Cationic amino acid based lipids as effective nonviral gene delivery vectors for primary cultured neurons.


:The delivery of specific genes into neurons offers a potent approach for treatment of diseases as well as for the study of neuronal cell biology. Here we investigated the capabilities of cationic amino acid based lipid assemblies to act as nonviral gene delivery vectors in primary cultured neurons. An arginine-based lipid, Arg-C3-Glu2C14, and a lysine-based lipid, Lys-C3-Glu2C14, with two different types of counterion, chloride ion (Cl-) and trifluoroacetic acid (TFA-), were shown to successfully mediate transfection of primary cultured neurons with plasmid DNA encoding green fluorescent protein. Among four types of lipids, we optimized their conditions such as the lipid-to-DNA ratio and the amount of pDNA and conducted a cytotoxicity assay at the same time. Overall, Arg-C3-Glu2C14 with TFA- induced a rate of transfection in primary cultured neurons higher than that of Lys-C3-Glu2C14 using an optimal weight ratio of lipid-to-plasmid DNA of 1. Moreover, it was suggested that Arg-C3-Glu2C14 with TFA- showed the optimized value higher than that of Lipofectamine2000 in experimental conditions. Thus, Arg-C3-Glu2C14 with TFA- is a promising candidate as a reliable transfection reagent for primary cultured neurons with a relatively low cytotoxicity.


ACS Chem Neurosci


Aoshima Y,Hokama R,Sou K,Sarker SR,Iida K,Nakamura H,Inoue T,Takeoka S




Has Abstract


2013-12-18 00:00:00










  • High affinity radiopharmaceuticals based upon lansoprazole for PET imaging of aggregated tau in Alzheimer's disease and progressive supranuclear palsy: synthesis, preclinical evaluation, and lead selection.

    abstract::Abnormally aggregated tau is the hallmark pathology of tauopathy neurodegenerative disorders and is a target for development of both diagnostic tools and therapeutic strategies across the tauopathy disease spectrum. Development of carbon-11- or fluorine-18-labeled radiotracers with appropriate affinity and specificity...

    journal_title:ACS chemical neuroscience

    pub_type: 杂志文章


    authors: Fawaz MV,Brooks AF,Rodnick ME,Carpenter GM,Shao X,Desmond TJ,Sherman P,Quesada CA,Hockley BG,Kilbourn MR,Albin RL,Frey KA,Scott PJ

    更新日期:2014-08-20 00:00:00

  • Brain Targeting by Liposome-Biomolecular Corona Boosts Anticancer Efficacy of Temozolomide in Glioblastoma Cells.

    abstract::Temozolomide (TMZ) is the current first-line chemotherapy for treatment of glioblastoma multiforme (GBM). However, similar to other brain therapeutic compounds, access of TMZ to brain tumors is impaired by the blood-brain barrier (BBB) leading to poor response for GBM patients. To overcome this major hurdle, we have s...

    journal_title:ACS chemical neuroscience

    pub_type: 杂志文章


    authors: Arcella A,Palchetti S,Digiacomo L,Pozzi D,Capriotti AL,Frati L,Oliva MA,Tsaouli G,Rota R,Screpanti I,Mahmoudi M,Caracciolo G

    更新日期:2018-12-19 00:00:00

  • Synthesis, Characterization, and Activity of a Triazine Bridged Antioxidant Small Molecule.

    abstract::Metal-ion misregulation and oxidative stress continue to be components of the continually evolving hypothesis describing the molecular origins of Alzheimer's disease. Therefore, these features are viable targets for synthetic chemists to explore through hybridizations of metal-binding ligands and antioxidant units. To...

    journal_title:ACS chemical neuroscience

    pub_type: 杂志文章


    authors: Gonzalez P,Pota K,Turan LS,da Costa VCP,Akkaraju G,Green KN

    更新日期:2017-11-15 00:00:00

  • Could Probiotics Be Used to Mitigate Neuroinflammation?

    abstract::Neuroinflammatory processes promote a constellation of neurochemical and hormonal changes resulting in profound effects on motivational states (anhedonia), mood (depression and anxiety disorders), and cognitive function (decrements in learning and memory). The enhancement of immunoregulatory processes, such as increas...

    journal_title:ACS chemical neuroscience

    pub_type: 杂志文章,评审


    authors: Frank MG,Fonken LK,Watkins LR,Maier SF,Lowry CA

    更新日期:2019-01-16 00:00:00

  • Predicting Multi-Interfacial Binding Mechanisms of NLRP3 and ASC Pyrin Domains in Inflammasome Activation.

