Abstract:
BACKGROUND:The interaction between stromal cell-derived factor 1 (SDF-1) and its receptor CXC chemokine receptor 4 (CXCR4) plays an important role in mesenchymal stem cells (MSCs) migration and engraftment. Statins can increase the survival of MSCs. However, whether statins could enhance MSCs migration and engraftment is still unknown. Therefore, we designed the study to investigate whether atorvastatin (ATV) could enhance CXCR4 expression of MSCs and promote them homing toward the injured myocardium. METHODS AND RESULTS:Expression of CXCR4 was evaluated by flow cytometry and real time PCR. A transwell system was used to assess MSCs migration ability. Recruitment of systematically delivered MSCs to the infarcted heart was evaluated in Sprague-Dawley rats with acute myocardial infarction (AMI). ATV pretreatment enhanced the expression of CXCR4 and stimulated MSCs migration in vitro. However, the effect was largely abolished by CXCR4 neutralizing antibody. In AMI models, we found much more ATV-pretreated MSCs homing toward the infarcted myocardium than non-treated cells and this was accompanied by improved cardiac performance. CONCLUSIONS:ATV increases the migration ability of MSCs and improves cardiac performance due to up-regulated expression of CXCR4. These results suggest that ATV pretreatment of donor MSCs is an effective way to promote cell therapeutic potential for AMI.
journal_name
Am J Transl Resjournal_title
American journal of translational researchauthors
Li N,Yang YJ,Qian HY,Li Q,Zhang Q,Li XD,Dong QT,Xu H,Song L,Zhang Hsubject
Has Abstractpub_date
2015-06-15 00:00:00pages
1058-70issue
6issn
1943-8141journal_volume
7pub_type
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journal_title:American journal of translational research
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journal_title:American journal of translational research
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journal_title:American journal of translational research
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doi:
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更新日期:2019-05-15 00:00:00
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journal_title:American journal of translational research
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journal_title:American journal of translational research
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doi:
更新日期:2017-03-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2014-05-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章,评审
doi:
更新日期:2017-01-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-05-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2021-01-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-09-15 00:00:00