Abstract:
:Multicentric chromosomes are often found in tumor cells and certain cell lines. How they are generated is not fully understood, though their stability suggests that they are non-functional during chromosome segregation. Growing evidence has implicated microtubule motor proteins in attachment of chromosomes to the mitotic spindle and in chromosome movement. To better understand the molecular basis for the inactivity of centromeres associated with secondary constrictions, we have tested these structures by immunofluorescence microscopy for the presence of motor complexes and associated proteins. We find strong immunoreactivity at the active, but not inactive, centromeres of prometaphase multicentric chromosomes using antibodies to the cytoplasmic dynein intermediate chains, three components of the dynactin complex (dynamitin, Arp1 and p150 Glued ), the kinesin-related proteins CENP-E and MCAK and the proposed structural and checkpoint proteins HZW10, CENP-F and Mad2p. These results offer new insight into the assembly and composition of both primary and secondary constrictions and provide a molecular basis for the apparent inactivity of the latter during chromosome segregation.
journal_name
Hum Mol Genetjournal_title
Human molecular geneticsauthors
Faulkner NE,Vig B,Echeverri CJ,Wordeman L,Vallee RBdoi
10.1093/hmg/7.4.671subject
Has Abstractpub_date
1998-04-01 00:00:00pages
671-7issue
4eissn
0964-6906issn
1460-2083pii
ddb079journal_volume
7pub_type
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