Abstract:
:We have established an erythropoietin-dependent human leukemia cell line, AS-E2, from a patient with acute myeloid leukemia. These cells have many characteristics of late erythroid progenitor cells, they are positive for CD36, Glycophorin A, and CD71 but negative for CD41, and positive for benzidine and PAS staining. These cells express GATA-1 and have low affinity erythropoietin (EPO) receptor on their surface. Interestingly, AS-E2 cells are strictly dependent on EPO for their growth and survival; other cytokines including GM-CSF, stem cell factor, or IL-3 cannot support the growth of this cell line. These features are similar to late erythroid lineage cells, like normal BFU-E or CFU-E, and we have demonstrated that EPO stimulation induces the tyrosine phosphorylation of several proteins in AS-E2 cells including the EPO receptor and JAK2 kinase. This new cell line is a useful reagent to study biological and molecular events during the late stages of erythropoiesis, and to understand transforming events in human erythroid cells.
journal_name
Leukemiajournal_title
Leukemiaauthors
Miyazaki Y,Kuriyama K,Higuchi M,Tsushima H,Sohda H,Imai N,Saito M,Kondo T,Tomonaga Mdoi
10.1038/sj.leu.2400838subject
Has Abstractpub_date
1997-11-01 00:00:00pages
1941-9issue
11eissn
0887-6924issn
1476-5551journal_volume
11pub_type
杂志文章相关文献
LEUKEMIA文献大全abstract::c-MYC (hereafter MYC) overexpression has been recognized in aggressive B-cell lymphomas and linked to adverse prognosis. MYC activation results in widespread repression of micro-RNA (miRNA) expression and associated with lymphoma aggressive progression. Our recent study identified a MYC-miRNA-EZH2 feed-forward loop li...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2013.94
更新日期:2013-12-01 00:00:00
abstract::Neutropenia-related fungal infections can be life-threatening despite antifungal therapy. We evaluated the role of recombinant granulocyte colony-stimulating factor (rG-CSF)-elicited white blood cell (WBC) transfusions in patients with neutropenia-related fungal infections. Adult patients with hematologic malignancies...
journal_title:Leukemia
pub_type: 临床试验,杂志文章
doi:10.1038/sj.leu.2400811
更新日期:1997-10-01 00:00:00
abstract::A consecutive population-based series of 372 adult acute myeloid leukemias, successfully cytogenetically investigated at a single center between 1976 and 1993, is reported. All medical records were reviewed in order to ascertain the prognostic impact of karyotype, divided into three groups; favorable (t(8;21), t(15;17...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2401788
更新日期:2000-06-01 00:00:00
abstract::We analyzed nucleotide sequence and intraclonal diversity of the rearranged immunoglobulin heavy chain gene variable region (VH gene) of CD5+ and CD5- diffuse large B cell lymphoma (DLBCL) to clarify the cell origin of de novo CD5+ DLBCL. Ten cases of CD5+ DLBCL and 29 cases of CD5- DLBCL were analyzed. The frequencie...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2402031
更新日期:2001-03-01 00:00:00
abstract::A patient with large granular lymphocyte (LGL) expansion (T-gamma lymphocytosis), neutropenia and thrombocytopenia was studied longitudinally. Analysis of peripheral blood mononuclear cells (PBMC) demonstrated an unusual large proportion of CD3+ T-lymphocytes expressing a gamma delta T-cell receptor (TcR-gamma delta)....
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1992-05-01 00:00:00
abstract::The FMS-like tyrosine kinase-3 (FLT3) gene is the most commonly mutated gene in acute myeloid leukemia (AML), and patients carrying internal tandem duplication (ITD) mutations have a poor prognosis. Long-term inhibition of FLT3 activity in these patients has been elusive. To provide a more complete understanding of FL...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2016.284
更新日期:2017-03-01 00:00:00
abstract::Human cytomegalovirus (CMV) infections and relapse of disease remain major problems after allogeneic stem cell transplantation (allo-SCT), in particular in combination with CMV-negative donors or cordblood transplantations. Recent data suggest a paradoxical association between CMV reactivation after allo-SCT and reduc...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2012.374
更新日期:2013-06-01 00:00:00
abstract::In order to assess the proliferative capacity of leukemic subpopulations and to know whether it can be related to the stage of maturation, the expression of two surface antigens identifying distinct steps of leukocyte differentiation (CD15 and CD34) was studied by flow cytometry in correlation with DNA content in 16 c...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
abstract::Blockade of tumor necrosis factor (TNF)alpha by a soluble TNF receptor fusion protein (etanercept; Enbrel) improved in vitro hemopoiesis from the marrow of patients with myelodysplastic syndrome (MDS). Therefore, we enrolled 14 MDS patients (4 RA, 2 RARS, 6 RAEB, 2 CMML), 44-80 (median 60) years old, in a pilot trial....
