Abstract:
BACKGROUND:The tumor draining lymph node concept was first described in penile cancer for staging. Immunohistochemistry and histopathology evaluations are routinely used in clinical practice to examine lymph nodes for metastasis. However, these methods are time-consuming with low diagnostic accuracy and micro-metastases might be missed. In this study, we aim to evaluate detection of metastatic cells in draining lymph nodes by flow cytometry. METHODS:To assess the sensitivity of micro-metastasis detection by FACS (Fluorescence-activated cell sorting), HeLa cells were titrated into Peripheral blood mononuclear cells (PBMCs) and expression of pan-cytokeratin AE1/AE3 was analyzed. Single cell suspensions were separately prepared from 10 regional lymph nodes obtained from 5 patients with invasive penile cancer undergoing radical surgery and lymph node dissection. Lymph node dereived cells were examined for cell surface expression of EpCAM, E-cadherin and intracellular expression of pan-cytokeratin AE1/AE3 by FACS. RESULTS:Ten lymph nodes from 5 penile cancer patients were investigated in a head-to-head comparison between FACS and pathology examination of sections. All metastatic lymph nodes verified by pathology examination were also identified by FACS. Two additional lymph nodes with micro-metastases were diagnosed by FACS only. CONCLUSIONS:FACS analyses of pan-cytokeratin AE1/AE3 stained single cells from tumor draining lymph nodes can be used to detect micro-metastases in patients with penile cancer patients.
journal_name
BMC Uroljournal_title
BMC urologyauthors
Zhang L,Hu J,Ali Zirakzadeh A,Rosvall J,Hedlund M,Hu PS,P A Wallin R,Sherif A,Winqvist Odoi
10.1186/s12894-018-0399-3subject
Has Abstractpub_date
2018-10-05 00:00:00pages
86issue
1issn
1471-2490pii
10.1186/s12894-018-0399-3journal_volume
18pub_type
杂志文章相关文献
BMC Urology文献大全abstract:BACKGROUND:The purpose of this study was to explore the budget impact of dutasteride plus tamsulosin fixed-dose combination (DUT + TAM FDC) versus tamsulosin monotherapy, in the treatment of patients with benign prostatic hyperplasia (BPH) from the perspective of the Greek healthcare insurance system. METHODS:A Micros...
journal_title:BMC urology
pub_type: 杂志文章
doi:10.1186/1471-2490-14-78
更新日期:2014-09-26 00:00:00
abstract:BACKGROUND:Osteoporosis is a common consequence of androgen deprivation therapy (ADT) for prostate cancer. Up to 20 % of men on ADT have suffered from fractures within 5 years. The WHO Fracture Risk Assessment Tool (FRAX) has been utilized to predict the 10-year probability of major osteoporotic and hip fracture. Howev...
journal_title:BMC urology
pub_type: 杂志文章
doi:10.1186/s12894-016-0151-9
更新日期:2016-06-17 00:00:00
abstract:BACKGROUND:The indications for nephron-sparing surgery are expanding constantly. One major contributing fact for this development is the improvement of haemostatic techniques following excision of the tumor. Nevertheless, postoperative bleeding complications still occur. To prevent this, we prospectively studied the ef...
journal_title:BMC urology
pub_type: 杂志文章
doi:10.1186/1471-2490-8-8
更新日期:2008-04-29 00:00:00
abstract:BACKGROUND:Obstetric fistula although virtually eliminated in high income countries, still remains a prevalent and debilitating condition in many parts of the developing world. It occurs in areas where access to care at childbirth is limited, or of poor quality and where few hospitals offer the necessary corrective sur...
journal_title:BMC urology
pub_type: 杂志文章
doi:10.1186/1471-2490-11-23
更新日期:2011-12-07 00:00:00
abstract:BACKGROUND:This multicenter, randomized, double-blind, double-dummy, placebo-controlled trial primarily evaluated the efficacy of tadalafil once-daily (OaD) or on-demand ("pro-re-nata"; PRN) treatment, started early post-nsRP. Secondary outcome-measures on quality-of-life (QoL) and treatment satisfaction are reported. ...
