Abstract：
:BACKGROUND Bone neoplasms are common in humans and have high lethality. Recently, great progress has been made in understanding the pathophysiological mechanisms, but little is known about the molecular and genetic networks involved. MATERIAL AND METHODS qRT-PCR assays were conducted to detect the expression levels of lncRNA HULC in various cell lines. MTT assay, Transwell assay, and wound-healing assay were performed to investigate the proliferation speed, invasion ability, and migration ability of each cell line, respectively. Western blot analysis was also done to assess the expression level of EMT-related factors. Statistical analysis was performed using the t test, Kaplan-Meier method, and log-rank test. RESULTS Compared to the human normal bone cell line, we found lncRNA HULC was over-expressed in all 6 bone neoplasm cell lines, and we finally chose HT1080 and Saos-2 cell lines, which possessed the highest lncRNA HULC expression level, for the subsequent studies. We then observed that the expression level of lncRNA HULC was negatively correlated with overall survival rate of bone neoplasm patients, which means that lncRNA HULC has prognostic value in patients with bone neoplasms. Thus, we assessed the influence of lncRNA HULC down-regulation on proliferation, invasion, and migration abilities of bone neoplasm cells, and found a significant decrease in these abilities. Finally, we found that down-regulating lncRNA HULC led to decreased expression of EMT-related factors in bone neoplasm cells. CONCLUSIONS LncRNA HULC can promote the tumorigenesis of bone neoplasms through increasing the proliferation, invasion, and migration abilities and the expression level of EMT-related factors.
journal_name
Med Sci Monitjournal_title
Medical science monitor : international medical journal of experimental and clinical researchauthors
Zhang T,Wan CY,Mei XL,Jia P,Wang MJdoi
10.12659/MSM.910220subject
Has Abstractpub_date
2018-08-18 00:00:00pages
5754-5760eissn
1234-1010issn
1643-3750pii
910220journal_volume
24pub_type
杂志文章abstract：:BACKGROUND This study investigated the correlation of programmed death 1 (PD-1) and T cell immunoglobulin and ITIM domain (TIGIT) with clinicopathological characteristics of renal cell carcinoma (RCC) and explored the biological roles of both proteins in the development, metastasis, and invasion of RCC. MATERIAL AND M...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.910388
更新日期：2018-09-28 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.894368
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章,评审
doi：
更新日期：2007-09-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2000-11-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2009-05-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.889753
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2008-12-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/msm.883261
更新日期：2012-08-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
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更新日期：2020-07-05 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
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更新日期：2018-07-09 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2001-11-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.912624
更新日期：2019-01-11 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
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更新日期：2020-06-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.921700
更新日期：2020-02-18 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
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doi：
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/msm.896313
更新日期：2016-09-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 临床试验,杂志文章
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
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doi：
更新日期：2003-12-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 历史文章,杂志文章,评审
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
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