journal_title:Leukemia
pub_type: 临床试验,杂志文章
doi:10.1038/sj.leu.2402356
更新日期:2002-02-01 00:00:00
abstract::The AML1/ETO fusion transcript can be detected by reverse transcription polymerase chain reaction (RT-PCR) in patients with t(8;21)-associated acute myeloid leukemia (AML) in long-term complete remission (CR). Quantitation of the amount of the fusion transcript during CR may therefore be more predictive of cure or rel...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2401128
更新日期:1998-09-01 00:00:00
abstract::We report here the case of a patient with refractory anemia with excess of blasts (RAEB) which evolved into RAEB in transformation. The standard Philadelphia (Ph) chromosome was found by cytogenetic study at diagnosis and during evolution. Southern blot analysis showed breakpoint cluster region (bcr) rearrangement as ...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1989-03-01 00:00:00
abstract::Chromosomal translocations involving the MYC oncogene are a hallmark of Burkitt lymphoma but they are only found in a varying frequency in mature Burkitt-type acute lymphoblastic leukemia (B-ALL). We have investigated samples of 56 sporadic Burkitt leukemia/lymphoma patients for the translocations t(8;14)(q24;q32), t(...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2403847
更新日期:2005-08-01 00:00:00
abstract::Gain of chromosome 18q and translocation t(14;18) are] frequently found in B-cell non-Hodgkin's lymphomas (B-NHL). Increased BCL2 transcription and BCL2 protein expression have been suggested to be the result of the gain. We utilized FISH, PCR and array CGH to study BCL2 and chromosome 18 copy number changes and rearr...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2403954
更新日期:2005-12-01 00:00:00
abstract::Inhibition of NOTCH1 signaling with gamma-secretase inhibitors (GSIs) has been proposed as a molecularly targeted therapy in T-cell acute lymphoblastic leukemia (T-ALL). However, GSIs seem to have limited antileukemic activity in human T-ALL and are associated with severe gastrointestinal toxicity resulting from inhib...
journal_title:Leukemia
pub_type: 杂志文章,评审
doi:10.1038/leu.2009.75
更新日期:2009-08-01 00:00:00
abstract::Most modern treatment protocols for acute lymphoblastic leukaemia (ALL) include the analysis of minimal residual disease (MRD). To ensure comparable MRD results between different MRD-polymerase chain reaction (PCR) laboratories, standardization and quality control are essential. The European Study Group on MRD detecti...
journal_title:Leukemia
pub_type: 杂志文章,多中心研究
doi:10.1038/sj.leu.2404586
更新日期:2007-04-01 00:00:00
abstract::Monitoring of BCR-ABL transcripts has become established practice in the management of chronic myeloid leukemia. However, nucleic acid amplification techniques are prone to variations which limit the reliability of real-time quantitative PCR (RQ-PCR) for clinical decision making, highlighting the need for standardizat...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2404716
更新日期:2007-07-01 00:00:00
abstract::The mechanisms by which non-oncogene bearing, slowly transforming T-cell-tropic retroviruses induce leukemia is not well understood. Viruses such as the murine radiation leukemia virus (RadLV) induce oncogenic transformation of T-cells in the thymus only in vivo and after a long latency. The capacity of RadLV to induc...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1992-04-01 00:00:00
abstract::In order to improve leukemia-free survival (LFS) without the treatment-related morbidity of allogeneic bone marrow transplantation or multiple prolonged cycles of consolidation chemotherapy, we evaluated the long-term outcome of autologous transplantation of peripheral blood progenitor cells (PBPCs) as postremission t...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2403126
更新日期:2003-11-01 00:00:00
abstract::The nuclear export receptor, Exportin 1 (XPO1), mediates transport of growth-regulatory proteins, including tumor suppressors, and is overactive in many cancers, including chronic lymphocytic leukemia (CLL), acute myeloid leukemia (AML) and aggressive lymphomas. Oral selective inhibitor of nuclear export (SINE) compou...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2016.136
更新日期:2016-12-01 00:00:00