journal_title:BMC urology
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1186/s12894-015-0022-9
更新日期:2015-04-12 00:00:00
abstract:BACKGROUND:We evaluated the clinical outcome of low grade Ta bladder cancer followed-up for a long period using the 2004 WHO grading system. METHODS:We retrospectively reviewed 190 patients with primary, low grade Ta bladder cancer. We defined worsening progression (WP) as confirmed high grade Ta, all T1 or Tis/concom...
journal_title:BMC urology
pub_type: 杂志文章
doi:10.1186/1471-2490-14-5
更新日期:2014-01-08 00:00:00
abstract:BACKGROUND:Emerging evidence has noted the important participation of microRNAs (miRNAs) in several human diseases including cancer. This research was launched to probe the function of miR-381 in bladder cancer (BCa) progression. METHODS:Twenty-eight patients with primary BCa were included in this study. Cancer tissue...
journal_title:BMC urology
pub_type: 杂志文章
doi:10.1186/s12894-020-00775-3
更新日期:2021-01-06 00:00:00
abstract:BACKGROUND:Our understanding of early post-vasectomy recanalization is limited to histopathological studies. The objective of this study was to estimate the frequency and to describe semen analysis patterns of early recanalization after vasectomy. METHODS:Charts displaying serial post-vasectomy semen analyses were cre...
journal_title:BMC urology
pub_type: 杂志文章,随机对照试验
doi:10.1186/1471-2490-6-25
更新日期:2006-09-19 00:00:00
abstract:BACKGROUND:Clear cell sarcoma of the kidney (CCSK) in adults is extremely rare. Optimal treatment of adult patients with CCSK remains unclear. CASE PRESENTATION:A 22-year-old man presented with a 2-month history of left flank pain. A color duplex sonography revealed a hypervascular, heterogeneous renal mass. Abdominal...
journal_title:BMC urology
pub_type: 杂志文章
doi:10.1186/1471-2490-6-11
更新日期:2006-04-03 00:00:00
abstract:BACKGROUND:Over the last few years the number of flexible ureterorenoscopies, used for renal stone treatment, has risen steadily. This was associated with an increase in costs for maintenance and repair of the fragile ureterorenoscopes used. To overcome this problem single-use devices have been introduced to the market...
journal_title:BMC urology
pub_type: 杂志文章
doi:10.1186/s12894-020-00624-3
更新日期:2020-05-18 00:00:00
abstract:BACKGROUND:Bladder hyperthermic intracavitary chemotherapy (HIVEC) has good effectiveness for bladder cancer, but conventional HIVEC systems lack precision and convenient application. To test the safety of a new HIVEC device (BR-TRG-II-type) in pigs and to perform a preliminary clinical trial in patients with bladder c...
journal_title:BMC urology
pub_type: 临床试验,杂志文章
doi:10.1186/s12894-019-0543-8
更新日期:2019-12-03 00:00:00
abstract:BACKGROUND:To assess factors that can predict active surveillance (AS) failure on serial transrectal ultrasound guided biopsies in patients with low-risk prostate cancer. METHODS:We evaluated the records of 144 consecutive patients enrolled in AS between 2007 and 2014 at a single academic institution. Low risk inclusi...
journal_title:BMC urology
pub_type: 杂志文章
doi:10.1186/s12894-019-0452-x
更新日期:2019-04-23 00:00:00
abstract:BACKGROUND:Laparoscopic single-site surgery has recently emerged in the field of urology and this minimally-invasive surgery has resulted in a further reduction in morbidity compared with traditional laparoscopy. We present our initial experience with laparoendoscopic single-site surgery of partial adrenalectomy (LESS-...
