Abstract：
BACKGROUND:The fungus C. albicans is among the leading agents causing death in immunocompromised patients. Most hospitals rely on conventional morphological techniques such as the germ-tube assay and the API system for correct identification. This technique is subjective and hence error prone. Recently, more and more molecular techniques for correct identification have been developed. The latest is the LightCycler real-time PCR technique coupled with melting curve analysis. MATERIAL/METHODS:One hundred hospital isolates presumed to be C. albicans from four major Lebanese hospitals were tested using the real-time PCR technique. The results were compared with a germ-tube test. Furthermore, all real-time PCR-positive samples were replica plated on growth media at 30 degrees C and 45 degrees C to differentiate between C. albicans and C. dubliniensis. Finally, all PCR-negative samples were identified using the API 20C AUX yeast identification system. RESULTS:Twenty-four hospital isolates were non-albicans by PCR (p<0.001). Of these samples, 17 were germ tube positive (p<0.001). A further 6 samples showed positive identification by PCR, but were germ tube negative (p=0.015). Three of the 24 C. albicans-negative isolates were misidentified by the API 20C AUX. None of the C. albicans real-time PCR-positive samples failed to grow at 45 degrees C, the C. dubliniensis non-permissive temperature. CONCLUSIONS:Considering the impact of false identification on the general public health through the use of wrong antifungal drug treatment and the emergence of novel drug-resistant strains, hospitals should update their classification methods using molecular techniques.
journal_name
Med Sci Monitjournal_title
Medical science monitor : international medical journal of experimental and clinical researchauthors
Yazbek S,Barada G,Basma R,Mahfouz J,Khalaf RAsubject
Has Abstractpub_date
2007-05-01 00:00:00pages
MT7-12issue
5eissn
1234-1010issn
1643-3750pii
9682journal_volume
13pub_type
杂志文章abstract：:Aggressiveness is an ancestral behavior common to all animal species. Its neurophysiological mechanisms are similar in all vertebrates. Males are generally more aggressive than females. In this review, aggressive behavior in rodents, monkeys, and man and the role of testosterone and brain serotonin levels have been co...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章,评审
doi：
更新日期：2005-04-01 00:00:00
abstract：:BACKGROUND miR-23b overexpression can promote cardiomyocyte apoptosis and reduce cell growth under hypoxic conditions, suggesting that miR-23b acts as a biomarker for ST-elevation myocardial infarction (STEMI). The aim of this study was to investigate the effect of miR-23b on STEMI patients. MATERIAL AND METHODS We en...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/msm.908060
更新日期：2018-03-14 00:00:00
abstract：BACKGROUND:Haemorrhagic hypotension is accompanied by an increase in endogenous opioid system activity, however, its significance is not fully recognised. Therefore, the role of endogenous opioid peptides in the central cardiovascular regulation in haemorrhagic shock was reviewed. CONCLUSIONS:Complex central opioid me...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章,评审
doi：
更新日期：2001-05-01 00:00:00
abstract：:BACKGROUND The aim of this study was to investigate the association between mean platelet volume (MPV), C-reactive protein (CRP), and prognosis in patients with acute ischemic stroke (AIS) following intravenous (IV) thrombolytic treatment. MATERIAL AND METHODS A retrospective clinical study included 129 patients withi...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.906813
更新日期：2018-06-05 00:00:00
abstract：BACKGROUND:The aim of this study was to determine whether there are differences in the 24-hour melatonin secretion profile between patients with major depression (MD) before and after clomipramine treatment, and a control group, and also whether or not there exist statistically significant differences in the melatonin ...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2001-09-01 00:00:00
abstract：BACKGROUND:High-frequency oscillatory ventilation (HFOV) has recently been applied to acute respiratory distress syndrome patients. However, the issue of humidification during HFOV has not been investigated. In a bench study, we evaluated humidification during HFOV for adults to test if adequate humidification was achi...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2010-12-01 00:00:00
abstract：:BACKGROUND Abdominal bleeding is a severe complication of duodenal fistula, but few studies have focused on this problem. The purpose of the present study was to investigate the prevalence of and risk factors for intra-abdominal bleeding in patients with external duodenal fistula. MATERIAL AND METHODS From January 201...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.912651