    abstract::NLRP3-PYD inflammasome activates an inflammatory pathway in response to a wide variety of cell damage or infections. Dysregulated NLRP3 inflammatory signaling has many chronic inflammatory and autoimmune disorders. NLRP3 and ASC have a PYD, a superfamily member of the Death Domain, which plays a key role in inflammato...

    journal_title:ACS chemical neuroscience

    pub_type: 杂志文章


    authors: Riaz M,Rehman AU,Shah SA,Rafiq H,Lu S,Qiu Y,Wadood A

    更新日期:2021-01-27 00:00:00

  • Intranasally Administered S100A9 Amyloids Induced Cellular Stress, Amyloid Seeding, and Behavioral Impairment in Aged Mice.

    abstract::Amyloid formation and neuroinflammation are major features of Alzheimer's disease pathology. Proinflammatory mediator S100A9 was shown to act as a link between the amyloid and neuroinflammatory cascades in Alzheimer's disease, leading together with Aβ to plaque formation, neuronal loss and memory impairment. In order ...

    journal_title:ACS chemical neuroscience

    pub_type: 杂志文章


    authors: Iashchishyn IA,Gruden MA,Moskalenko RA,Davydova TV,Wang C,Sewell RDE,Morozova-Roche LA

    更新日期:2018-06-20 00:00:00

  • Synaptic plasticity, a symphony in GEF.

    abstract::Dendritic spines are the postsynaptic sites for the majority of excitatory synapses in the mammalian forebrain. While many spines display great stability, others change shape in a matter of seconds to minutes. These rapid alterations in dendritic spine number and size require tight control of the actin cytoskeleton, t...

    journal_title:ACS chemical neuroscience

    pub_type: 杂志文章


    authors: Kiraly DD,Eipper-Mains JE,Mains RE,Eipper BA

    更新日期:2010-05-19 00:00:00

  • Identification of a Novel Multifunctional Ligand for Simultaneous Inhibition of Amyloid-Beta (Aβ42) and Chelation of Zinc Metal Ion.

    abstract::Zinc binding to β-amyloid structure could promote amyloid-β aggregation, as well as reactive oxygen species (ROS) production, as suggested in many experimental and theoretical studies. Therefore, the introduction of multifunctional drugs capable of chelating zinc metal ion and inhibiting Aβ aggregation is a promising ...

    journal_title:ACS chemical neuroscience

    pub_type: 杂志文章


    authors: Asadbegi M,Shamloo A

    更新日期:2019-11-20 00:00:00

  • Functionalization and Characterization of Magnetic Nanoparticles for the Detection of Ferritin Accumulation in Alzheimer's Disease.

    abstract::Early diagnosis in Alzheimer's disease (AD), prior to the appearance of marked clinical symptoms, is critical to prevent irreversible neuronal damage and neural malfunction that lead to dementia and death. Therefore, there is an urgent need to generate new contrast agents which reveal by a noninvasive method the prese...

    journal_title:ACS chemical neuroscience

    pub_type: 杂志文章


    authors: Fernández T,Martínez-Serrano A,Cussó L,Desco M,Ramos-Gómez M

    更新日期:2018-05-16 00:00:00

  • Discovery of a new class of ionotropic glutamate receptor antagonists by the rational design of (2S,3R)-3-(3-carboxyphenyl)-pyrrolidine-2-carboxylic acid.

    abstract::The kainic acid (KA) receptors belong to the class of glutamate (Glu) receptors in the brain and constitute a promising target for the treatment of neurological and/or psychiatric diseases such as schizophrenia, major depression, and epilepsy. Five KA subtypes have been identified and named GluK1-5. In this article, w...

    journal_title:ACS chemical neuroscience

    pub_type: 杂志文章


    authors: Larsen AM,Venskutonytė R,Valadés EA,Nielsen B,Pickering DS,Bunch L

    更新日期:2011-02-16 00:00:00

  • Bromoalkaloids protect primary cortical neurons from induced oxidative stress.

    abstract::Bromoalkaloids are secondary metabolites with a demonstrated high activity in several therapeutic areas. In this research, we probe the neuroprotective and antioxidant activities of hymenialdisine and hymenin. Both structures were tested in an oxidative stress cellular model, consisting of cortical neurons that are in...

    journal_title:ACS chemical neuroscience

    pub_type: 杂志文章


    authors: Leirós M,Alonso E,Rateb ME,Houssen WE,Ebel R,Jaspars M,Alfonso A,Botana LM

