BS,Blanke RV,Valentour JC

    更新日期:1984-01-01 00:00:00

  • A technique to enhance fingerprinting of mummified fingers.

    abstract::This report details a fingerprinting technique for softening mummified fingers and hands using Metaflow and Restorative, a fluid for rehydration of desiccated tissue. This procedure has proven successful in cases of extreme desiccation and has allowed for good fingerprint detail to be developed. ...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Haglund WD

    更新日期:1988-09-01 00:00:00

  • Elimination of bioweapons agents from forensic samples during extraction of human DNA.

    abstract::Collection of DNA for genetic profiling is a powerful means for the identification of individuals responsible for crimes and terrorist acts. Biologic hazards, such as bacteria, endospores, toxins, and viruses, could contaminate sites of terrorist activities and thus could be present in samples collected for profiling....

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/1556-4029.12561

    authors: Timbers J,Wilkinson D,Hause CC,Smith ML,Zaidi MA,Laframboise D,Wright KE

    更新日期:2014-11-01 00:00:00

  • Drug-facilitated sexual assault involving gamma-hydroxybutyric acid.

    abstract::The first case involving an alleged sexual assault linked to the use of gamma-hydroxybutyric acid (GHB) in Oklahoma is reported. A-48-year-old Caucasian woman taking amitriptyline was known to have voluntarily ingested a sports drink containing a relaxing health product. She purportedly experienced unconsciousness tha...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Stillwell ME

    更新日期:2002-09-01 00:00:00

  • Postmortem evaluation of serum and urine neopterin concentrations.

    abstract::Cellular immune response is accompanied by the release of neopterin. Increased neopterin levels in urine and serum are observed in patients during viral infections, autoimmune diseases, and allograft rejections and certain malignant diseases. We investigated postmortem neopterin concentrations in urine and serum sampl...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Ambach E,Tributsch W,Fuchs D,Reibnegger G,Henn R,Wachter H

    更新日期:1991-07-01 00:00:00

  • The postmortem fate of Pat Gregory: a disinterred Native American.

    abstract::In late June 1990, the Mono County Sheriffs Department in Bridgeport, CA contacted the Physical Anthropology Human Identification Laboratory (PAHIL) at California State University, Chico to seek assistance in the identification of a recently discovered skull. To assist with possible identification, the cranium receive...

    journal_title:Journal of forensic sciences

    pub_type: 历史文章,杂志文章

    doi:

    authors: Murad TA,Murad TD

    更新日期:2000-03-01 00:00:00

  • Method for characterization of adhesion properties of trace explosives in fingerprints and fingerprint simulations.

    abstract::The near inevitable transfer of explosive particulate matter through fingerprints makes it possible to detect concealed explosives through surface sampling. Repeatable and well-characterized fingerprint simulation facilitates quantitative comparison between particulate sampling methods for subsequent detection of trac...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Phares DJ,Holt JK,Smedley GT,Flagan RC

    更新日期:2000-07-01 00:00:00

  • A media violence-inspired juvenile sexual homicide offender 13 years later.

    abstract::No follow-up studies exist on how minors who commit sexual homicides adjust once released back into the community. This is an important question given the extreme nature of their crimes, lack of recidivism data, and the expectation that one-half of them will be freed from prison by mid-life. Likewise, no studies exist...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Myers WC,Eggleston CF,Smoak P

    更新日期:2003-11-01 00:00:00

  • Resolution of some enantiomeric amines of forensic interest by high-performance liquid chromatography.

    abstract::The high-performance liquid chromatographic resolution of the enantiomers of amphetamine, methamphetamine, ephedrine, and pseudoephedrine is described. The sugar isothiocyanate, 2,3,4,6-tetra-O-acetyl-beta-D-glucopyranosyl isothiocyanate (GITC), is used as a chiral derivatizing agent, with chromatographic separations ...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Noggle FT Jr,Clark CR

    更新日期:1986-04-01 00:00:00

  • Autoerotic asphyxial death following television broadcast.

    abstract::How individuals who engage in autoerotic asphyxial activities first become aware of its purported erotic potential is debated. The case of an adult male who died from autoerotic asphyxiation shortly after watching a television show that discussed sexual asphyxiation is reported. A request is made that medical examiner...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: O'Halloran RL,Lovell FW

    更新日期:1988-11-01 00:00:00

  • Integrated DNA and fingerprint analyses in the identification of 60-year-old mummified human remains discovered in an Alaskan glacier.

    abstract::This report describes the identification of a merchant mariner who perished in 1948 when Northwest Airlines Flight 4422, a DC-4 carrying 24 seamen and six crew members crashed into Mount Sanford, Alaska. Fifty-one years later, a human forearm and hand were found close by the wreckage of the plane, prompting identifica...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/j.1556-4029.2010.01356.x

    authors: Loreille OM,Parr RL,McGregor KA,Fitzpatrick CM,Lyon C,Yang DY,Speller CF,Grimm MR,Grimm MJ,Irwin JA,Robinson EM