journal_title:BMC urology
pub_type: 杂志文章
doi:10.1186/1471-2490-10-19
更新日期:2010-11-23 00:00:00
abstract:BACKGROUND:To examine the expression of type 1 plasminogen inhibitor (PAI-1) in clear cell renal cell carcinoma (CCRCC), and its possible association with microvessel density (MVD), the expression of thrombospondin-1 (TSP-1), nuclear grade, tumour stage, continuously coded tumour size (CCTS) and to assess the value of ...
journal_title:BMC urology
pub_type: 临床试验,杂志文章
doi:10.1186/1471-2490-10-20
更新日期:2010-12-03 00:00:00
abstract:BACKGROUND:More and more frequently stress urinary incontinence affects young healthy women. Hence, early implementation of effective preventive strategies in nulliparous continent women is essential, including pelvic floor muscle training. An initial evaluation based on the bioelectrical activity of the pelvic floor m...
journal_title:BMC urology
pub_type: 杂志文章,随机对照试验
doi:10.1186/s12894-015-0103-9
更新日期:2015-10-24 00:00:00
abstract:BACKGROUND:Buried penis, most commonly seen in children, is particularly debilitating in adults, resulting in inability to void while standing and it also affects vaginal penetration. We report a case of buried penis due to a traffic accident, which caused dislocation of the fractured pubic bone that shifted inside and...
journal_title:BMC urology
pub_type: 杂志文章
doi:10.1186/1471-2490-4-6
更新日期:2004-06-08 00:00:00
abstract:BACKGROUND:Renal cell carcinoma can cause various paraneoplastic syndromes including metabolic and hematologic disturbances. Paraneoplastic hypereosinophilia has been reported in a variety of hematologic and solid tumors. We present the first case in the literature of severe paraneoplastic hypereosinophilia in a patien...
journal_title:BMC urology
pub_type: 杂志文章
doi:10.1186/1471-2490-12-7
更新日期:2012-03-21 00:00:00
abstract:BACKGROUND:Retzius-sparing robot-assisted laparoscopic radical prostatectomy (rsRARP) allows entire prostatectomy procedure via the pouch of Douglas. In low- and intermediate-risk prostate cancer (PCa) there is level 1 evidence that the Retzius-sparing approach impacts early continence recovery. Since specific data on ...
journal_title:BMC urology
pub_type: 杂志文章
doi:10.1186/s12894-019-0550-9
更新日期:2019-11-12 00:00:00
abstract:BACKGROUND:Metachronous renal cell carcinoma after radical nephrectomy is extremely rare. Renal cell carcinoma commonly metastasizes to distant organs. However, metastasis to the urinary bladder is very uncommon. CASE PRESENTATION:Herein, we report a case of metachronous renal cell carcinoma with metastasis to the uri...
journal_title:BMC urology
pub_type: 杂志文章
doi:10.1186/s12894-019-0521-1
更新日期:2019-12-27 00:00:00
abstract:BACKGROUND:Kidney cancer is a lethal neoplasm that affects several thousands of people every year. Renal cell carcinoma (RCC) is the most common histologic type. Recent developments in the therapeutic approach include antiangiogenic targeted approaches and Immunotherapy. Thus, the therapeutic algorithm of RCC patients ...
journal_title:BMC urology
pub_type: 杂志文章
doi:10.1186/s12894-020-00618-1
更新日期:2020-06-02 00:00:00
abstract:BACKGROUND:Metastatic tumors of the penis are uncommon, and fewer than 500 cases have been reported since 1870. Most penile secondary tumors originate in organs of the genitourinary tract, followed by the gastrointestinal tract. Primary tumors of sarcoma origin are extremely rare. Herein, we present a teenager who had ...
journal_title:BMC urology
pub_type: 杂志文章
doi:10.1186/s12894-019-0511-3
更新日期:2019-09-02 00:00:00
abstract:BACKGROUND:Urinary tract infection (UTI) is very common in patients with urolithiasis, which makes the treatment of urolithiasis complicated, even dangerous. The objective of this study was to determine the risk factors for UTI in patients with urolithiasis. METHODS:Eight hundred six patients with urolithiasis were re...
