Abstract:
:In the this study we have investigated the threshold plasma concentration of lidocaine for reversal of mechanical 'allodynia' in a neuropathic pain model in the rat, defined the concentration-dependent limits of that reversal and compared the acute reversal during intravenous drug infusion with the persistent relief of allodynia assayed 48 h later. Actions of i.v. lidocaine on ipsilateral and contralateral legs were also assessed. Forty rats were sorted into five groups (n = 7-10) and underwent spinal root (L5-6) ligation to produce allodynia, as quantified by a lower force of von Frey hairs at the plantar hindpaw required to elicit paw withdrawal (PWT, paw withdrawal threshold). During surgery, intravenous catheters were placed for programmed lidocaine infusion and in some animals arterial catheters were also inserted for assaying lidocaine blood levels. PWTs were measured in ipsilateral and contralateral paws before and after ligation and during infusions which, beginning at 5 days after surgery, were conducted every other day to incrementing levels (1.1-9.7 microg/ml plasma). Ligation produced allodynia in ipsilateral paws (PWT = 1.22 +/- 0.42 g (+/-SEM)) and in contralateral paws (PWT = 4.99 +/- 0.61 g), both markedly lower than pre-operative control values for either paw (11.31 +/- 0.41 g). The ipsilateral allodynia was partially, but significantly and permanently reversed (to PWT = 6-8 g) after a lidocaine infusion to 2.1 microg/ml in two separate groups (n = 7, 8). Lower concentrations resulted in elevation of PWT during infusion but no sustained relief. The elevation of PWT during infusion at this threshold level among individual animals was positively correlated with the relief measured 48 h later, but higher lidocaine concentrations infused in subsequent dosings could exact no further sustained relief. The residual PWT level, after reversal by threshold lidocaine and greater, was constant for each individual rat tested over the next 14 days but varied substantially among individuals; some were restored to pre-operative PWTs and some were totally unresponsive to drug. Retrospective analysis revealed a significant and unanticipated correlation between the incidence of low pre-operative PWTs (< 10 g) and a lack of sustained reversal of post-operative allodynia by lidocaine. Contralateral allodynia, despite its acute reversal during infusion to 2.1 microg/ml and higher, was not persistently relieved after infusion of lidocaine to any concentration. Repeated infusions to subthreshold levels (<2 microg/ml) did not provide persisting relief of allodynia on either side, and infusions of saline were impotent. These findings show that experimental allodynia results from multiple factors, only some of which are sensitive to lidocaine treatment, and that prolonged reversal of allodynia is limited in extent and likely influenced by pre-existing factors.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Naumov GN,Wilson SM,MacDonald IC,Schmidt EE,Morris VL,Groom AC,Hoffman RM,Chambers AFsubject
Has Abstractpub_date
1999-06-01 00:00:00pages
1835-42eissn
0021-9533issn
1477-9137journal_volume
112 ( Pt 12)pub_type
杂志文章abstract::To determine if providers experienced in the management of human immunodeficiency virus (HIV) disease preferred different treatment regimens than providers with less experience, we analyzed data from a national survey of primary care providers' preferred regimens for the management of 30 HIV-related medical conditions...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1976-11-01 00:00:00
abstract::Epidermal growth factor receptor (EGFR) regulates the growth and progression of human transitional cell carcinoma (TCC) of the bladder. We have shown that therapy targeting EGFR inhibited the growth of human TCC established orthotopically in nude mice. The purpose of this study was to evaluate whether EGFR-directed th...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-03-01 00:00:00
abstract::Epidermal growth factor receptor (EGFR) regulates the growth and progression of human transitional cell carcinoma (TCC) of the bladder. We have shown that therapy targeting EGFR inhibited the growth of human TCC established orthotopically in nude mice. The purpose of this study was to evaluate whether EGFR-directed th...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-03-01 00:00:00
abstract::Contrary to the prevailing view that the axon lacks the capacity to synthesize proteins, a substantial body of evidence points to the existence of a metabolically active endogenous translational machinery. The machinery appears to be largely localized in the cortical zone of the axon, where, in vertebrate axons, it is...
journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:1978-08-01 00:00:00
abstract::Staphylococcal enterotoxin A (SEA) was labeled by the chloramine-T method with 125I to a specific activity of 68 to 300 muCi per mug of SEA and with 131I to specific activity of 8 to 218 muCi per mug of SEA. SEA was partially damaged and aggregated during the labeling and storage. The damage seemed not to be greatly d...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
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abstract::The burden for dealing with family violence and its related problems is steadily being shifted toward local communities. Together, the Office of Injury Control in the Missouri Department of Health and National Center for Violence Prevention developed a comprehensive, multidisciplinary approach to help local communitie...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1976-12-01 00:00:00
abstract::The burden for dealing with family violence and its related problems is steadily being shifted toward local communities. Together, the Office of Injury Control in the Missouri Department of Health and National Center for Violence Prevention developed a comprehensive, multidisciplinary approach to help local communitie...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1976-12-01 00:00:00
abstract::Omega-3 fatty acids have potential antiatherogenic, antithrombotic, and antiarrhythmic properties, but their role in coronary heart disease remains controversial. To evaluate the association of omega-3 fatty acids in adipose tissue with the risk of myocardial infarction in men, a case-control study was conducted in ei...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-05-01 00:00:00
abstract::Two missense mutations and a nine-nucleotide deletion of the cardiac sodium channel (SCN5A) gene have been shown to cause long QT syndrome (LQTS) in several familial cases. We identified a novel missense mutation (R1623Q) of the SCN5A gene in a Japanese girl with sporadic LQTS. We used polymerase chain reaction, singl...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-04-01 00:00:00
abstract::Calcium/calmodulin-dependent protein kinase II (CaM kinase) activity is inhibited in cultured hippocampal cells following direct application of glutamate. The goal of the present study was to determine if hippocampal regions that undergo delayed cell death following glutamate microinfusion would exhibit changes in CaM...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-05-01 00:00:00
abstract::Calcium/calmodulin-dependent protein kinase II (CaM kinase) activity is inhibited in cultured hippocampal cells following direct application of glutamate. The goal of the present study was to determine if hippocampal regions that undergo delayed cell death following glutamate microinfusion would exhibit changes in CaM...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-05-01 00:00:00
abstract::Two groups of children (6-15 years) from a Schistosoma haematobium endemic area were followed for 9 months after praziquantel treatment. Seventy-three children came from an area of high infection while 67 children came from an area of low infection. Pre-treatment infection prevalence in the high infection area (76.6%)...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-06-01 00:00:00
abstract::In the this study we have investigated the threshold plasma concentration of lidocaine for reversal of mechanical 'allodynia' in a neuropathic pain model in the rat, defined the concentration-dependent limits of that reversal and compared the acute reversal during intravenous drug infusion with the persistent relief o...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-06-01 00:00:00
abstract::The relationship between atheroma lipid composition and serum lipoprotein and oxidation measurements has not been fully explored. To address this question, we studied serum, plasma, and aortic wall specimens from 66 subjects undergoing coronary artery bypass graft surgery. The lipid composition of aortic specimens was...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-07-01 00:00:00
abstract::The relationship between atheroma lipid composition and serum lipoprotein and oxidation measurements has not been fully explored. To address this question, we studied serum, plasma, and aortic wall specimens from 66 subjects undergoing coronary artery bypass graft surgery. The lipid composition of aortic specimens was...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-07-01 00:00:00
abstract:BACKGROUND:Mitomycin C (MC), a DNA cross-linking and alkylating agent, targets guanines in the m5CpG sequence with 2-3-fold preference over guanines in unmethylated CpG. Benzo[a]pyrenediolepoxide (BPDE) and several other aromatic carcinogens form guanine adducts with an identical selectivity for m5CpG, and in certain c...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-07-01 00:00:00
abstract:BACKGROUND:Mitomycin C (MC), a DNA cross-linking and alkylating agent, targets guanines in the m5CpG sequence with 2-3-fold preference over guanines in unmethylated CpG. Benzo[a]pyrenediolepoxide (BPDE) and several other aromatic carcinogens form guanine adducts with an identical selectivity for m5CpG, and in certain c...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-07-01 00:00:00
