Normoxic wound fluid contains high levels of vascular endothelial growth factor.

Abstract:

OBJECTIVE:To examine the temporal integration of vascular endothelial growth factor (VEGF), which has been shown to be present in wound fluid, with the putatively related processes of wound fluid oxygen content, wound angiogenesis, and granulation tissue formation. SUMMARY BACKGROUND DATA:During cutaneous wound repair, new tissue formation starts with reepithelialization and is followed by granulation tissue formation, including neutrophil and macrophage accumulation, fibroblast ingrowth, matrix deposition, and angiogenesis. Because angiogenesis and increased vascular permeability are characteristic features of wound healing, VEGF may play an important role in tissue repair. METHODS:A ventral hernia, surgically created in the abdominal wall of female swine, was repaired using silicone sheeting and skin closure. Over time, a fluid-filled wound compartment formed, bounded by subcutaneous tissue and omentum. Ultrasonography was performed serially to examine the anatomy and dimensions of the subcutaneous tissue and wound compartment. Serial wound fluid samples, obtained by percutaneous aspiration, were analyzed for PO2, PCO2, pH, and growth factor concentrations. RESULTS:Three independent assays demonstrate that VEGF protein is present at substantially elevated levels in a wound fluid associated with the formation of abdominal granulation tissue. However, the wound fluid is not hypoxic at any time. Serial sampling reveals that transforming growth factor beta-1 protein appears in the wound fluid before VEGF. CONCLUSIONS:The results suggest that VEGF is a prominent regulator of wound angiogenesis and vessel permeability. A factor other than hypoxia, perhaps the earlier appearance of another growth factor, transforming growth factor beta-1, may positively regulate VEGF appearance in the wound fluid.

journal_name

Ann Surg

journal_title

Annals of surgery

authors

Howdieshell TR,Riegner C,Gupta V,Callaway D,Grembowicz K,Sathyanarayana,McNeil PL

doi

10.1097/00000658-199811000-00011

subject

Has Abstract

pub_date

1998-11-01 00:00:00

pages

707-15

issue

5

eissn

0003-4932

issn

1528-1140

journal_volume

228

pub_type

杂志文章
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    authors: Tukiainen E,Kallio M,Lepäntalo M

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    pub_type: 杂志文章

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    doi:10.1097/SLA.0000000000003768

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    更新日期:2020-01-03 00:00:00