    更新日期:2010-05-01 00:00:00

  • Evaluation of the accuracy of on-site multi-analyte drug testing devices in the determination of the prevalence of illicit drugs in drivers.

    abstract::A principal goal of this research was to conduct a field evaluation of "on-site" multi-analyte drug testing devices to determine the most accurate, efficient, and cost-effective device available for the purpose of rapidly detecting drivers under the influence of drugs. Four on-site kits were selected and evaluated for...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Buchan BJ,Walsh JM,Leaverton PE

    更新日期:1998-03-01 00:00:00

  • Automated extraction of lysergic acid diethylamide (LSD) and N-demethyl-LSD from blood, serum, plasma, and urine samples using the Zymark RapidTrace with LC/MS/MS confirmation.

    abstract::A forensic procedure for the quantitative confirmation of lysergic acid diethylamide (LSD) and the qualitative confirmation of its metabolite, N-demethyl-LSD, in blood, serum, plasma, and urine samples is presented. The Zymark RapidTrace was used to perform fully automated solid-phase extractions of all specimen types...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: de Kanel J,Vickery WE,Waldner B,Monahan RM,Diamond FX

    更新日期:1998-05-01 00:00:00

  • Air embolism during intercourse in pregnancy.

    abstract::Air embolism may rarely cause sudden death during or shortly after pregnancy. Certain obstetric techniques, douching procedures, and blowing air into the vagina have been associated with this phenomenon. A case of venous air embolism in a pregnant woman occurring during sexual intercourse in a rear entry position is p...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Lifschultz BD,Donoghue ER

    更新日期:1983-10-01 00:00:00

  • Generating a Vertebrae-based Method to Discriminate between Korean and U.S. White Male Casualties from the Korean War.

    abstract::Low recovery rate of intact crania in conflict-related contexts necessitates the use of postcranial elements for ancestry estimation. This study aimed to develop a new method to discriminate between Korean and U.S. White casualties from the Korean War using vertebrae. Maximum body heights of C2 through L5 were measure...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/1556-4029.14074

    authors: Jeong Y,Jeong G,Pergande S,Lee K

    更新日期:2019-11-01 00:00:00

  • Gabapentin-related Deaths: Patterns of Abuse and Postmortem Levels.

    abstract::Gabapentin, a GABA analogue, is a nonopioid prescribed for seizure control and neuralgic pain. Its abuse for recreational purposes has been increasing in recent years as the number of prescriptions also increases. In our series, we review 104 cases of decedents who tested positive for gabapentin in postmortem blood sa...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/1556-4029.14021

    authors: Tharp AM,Hobron K,Wright T

    更新日期:2019-07-01 00:00:00

  • Crossbow injuries.

    abstract::The crossbow is an uncommon source of fatal injury. In Los Angeles County, two crossbow homicides have occurred in the past 20 years. Following the second case, a crossbow was test-fired into a fresh pork thigh, resulting in distinctive wounds. Experimental studies also showed that the vanes of the bolt (arrow) may be...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Rogers C,Dowell S,Choi JH,Sathyavagiswaran L

    更新日期:1990-07-01 00:00:00

  • Liquid/solid extraction on diatomaceous earth for drug analysis in postmortem blood.

    abstract::Absorption extraction on diatomaceous earth was examined and found to be compatible with typical postmortem blood specimens encountered in forensic toxicology. The effects of solvent, solvent volume, eluate flow rate, and pH on drug recovery and extract quality were investigated. It was concluded that the best method ...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Logan BK,Stafford DT

    更新日期:1989-05-01 00:00:00

  • Infrared spectra of U.S. automobile original finishes (1998-2000). IX. identification of bismuth oxychloride and silver/white mica pearlescent pigments using extended range FT-IR spectroscopy, XRF spectrometry, and SEM/EDS analysis.

    abstract::Bismuth oxychloride (BiOCl) was the first viable synthetic pearl pigment developed 50 years ago. It was only used for a limited time period in automotive paint (model years 1998-2000), serving to produce luster for a single Chrysler black metallic color. Identification of this pigment in an unknown automotive paint ca...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/1556-4029.12414

    authors: Suzuki EM

    更新日期:2014-09-01 00:00:00

  • Isopropanol and isopropanol deaths-ten years' experience.

    abstract::A population of persons who were found, at death, to have significant levels of isopropyl alcohol (isopropanol) in their blood is described. The role of isopropanol in causing death is reviewed in 57 fatal cases. The contribution of acetone, the major metabolite of isopropanol, to death is assessed. Results indicate t...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Alexander CB,McBay AJ,Hudson RP

