Oxidative downmodulation of the transient K-current IA by intracellular arachidonic acid in rat hippocampal neurons.

Abstract:

:Membrane-permeable arachidonic acid (AA) is liberated in a Ca2+-dependent way inside cells. By using whole cell patch clamp we show that intracellular AA (1 pM) selectively reduces IA in rat hippocampal neurons, whereas extracellular application requires a 10(6)-fold concentration. The nonmetabolized AA analogue ETYA mimics the effect of AA that is blocked by ascorbic acid or intracellular glutathione, suggesting an intracellular oxidative mechanism. We conclude that intracellular AA is extremely potent in reducing IA by an oxidative mechanism, particularly during oxidative stress.

journal_name

J Neurophysiol

authors

Bittner K,Müller W

doi

10.1152/jn.1999.82.1.508

subject

Has Abstract

pub_date

1999-07-01 00:00:00

pages

508-11

issue

1

eissn

0022-3077

issn

1522-1598

journal_volume

82

pub_type

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