Abstract:
:Monitoring of BCR-ABL transcripts has become established practice in the management of chronic myeloid leukemia. However, nucleic acid amplification techniques are prone to variations which limit the reliability of real-time quantitative PCR (RQ-PCR) for clinical decision making, highlighting the need for standardization of assays and reporting of minimal residual disease (MRD) data. We evaluated a lyophilized preparation of a leukemic cell line (K562) as a potential quality control reagent. This was found to be relatively stable, yielding comparable respective levels of ABL, GUS and BCR-ABL transcripts as determined by RQ-PCR before and after accelerated degradation experiments as well as following 5 years storage at -20 degrees C. Vials of freeze-dried cells were sent at ambient temperature to 22 laboratories on four continents, with RQ-PCR analyses detecting BCR-ABL transcripts at levels comparable to those observed in primary patient samples. Our results suggest that freeze-dried cells can be used as quality control reagents with a range of analytical instrumentations and could enable the development of urgently needed international standards simulating clinically relevant levels of MRD.
journal_name
Leukemiajournal_title
Leukemiaauthors
Saldanha J,Silvy M,Beaufils N,Arlinghaus R,Barbany G,Branford S,Cayuela JM,Cazzaniga G,Gonzalez M,Grimwade D,Kairisto V,Miyamura K,Lawler M,Lion T,Macintyre E,Mahon FX,Muller MC,Ostergaard M,Pfeifer H,Saglio G,Sawdoi
10.1038/sj.leu.2404716subject
Has Abstractpub_date
2007-07-01 00:00:00pages
1481-7issue
7eissn
0887-6924issn
1476-5551pii
2404716journal_volume
21pub_type
杂志文章相关文献
LEUKEMIA文献大全abstract::I use the hematological, morphological and molecular criteria recently established by the World Health Organization to diagnose essential thrombocytemia. In these patients, major causes of morbidity and mortality are represented by thrombosis and bleeding, whereas progression to myelofibrosis and transformation to acu...
journal_title:Leukemia
pub_type: 杂志文章,评审
doi:10.1038/leu.2011.306
更新日期:2012-05-01 00:00:00
abstract::In order to assess the proliferative capacity of leukemic subpopulations and to know whether it can be related to the stage of maturation, the expression of two surface antigens identifying distinct steps of leukocyte differentiation (CD15 and CD34) was studied by flow cytometry in correlation with DNA content in 16 c...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
abstract::In this study, fluorescence in situ hybridization (ISH) with an alphoid probe was used for the detection of trisomy 8 on archival blood smears (BS). The results were compared with hybridization experiments performed on methanol/acetic acid fixed cells of cytogenetic preparations (CP) which are widely used for ISH. Fiv...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1993-05-01 00:00:00
abstract::Cord blood (CB) is an attractive alternative to bone marrow or peripheral blood as a source of transplantable hematopoietic tissue. However, because of the reduced volume, the stem cell content is limited; therefore its use as a graft for adult patients might require ex vivo manipulations. Two systems have been descri...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2401003
更新日期:1998-05-01 00:00:00
abstract::This report documents the follow-up information on 69 hairy cell leukemia (HCL) patients treated with interferon alpha-2b (IFN) as primary treatment from 1983-86. Follow-up through October 1991 shows only 11 patients have died. Forty-one of the 57 patients completing the intended 12 or more months of initial IFN treat...