    更新日期:1982-07-01 00:00:00

  • Separation of standard opiates and their analysis in pharmaceutical and illicit preparations by paired-ion reverse-phase high-pressure liquid chromatography.

    abstract::The separation of standard opiates in a mixture and their analysis in clandestine and pharmaceutical preparations were accomplished by PIC on a micro-Bondapak C18 column. The identification of the opiates was based on two parameters: retention times and the ratios of absorbance peaks recorded at 254 and 280 nm. No pri...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Soni SK,Dugar SM

    更新日期:1979-04-01 00:00:00

  • Visualization method for fingerprints on skin by impression on a polyethylene terephthalate (PET) semirigid sheet.

    abstract::Based on the phenomenon that static electricity attracts dust, a polyethylene terephthalate (PET) semirigid sheet coated with printing ink was used to visualize impressions of fingerprints on the skin of living and dead bodies. Compared with visualization methods for perspiration fingerprints, this method recovers bet...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Guo YC,Xing LP

    更新日期:1992-03-01 00:00:00

  • Examination of heterogeneous crossing sequences between toner and rollerball pen strokes by digital microscopy and 3-D laser profilometry.

    abstract::The determination of line crossing sequences between rollerball pens and laser printers presents difficulties that may not be overcome using traditional techniques. This research aimed to study the potential of digital microscopy and 3-D laser profilometry to determine line crossing sequences between a toner and an aq...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/j.1556-4029.2012.02103.x

    authors: Montani I,Mazzella W,Guichard M,Marquis R

    更新日期:2012-07-01 00:00:00

  • Facial tissue depths in children with cleft lip and palate.

    abstract::Cleft lip and palate (CLP) is a craniofacial malformation affecting more than seven million people worldwide that results in defects of the hard palate, teeth, maxilla, nasal spine and floor, and maxillodental asymmetry. CLP facial soft-tissue depth (FSTD) values have never been published. The purpose of this research...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/1556-4029.12645

    authors: Starbuck JM,Ghoneima A,Kula K

    更新日期:2015-03-01 00:00:00

  • Clothing damage analysis and the phenomenon of the false sexual assault.

    abstract::This paper describes three recent false sexual assaults examined at the Victoria Forensic Science Centre laboratory where clothing damage analysis assisted in the resolution of the case. Suspected false reports of sexual assaults are often sensitive cases with little other forensic evidence. Any evidential value that ...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Taupin JM

    更新日期:2000-05-01 00:00:00

  • Preliminary study on the effect of heated surfaces upon bloodstain pattern analysis.

    abstract::Bloodstain pattern analysis (BPA) involves the interpretation of distinct blood patterns found at crime scenes following a violent act. In this paper, we explored for the first time the effects of surface temperatures upon blood impacting a horizontal surface (steel) with its implications in BPA explored. Specific sur...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/1556-4029.12185

    authors: Larkin BA,Banks CE

    更新日期:2013-09-01 00:00:00

  • Age estimation by occlusal tooth wear.

    abstract::The purpose of the present investigation was to test the accuracy of a new scoring system in recording tooth wear for age estimation. The material consisted of dental stone casts of 383 volunteers who had sound premolars and molars with normal occlusion. The degree of occlusal wear for all premolar and molar teeth was...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Kim YK,Kho HS,Lee KH

    更新日期:2000-03-01 00:00:00

  • A fatal case of methotrimeprazine overdose.

    abstract::Fatal ingestion of methotrimeprazine is unusual, and while therapeutic drug levels are established as concentrations between 0.02 to 0.14 mg/L, fatal levels are not. The following describes a case of fatal suicidal ingestion of methotrimeprazine in which the measured concentration of methotrimeprazine in the blood was...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Avis SP,Holzbecher MD

    更新日期:1996-11-01 00:00:00

  • Preservation of human tissue immersed for five years in fresh water of known temperature.

    abstract::Two human bodies were recovered from the waters of the Duluth, Minnesota harbor. Extensive adipocere formation resulted in remarkable preservation of gross anatomic features of internal organs. Total time of immersion could be precisely estimated at five years. Water temperature during those five years could also be a...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Cotton GE,Aufderheide AC,Goldschmidt VG

    更新日期:1987-07-01 00:00:00

  • An atypical gunshot wound from a home-made zip gun--the value of a thorough scene investigation.

    abstract::Zip guns and the atypical gunshot wounds they produce are rare in forensic pathology. Because of this, investigators and forensic pathologists may be unfamiliar with their construction, appearance, and the wounds associated with them. A 43-year-old mechanic, with a history of depression was found dead in a washroom st...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/j.1556-4029.2007.00621.x

    authors: Cunliffe CH,Denton JS

    更新日期:2008-01-01 00:00:00