Abstract:
BACKGROUND:The purpose of the study is to investigate whether autologous platelet-rich plasma (PRP) can serve as bone-inducing factors to provide osteoinduction and improve bone regeneration for tissue-engineered bones fabricated with bone marrow mesenchymal stem cells (MSCs) and beta-tricalcium phosphate (β-TCP) ceramics. The current study will give more insight into the contradictory osteogenic capacity of PRP. METHODS:The concentration of platelets, platelet-derived growth factor-AB (PDGF-AB), and transforming growth factor-β1 (TGF-β1) were measured in PRP and whole blood. Tissue-engineered bones using MSCs on β-TCP scaffolds in combination with autologous PRP were fabricated (PRP group). Controls were established without the use of autologous PRP (non-PRP group). In vitro, the proliferation and osteogenic differentiation of MSCs on fabricated constructs from six rabbits were evaluated with MTT assay, alkaline phosphatase (ALP) activity, and osteocalcin (OC) content measurement after 1, 7, and 14 days of culture. For in vivo study, the segmental defects of radial diaphyses of 12 rabbits from each group were repaired by fabricated constructs. Bone-forming capacity of the implanted constructs was determined by radiographic and histological analysis at 4 and 8 weeks postoperatively. RESULTS:PRP produced significantly higher concentration of platelets, PDGF-AB, and TGF-β1 than whole blood. In vitro study, MTT assay demonstrated that the MSCs in the presence of autologous PRP exhibited excellent proliferation at each time point. The results of osteogenic capacity detection showed significantly higher levels of synthesis of ALP and OC by the MSCs in combination with autologous PRP after 7 and 14 days of culture. In vivo study, radiographic observation showed that the PRP group produced significantly higher score than the non-PRP group at each time point. For histological evaluation, significantly higher volume of regenerated bone was found in the PRP group when compared with the non-PRP group at each time point. CONCLUSIONS:Our study findings support the osteogenic capacity of autologous PRP. The results indicate that the use of autologous PRP is a simple and effective way to provide osteoinduction and improve bone regeneration for tissue-engineered bone reconstruction.
journal_name
J Orthop Surg Resjournal_title
Journal of orthopaedic surgery and researchauthors
Yu T,Pan H,Hu Y,Tao H,Wang K,Zhang Cdoi
10.1186/s13018-017-0665-1subject
Has Abstractpub_date
2017-11-21 00:00:00pages
178issue
1issn
1749-799Xpii
10.1186/s13018-017-0665-1journal_volume
12pub_type
杂志文章abstract:PURPOSE:The purpose of this study was to compare the interfragmentary compression force across a simulated scaphoid fracture by two commonly used compression screw systems; the Acutrak 2 Standard and the 3.0 mm Synthes headless compression screw. METHODS:Sixteen (8 pairs; 6 female, 2 male) cadaver scaphoids were rando...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/1749-799X-6-27
更新日期:2011-06-07 00:00:00
abstract:BACKGROUND:Osteoarthritis (OA), the most prevalent disease of articulating joints, is a complex multifactorial disease caused by genetic, mechanical, and environmental factors. In this research, we evaluated miRNA expression in OA. METHODS:Forty tissue samples from 29 patients undergoing joint replacement for OA were ...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-017-0542-y
更新日期:2017-03-28 00:00:00
abstract:BACKGROUND:Minimally invasive surgical approaches are widely used for total hip arthroplasty (THA). However, potential problems related to a reduced visual field during surgery, such as implant malposition, neurovascular injury, and poor implant fixation, have been reported. In these situations, a shorter stem is easie...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-015-0192-x
更新日期:2015-04-21 00:00:00
abstract:BACKGROUND:To date, there has been no adequate biomechanical model that would allow a quantitative comparison in terms of stability/stiffness between a corpectomy with the posterior column preserved and a total spondylectomy with the posterior column sacrificed. The objective of this study was to perform a biomechanica...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-015-0240-6
更新日期:2015-07-01 00:00:00
abstract:BACKGROUND:This study aimed to explore the relationship among different cervical sagittal parameters in asymptomatic volunteers and the correlation between surgical efficacy and difference of presumed and actual postoperative C2-7 Cobbs's angle (C2-7COBB), which was calculated based on preoperative T1 slope (T1S) in pa...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-020-02016-7
更新日期:2020-11-05 00:00:00
abstract:BACKGROUND:Degloving injuries are surgical conditions in which an extensive portion of skin and subcutaneous tissue is detached from the underlying fasciae, muscles, or bone surface. Frequently, there is an association of fracture underlying the degloved area. We aimed to compare the short-term outcomes of degloving in...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-017-0706-9
