Abstract:
:Epidermal growth factor receptor (EGFR) regulates the growth and progression of human transitional cell carcinoma (TCC) of the bladder. We have shown that therapy targeting EGFR inhibited the growth of human TCC established orthotopically in nude mice. The purpose of this study was to evaluate whether EGFR-directed therapy affects angiogenesis associated with the growth and metastasis of human TCC. We determined the cytostatic effect and the effect on production of angiogenic factors after in vitro treatment of the human TCC cell line 253J B-V with MAb C225, a chimerized monoclonal anti-EGFR antibody. The 253J B-V cells were implanted orthotopically into athymic nude mice, and established tumors (4 weeks) were treated with i.p. MAb C225. Expression of the angiogenic factors vascular endothelial growth factor (VEGF), interleukin-8 (IL-8), and basic fibroblast growth factor (bFGF) was evaluated by immunohistochemistry and in situ mRNA hybridization analyses and correlated with microvessel density evaluated after immunohistochemical staining with anti-CD31. In vitro treatment with MAb C225 inhibited mRNA and protein production of VEGF, IL-8, and bFGF by 253J B-V cells in a dose-dependent manner. MAb C225 therapy of nude mice with established TCCs growing orthotopically resulted in inhibition of growth and metastasis compared with controls (P <0.0005). VEGF, IL-8, and bFGF expression was significantly lower in treated tumors than in controls. The down-regulation of these angiogenic factors preceded the involution of blood vessels. These studies indicate that therapy with anti-EGFR MAb C225 has a significant antitumor effect mediated, in part, by inhibition of angiogenesis.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Sincock PM,Fitter S,Parton RG,Berndt MC,Gamble JR,Ashman LKsubject
Has Abstractpub_date
1999-03-01 00:00:00pages
833-44eissn
0021-9533issn
1477-9137journal_volume
112 ( Pt 6)pub_type
杂志文章abstract::To determine if providers experienced in the management of human immunodeficiency virus (HIV) disease preferred different treatment regimens than providers with less experience, we analyzed data from a national survey of primary care providers' preferred regimens for the management of 30 HIV-related medical conditions...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1976-11-01 00:00:00
abstract::Epidermal growth factor receptor (EGFR) regulates the growth and progression of human transitional cell carcinoma (TCC) of the bladder. We have shown that therapy targeting EGFR inhibited the growth of human TCC established orthotopically in nude mice. The purpose of this study was to evaluate whether EGFR-directed th...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-03-01 00:00:00
abstract::Contrary to the prevailing view that the axon lacks the capacity to synthesize proteins, a substantial body of evidence points to the existence of a metabolically active endogenous translational machinery. The machinery appears to be largely localized in the cortical zone of the axon, where, in vertebrate axons, it is...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1978-08-01 00:00:00
abstract::Staphylococcal enterotoxin A (SEA) was labeled by the chloramine-T method with 125I to a specific activity of 68 to 300 muCi per mug of SEA and with 131I to specific activity of 8 to 218 muCi per mug of SEA. SEA was partially damaged and aggregated during the labeling and storage. The damage seemed not to be greatly d...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-04-01 00:00:00
abstract::The burden for dealing with family violence and its related problems is steadily being shifted toward local communities. Together, the Office of Injury Control in the Missouri Department of Health and National Center for Violence Prevention developed a comprehensive, multidisciplinary approach to help local communitie...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1976-12-01 00:00:00
abstract::The burden for dealing with family violence and its related problems is steadily being shifted toward local communities. Together, the Office of Injury Control in the Missouri Department of Health and National Center for Violence Prevention developed a comprehensive, multidisciplinary approach to help local communitie...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1976-12-01 00:00:00
abstract::Omega-3 fatty acids have potential antiatherogenic, antithrombotic, and antiarrhythmic properties, but their role in coronary heart disease remains controversial. To evaluate the association of omega-3 fatty acids in adipose tissue with the risk of myocardial infarction in men, a case-control study was conducted in ei...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-05-01 00:00:00
abstract::Two missense mutations and a nine-nucleotide deletion of the cardiac sodium channel (SCN5A) gene have been shown to cause long QT syndrome (LQTS) in several familial cases. We identified a novel missense mutation (R1623Q) of the SCN5A gene in a Japanese girl with sporadic LQTS. We used polymerase chain reaction, singl...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-04-01 00:00:00
abstract::Calcium/calmodulin-dependent protein kinase II (CaM kinase) activity is inhibited in cultured hippocampal cells following direct application of glutamate. The goal of the present study was to determine if hippocampal regions that undergo delayed cell death following glutamate microinfusion would exhibit changes in CaM...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-05-01 00:00:00
abstract::Calcium/calmodulin-dependent protein kinase II (CaM kinase) activity is inhibited in cultured hippocampal cells following direct application of glutamate. The goal of the present study was to determine if hippocampal regions that undergo delayed cell death following glutamate microinfusion would exhibit changes in CaM...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-05-01 00:00:00
abstract::Two groups of children (6-15 years) from a Schistosoma haematobium endemic area were followed for 9 months after praziquantel treatment. Seventy-three children came from an area of high infection while 67 children came from an area of low infection. Pre-treatment infection prevalence in the high infection area (76.6%)...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-06-01 00:00:00
abstract::In the this study we have investigated the threshold plasma concentration of lidocaine for reversal of mechanical 'allodynia' in a neuropathic pain model in the rat, defined the concentration-dependent limits of that reversal and compared the acute reversal during intravenous drug infusion with the persistent relief o...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-06-01 00:00:00
abstract::In the this study we have investigated the threshold plasma concentration of lidocaine for reversal of mechanical 'allodynia' in a neuropathic pain model in the rat, defined the concentration-dependent limits of that reversal and compared the acute reversal during intravenous drug infusion with the persistent relief o...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-06-01 00:00:00
