Abstract:
BACKGROUND:Renal proximal tubular sodium and glucose reabsorption are regulated by the sodium-glucose cotransporter (SGLT2). Changes in this transporter can play a role in hyperglycaemia and reactive oxygen species (ROS) production. We demonstrated increased glucose absorption in proximal tubule membrane vesicles and increased expression of SGLT2 in hypertensive rats. Here we investigated Angiotensin II (Ang II) -dependent SGLT2 expression induction and the role of SGLT2 induction in the development of Ang II-dependent kidney damage. The aim of this study was to determine whether SGLT2 induction by Ang II is associated with Ang II-dependent kidney damage. We propose the following objectives a) to demonstrate that Ang II induces SGLT2 expression and b) to demonstrate that prevention of SGLT2 expression and activity prevent Ang II-induced kidney damage. METHODS:We used chronic Ang II infusion as a model of kidney damage in male Wistar rats and evaluated systolic blood pressure by telemetric methods. SGLT2 mRNA and protein expression were evaluated by PCR and immunoblotting. SGLT2 activity was evaluated in brush border membrane vesicles by measuring glucose uptake. ROS production was measured by confocal microscopy. The glomerular filtration rate (GFR) was evaluated by the inulin excretion method, and urinary protein excretion was evaluated by the Bradford method. Biological parameter evaluations were performed, after two weeks of infusion of Ang II. We compared the effects of Angiotensin II (AT1) receptor blockade by Losartan and SGLT2 inhibition by Empagliflozin both as monotherapy treatments and in combination on the development of kidney damage. RESULTS:Chronic Ang II infusion led to a blood pressure elevation and increased SGLT2 mRNA expression and activity as well as kidney damage, as reflected by increased ROS production, decreased GFR and increased urinary protein excretion. AT1 receptor blockade prevented all these changes. By contrast, SGLT2 inhibition did not affect blood pressure and had a small effect on kidney damage. However, the combination of both drugs resulted in the potentiation of the effects observed by AT1 receptor blockade alone. CONCLUSIONS:We suggest that Ang II-dependent increased SGLT2 induction is one mechanism by which Ang II induces kidney damage.
journal_name
BMC Nephroljournal_title
BMC nephrologyauthors
Reyes-Pardo H,Bautista R,Vargas-Robles H,Rios A,Sánchez D,Escalante Bdoi
10.1186/s12882-019-1490-zsubject
Has Abstractpub_date
2019-08-02 00:00:00pages
292issue
1issn
1471-2369pii
10.1186/s12882-019-1490-zjournal_volume
20pub_type
杂志文章相关文献
BMC Nephrology文献大全abstract:BACKGROUND:This study describes chronic kidney disease of uncertain aetiology (CKDu), which cannot be attributed to diabetes, hypertension or other known aetiologies, that has emerged in the North Central region of Sri Lanka. METHODS:A cross-sectional study was conducted, to determine the prevalence of and risk factor...
journal_title:BMC nephrology
pub_type: 杂志文章
doi:10.1186/1471-2369-14-180
更新日期:2013-08-27 00:00:00
abstract:BACKGROUND:Despite the urgent need for evidence to guide the end-of-life (EOL) care for patients with chronic kidney disease (CKD), we have limited knowledge of the costs and intensity of EOL care in this population. The present study examined patterns and predictors for EOL care intensity among elderly patients with C...
journal_title:BMC nephrology
pub_type: 杂志文章
doi:10.1186/s12882-017-0456-2
更新日期:2017-01-26 00:00:00
abstract:BACKGROUND:Accurate estimation of glomerular filtration rate (GFR) is important for diagnosis and risk stratification in chronic kidney disease and for selection of living donors. Ethnic differences have required correction factors in the originally developed creatinine-based GFR estimation equations for populations ar...
journal_title:BMC nephrology
pub_type: 杂志文章
doi:10.1186/s12882-018-0813-9
更新日期:2018-02-01 00:00:00
abstract:BACKGROUND:Corticosteroids are widely used to reduce the urine protein levels of patients with immunoglobulin A nephropathy (IgAN). However, their potential preventive effects on end-stage kidney disease (ESKD) are unclear. METHODS:We previously performed a large-scale, long-term multicenter cohort study of patients w...