    更新日期:2015-02-18 00:00:00

  • Oligomer Diversity during the Aggregation of the Repeat Region of Tau.

    abstract::The molecular mechanism of protein aggregation is of both fundamental and clinical importance as amyloid aggregates are linked to a number of neurodegenerative disorders. Such protein aggregates include macroscopic insoluble fibrils as well as small soluble oligomeric species. Time-dependent resolution of these specie...

    journal_title:ACS chemical neuroscience

    pub_type: 杂志文章


    authors: Kjaergaard M,Dear AJ,Kundel F,Qamar S,Meisl G,Knowles TPJ,Klenerman D

    更新日期:2018-12-19 00:00:00

  • A Novel Multifunctional Compound Camellikaempferoside B Decreases Aβ Production, Interferes with Aβ Aggregation, and Prohibits Aβ-Mediated Neurotoxicity and Neuroinflammation.

    abstract::Accumulating evidence suggested that soluble oligomeric β-amyloid protein (Aβ) exerts diverse roles in neuronal cell death, neuroinflammation, oxidative stress, and the eventual dementia associated with Alzheimer's disease (AD). Developing an agent with multiple properties may be a reasonable strategy for the treatmen...

    journal_title:ACS chemical neuroscience

    pub_type: 杂志文章


    authors: Yang S,Liu W,Lu S,Tian YZ,Wang WY,Ling TJ,Liu RT

    更新日期:2016-04-20 00:00:00

  • Development of Sulfonamide Photoaffinity Inhibitors for Probing Cellular γ-Secretase.

    abstract::γ-Secretase is a multiprotein complex that catalyzes intramembrane proteolysis associated with Alzheimer's disease and cancer. Here, we have developed potent sulfonamide clickable photoaffinity probes that target γ-secretase in vitro and in cells by incorporating various photoreactive groups and walking the clickable ...

    journal_title:ACS chemical neuroscience

    pub_type: 杂志文章


    authors: Crump CJ,Murrey HE,Ballard TE,Am Ende CW,Wu X,Gertsik N,Johnson DS,Li YM

    更新日期:2016-08-17 00:00:00

  • Discovery of Small Molecule Kappa Opioid Receptor Agonist and Antagonist Chemotypes through a HTS and Hit Refinement Strategy.

    abstract::Herein we present the outcome of a high throughput screening (HTS) campaign-based strategy for the rapid identification and optimization of selective and general chemotypes for both kappa (κ) opioid receptor (KOR) activation and inhibition. In this program, we have developed potent antagonists (IC(50) < 120 nM) or ago...

    journal_title:ACS chemical neuroscience

    pub_type: 杂志文章


    authors: Frankowski KJ,Hedrick MP,Gosalia P,Li K,Shi S,Whipple D,Ghosh P,Prisinzano TE,Schoenen FJ,Su Y,Vasile S,Sergienko E,Gray W,Hariharan S,Milan L,Heynen-Genel S,Mangravita-Novo A,Vicchiarelli M,Smith LH,Streicher JM,

    更新日期:2012-03-21 00:00:00

  • Involvement of protein kinase C in morphine tolerance at spinal levels of rats.

    abstract::The present study was performed to investigate the possible role of protein kinase C (PKC) in morphine tolerance at spinal levels of rats. Intrathecal injection of 10 μg of morphine induced increases in the hindpaw withdrawal latency (HWL) to noxious thermal and mechanical stimulation in rats. After intrathecal inject...

    journal_title:ACS chemical neuroscience

    pub_type: 杂志文章


    authors: Jin WY,Yu LC

    更新日期:2010-02-17 00:00:00

  • Reversal of Peripheral Neuropathic Pain by the Small-Molecule Natural Product Physalin F via Block of CaV2.3 (R-Type) and CaV2.2 (N-Type) Voltage-Gated Calcium Channels.

    abstract::No universally efficacious therapy exists for chronic pain, a disease affecting one-fifth of the global population. An overreliance on the prescription of opioids for chronic pain despite their poor ability to improve function has led to a national opioid crisis. In 2018, the NIH launched a Helping to End Addiction Lo...

    journal_title:ACS chemical neuroscience

    pub_type: 杂志文章


    authors: Shan Z,Cai S,Yu J,Zhang Z,Vallecillo TGM,Serafini MJ,Thomas AM,Pham NYN,Bellampalli SS,Moutal A,Zhou Y,Xu GB,Xu YM,Luo S,Patek M,Streicher JM,Gunatilaka AAL,Khanna R