更新日期：2018-12-21 00:00:00
abstract：BACKGROUND:The enzymatic analysis of mitochondrial respiratory chain (MRC) complexes of skeletal muscle is an important step in the diagnosis of mitochondrial disorders. Because of its lesser turbidity and increased sensitivity, mitochondrial fractionation has been increasingly considered the diagnostic method of choic...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2004-09-01 00:00:00
abstract：:BACKGROUND The protein 4.1 family is a family of cytoskeletal proteins that play an important role in maintaining normal cell morphology and cell adhesion, migration, division, and intercellular signaling. The main aim of this study was to explore the prognostic significance of the protein 4.1 family in breast cancer ...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.914085
更新日期：2019-05-07 00:00:00
abstract：:BACKGROUND To investigate the effect of the BMP/Smad signaling pathway on fracture healing and osteogenic ability in senile osteoporotic fracture on humans and rats. MATERIAL AND METHODS Sixty-two patients and well-matched normal controls were enrolled for clinical observation. A rat model of senile osteoporotic fract...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.905958
更新日期：2018-06-25 00:00:00
abstract：:BACKGROUND In this study we investigated changes in the status of antibiotic use in Tianjin since the implementation of the Antibiotic Stewardship Program (ASP) (2011-2013), as well as existing problems, strategies, and outcomes to promote rational clinical antibiotic use. MATERIAL AND METHODS A quasi-experimental stu...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/msm.899848
更新日期：2017-02-09 00:00:00
abstract：:BACKGROUND Balloon kyphoplasty (KP) has been widely applied in the treatment of elderly patients with osteoporosis vertebral compression fracture (OVCF), but there has been little research on the pain relief effect. Therefore, we performed this study of patients who received KP. The study included a set of fluoroscopy...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/msm.906456
更新日期：2017-12-18 00:00:00
abstract：BACKGROUND:Omeprazole is a proton pump inhibitor of increasingly wide application in the treatment of different gastroenterological diseases. The purpose of the study was to evaluate the effect of omeprazole (O) on the level of plasma malondialdehyde (MDA). MATERIAL AND METHODS:Omeprazole was administered intraperiton...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：BACKGROUND:The eradication of Helicobacter pylori (HP) is dependent on several bacteriologic and geographic variables. Multiple-drug regimens have been tried in order to eradicate HPi; however, the optimal drug has not yet been found. MATERIAL/METHODS:In this single-blind, randomized clinical trial, 245 dyspeptic pati...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：2005-04-01 00:00:00
abstract：:BACKGROUND The purpose of this study was to explore the function and mechanism of peroxisome proliferator activated receptor agonist (PPARγ) in the toll-like receptor 2 (TLR2)/nod-like receptor with pyrin domain containing 3 (NLRP3) inflammatory corpuscle pathway of asthmatic mice. MATERIAL AND METHODS Eighteen female...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.910766
更新日期：2018-12-13 00:00:00
abstract：:BACKGROUND This study aimed to investigate intracoronary nicorandil treatment on the no-reflow phenomenon (NRP) during primary percutaneous coronary intervention (PCI) in patients with acute ST-segment elevation myocardial infarction (STEMI) and to compare nicorandil with sodium nitroprusside. MATERIAL AND METHODS Pati...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章,随机对照试验
doi：10.12659/MSM.906815
更新日期：2018-05-04 00:00:00
abstract：:There are contradictory reports in current world literature regarding incidence of perforated peptic ulcers and male to female ratio in recent years. Old concepts of seasonal periodicity are being questioned. In our report we analyze what is changing with regard to demographical data of patients affected, incidence of...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2000-03-01 00:00:00
abstract：:BACKGROUND Er-Miao-San (EMS) is used in traditional Chinese medicine. This study aimed to investigate the effect of different elution fractions of EMS on acute inflammation induced by carrageenan in the rat paw and the possible mechanisms of action. MATERIAL AND METHODS Different aqueous fractions of EMS added to an A...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.916977
更新日期：2019-10-23 00:00:00
