Abstract:
BACKGROUND:The enzymatic analysis of mitochondrial respiratory chain (MRC) complexes of skeletal muscle is an important step in the diagnosis of mitochondrial disorders. Because of its lesser turbidity and increased sensitivity, mitochondrial fractionation has been increasingly considered the diagnostic method of choice compared with the more classical analysis of muscle homogenate. In circumstances in which mitochondria become abnormal in number, size or shape, the process of mitochondrial enrichment made by sequential centrifugation and washing may favor the selection of the most normal mitochondria, eliminating the most abnormal ones. In this situation, the study of muscle homogenate, paradoxically, may better reflect what happens in vivo. CASE REPORT:To exemplify this situation we present a 60-year-old woman with a complete mitochondrial phenotype and a 70% heteroplasmic presence of the mtDNA A3243G mutation in muscle tissue. The respiratory and enzymatic activities from mitochondria-enriched muscle suspension were within normal control limits. In contrast, when muscle homogenate was studied, enzyme activities of complexes I, III, and V were found to be decreased. CONCLUSIONS:Although mitochondria-enriched muscle suspensions are usually more informative than muscle homogenates for studies of MRC, in some situations it may be necessary to study both to uncover the biochemical defect.
journal_name
Med Sci Monitjournal_title
Medical science monitor : international medical journal of experimental and clinical researchauthors
Casademont J,Perea M,López S,Beato A,Miró O,Cardellach Fsubject
Has Abstractpub_date
2004-09-01 00:00:00pages
CS49-53issue
9eissn
1234-1010issn
1643-3750pii
4260journal_volume
10pub_type
杂志文章abstract:BACKGROUND:Hyperglycemia is among the potent factors that may induce or facilitate apoptosis. TNF-Related Apoptosis-Inducing Factor (TRAIL) is known for its apoptotic and immunomodulatory effects that have recently been correlated with diabetes. We examined serum-soluble TRAIL (sTRAIL) and high-sensitivity CRP (hs-CRP)...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.889514
更新日期:2013-08-29 00:00:00
abstract:BACKGROUND:In the last decade there has been increasing interest in cytoskeletal research in relation to important process of cell biology. However, after decades of debate many issues remain unsettled. The aim of our study was to estimate the distribution of tubulin in etoposide-induced HL-60 cells and address the que...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::Osteoporosis is a common skeletal disease characterized by bone loss and subsequent increased risk of fragility fractures. Recent advances in our mechanistic understanding of molecular communications among osteoblasts, osteoclasts, and osteocytes give insight into the important roles of the canonical Wnt/β-catenin pat...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章,评审
doi:10.12659/MSM.912309
更新日期:2018-12-03 00:00:00
abstract:BACKGROUND:Researchers are not in complete agreement over the extent to which specific language functions are subserved by certain brain areas. The purpose of this article was to determine neuroanatomical correlates of aphasia following cerebrovascular accident. MATERIAL/METHODS:The participants included 50 stroke pat...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
更新日期:2003-03-01 00:00:00
abstract:BACKGROUND:Ultrasound (US) is currently the best method for detecting thyroid nodules, especially those less than 1 cm in diameter, known as 'incidentaloma'. The ultimate diagnosis of non-palpable masses is done by fine-needle aspiration biopsy (FNAB), especially sonographically controlled (S-FNAB). Because of the incr...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
更新日期:2003-02-01 00:00:00
abstract:BACKGROUND:L-arginine, the precursor of nitric oxide, may restore nitric oxide levels and bioavailability and influence symptom severity, quality of life (QoL), physical performance, and left ventricular (LV) diastolic abnormalities in patients with chronic systolic heart failure (HF). The aim was to evaluate the effec...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章,多中心研究,随机对照试验
doi:
更新日期:2009-12-01 00:00:00
abstract:BACKGROUND:The prevalence of type 2 diabetes mellitus is rapidly increasing, worldwide and also in Hungary. Timely diagnosis and early treatment could be aided by targeted screening. Recognizing this, the Hungarian Diabetes Association initiated a risk-stratified screening with the involvement of primary care physician...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 临床试验,杂志文章
doi:10.12659/msm.883747
更新日期:2013-01-24 00:00:00
