Effects of regenerative radioelectric asymmetric conveyer treatment on human normal and osteoarthritic chondrocytes exposed to IL-1β. A biochemical and morphological study.

Abstract:

PURPOSE:Osteoarthritis (OA) is a degenerative disease characterized by a progressive loss of articular cartilage extracellular matrix and is due to functional impairments occurring in chondrocytes. In previous works, we highlighted that Regenerative Tissue Optimization (TO-RGN) treatment with radioelectric asymmetric conveyer (REAC) technology influenced the gene expression profiles controlling stem cell differentiation and the pluripotency of human skin-derived fibroblasts in vitro. Since interleukin-1 beta signaling has been implicated in the induction and progression of this disease (through metalloproteinase-3 synthesis and nitric oxide production), we investigated whether REAC TO-RGN might influence the biochemical and morphological changes induced by interleukin-1 beta in normal and OA chondrocytes. METHODS:The induction of metalloproteinase-3 and proteoglycan synthesis was evaluated by a solid-phase enzyme-amplified sensitivity immunoassay, and nitric oxide production was evaluated with the Griess method. Ultrastructural features were observed by transmission electron microscopy. RESULTS:REAC TO-RGN treatment decreased nitric oxide and metalloproteinase-3 production in normal and OA chondrocytes, while inducing an increase in proteoglycan synthesis. OA chondrocytes were more affected by REAC TO-RGN treatment than were normal chondrocytes. Ultrastructural changes confirmed that REAC TO-RGN may counteract the negative effects of interleukin-1 beta incubation. CONCLUSION:The results of this in vitro study suggest that REAC TO-RGN treatment may represent a new, promising approach for the management of OA.

journal_name

Clin Interv Aging

authors

Collodel G,Fioravanti A,Pascarelli NA,Lamboglia A,Fontani V,Maioli M,Santaniello S,Pigliaru G,Castagna A,Moretti E,Iacoponi F,Rinaldi S,Ventura C

doi

10.2147/CIA.S42229

subject

Has Abstract

pub_date

2013-01-01 00:00:00

pages

309-16

eissn

1176-9092

issn

1178-1998

pii

cia-8-309

journal_volume

8

pub_type

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