journal_title:BMC urology
pub_type: 杂志文章
doi:10.1186/s12894-018-0359-y
更新日期:2018-05-21 00:00:00
abstract:BACKGROUND:A supernumerary kidney (SK) is an additional kidney with its own capsule and blood supply that is not fused with the ipsilateral kidney (IK). Because individual case reports indicate a high morbidity rate, the aim of this retrospective study was a detailed analysis of this rare anatomical variant. METHODS:O...
journal_title:BMC urology
pub_type: 杂志文章
doi:10.1186/s12894-019-0522-0
更新日期:2019-10-17 00:00:00
abstract::This review summarizes recent literature on advances regarding renal and ureteral calculi, with particular focus in areas of recent advances in the overall field of urolithiasis. Clinical management in everyday practice requires a complete understanding of the issues regarding metabolic evaluation and subgrouping of s...
journal_title:BMC urology
pub_type: 杂志文章,评审
doi:10.1186/1471-2490-13-10
更新日期:2013-02-16 00:00:00
abstract:BACKGROUND:Congenital mid-ureteral stricture is a rare malformation of the ureter leading to prenatal and neonatal hydronephrosis. Site characterization of the narrowing is important to optimize the surgical approach to the newborn affected by hydronephrosis. CASE PRESENTATION:We report a female EM with a rare form of...
journal_title:BMC urology
pub_type: 杂志文章
doi:10.1186/1471-2490-7-8
更新日期:2007-06-08 00:00:00
abstract:BACKGROUND:Continuous bladder irrigation (CBI) and proper adjustment of saline irrigation speed are important to avoid CBI failure in hemorrhagic cystitis (HC) patients after allogeneic hematopoietic stem cell transplantation (HSCT). Nevertheless, too fast irrigation speed could take away the patient's much heat, contr...
journal_title:BMC urology
pub_type: 杂志文章
doi:10.1186/s12894-020-00757-5
更新日期:2020-11-10 00:00:00
abstract:BACKGROUND:The diagnostic pathway for prostate cancer (PCa) is advancing towards an imaging-driven approach. Multiparametric magnetic resonance imaging, although increasingly used, has not shown sufficient accuracy to replace biopsy for now. The introduction of new ultrasound (US) modalities, such as quantitative contr...
journal_title:BMC urology
pub_type: 临床试验,杂志文章
doi:10.1186/s12894-018-0409-5
更新日期:2018-11-08 00:00:00
abstract:BACKGROUND:Radical prostatectomy (RP) is a common treatment for prostate cancer (PCa). Morbidity, mortality and pathological outcomes may be superior in academic institutions. One explanation may be the involvement of oncology fellowship trained urologists within academic institutions. The literature examining patholog...
journal_title:BMC urology
pub_type: 杂志文章
doi:10.1186/1471-2490-14-82
更新日期:2014-10-23 00:00:00
abstract:BACKGROUND:Polyorchidism is a congenital anomaly of the urogenital system and means more than two testes. It is a rare phenomenon, where there are no more than 200 reported cases in the literature. In this case, we report a 13-month year's old case with five testicles. CASE PRESENTATION:We report a rare five testicles...
journal_title:BMC urology
pub_type: 杂志文章
doi:10.1186/s12894-020-00681-8
更新日期:2020-08-20 00:00:00
abstract:BACKGROUND:The objective of this study was to describe overall survival and the management of men with favorable risk prostate cancer (PCa) within a large community-based health care system in the United States. METHODS:A retrospective cohort study was conducted using linked electronic health records from men aged ≥40...
journal_title:BMC urology
pub_type: 杂志文章
doi:10.1186/s12894-018-0372-1
更新日期:2018-06-04 00:00:00