journal_title:Leukemia
pub_type: 临床试验,杂志文章
doi:
更新日期:1992-11-01 00:00:00
abstract::The T-lineage phenotype in children with acute lymphoblastic leukaemia (ALL) is associated with in vitro drug resistance and a higher relapse-risk compared to a precursor B phenotype. Our study was aimed to investigate whether mutations in the ATM gene occur in childhood T-lineage acute lymphoblastic leukaemia (T-ALL)...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2403943
更新日期:2005-11-01 00:00:00
abstract::The proto-oncogene c-Jun has been implicated in the control of cell proliferation and differentiation and more recently in the regulation of apoptosis. We have previously reported the involvement of c-Jun in the erythroid differentiation block in murine erythroleukemia (MEL) cells. As reported here, we investigated th...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2402350
更新日期:2002-02-01 00:00:00
abstract::Fludarabine combination chemotherapy achieves high response rates in chronic lymphocytic leukemia (CLL) and indolent lymphoma. The aim of this study was to investigate the incidence and characteristics of treatment-related myelodysplasia and acute myeloid leukemia (t-MDS/AML) after treatment with fludarabine in combin...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2010.218
更新日期:2010-12-01 00:00:00
abstract::The expansion of the Philadelphia (Ph) chromosome positive clone in chronic myeloid leukemia (CML) may depend on its capacity to suppress the proliferation of Ph-negative stem cells, but this proliferative advantage might, in certain circumstances, be reversible. Various lines of evidence suggest that Ph-negative cell...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1991-06-01 00:00:00
abstract::CD31/PECAM-1 (platelet endothelial cell adhesion molecule-1) is a 130 kDa integral membrane protein of the immunoglobulin gene superfamily with the distinctive feature of being expressed on several cell types associated with the vascular compartment. In the present study we report a novel, unique CD31 mAb termed IP28A...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1996-08-01 00:00:00
abstract::We looked for bcl-2 protein expression by immunocytochemistry on bone marrow slides from 51 cases of myelodysplastic syndrome (MDS), of whom 25 received some form of chemotherapy. Forty-six of them had at least 20% bcl-2 positive blasts and the median percentage of positive blasts was 80%, whereas myeloid cells beyond...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1995-04-01 00:00:00
abstract::Older adults with acute myeloid leukemia (AML) who are not fit for standard chemotherapy historically have poor outcomes. Approximately 12-15% of older patients with AML harbor isocitrate dehydrogenase 2 (IDH2) gene mutations. Enasidenib is an oral inhibitor of mutant IDH2 proteins. Among 39 patients with newly diagno...
journal_title:Leukemia
pub_type: 杂志文章,多中心研究
doi:10.1038/s41375-019-0472-2
更新日期:2019-11-01 00:00:00
abstract::Improved survival of patients with acute lymphoblastic leukemia (ALL) has emerged from identifying new prognostic markers; however, 20% of children still suffer recurrence. Previously, the altered expression of Fat1 cadherin has been implicated in a number of solid tumors. In this report, in vitro analysis shows that ...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2011.319
更新日期:2012-05-01 00:00:00
abstract::MUM1 (multiple myeloma oncogene 1)/IRF4 (interferon regulatory factor 4) is a transcription factor that is activated as a result of t(6;14)(p25;q32) in multiple myeloma. MUM1 expression is seen in various B-cell lymphomas and predicts an unfavorable outcome in some lymphoma subtypes. To elucidate its role in B-cell ma...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2403833
更新日期:2005-08-01 00:00:00
abstract::Recent evidence has established an important role for leukemia inhibitory factor (LIF) as hematopoietically active cytokine. The present study utilized two different murine bone marrow stromal cell lines, +/+-1.LDA11 and MBA-13, to define regulatory mechanisms of LIF messenger RNA (mRNA) induction. LIF mRNA was not de...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1993-04-01 00:00:00
abstract::A human acute lymphoblastic leukemia (ALL) cell line, BALM-9, was established from the peripheral blood specimen of a immunoglobin (lg) phenotype, the established BALM-9 cell line expressed both kappa and lambda light (L) chains simultaneously in a range of 30-80%. Two-color flow cytometric analysis demonstrated that ...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1996-04-01 00:00:00
abstract::B-cell acute lymphoblastic leukemia (B-ALL) is often associated with chromosomal translocations leading to the deregulation of proto-oncogenes. MicroRNAs can also be affected by chromosomal alterations and thus contribute to carcinogenesis. The microRNA, miR-125b-1, is overexpressed in B-ALL cases with the t(11;14)(q2...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2012.95
更新日期:2012-10-01 00:00:00
abstract::Chronic myeloid leukemia (CML) relapse after allogeneic stem cell transplantation (SCT) is a relatively frequent situation, which is correlated to disease status, time from diagnosis to transplant and T-cell depletion. We evaluated the potential for early minimal residual disease (MRD) BCR-ABL quantification to predic...