abstract::BACKGROUND This study aimed to compare the predictive role of two-dimensional transthoracic echocardiography (2D-TTE) and three-dimensional transthoracic echocardiography (3D-TTE) on in-hospital all-cause mortality in patients with heart failure and reduced ejection fraction (HFrEF) due to ischemic heart disease (IHD)...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.922129
更新日期:2020-06-07 00:00:00
abstract::BACKGROUND Breast cancer has a high mortality rate and is the most common cancer of women worldwide. Our gene co-expression network analysis identified the genes closely related to the pathological stage of breast cancer. MATERIAL AND METHODS We performed weighted gene co-expression network analysis (WGCNA) from the G...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.919046
更新日期:2019-11-23 00:00:00
abstract::BACKGROUND Sepsis is known to trigger impaired T cell function, which relates to immunosuppression, contributing to refractory infection and high mortality. The mechanisms of T cell recovery remain to be elucidated, and novel and effective therapeutics for sepsis are needed. Ouabain, a small molecule of cardiac glycos...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.906889
更新日期:2018-05-02 00:00:00
abstract:BACKGROUND:The purpose of our study was to investigate both qualitatively and quantitatively the microbial content of probiotic products licensed for medicinal purposes. MATERIAL/METHODS:Microbiological analysis was performed on five different brands of probiotic products that claimed to contain lactobacilli and/or bi...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
更新日期:2004-09-01 00:00:00
abstract::Endogenous morphine is present in the mammalian brain as determined by gas chromatography/mass spectrometry. The criteria essential for satisfying the definition of morphine as a neurotransmitter are examined. The detection of endogenous morphine-like compounds inside brain neurons by immunocytochemistry and the Ca(+)...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章,评审
doi:
更新日期:2005-06-01 00:00:00
abstract::BACKGROUND The present study assessed and compared the diagnostic accuracy of elastography (acoustic radiation force impulse, ARFI) with that of Wisteria floribunda agglutinin-positive Mac-2-binding protein (WFA⁺-M2BP) for estimating the stage of hepatic fibrosis in chronic liver disease patients. MATERIAL AND METHODS...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.916533
更新日期:2019-09-24 00:00:00
abstract::BACKGROUND The purpose of this study was to explore the effects of the Na+/K+ ATPase inhibitor ouabain in regulating osteosarcoma (OS) cell stemness. MATERIAL AND METHODS Western blot, qPCR, sphere-forming analysis, DNA methylation analysis, and Ca²⁺ concentration detection were performed to evaluate the stem-like tra...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.919266
更新日期:2019-12-11 00:00:00
abstract:BACKGROUND:The purpose of our research was to determine diagnosis and outcomes for non-contact lens related Acanthamoeba keratitis in south India. MATERIAL/METHODS:A retrospective review of the medical and microbiology records of culture-positive Acanthamoeba cases presenting between 1987 and 2001 to a tertiary eye ca...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
更新日期:2003-04-01 00:00:00
abstract::BACKGROUND The anti-microbial protein cathelicidin LL-37 plays an important role in the pathogenesis of psoriasis by inducing inflammation. Our previous study showed that the chlamydial plasmid-encoded protein pGP3 forms a stable complex with LL-37 to neutralize its pro-inflammatory activity. Here, we explored whether...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.910472
更新日期:2018-07-25 00:00:00
abstract::BACKGROUND Whether type 2 diabetes mellitus (T2DM) is independently associated with structural heart abnormalities is controversial because of confounders associated with T2DM. This study aimed to investigate echocardiographic features in patients with newly diagnosed T2DM, exploring changes in cardiac structure and f...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.918972
更新日期:2020-01-26 00:00:00
abstract:BACKGROUND:This study was designed to test the hypothesis that antioxidant Vitamin C prevents the impairment of endothelial function during prolonged sitting. MATERIAL AND METHODS:Eleven men (24.2 ± 4.4 yrs) participated in 2 randomized 3-h sitting trials. In the sitting without vitamin C (SIT) and the sitting with vi...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章,随机对照试验
doi:10.12659/MSM.893192
更新日期:2015-04-07 00:00:00
abstract::BACKGROUND This study investigated the effect of occupational stress and circadian clock gene polymorphism on sleep disorder of oil workers in Xinjiang, China. MATERIAL AND METHODS We enrolled 2300 Xinjiang oil workers who had been working for at least 1 year. The Chinese revised version of the Occupational Stress Que...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.924202