更新日期:2018-01-05 00:00:00
abstract:BACKGROUND:We aimed to explore the effects of polypharmacy and specific drug classes on readmissions and mortality after hip surgery. METHODS:We analyzed data on 272 consecutive hip fracture patients (72.1% females; age 82 ± 9 years) who underwent acute hip replacement. We collected detailed data on the pharmacologica...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-016-0486-7
更新日期:2016-11-24 00:00:00
abstract::This review analyzes the literature of bone grafts and introduces tissue engineering as a strategy in this field of orthopedic surgery. We evaluated articles concerning bone grafts; analyzed characteristics, advantages, and limitations of the grafts; and provided explanations about bone-tissue engineering technologies...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章,评审
doi:10.1186/1749-799X-9-18
更新日期:2014-03-17 00:00:00
abstract:BACKGROUND:The purpose of this study was to assess the impact of uniform anti-rotational proximal femoral intramedullary nail (APFN) use on patient mobility status with the treatment of two different unstable intertrochanteric femur fracture groups of geriatric patients. METHODS:The study included patients aged > 65 y...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-018-0911-1
更新日期:2018-08-22 00:00:00
abstract:BACKGROUND:This study was aimed at investigating the causes of lower extremity weaknesses after posterior lumbar spine fusion surgery and looking at subsequent treatment strategies. METHODS:Patients who underwent posterior lumbar spine fusion surgery in the Peking University First Hospital between January 2009 and Dec...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-020-01948-4
更新日期:2020-09-22 00:00:00
abstract:BACKGROUND:Open reduction with internal fixation (ORIF) and percutaneous surgery (PS) are the most common surgical procedures for the treatment of displaced intra-articular calcaneal fractures. The purpose of this retrospective study was to compare the clinical and radiological results of these techniques and to verify...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-016-0426-6
更新日期:2016-08-22 00:00:00
abstract:BACKGROUND:The Latarjet procedure addresses recurrent anterior shoulder instability in the context of a significant bony defect. However, the bony and soft tissue anatomy of the coracoid in coracoid transfer procedures has not yet been defined in Mongolian men. The aims of this study were to describe the soft tissue at...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-016-0461-3
更新日期:2016-10-24 00:00:00
abstract:BACKGROUND:Ossification of the posterior longitudinal ligament (OPLL) of the spine is a complex, multifactorial disease. Although several genes that are linked to cervical OPLL susceptibility have been reported, specific genetic studies regarding thoracic OPLL are lacking. Whole-genome sequencing has been considered as...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-018-1022-8
更新日期:2018-12-22 00:00:00
abstract:BACKGROUND:A tourniquet is a device commonly used to control massive hemorrhage during knee replacement surgery. However, the question remains whether the use of tourniquets affects the permeability of the bone cement around the knee prosthesis. Moreover, the long-term effects and stability of the knee prosthesis are s...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-020-02106-6
更新日期:2020-12-11 00:00:00
abstract:BACKGROUND:The medial patellofemoral ligament (MPFL) works in association with the medial patellotibial ligament (MPTL) and the medial patellomeniscal ligament (MPML) to impart stability to the patellofemoral joint. The anatomy and biomechanical characteristics of the MPFL have been well described but little is known a...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章,评审
doi:10.1186/s13018-020-02072-z
更新日期:2020-11-12 00:00:00
abstract:BACKGROUND:The aim of this study was to analyze the pattern and types of sensory nerve endings in ankle collateral ligaments using histological techniques, in order to observe the morphology and distribution of mechanoreceptors in the collateral ligaments of cadaver ankle joint, and to provide the morphological evidenc...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-015-0215-7
更新日期:2015-06-12 00:00:00
abstract:BACKGROUND:Sensory nerve endings in ligaments play an important role for the proprioceptive function. Clinical trials show that the sense of body position does not fully recover in the knee joint after reconstructive surgery of the ruptured anterior cruciate ligament. The aim of this study is to identify sensory corpus...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-020-01785-5
更新日期:2020-07-17 00:00:00
abstract::We write in response to Lawton et al.'s (J Orthop Surg Res 12:76, 2017) important systematic review comparing the outcomes of total ankle replacement (TAR) and ankle arthrodesis (AA) after reviewing the existing literature. Traditionally, AA was the gold standard treatment for ankle osteoarthritis but there is renewed...