    更新日期:2019-06-19 00:00:00

  • Inhibition of Serine Palmitoyltransferase by a Small Organic Molecule Promotes Neuronal Survival after Astrocyte Amyloid Beta 1-42 Injury.

    abstract::Alzheimer's disease (AD) is a slow-progressing disease of the brain characterized by symptoms such as impairment of memory and other cognitive functions. AD is associated with an inflammatory process that involves astrocytes and microglial cells, among other components. Astrocytes are the most abundant type of glial c...

    journal_title:ACS chemical neuroscience

    pub_type: 杂志文章


    authors: De Vita T,Albani C,Realini N,Migliore M,Basit A,Ottonello G,Cavalli A

    更新日期:2019-03-20 00:00:00

  • Binding of Metal-Ion-Induced Tau Oligomers to Lipid Surfaces Is Enhanced by GSK-3β-Mediated Phosphorylation.

    abstract::While fibrillar deposits of hyperphosphorylated protein tau are a key hallmark of several neurodegenerative diseases such as Alzheimer's disease, small oligomers have been speculated to be the key toxic aggregate species. Trivalent metal ions were shown to promote tau oligomer formation in vitro. However, little is kn...

    journal_title:ACS chemical neuroscience

    pub_type: 杂志文章


    authors: Nuebling GS,Plesch E,Ruf VC,Högen T,Lorenzl S,Kamp F,Giese A,Levin J

    更新日期:2020-03-18 00:00:00

  • Modulation of Alzheimer's Aβ protofilament-membrane interactions by lipid headgroups.

    abstract::The molecular pathogenesis of Alzheimer's disease (AD) is complex and sparsely understood. The relationship between AD's amyloid β (Aβ) peptides and neuronal membranes is central to Aβ's cytotoxicity and is directly modulated by the composition of the lipid headgroups. Molecular studies of the insertion of model Aβ40 ...

    journal_title:ACS chemical neuroscience

    pub_type: 杂志文章


    authors: Tofoleanu F,Brooks BR,Buchete NV

    更新日期:2015-03-18 00:00:00

  • N-Terminal Acetylation Preserves α-Synuclein from Oligomerization by Blocking Intermolecular Hydrogen Bonds.

    abstract::The abnormal aggregation of α-synuclein (α-Syn) is closely associated with Parkinson's disease. Different post-translational modifications of α-Syn have been identified and contribute distinctly in α-Syn aggregation and cytotoxicity. Recently, α-Syn was reported to be N-terminally acetylated in cells, yet the function...

    journal_title:ACS chemical neuroscience

    pub_type: 杂志文章


    authors: Bu B,Tong X,Li D,Hu Y,He W,Zhao C,Hu R,Li X,Shao Y,Liu C,Zhao Q,Ji B,Diao J

    更新日期:2017-10-18 00:00:00

  • Microscopic visualization of metabotropic glutamate receptors on the surface of living cells using bifunctional magnetic resonance imaging probes.

    abstract::A series of bimodal metabotropic glutamate-receptor targeted MRI contrast agents has been developed and evaluated, based on established competitive metabotropic Glu receptor subtype 5 (mGluR5) antagonists. In order to directly visualize mGluR5 binding of these agents on the surface of live astrocytes, variations in th...

    journal_title:ACS chemical neuroscience

    pub_type: 杂志文章


    authors: Mishra A,Mishra R,Gottschalk S,Pal R,Sim N,Engelmann J,Goldberg M,Parker D

    更新日期:2014-02-19 00:00:00

  • Unraveling the Role of Receptor for Advanced Glycation End Products (RAGE) and Its Ligands in Myasthenia Gravis.

    abstract::Myasthenia gravis (MG) is an autoimmune T cell-dependent B cell-mediated disorder of the neuromuscular junction (NMJ) characterized by fluctuating skeletal muscle weakness, most commonly attributed to pathogenic autoantibodies against postsynaptic nicotinic acetylcholine receptors (AChRs). Although MG pathogenesis is ...

    journal_title:ACS chemical neuroscience

    pub_type: 杂志文章


    authors: Angelopoulou E,Paudel YN,Piperi C

    更新日期:2020-03-04 00:00:00

  • DARK Classics in Chemical Neuroscience: Opium, a Historical Perspective.

    abstract::Opium is the latex from the opium poppy Papaver somniferum L., which humankind has utilized since ancient Mesopotamia all the way to modern times. Opium used to be surrounded in divine mystery or magic-like abilities and was given to cure a wide variety of diseases until its analgesic, antitussive, and antidiarrheal p...