journal_title:BMC nephrology
pub_type: 杂志文章,多中心研究
doi:10.1186/s12882-018-1019-x
更新日期:2018-09-06 00:00:00
abstract:BACKGROUND:In view of the prevalence of oxidative stress in chronic kidney disease (CKD) patients, the loss of low-molecular-weight biomolecules by hemodialysis and the antioxidant potential of some uremic solutes that accumulate in CKD, we used in vitro model systems to test the antioxidant potential of the following ...
journal_title:BMC nephrology
pub_type: 杂志文章
doi:10.1186/s12882-015-0029-1
更新日期:2015-04-11 00:00:00
abstract:BACKGROUND:This study will assess measures of vascular health and inflammation in Aboriginal Australian adults with chronic kidney disease (CKD), and determine if intensive periodontal intervention improves cardiovascular health, progression of renal disease and periodontal health over a 24-month follow-up. METHODS:Th...
journal_title:BMC nephrology
pub_type: 杂志文章,随机对照试验
doi:10.1186/s12882-015-0169-3
更新日期:2015-10-31 00:00:00
abstract:INTRODUCTION:Urinary biomarkers of kidney injury are presumed to reflect renal tubular damage. However, their concentrations may be influenced by other factors, such as hematuria or pyuria. We sought to examine what non-injury related urinalysis factors are associated with urinary biomarker levels. METHODS:We examined...
journal_title:BMC nephrology
pub_type: 杂志文章,多中心研究
doi:10.1186/s12882-017-0629-z
更新日期:2017-07-06 00:00:00
abstract:BACKGROUND:Patients undergoing liver transplantation with preexisting renal dysfunction are prone to further renal impairment with the early postoperative use of Calcineurin-inhibitors. However, there is only little scientific evidence for the safety and efficacy of de novo CNI free "bottom-up" regimens in patients wit...
journal_title:BMC nephrology
pub_type: 临床试验,杂志文章
doi:10.1186/1471-2369-11-24
更新日期:2010-09-14 00:00:00
abstract:BACKGROUND:Ongoing efforts are focused on shortening ischemia intervals as much as possible during partial nephrectomy to preserve renal function. Off-clamp partial nephrectomy (off-PN) has been a common strategy for to avoid ischemia in small renal tumors. Although studies comparing the advantages between off-PN with ...
journal_title:BMC nephrology
pub_type: 杂志文章,meta分析
doi:10.1186/s12882-018-0993-3
更新日期:2018-07-31 00:00:00
abstract:BACKGROUND:Chronic hepatitis B virus (HBV) infection and chronic kidney disease (CKD) have high prevalences in Taiwan and worldwide. However, the association of untreated chronic hepatitis B virus (HBV) infection with chronic kidney disease (CKD) remains unclear. METHODS:This cohort study used claims data in the Taiwa...
journal_title:BMC nephrology
pub_type: 杂志文章
doi:10.1186/s12882-015-0106-5
更新日期:2015-07-22 00:00:00
abstract:BACKGROUND:Patients with end-stage renal disease (ESRD) under hemodialysis (HD) are at greater risks of infectious spondylitis (IS), but there is no reliable predictor that facilitate early detection of this relatively rare and insidious disease. METHODS:A retrospective review of the medical records from patients with...
journal_title:BMC nephrology
pub_type: 杂志文章
doi:10.1186/s12882-019-1504-x
更新日期:2019-08-16 00:00:00
abstract:BACKGROUND:Liddle's syndrome is a rare monogenic form of hypertension caused by truncating or missense mutations in the C termini of the epithelial sodium channel (ENaC) β or γ subunits. Patients with this syndrome present with early onset of hypertension, hypokalemia, metabolic alkalosis, hyporeninemia and hypoaldoste...