journal_title:Journal of orthopaedic surgery and research
pub_type: 信件
doi:10.1186/s13018-019-1190-1
更新日期:2019-05-20 00:00:00
abstract::Submuscular and minimally invasive plate insertion is gaining popularity reducing the need for large open approaches and resulting in a smaller operative 'footprint.' With pediatric fractures and titanium implants, fibrous and osseous ingrowth to the implant and osseous implant integration may interfere with implant r...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/1749-799X-8-21
更新日期:2013-07-11 00:00:00
abstract:BACKGROUND:Cervical sagittal parameters were closely related with clinical outcomes after multi-level ACDF. Our purpose was to evaluate the clinical outcomes and cervical sagittal parameters in patients with MCSM after ACDF and to identify the risk factors of poor clinical outcomes. MATERIAL AND METHODS:ACDF was perfo...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-019-1504-3
更新日期:2019-12-11 00:00:00
abstract:BACKGROUND:Attempts have been made to validate the significance of remnant preservation with anterior cruciate ligament (ACL) reconstruction using immunohistochemical and immunocytochemical techniques. The purpose of this study was to examine the expression of mechanoreceptors in the remnant tissue of ACL reconstructio...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-017-0593-0
更新日期:2017-06-14 00:00:00
abstract:BACKGROUND:The aim of this research is to analyze the influence of Modic types on the clinical results of cervical spondylotic myelopathy treated by anterior cervical diskectomy and fusion. METHODS:A total of 106 patients with a mean age of 55.8 ± 6.5 years were included in this study. Patients with Modic changes were...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-014-0146-8
更新日期:2015-01-28 00:00:00
abstract:BACKGROUND:The transoral atlantoaxial reduction plate (TARP) is an effective advance in the treatment of atlantoaxial dislocation (AAD) and can enable the performance of anterior atlantoaxial release, reduction, decompression, and internal fixation in a one-stage operation. However, accurate transoral C2 pedicle insert...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章,随机对照试验
doi:10.1186/s13018-019-1189-7
更新日期:2019-05-16 00:00:00
abstract:OBJECTIVE:To assess the symptomatic effectiveness and safety of oral symptomatic slow-acting drugs (SYSADOAs) on the treatment of knee and/or hip osteoarthritis, such as chondroitin, glucosamine, and combination treatment with chondroitin plus glucosamine. METHODS:We searched electronic database including PubMed, Emba...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章,meta分析,评审
doi:10.1186/s13018-018-0871-5
更新日期:2018-07-06 00:00:00
abstract:BACKGROUND:Lubrication function is impaired and the lubricant content of synovial fluid (SF) changes immediately after plateau tibia fractures. Here, we aimed to analyze the lubricant content of SF at chronic term following plateau tibia fracture. METHODS:Forty-eight surgically treated patients without joint incongrue...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-015-0232-6
更新日期:2015-06-03 00:00:00
abstract:BACKGROUND:This study was undertaken to establish a rat bipedal walking model of cervical kyphosis (CK) associated with chronic forward flexed neck and assess the effects of chronic forward flexed neck on endplate chondrocytes. METHODS:Forty-eight 1-month-old Sprague-Dawley rats were randomly divided into 3 groups: fo...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-020-02124-4
更新日期:2021-01-04 00:00:00
abstract:BACKGROUND:Tibial plateau fracture (TPF) includes different fracture patterns with varied degrees of articular depression and displacement. Many kinds of fixators, including newly designed plate with locking screws, were applied to treat these complicated fractures. We intended to follow up the surgical outcomes of (1)...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-014-0062-y
更新日期:2014-07-20 00:00:00
abstract:BACGROUND:Although the contribution of fibroblast growth factor (FGF)-2 and mesenchymal stromal cells (MSCs) to bone formation is well known, few studies have investigated the combination of an autologous bone graft with FGF-2 or MSCs for large bone defects. METHODS:We studied an atrophic non-union model with a large ...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-016-0442-6
更新日期:2016-09-26 00:00:00
abstract:BACKGROUND:High tibial osteotomy (HTO) is a common treatment for moderate osteoarthritis of the medial compartment in the knee joint by the translation of the force center toward the lateral compartment. However, the stability of a short plate such as Puddu used in this procedure was not as effective as other long plat...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-019-1269-8
更新日期:2019-07-16 00:00:00
abstract:BACKGROUND:Mesenchymal stem cells (MSCs) have great potential for the repair and regeneration of bone fracture, but their optimal origins remain controversial. METHODS:Bone marrow-MSCs (BM-MSCs) and bone-bone marrow-MSCs (B-BM-MSCs) were isolated from 12 SD rats, and the morphology, MSC-associated markers, and prolife...
journal_title:Journal of orthopaedic surgery and research
pub_type: 杂志文章
doi:10.1186/s13018-019-1346-z
更新日期:2019-09-03 00:00:00