    journal_title:ACS chemical neuroscience

    pub_type: 历史文章,杂志文章,评审


    authors: Presley CC,Lindsley CW

    更新日期:2018-10-17 00:00:00

  • Olive Oil Lignan (+)-Acetoxypinoresinol Peripheral Motor and Neuronal Protection against the Tremorgenic Mycotoxin Penitrem A Toxicity via STAT1 Pathway.

    abstract::Penitrem A, PA, is an indole diterpene alkaloid produced by several fungal species. PA acts as a selective Ca2+-dependent K-channels (Maxi-K, BK) antagonist in brain, causing motor system dysfunctions including tremors and seizures. However, its molecular mechanism at the peripheral nervous system (PNS) is still ambig...

    journal_title:ACS chemical neuroscience

    pub_type: 杂志文章


    authors: Qusa MH,Abdelwahed KS,Meyer SA,El Sayed KA

    更新日期:2020-11-04 00:00:00

  • Analysis and synthesis in olfaction.

    abstract::Natural environments contain numerous volatile compounds emanating from a large number of sources, and the survival of many animals depends on their ability to segregate odors of interest within complex odorous scenes. In a recent paper, we described how the ability of mice to detect odors within mixtures depends on t...

    journal_title:ACS chemical neuroscience

    pub_type: 评论,杂志文章


    authors: Rokni D,Murthy VN

    更新日期:2014-10-15 00:00:00

  • Behavioral and Biochemical Implications of Dendrimeric Rivastigmine in Memory-Deficit and Alzheimer's Induced Rodents.

    abstract::Exploration of dendrimers for effective drug delivery is giving promising results. The present study was designed and performed to explore the dendrimeric (polyamidoamine-lactoferrin; PAMAM-Lf) formulations for the effective rivastigmine (RIV) delivery against the Alzheimer's induced animal model using lactoferrin as ...

    journal_title:ACS chemical neuroscience

    pub_type: 杂志文章


    authors: Gothwal A,Singh H,Jain SK,Dutta A,Borah A,Gupta U

    更新日期:2019-08-21 00:00:00

  • Newly Identified Aplysia SPTR-Gene Family-Derived Peptides: Localization and Function.

    abstract::When individual neurons in a circuit contain multiple neuropeptides, these peptides can target different sets of follower neurons. This endows the circuit with a certain degree of flexibility. Here we identified a novel family of peptides, the Aplysia SPTR-Gene Family-Derived peptides (apSPTR-GF-DPs). We demonstrated ...

    journal_title:ACS chemical neuroscience

    pub_type: 杂志文章


    authors: Zhang G,Yuan WD,Vilim FS,Romanova EV,Yu K,Yin SY,Le ZW,Xue YY,Chen TT,Chen GK,Chen SA,Cropper EC,Sweedler JV,Weiss KR,Jing J

    更新日期:2018-08-15 00:00:00

  • Reversal of aggregation using β-breaker dipeptide containing peptides: Application to Aβ(1-40) self-assembly and its inhibition.

    abstract::Reversion of protein or peptide aggregation is a formidable task, important in various domains of research at the interface of chemistry, medicine, and nanoscience. A novel class of dipeptides, termed as β-breaker dipeptides (BBDPs), is identified, which can be incorporated into the self-recognizing sequences to gener...

    journal_title:ACS chemical neuroscience

    pub_type: 杂志文章


    authors: Nadimpally KC,Paul A,Mandal B

    更新日期:2014-05-21 00:00:00

  • Design and Synthesis of 2,3- trans-Proline Analogues as Ligands for Ionotropic Glutamate Receptors and Excitatory Amino Acid Transporters.

    abstract::Development of pharmacological tools for the ionotropic glutamate receptors (iGluRs) is imperative for the study and understanding of the role and function of these receptors in the central nervous system. We report the synthesis of 18 analogues of (2 S,3 R)-2-carboxy-3-pyrrolidine acetic acid (3a), which explores the...

    journal_title:ACS chemical neuroscience

    pub_type: 杂志文章


    authors: Poulie CBM,Alcaide A,Krell-Jørgensen M,Larsen Y,Astier E,Bjørn-Yoshimoto WE,Yi F,Syrenne JT,Storgaard M,Nielsen B,Frydenvang KA,Jensen AA,Hansen KB,Pickering DS,Bunch L

    更新日期:2019-06-19 00:00:00