journal_title:BMC nephrology
pub_type: 杂志文章
doi:10.1186/s12882-018-0916-3
更新日期:2018-05-23 00:00:00
abstract:BACKGROUND:Autosomal dominant polycystic kidney disease (ADPKD) is the leading inheritable cause of end-stage renal disease (ESRD); however, the natural course of disease progression is heterogeneous between patients. This study aimed to develop a natural history model of ADPKD that predicted progression rates and long...
journal_title:BMC nephrology
pub_type: 杂志文章
doi:10.1186/s12882-017-0804-2
更新日期:2018-02-13 00:00:00
abstract:BACKGROUND:Sickle cell trait is not completely benign, and some renal complications can occur. The baseline rate of admission for gross hematuria in normal males carrying the sickle cell trait is 2%. CASE PRESENTATION:A 35-year-old non-smoking African man experienced a 2-week history of painless, profuse and persisten...
journal_title:BMC nephrology
pub_type: 杂志文章
doi:10.1186/s12882-018-0939-9
更新日期:2018-06-08 00:00:00
abstract:BACKGROUND:Family members of patients with end stage renal disease were reported to have an increased prevalence of chronic kidney disease (CKD). However, studies differentiated genetic and non-genetic family members are limited. We sought to investigate the prevalence of CKD among fist-degree relatives and spouses of ...
journal_title:BMC nephrology
pub_type: 杂志文章,多中心研究
doi:10.1186/1471-2369-14-19
更新日期:2013-01-18 00:00:00
abstract:BACKGROUND:Observational studies have demonstrated an association between nephrolithiasis and hypertension. The aim of this meta-analysis was to summarize all available evidence. METHODS:PubMed, EMBASE, the Cochrane Central Register of Controlled Trials databases, and the reference lists of relevant articles were sear...
journal_title:BMC nephrology
pub_type: 杂志文章,meta分析
doi:10.1186/s12882-017-0762-8
更新日期:2017-11-29 00:00:00
abstract:BACKGROUND:Heavy metal poisoning can cause debilitating illness if left untreated, and its management in anuric patients poses challenges. Literature with which to guide clinical practice in this area is rather scattered. CASE PRESENTATION:We present a case of symptomatic lead and arsenic poisoning from use of Ayurved...
journal_title:BMC nephrology
pub_type: 杂志文章,评审
doi:10.1186/s12882-019-1561-1
更新日期:2019-10-17 00:00:00
abstract:BACKGROUND:IgA nephropathy (IgAN) is the most common glomerulonephritis worldwide and is an important cause of end-stage renal disease (ESRD). Exploring novel biomarkers is necessary for predicting the disease activity and progression of IgAN patients. The present study sought to investigate the value of serum C4 for p...
journal_title:BMC nephrology
pub_type: 杂志文章
doi:10.1186/s12882-019-1420-0
更新日期:2019-07-04 00:00:00
abstract:BACKGROUND:Cool dialysate is often recommended for prevention of intra-dialytic hypotensive episodes in maintenance hemodialysis (HD) patients. However, its effect on toxin removal is not studied. It is known that inter-compartmental resistance is the main barrier for toxin removal. Cool dialysate can potentially incre...
journal_title:BMC nephrology
pub_type: 杂志文章,随机对照试验
doi:10.1186/s12882-015-0017-5
更新日期:2015-02-27 00:00:00
abstract:BACKGROUND:Bioimpedance analysis (BIA) has been reported as helpful in identifying hypervolemia. Observation data showed that hypervolemic maintenance hemodialysis (MHD) patients identified using BIA methods have higher mortality risk. However, it is not known if BIA-guided fluid management can improve MHD patients' su...
journal_title:BMC nephrology
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1186/1471-2369-13-120
更新日期:2012-09-25 00:00:00
abstract:BACKGROUND:The management of proliferative lupus nephritis (LN) comprises timely and coordinated immunosuppressive therapy. This study aimed to evaluate and compare the effectiveness and safety profile of low dose mycophenolate mofetil (MMF) and cyclophosphamide (CYC) in induction therapy of LN in Nepalese population. ...