abstract::Small-molecule multi-targeted cyclin-dependent kinase (CDK) inhibitors (CDKIs) are of particular interest due to their potent antitumor activity independent of p53 gene alterations. P53 deletion is associated with a very poor prognosis in multiple myeloma (MM). In this regard, we tested the anti-MM activity of RGB-286...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2013.194
更新日期:2013-12-01 00:00:00
abstract::Immunoglobulin and T cell receptor gene rearrangement and expression were investigated within B cell acute lymphoblastic leukemias (ALLs) that are thought to be representative of the early stages of normal human B cell development. Although the immunoglobulin heavy chain locus is rearranged in leukemic cells from all ...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1989-11-01 00:00:00
abstract::Recently, it has been clarified that interaction between hematopoietic cells and endothelial cells is important in normal hematopoiesis and leukemogenesis. In this study, we examined the relationship between AML cells and endothelial cells by analyzing the expression profile of angiogenic factors, angiopoietin-1 (Ang-...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2402326
更新日期:2002-01-01 00:00:00
abstract::G-CSF-induced myeloid differentiation of 32Dcl3 murine myeloblast cells is antagonized by concurrent exposure to interleukin-3 (IL-3) or by oncogenic transformation of 32Dcl3 by src- or abl-oncogenes which render the cells IL-3-independent. Recent reports have linked G-CSF-mediated differentiation to the ability of G-...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2401253
更新日期:1999-01-01 00:00:00
abstract::Neomorphic mutations in isocitrate dehydrogenase 1 (IDH1) are frequently found in several human cancer types including acute myeloid leukemia (AML) and lead to the production of high levels of the oncometabolite (R)-2-hydroxyglutarate (R-2HG). Here we report the characterization of BAY1436032, a novel pan-mutant IDH1 ...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2017.46
更新日期:2017-10-01 00:00:00
abstract::In cell and animal models, telomere erosion promotes chromosomal instability via breakage-fusion-bridge cycles, contributing to the early stages of tumorigenesis. However, evidence involving short telomeres in cancer development in humans is scarce, epidemiological and indirect. Here we directly implicate telomere sho...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2011.272
更新日期:2012-04-01 00:00:00
abstract::Cytogenetic and fluorescence in situ hybridization studies have shown the presence of telomeric repeats in translocation present in three patients with hematopoietic malignancies. One had jumping translocations, involving 1q12 and 2q, 16p, and 19q. These sequences were detected by FISH only in derivative chromosomes t...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2401876
更新日期:2000-09-01 00:00:00
abstract::The scientific community faces an unexpected and urgent challenge related to the SARS-CoV-2 pandemic and is investigating the role of receptors involved in entry of this virus into cells as well as pathomechanisms leading to a cytokine "storm," which in many cases ends in severe acute respiratory syndrome, fulminant m...
journal_title:Leukemia
pub_type: 杂志文章,评审
doi:10.1038/s41375-020-0887-9
更新日期:2020-07-01 00:00:00
abstract::Twenty new cases of acute lymphoblastic leukemia (ALL) with the dicentric chromosome dic(9;20)(p1113;q11) are presented. This chromosomal abnormality is difficult to identify from G-banding alone. It masquerades as monosomy 20 and is only accurately identified by fluorescence in situ hybridization (FISH). Monosomy 20 ...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2401654
更新日期:2000-02-01 00:00:00
abstract::Novel approaches have been designed to treat leukemia based on our understanding of the genetic and biochemical lesions present in different malignancies. This meeting report summarizes some of the recent advances in leukemia treatment. Based on the discoveries of cellular oncogenes, chromosomal translocations, monocl...
journal_title:Leukemia
pub_type:
doi:10.1038/sj.leu.2403156
更新日期:2003-12-01 00:00:00
abstract::Bryostatin 1 is a macrocyclic lactone activator of protein kinase C which has displayed promising antileukemic potential in pre-clinical studies. We have assessed the effect of bryostatin 1 on the in vitro clonogenic response of leukemic myeloblasts obtained from 12 patients with acute non-lymphocytic leukemia to reco...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1991-05-01 00:00:00