abstract::Nitric oxide (NO) regulates several functions both in the developing and the adult central nervous systems (CNS). During development, NO is assumed to contribute to the histogenetic differentiation of the CNS especially through the modulation of programmed neuronal death. The embryonal and postnatal changes in the dis...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-08-01 00:00:00
abstract::Nitric oxide (NO) regulates several functions both in the developing and the adult central nervous systems (CNS). During development, NO is assumed to contribute to the histogenetic differentiation of the CNS especially through the modulation of programmed neuronal death. The embryonal and postnatal changes in the dis...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-08-01 00:00:00
abstract::According to previous pharmacokinetic studies the bioavailability of fluorine (F) from sodium monofluorophosphate (MFP) doubles that of sodium fluoride (NaF). This paper reports a study designed to verify whether the vertebral bone mass increasing effect of NaF (30 mg F/day) was comparable to that of MFP (15 mg F/day)...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-08-01 00:00:00
abstract::According to previous pharmacokinetic studies the bioavailability of fluorine (F) from sodium monofluorophosphate (MFP) doubles that of sodium fluoride (NaF). This paper reports a study designed to verify whether the vertebral bone mass increasing effect of NaF (30 mg F/day) was comparable to that of MFP (15 mg F/day)...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-08-01 00:00:00
abstract:OBJECTIVE:To examine the descriptive epidemiology of weapon injuries not directly attributable to combat during armed conflict. DESIGN:Prospective cohort study. SETTING:Nangarhar region of Afghanistan, which experienced effective peace, intense fighting, and then peace over six months in 1996. SUBJECTS:608 people ad...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-09-01 00:00:00
abstract:OBJECTIVE:In order to elucidate the potential role of granulocyte-colony-stimulating factor (G-CSF) during the course of normal pregnancy and preeclampsia, we measured the serum concentrations of G-CSF in both normal pregnant women and preeclamptic patients. METHODS:Sera of 10 nonpregnant women, 34 normal pregnant wom...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-09-01 00:00:00
abstract::Tables 1 and 2 offer a summary of information currently available on the sources, dosages, and proposed health benefits of the supplemental and complementary nutritional therapies that can be suggested as alternatives to hormone replacement therapy. These therapies have the additional benefit of being broadly availabl...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-10-01 00:00:00
abstract::Tables 1 and 2 offer a summary of information currently available on the sources, dosages, and proposed health benefits of the supplemental and complementary nutritional therapies that can be suggested as alternatives to hormone replacement therapy. These therapies have the additional benefit of being broadly availabl...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-10-01 00:00:00
abstract::Tables 1 and 2 offer a summary of information currently available on the sources, dosages, and proposed health benefits of the supplemental and complementary nutritional therapies that can be suggested as alternatives to hormone replacement therapy. These therapies have the additional benefit of being broadly availabl...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-10-01 00:00:00
abstract::Tables 1 and 2 offer a summary of information currently available on the sources, dosages, and proposed health benefits of the supplemental and complementary nutritional therapies that can be suggested as alternatives to hormone replacement therapy. These therapies have the additional benefit of being broadly availabl...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-10-01 00:00:00
abstract::The aim was to compare two thyroglobulin-immunoradiometric assays (Tg-IRMA) in the follow-up of patients with differentiated thyroid carcinoma (DTC) in order to set up interassay correlation, correlation to clinical background, and to determine whether a lower functional sensitivity (kit A: 0.5 ng/mL, kit B: 0.3 ng/mL...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-11-01 00:00:00
abstract::The aim was to compare two thyroglobulin-immunoradiometric assays (Tg-IRMA) in the follow-up of patients with differentiated thyroid carcinoma (DTC) in order to set up interassay correlation, correlation to clinical background, and to determine whether a lower functional sensitivity (kit A: 0.5 ng/mL, kit B: 0.3 ng/mL...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-11-01 00:00:00
abstract::Between 1984 and 1997, a total of 444 children (< 15 years) received an orthotopic liver transplant (OLT) at Saint-Luc University Clinics. Biliary atresia constituted the indication for OLT in 304 cases (68%). Median age (range) at OLT was 2.1 years (0.3-14.5). 177 children (40%) received a whole liver graft, 184 (41%...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-11-01 00:00:00