Abstract:
BACKGROUND:Cutaneous leishmaniasis (CL) is an infectious disease caused by various species of Leishmania and transmitted by several species of sand flies. CL is among the most neglected tropical diseases, and it has represented a major health threat over the past 20 years in Morocco. The main objectives of this study were to identify relevant sand fly species and detect Leishmania infection in the most prevalent species and patient skin samples in Taza, a focus of CL in North-eastern Morocco. METHODOLOGY AND FINDING:A total of 3672 sand flies were collected by CDC miniature light traps. Morphological identification permitted the identification of 13 species, namely 10 Phlebotomus species and 3 Sergentomyia species. P. longicuspis was the most abundant species, comprising 64.08% of the total collected sand flies, followed by P. sergenti (20.1%) and P. perniciosus (8.45%). Using nested-kDNA PCR, seven pools of P. sergenti were positive to Leishmania tropica DNA, whereas 23 pools of P. longicuspis and 4 pools of P. perniciosus tested positive for Leishmania infantum DNA. The rates of P. longicuspis and P. perniciosus Leishmania infection were 2.51% (23/915) and 7.27% (4/55), respectively, whereas the infection prevalence of P. sergenti was 3.24%. We also extracted DNA from lesion smears of 12 patients suspected of CL, among them nine patients were positive with enzymatic digestion of ITS1 by HaeIII revealing two profiles. The most abundant profile, present in eight patients, was identical to L. infantum, whereas L. tropica was found in one patient. The results of RFLP were confirmed by sequencing of the ITS1 DNA region. CONCLUSION:This is the first molecular detection of L. tropica and L. infantum in P. sergenti and P. longicuspis, respectively, in this CL focus. Infection of P. perniciosus by L. infantum was identified for the first time in Morocco. This study also underlined the predominance of L. infantum and its vector in this region, in which L. tropica has been considered the causative agent of CL for more than 20 years.
journal_name
PLoS Negl Trop Disjournal_title
PLoS neglected tropical diseasesauthors
Mhaidi I,El Kacem S,Ait Kbaich M,El Hamouchi A,Sarih M,Akarid K,Lemrani Mdoi
10.1371/journal.pntd.0006315subject
Has Abstractpub_date
2018-03-02 00:00:00pages
e0006315issue
3eissn
1935-2727issn
1935-2735pii
PNTD-D-17-01429journal_volume
12pub_type
杂志文章abstract::In Latin America and the Caribbean, around 195 million people live in poverty, a situation that increases the burden of some infectious diseases. Neglected diseases, in particular, are often restricted to poor, marginalized sections of the population. Tools exist to combat these diseases, making it imperative to work ...
journal_title:PLoS neglected tropical diseases
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journal_title:PLoS neglected tropical diseases
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pub_type: 杂志文章
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pub_type: 杂志文章
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abstract::Diarrheal diseases are a major cause of morbidity and mortality worldwide. In many cases, antibiotic therapy is either ineffective or not recommended due to concerns about emergence of resistance. The pathogenesis of several of the most prevalent infections, including cholera and enteroxigenic Escherichia coli, is dom...
journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
doi:10.1371/journal.pntd.0006266
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abstract::Chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus that can cause fever and chronic arthritis in humans. CHIKV that is generated in mosquito or mammalian cells differs in glycosylation patterns of viral proteins, which may affect its replication and virulence. Herein, we compare replication, pathogenicity,...
journal_title:PLoS neglected tropical diseases
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abstract::Global efforts to address schistosomiasis and soil-transmitted helminthiases (STH) include deworming programs for school-aged children that are made possible by large-scale drug donations. Decisions on these mass drug administration (MDA) programs currently rely on microscopic examination of clinical specimens to dete...
journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章,评审
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pub_type: 杂志文章
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更新日期:2015-06-16 00:00:00
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
doi:10.1371/journal.pntd.0000909
更新日期:2010-12-07 00:00:00
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journal_title:PLoS neglected tropical diseases
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journal_title:PLoS neglected tropical diseases
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pub_type: 杂志文章
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
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更新日期:2018-11-21 00:00:00
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章,评审
doi:10.1371/journal.pntd.0006256
更新日期:2018-04-19 00:00:00
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
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更新日期:2019-04-26 00:00:00
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
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更新日期:2016-04-22 00:00:00
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
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更新日期:2014-10-16 00:00:00
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章,meta分析,评审
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更新日期:2016-05-19 00:00:00
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
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更新日期:2018-09-20 00:00:00
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journal_title:PLoS neglected tropical diseases
pub_type: 杂志文章
doi:10.1371/journal.pntd.0009012
更新日期:2020-12-28 00:00:00