Abstract：
:The quantity and function of osteoblasts require continuous osteogenic differentiation of bone marrow mesenchymal stem cells. Recent evidence suggests that microRNAs (miRNAs) act as important post-transcriptional regulators in a wide range of biological processes, including osteoblastic differentiation. Quercetin has also been found to prevent bone loss. In this study, we investigated the osteogenesis of quercetin and miR-206 on bone marrow mesenchymal stem cells (BMSCs) and their relationship. We observed quercetin enhanced BMSCs proliferation with a dose-dependent manner in Cell Counting Kit-8 (CCK-8). Alizarin red S staining, alkaline phosphatase (ALP) quantification assay, miR-206 and mRNA levels of osteogenesis marker genes by quantitative real-time PCR (qPCR) were used to analyze osteogenic potential. We observed quercetin significantly elevated bone mineralization and the mRNA expression levels of osteoblast-specific genes including Runt-related transcription factor 2 (Runx2), Osterix (OSX), osteocalcin (OCN), and osteopontin (OPN). Correspondly, Cx43 expression were increased, while miR-206 expression were decreased. In the presence of agomir of miR-206, effects of quercetin on mineralization, alkaline phosphatase activity and osteoblast-specific genes expression were suppressed. Most of all, Cx43 protein level was also blocked while overexpression of miR-206 against quercetin effects. Taken together, these data indicated that quercetin promotes BMSCs proliferation and osteogenic differentiation. The osteogenic effect of quercetin is partly modulated through miR-206/Cx43 pathway.
journal_name
Am J Transl Resjournal_title
American journal of translational researchauthors
Zhang Q,Chang B,Zheng G,Du S,Li Xsubject
Has Abstractpub_date
2020-05-15 00:00:00pages
2062-2070issue
5issn
1943-8141journal_volume
12pub_type
杂志文章abstract：BACKGROUND:Accumulating studies have demonstrated that some long non-coding RNAs (lncRNAs) play critical roles in the pathogenesis of atherosclerosis. We aimed to identify circulation lncRNAs that are potential biomarkers to evaluate coronary atherosclerotic plaque stability. METHODS AND RESULTS:The transcriptomes of ...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2019-06-15 00:00:00
abstract：:Nonalcoholic steatohepatitis (NASH) has similar clinical pathological changes to alcoholic hepatitis. It shows increased incidence and young trend year by year. Polyene phosphatidyl choline (PPC) is widely used in clinic for liver disease treatment. The effect and mechanism of PPC on NASH have not been fully elucidate...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2016-05-15 00:00:00
abstract：:The purpose of the current study was to investigate the differences in knee kinematics and kinetics in patients with semilunar lateral meniscus (SLM) and discoid lateral meniscus (DLM) injuries before and after arthroscopic partial meniscectomy during level walking. Fifteen healthy volunteers (control group), thirteen...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2020-06-15 00:00:00
abstract：BACKGROUND:Atherosclerosis remains a leading cause of cardiology disease worldwide, which vascular smooth muscle cells (VSMCs) proliferation and apoptosis are involved. Increasing evidences have revealed that long non-coding RNAs (lncRNAs) considered to be critical regulatory factors of VSMCs function. However, the mol...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2019-06-15 00:00:00
abstract：BACKGROUND:Infection can induce and aggravate chronic kidney disease (CKD), and the chemotaxis of Th22 cells may aggravate CKD. However, the mechanism underlying group A Streptococcus (GAS) infections in CKD through the chemotaxis of Th22 cells remains unknown. METHODS:The experiment was divided into a normal control ...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2019-12-15 00:00:00
abstract：:The coronavirus disease 2019 (COVID-19) pandemic has spread to almost all countries. The currently reported epidemiological statistics show that age, gender, and type of comorbidities may be high-risk factors for critically ill patients with COVID-19. However, there is no comprehensive analysis of these risk factors. ...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2020-10-15 00:00:00
abstract：:Babaodan capsule (BBD), a traditional Chinese (TCM) formula, has been widely used as an alternative remedy for multiple types of malignancies, clinically. However, the underlying mechanisms behind the efficacy of BBD remain poorly understood, particularly in regard to lung cancer. Herein, we demonstrate that BBD induc...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2019-08-15 00:00:00