更新日期:2020-08-01 00:00:00
abstract::BACKGROUND Calcium overload, inflammation, and apoptosis play important roles in myocardial ischemia-reperfusion injury (MIRI). Gastrodin pretreatment can alleviate MIRI. This study observed sarcoplasmic reticulum calcium transport ATPase (Ca2+-ATPase, SERCA) and calcium phosphate (PLB) protein expression in the ventr...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/msm.896835
更新日期:2016-09-19 00:00:00
abstract::BACKGROUND Pedicle screw fixation is one of the most commonly used methods in spine surgery. We introduce a surgical robot system from China based on 3-dimensional fluoroscopy imaging and compare it with the commonly used O-arm navigation system. We study the differences in accuracy, safety, and clinical effect in aux...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.924770
更新日期:2020-09-12 00:00:00
abstract:BACKGROUND:Retinoblastoma (Rb) is the most common primary malignant intraocular tumour in childhood. The "two hit" theory, formulated by Knudson in 1971 to explain the variegated clinical expression of the disease, led to the discovery of the so called tumour suppressor genes and the identification of the Rb1 as the pr...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章,评审
doi:
更新日期:2008-12-01 00:00:00
abstract::BACKGROUND Ethnic background may affect the prevalence of nasal bone absence and the length of the nasal bone. This study aimed to elucidate the significance of absent or hypoplastic fetal nasal bone in the Chinese Han population and to formulate an optimal management plan for patients age 35 or older in cases of isol...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.924387
更新日期:2020-07-26 00:00:00
abstract:BACKGROUND:Although reactive lymphoid hyperplasia (RLH) can be found in various organs, including the gastrointestinal tract, orbit, lung, and skin, its occurrence in the liver is rare. CASE REPORT:We report the case of a 47-year-old RLH woman who was identified with RLH of the liver during clinical follow- up of prim...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
更新日期:2010-07-01 00:00:00
abstract:BACKGROUND:The finding of sex steroid receptor protein in non classical reproductive tissues suggested the possibility that sex steroids may have a relevance to the immune system. MATERIAL/METHODS:The J111 cells were maintained in RPMI 1640 complete medium at 37 degrees C in 5% CO2 in air. Cells were resuspended at 1x...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
更新日期:2006-07-01 00:00:00
abstract:BACKGROUND:Bacillus Anthraces is a non-motile, rod like, gram-positive and aerobic bacillus that produces central oval-shaped spores and characterized by rough, irregular and often comma-shaped colonies in blood agar. About 95% of human anthrax is cutaneous and 5% respiratory. GI anthrax, a very rare type, has been rep...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
更新日期:2002-09-01 00:00:00
abstract::BACKGROUND The role of multidrug resistance 1 gene (MDR1 or ABCB1) polymorphism G2677T was studied in relation to paroxetine therapeutic efficacy and its side effects, as well as its association with selected demographic and clinical characteristics of patients with depressive disorder. MATERIAL AND METHODS To evaluat...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.907434
更新日期:2018-05-13 00:00:00
abstract::BACKGROUND Liver cancer is one of the most common malignancies around the world and one of the major causes of cancer related mortality. The objective of this study was to evaluate the anticancer effect of the natural compound psilostachyin-A on 5-fluorouracil-resistant human liver carcinoma cells and its effects on a...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.916635
更新日期:2019-09-07 00:00:00
abstract::BACKGROUND The cardiac autonomic nervous system plays an essential role in epicardial ganglionated plexi (GP) regulation of atrial fibrillation onset and progression. To date, the activity of GP and the function of the cardiac autonomic nervous system are not well understood. The aim of this study was to determine alt...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/msm.902621
更新日期:2017-07-27 00:00:00
abstract:BACKGROUND:We used an animal model of experimental varicocele to investigate the effects on the ipsilateral testis during the progression of experimental varicocele in rat. MATERIAL/METHODS:Experimental varicocele was induced by partial ligation of the left renal vein in rats. Rats in each control group were subjected...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
更新日期:2008-06-01 00:00:00