journal_title:BMC nephrology
pub_type: 杂志文章,随机对照试验
doi:10.1186/s12882-018-0973-7
更新日期:2018-07-11 00:00:00
abstract:BACKGROUND:Recipients of living donor renal transplantation are typically considered to have a relatively lower immunological risk. This retrospective study aimed to compare the therapeutic efficacy and safety between rabbit antithymocyte globulin (rATG) or interleukin-2 receptor antagonist (IL2-RA) induction therapies...
journal_title:BMC nephrology
pub_type: 杂志文章
doi:10.1186/s12882-019-1293-2
更新日期:2019-03-22 00:00:00
abstract:BACKGROUND:Whether persistent hematuria in patients with antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) during clinical remission reflects active disease or chronic glomerular injury is uncertain. This study aimed to investigate the significance of persistent hematuria during clinical remission ...
journal_title:BMC nephrology
pub_type: 杂志文章
doi:10.1186/s12882-017-0763-7
更新日期:2017-12-06 00:00:00
abstract:BACKGROUND:Treatment of idiopathic membranous nephropathy with nephrotic syndrome is still controversial. There is currently little known about the clinical use of renal biomarkers which may explain contradictory results obtained from clinical trials. In order to assess whether IgG-uria can predict the outcome in membr...
journal_title:BMC nephrology
pub_type: 临床试验,杂志文章
doi:10.1186/1471-2369-15-74
更新日期:2014-05-08 00:00:00
abstract:BACKGROUND:The product of the concentrations of urinary tissue inhibitor of metalloproteinases-2 and insulin-like growth factor binding protein-7 (urinary [TIMP-2] × [IGFBP-7]) has been suggested as biomarker for early detection of acute kidney injury (AKI) in various clinical settings. However, the performance of urin...
journal_title:BMC nephrology
pub_type: 杂志文章
doi:10.1186/s12882-019-1456-1
更新日期:2019-07-17 00:00:00
abstract:BACKGROUND:There is limited knowledge of Chronic Kidney Disease (CKD) among high risk populations, especially in the developing countries. We report our study of testing for CKD in at-risk subjects. METHODS:In a cross-sectional study, 527 people from primary and secondary health care areas in the city of Kinshasa were...
journal_title:BMC nephrology
pub_type: 杂志文章
doi:10.1186/1471-2369-10-18
更新日期:2009-07-21 00:00:00
abstract:BACKGROUND:FGF23 controls serum l,25(OH)2D3 levels and phosphate homeostasis. This study evaluates the effects of ferritin on intact PTH, FGF23, and l,25(OH)2D3 in patients with major thalassemia. It also evaluates FGF23 changes in patients with hypoparathyroidism to clarify the interaction between FGF23 and PTH in the...
journal_title:BMC nephrology
pub_type: 杂志文章
doi:10.1186/s12882-020-02101-3
更新日期:2020-11-16 00:00:00
abstract:BACKGROUND:There is conflicting evidence of benefit versus harm for warfarin anticoagulation in hemodialysis patients with atrial fibrillation. This equipoise may be explained by suboptimal Time in Therapeutic Range (TTR), which correlates well with thromboembolic and bleeding complications. This study aimed to compare...
journal_title:BMC nephrology
pub_type: 杂志文章,多中心研究
doi:10.1186/s12882-017-0809-x
更新日期:2018-01-08 00:00:00
abstract:BACKGROUND:The possibility of clearing Cell-free Plasma Hemoglobin (CPH) from human plasma may appear attractive, especially when considering the noxious effects that CPH has on the immune function and the renal damage caused by its filtration. The existence of the so-called High Cut-Off (HCO) filters, possessing pores...
journal_title:BMC nephrology
pub_type: 杂志文章
doi:10.1186/s12882-018-1051-x
更新日期:2018-10-04 00:00:00
abstract:BACKGROUND:Recently there has been a progressive loss of specialty related skills for nephrologists. Among the skills we find the kidney biopsy that has a central role in diagnosis of renal parenchymal disease. One of the causes might be the belief that the kidney biopsy should be performed only in larger Centers which...
journal_title:BMC nephrology
pub_type: 杂志文章
doi:10.1186/s12882-017-0796-y
更新日期:2018-01-15 00:00:00