abstract：:Focal liver lesions (FLLs) include a variety of benign and malignant tumors, and are usually differential diagnosed by ultrasonography. However, traditional two-dimensional contrast-enhanced ultrasound (2D-CEUS) can evaluate a single slice of a lesion. It cannot fully display perfusion of a lesion and the relationship...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2018-05-15 00:00:00
abstract：:Nonalcoholic steatohepatitis represents a significant and rapidly growing unmet medical need. The development of novel therapies has been hindered in part, by the limitations of existing preclinical models. There is a strong need for physiologically relevant in vivo and in vitro liver fibrosis models that are characte...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2019-03-15 00:00:00
abstract：:MicroRNAs (miRNAs) are small regulatory non-coding RNAs that have been reported to play an important role in the tumorigenesis of many cancers. In addition, miRNAs might serve as new promising biomarkers for diagnosis and prognosis and as effective therapeutic targets for patients with such malignancies. Accordingly, ...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2020-03-15 00:00:00
abstract：:MicroRNA-145 (miR-145) can regulate tumor cell invasion, metastasis, apoptosis, proliferation and stem cell differentiation. However, the molecular mechanisms of miR-145 used to regulate ovarian invasion and metastasis remain to be determined. In this study, Transwell cell migration and wound healing assays were used ...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2017-07-15 00:00:00
abstract：:Angiotesin II (Ang II) plays an important role in cardiac remodeling. Fibroblast growth factor inducible-14 (Fn14) is the smallest member of the tumor necrosis factor superfamily of receptors. Currently, little is known about the functional role of Fn14 in the heart. Chiefly, we observe the up-regulation of extracellu...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2016-12-15 00:00:00
abstract：:The survival kinase Akt and the tumor suppressor LKB1 elicit opposite effects on cell proliferation and tumorigenesis. The present study demonstrates that Akt acts as an upstream kinase of LKB1 to promote the phosphorylation at Ser334 and facilitate its binding to 14-3-3 proteins, resulting in a decreased interaction ...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
abstract：:Background: Pericardial patches are frequently used in vascular surgery to close arteriotomies. The early healing of these patches is mediated by attraction of CD34 and ephrinB2-positive cells. Atorvastatin is a commonly used statin drug that promotes healing of cardiovascular injury. We hypothesized that atorvastatin...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2018-12-15 00:00:00
abstract：:Atorvastatin (ATV) has an important pro-survival role in cardiomyocytes after acute myocardial infarction (AMI). The objectives of this study were to: 1) determine whether ATV could affect autophagy of cardiomyocytes via the AMP-activated protein kinase (AMPK)/mammalian target of rapamycin (mTOR) pathway, and 2) inves...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2016-10-15 00:00:00
abstract：:Aspergillus is widely distributed in the Earth's biosphere. It has strong adaptive capacity, and lives as saprophytic or parasitic life. This study aims to investigate the role of E-cadherin for adhesion of Aspergillus fumigatus blastospores in a human epithelial cell line (A549) and search the correlated molecule in ...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2016-05-15 00:00:00
abstract：:Recent studies have shown abnormal expression levels of cyclooxygenase-2 (COX-2) and miR-21b-3p in cerebral ischemia-reperfusion (I/R) rat models. Decreased COX-2 expression could reduce brain injury and thus could be a target of miR-21b-3p according to the miRNA databases (miRDB) analysis. However, its functions and ...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2019-05-15 00:00:00
abstract：:This study examined the homing capacity of human induced pluripotent stem cell-derived endothelial cells (iPSC-ECs) and their response to chemotactic gradients of stromal derived factor-1α (SDF). We have previously shown that EC derived from murine pluripotent stem cells can home to the ischemic hindlimb of the mouse....