abstract::The relationship between atheroma lipid composition and serum lipoprotein and oxidation measurements has not been fully explored. To address this question, we studied serum, plasma, and aortic wall specimens from 66 subjects undergoing coronary artery bypass graft surgery. The lipid composition of aortic specimens was...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-07-01 00:00:00
abstract::The relationship between atheroma lipid composition and serum lipoprotein and oxidation measurements has not been fully explored. To address this question, we studied serum, plasma, and aortic wall specimens from 66 subjects undergoing coronary artery bypass graft surgery. The lipid composition of aortic specimens was...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-07-01 00:00:00
abstract:BACKGROUND:Mitomycin C (MC), a DNA cross-linking and alkylating agent, targets guanines in the m5CpG sequence with 2-3-fold preference over guanines in unmethylated CpG. Benzo[a]pyrenediolepoxide (BPDE) and several other aromatic carcinogens form guanine adducts with an identical selectivity for m5CpG, and in certain c...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-07-01 00:00:00
abstract:BACKGROUND:Mitomycin C (MC), a DNA cross-linking and alkylating agent, targets guanines in the m5CpG sequence with 2-3-fold preference over guanines in unmethylated CpG. Benzo[a]pyrenediolepoxide (BPDE) and several other aromatic carcinogens form guanine adducts with an identical selectivity for m5CpG, and in certain c...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-07-01 00:00:00
abstract::Nitric oxide (NO) regulates several functions both in the developing and the adult central nervous systems (CNS). During development, NO is assumed to contribute to the histogenetic differentiation of the CNS especially through the modulation of programmed neuronal death. The embryonal and postnatal changes in the dis...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-08-01 00:00:00
abstract::Nitric oxide (NO) regulates several functions both in the developing and the adult central nervous systems (CNS). During development, NO is assumed to contribute to the histogenetic differentiation of the CNS especially through the modulation of programmed neuronal death. The embryonal and postnatal changes in the dis...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-08-01 00:00:00
abstract::According to previous pharmacokinetic studies the bioavailability of fluorine (F) from sodium monofluorophosphate (MFP) doubles that of sodium fluoride (NaF). This paper reports a study designed to verify whether the vertebral bone mass increasing effect of NaF (30 mg F/day) was comparable to that of MFP (15 mg F/day)...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-08-01 00:00:00
abstract::According to previous pharmacokinetic studies the bioavailability of fluorine (F) from sodium monofluorophosphate (MFP) doubles that of sodium fluoride (NaF). This paper reports a study designed to verify whether the vertebral bone mass increasing effect of NaF (30 mg F/day) was comparable to that of MFP (15 mg F/day)...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-08-01 00:00:00
abstract:OBJECTIVE:To examine the descriptive epidemiology of weapon injuries not directly attributable to combat during armed conflict. DESIGN:Prospective cohort study. SETTING:Nangarhar region of Afghanistan, which experienced effective peace, intense fighting, and then peace over six months in 1996. SUBJECTS:608 people ad...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-09-01 00:00:00
abstract:OBJECTIVE:In order to elucidate the potential role of granulocyte-colony-stimulating factor (G-CSF) during the course of normal pregnancy and preeclampsia, we measured the serum concentrations of G-CSF in both normal pregnant women and preeclamptic patients. METHODS:Sera of 10 nonpregnant women, 34 normal pregnant wom...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-09-01 00:00:00
abstract::Tables 1 and 2 offer a summary of information currently available on the sources, dosages, and proposed health benefits of the supplemental and complementary nutritional therapies that can be suggested as alternatives to hormone replacement therapy. These therapies have the additional benefit of being broadly availabl...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-10-01 00:00:00
abstract::Tables 1 and 2 offer a summary of information currently available on the sources, dosages, and proposed health benefits of the supplemental and complementary nutritional therapies that can be suggested as alternatives to hormone replacement therapy. These therapies have the additional benefit of being broadly availabl...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-10-01 00:00:00
abstract::Tables 1 and 2 offer a summary of information currently available on the sources, dosages, and proposed health benefits of the supplemental and complementary nutritional therapies that can be suggested as alternatives to hormone replacement therapy. These therapies have the additional benefit of being broadly availabl...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-10-01 00:00:00
abstract::Tables 1 and 2 offer a summary of information currently available on the sources, dosages, and proposed health benefits of the supplemental and complementary nutritional therapies that can be suggested as alternatives to hormone replacement therapy. These therapies have the additional benefit of being broadly availabl...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-10-01 00:00:00
abstract::The aim was to compare two thyroglobulin-immunoradiometric assays (Tg-IRMA) in the follow-up of patients with differentiated thyroid carcinoma (DTC) in order to set up interassay correlation, correlation to clinical background, and to determine whether a lower functional sensitivity (kit A: 0.5 ng/mL, kit B: 0.3 ng/mL...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-11-01 00:00:00
abstract::The aim was to compare two thyroglobulin-immunoradiometric assays (Tg-IRMA) in the follow-up of patients with differentiated thyroid carcinoma (DTC) in order to set up interassay correlation, correlation to clinical background, and to determine whether a lower functional sensitivity (kit A: 0.5 ng/mL, kit B: 0.3 ng/mL...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-11-01 00:00:00
abstract::Between 1984 and 1997, a total of 444 children (< 15 years) received an orthotopic liver transplant (OLT) at Saint-Luc University Clinics. Biliary atresia constituted the indication for OLT in 304 cases (68%). Median age (range) at OLT was 2.1 years (0.3-14.5). 177 children (40%) received a whole liver graft, 184 (41%...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-11-01 00:00:00