Abstract：
BACKGROUND:In order to optimize the tenogenic differentiation of mesenchymal stem cells (MSCs), researchers should consider various factors. However, this requires testing numerous experimental settings, which is costly and time-consuming. We aimed to assess the differential effects of transforming growth factor beta-3 (TGF-β3) on the tenogenesis of tonsil-derived MSCs (T-MSCs) and bone marrow-derived MSCs (BM-MSCs) using response surface methodology (RSM). METHODS:Bone marrow and tonsillar tissue were collected from four patients; mononuclear cells were separated and treated with 5 or 10 ng/mL of TGF-β3. A full factorial experimental design with a categorical factor of 0 was employed to study the effect of tension based on T-MSCs. Eighty-four trials were fitted with RSM and then used to obtain mathematical prediction models. RESULTS:Exposure of T-MSCs and BM-MSCs to TGF-β3 increased the expression of scleraxis (SCX), tenomodulin (TNMD), decorin, collagen I, and tenascin C. Expression of most of these factors reached a maximum after 2-3 days of treatment. The model predicted that the values of the tenocyte lineage-related factors assessed would be significantly increased at 2.5 days of culture with 2.7 ng/mL of TGF-β3 for T-MSCs and at 2.3 days of culture regardless of TGF-β3 concentration for BM-MSCs. CONCLUSIONS:This study demonstrated that the RSM prediction of the culture time necessary for the tenogenic differentiation of T-MSCs and BM-MSCs under TGF-β3 stimulation was similar to the experimentally determined time of peak expression of tenocyte-related mRNAs, suggesting the potential of using the RSM approach for optimization of the culture protocol for tenogenesis of MSCs.
journal_name
J Orthop Surg Resjournal_title
Journal of orthopaedic surgery and researchauthors
Kwon SS,Kim H,Shin SJ,Lee SYdoi
10.1186/s13018-020-01623-8subject
Has Abstractpub_date
2020-03-17 00:00:00pages
109issue
1issn
1749-799Xpii
10.1186/s13018-020-01623-8journal_volume
15pub_type
杂志文章abstract：BACKGROUND:Failure of pedicle screw fixation is often seen in patients with severe osteoporosis. We developed new lumbar spinal instrumentation (Tadpole system) for elderly patients who have osteoporotic bone and poor general health status. The objective of this study was to document the long-term clinical outcomes aft...
journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/1749-799X-9-33
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journal_title：Journal of orthopaedic surgery and research
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journal_title：Journal of orthopaedic surgery and research
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journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-019-1144-7
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journal_title：Journal of orthopaedic surgery and research
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journal_title：Journal of orthopaedic surgery and research
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journal_title：Journal of orthopaedic surgery and research
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journal_title：Journal of orthopaedic surgery and research
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journal_title：Journal of orthopaedic surgery and research
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journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-019-1521-2
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doi：10.1186/s13018-019-1399-z
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更新日期：2018-10-12 00:00:00
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更新日期：2020-02-28 00:00:00
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journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-019-1269-8
更新日期：2019-07-16 00:00:00
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journal_title：Journal of orthopaedic surgery and research
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doi：10.1186/s13018-016-0480-0
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journal_title：Journal of orthopaedic surgery and research
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pub_type： 杂志文章,随机对照试验
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journal_title：Journal of orthopaedic surgery and research
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journal_title：Journal of orthopaedic surgery and research
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doi：10.1186/s13018-018-0891-1
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journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
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journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-014-0139-7
更新日期：2014-12-31 00:00:00
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journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
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journal_title：Journal of orthopaedic surgery and research
pub_type： 杂志文章
doi：10.1186/s13018-019-1437-x
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