abstract：:BACKGROUND The aim of this study was to evaluate the elution of BisGMA, UDMA, TEGDMA, and HEMA monomers from flowable bulk fill composite resins with different resin matrix compositions, polymerized in 4-mm-thick layers, into 3 elution media. MATERIAL AND METHODS Three bulk-fill (SDR® (SDR), X-tra base (XB) and BEAUTI...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.907390
更新日期：2018-07-07 00:00:00
abstract：:BACKGROUND The hepatotoxicity of Tripterygium wilfordii Hook. f. (TWHF) limits its clinic utilization. Qingluo Tongbi formula (QTF) was formulated based on a basic Chinese medicine theory. Previous studies have confirmed the safety and efficacy of QTF in treating rheumatoid arthritis. Therefore, we considered that TWH...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.920376
更新日期：2020-02-15 00:00:00
abstract：:BACKGROUND Sepsis combined with myocardial injury is an important cause of septic shock and multiple organ failure. However, the molecular mechanism of sepsis-induced myocardial dysfunction has not yet been thoroughly studied. Resveratrol has been an important research topic due its organ-protection function, but the ...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.918369
更新日期：2019-12-06 00:00:00
abstract：BACKGROUND:Acute retinal necrosis (ARN) is characterized by the triad of acute vitritis, peripheral necrotizing retinitis and vasculitis. CASE REPORT:We report a case of 54-year-old woman with bilateral acute retinal necrosis associated with neuroinfection. Her past medical history included renal transplantation, hype...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/msm.881890
更新日期：2011-08-01 00:00:00
abstract：:BACKGROUND Acute cerebral infarction is a major clinical subtype of ischemic stroke that has become a leading cause of death and disability worldwide. Aldehyde dehydrogenase 2 (ALDH2) is an important oxidative enzyme in alcohol metabolism. The polymorphism of ALDH2 Glu504Lys polymorphism modifies the activity of this ...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/msm.905206
更新日期：2017-09-23 00:00:00
abstract：BACKGROUND:With the advent of superb microsurgery techniques and advanced stabilization instruments, recent decades have seen great progress in treating nonunions secondary to traumatic fractures. However, those nonunions that are secondary to primary non-Hodgkin's lymphoma of bone and often related to irradiation stil...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章,评审
doi：10.12659/msm.882035
更新日期：2011-11-01 00:00:00
abstract：:BACKGROUND The correlation between serum concentration of neuron specific enolase (NSE), S100B, and the prognosis of patients with acute spinal cord injury (ASCI) remains controversial. MATERIAL AND METHODS Sixty patients with confirmed diagnosis of ASCI were recruited for this study from February 2015 to January 2017...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.907406
更新日期：2018-06-30 00:00:00
abstract：:BACKGROUND Albuminuria has been associated with cardiovascular events, but whether such an association can be explained by endothelial dysfunction is not fully understood. In this study, we examined the relationship between the urine albumin-to-creatinine ratio (UACR) and biomarkers of endothelial function in patients...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/msm.903660
更新日期：2017-09-15 00:00:00
abstract：:Telomeres are located at each end of eukaryotic chromosomes. Their functional role is genomic stability maintenance. The protective role of telomeres depends on various factors, including number of nucleotides repeats, telomere-binding proteins, and telomerase activity. Organ transplantation is the preferred replaceme...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章,评审
doi：10.12659/msm.899490
更新日期：2017-01-11 00:00:00
abstract：BACKGROUND:Lipolytic enzymes, such as lipase, phospholipase A(2), lipoprotein lipase, and hormone-sensitive adipocyte lipase are probably implicated in the pathogenesis of acute pancreatitis. The increased activity of these enzymes can cause specific changes in fatty acid composition, both to free fatty acid (FFA) and ...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2001-09-01 00:00:00
abstract：BACKGROUND:The role of nitric oxide (NO) in the modulation of basal and insulin-stimulated glucose uptake remains controversial. The aim of this study was to investigate the role of NO released from its donors, S-nitrosoglutathione (GSNO) and S-nitroso-N-acetylpenicillamine (SNAP), on glucose uptake in skeletal muscle ...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
abstract：BACKGROUND:Tuberculosis is a world-wide public health problem which may clinically present in many different ways. Here we report on a patient with presumed serpiginous choroiditis (PSC) found to have latent ocular tuberculosis. CASE REPORT:The clinical history and physical examination, complete blood count, erythrocy...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/msm.881839
更新日期：2011-07-01 00:00:00