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2013-08-15 00:00:00
abstract：:Heaptocellular carcinoma (HCC) is still a great health problem around the world. Recently, the cetuximab has been implicated to have therapeutic values for HCC. However, cetuximab-resistance has also been synchronously reported pertaining to HCC treatment. This study aimed to evaluate the role of eIF5A2 in cetuximab-t...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2016-11-15 00:00:00
abstract：BACKGROUND:Ulcerative colitis (UC) is a chronic inflammatory intestinal disease, and its morbidity is rising worldwide. Previous study indicated that astragaloside II (AS II), a monomeric compound, was used to treat bowel disease. However, the effects of AS II on UC remains unclear. Thus, this study aimed to investigat...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2019-11-15 00:00:00
abstract：:Urine-derived stem cells (USCs) are autologous stem cells that exhibit self-renewal ability and multi-lineage differentiation potential. These characteristics make USCs an ideal cell source for hepatocellular transplantation. Here, we investigated the biological characteristics of USCs and their potential use for the ...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2020-09-15 00:00:00
abstract：:The objective of this paper was to investigate the suitability of the miniature pig as a large animal model of cochlear implantation (CI). Micro-CT scanning and three-dimensional reconstructions of the inner ear were completed in six animals. Photographs of the procedures and measurements of the inner ear were made. T...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2016-12-15 00:00:00
abstract：BACKGROUND:Cyclosporin A (CsA) and tacrolimus block T cell activation by inhibiting the phosphatase calcineurin and preventing translocation from the cytoplasm to the nucleus of the transcription factor Nuclear Factor of Activated T cells (NFAT). NFAT compose a family of transcription factors that are turned on during ...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2009-01-22 00:00:00
abstract：:Breast cancer, a worldwide disease with increasing incidence, develops from ductal/lobular epithelium. Nipple aspirate fluid (NAF), secreted from the breast ducts and lobules, can be analyzed to assess metabolic activity in breast microenvironment. Premalignant and malignant cell alterations may produce biochemical si...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2010-10-30 00:00:00
abstract：:Acute myocardial ischemia/reperfusion (MIR) injury leads to severe arrhythmias and a high lethality. We aim to determine the effect of heat shock protein A12B (HSPA12B), a newly discovered member of the Hsp70 family, on heart injury parameters following MIR surgery. We used HSPA12B transgenic mice to determine its eff...
journal_title：American journal of translational research
pub_type： 杂志文章,收录出版
doi：
更新日期：2015-12-15 00:00:00
abstract：:Cyclooxygenase-2 expression by malignant tumors, including colonic adenocarcinoma, is associated with increased tumor aggression and poor prognosis. Nuclear factor kappa B is a key regulator of cyclooxygenase-2 and is regulated by two pathways, the 'canonical' and the 'alternative' pathway. The alternative pathway is ...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2015-09-15 00:00:00
abstract：BACKGROUND:Cisplatin is a common used anti-tumor drug in ovarian cancer therapy with potent effect. Studies have reported that autophagy works as a cell-survival process in cancer, chloroquine has been added to various chemotherapeutic drugs. In the current study, we aim to evaluate whether chloroquine can enhance the ...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2017-09-15 00:00:00
abstract：:Clostridium difficile colitis is caused by a cytotoxin produced by the anaerobic bacteria C. difficile on the epithelial cells of the large intestine, particularly C. difficile toxin B (Tcd B). Current C. difficile toxin assays have proven to be insensitive and have thus been ruled out from diagnostic purposes. Theref...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2020-08-15 00:00:00
abstract：BACKGROUND AND OBJECTIVES:Salvianolic acid A (SAA) is a main component derived from Salvia miltiorrhiza and has been revealed to protect against cerebral ischemia/reperfusion injury (CIRI). The present study was designed to evaluate the neuroprotective role of SAA in CIRI and explore its underlying mechanism in vivo an...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2020-07-15 00:00:00
abstract：:A keloid is the process of skin healing, collagen synthesis and metabolism of the loss of normal control in a sustained hyperactive state, resulting in excessive proliferation of collagen fibers. A large-scale genome-wide association study (GWAS) has identified multiple single nucleotide polymorphisms (SNPs) in the 3q...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2018-02-15 00:00:00