journal_title:Leukemia
pub_type: 临床试验,杂志文章
doi:10.1038/sj.leu.2404170
更新日期:2006-05-01 00:00:00
abstract::Schedule dependency of idarubicin (Ida) and doxorubicin (Dox) toxicity was investigated in vitro using the K562 human leukemia cell line. For Dox, repeated exposure to the IC30 (d x 3) resulted in comparable survival as single exposure to the total accumulative dose (20%). For Ida, repeated exposure to the IC30 (d x 3...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1994-08-01 00:00:00
abstract::Most modern treatment protocols for acute lymphoblastic leukaemia (ALL) include the analysis of minimal residual disease (MRD). To ensure comparable MRD results between different MRD-polymerase chain reaction (PCR) laboratories, standardization and quality control are essential. The European Study Group on MRD detecti...
journal_title:Leukemia
pub_type: 杂志文章,多中心研究
doi:10.1038/sj.leu.2404586
更新日期:2007-04-01 00:00:00
abstract::Expression of the human multidrug resistance 1 gene (MDR1), which encodes the P-glycoprotein transmembrane efflux pump, has been associated with treatment failure of some leukemias, primarily acute myeloid leukemia (AML). To elucidate the epigenetic events associated with overexpression of MDR1 in AML, we analyzed the...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2401424
更新日期:1999-06-01 00:00:00
abstract::Graft-versus-host disease (GVHD) remains a major immunological complication after allogeneic bone marrow transplantation (allo-BMT), but also favors development of the beneficial graft-versus-leukemia (GVL) effect. A patient with AML-M4 (inv (16)) is described, who was given non-myeloablative remission reinduction the...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2400810
更新日期:1997-10-01 00:00:00
abstract::HTLV-1 is an important factor involved in various diseases including adult T-cell leukemia/lymphoma and HTLV-1 associated myelopathy/tropical spastic paraparesis. Amount of HTLV-1 provirus integrated in human peripheral blood mononuclear cells might be a candidate for a risk factor in the manifestation of HTLV-1 assoc...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1997-04-01 00:00:00
abstract::This study has determined the effects of phorbol-12-myristate-13-acetate (PMA) and dimethylsulfoxide (DMSO) on mRNA levels for the serglycin proteoglycan core protein in human erythroleukemia (HEL) cells. We have compared these changes to those for mRNA for other proteins which are known to be synthesized by HEL cells...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1993-12-01 00:00:00
abstract::The hematopoietic system is sustained by a rare population of hematopoietic stem cells (HSCs), which emerge during early embryonic development and then reside in the hypoxic niche of the adult bone marrow microenvironment. Although leptin receptor (Lepr)-expressing stromal cells are well-studied as critical regulators...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/s41375-020-01079-z
更新日期:2020-11-07 00:00:00
abstract::In patients with hematologic malignancy, invasive aspergillosis continues to be associated with high mortality even when treated with conventional antifungal therapy. To investigate novel antifungal agents, we compared 53 patients who received posaconazole salvage therapy to 52 contemporary control patients who receiv...
journal_title:Leukemia
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1038/sj.leu.2405065
更新日期:2008-03-01 00:00:00
abstract::Previous studies in pediatric patients with acute myelogenous leukemia (AML) have suggested that 2-chlorodeoxyadenosine (2CdA) is an effective therapeutic agent. Santana et al (J Clin Oncol 1992; 10: 364-370) reported a CR rate of 8/17 (95% Cl 23-72%) in children with relapsed AML and a median first CR of 21 months. T...
journal_title:Leukemia
pub_type: 临床试验,杂志文章
doi:
更新日期:1996-10-01 00:00:00
abstract::The hematopoietic growth factors are glycoproteins that can be produced by recombinant DNA technology. They have many potential clinical uses in acute leukemia; several areas have been explored extensively and much data are available from clinical trials. Other areas are of potential interest, but have a paucity of cl...
journal_title:Leukemia
pub_type: 杂志文章,评审
doi:10.1038/sj.leu.2400592
更新日期:1997-03-01 00:00:00
abstract::To characterise the genetics of splenic marginal zone lymphoma (SMZL), we performed whole exome sequencing of 16 cases and identified novel recurrent inactivating mutations in Kruppel-like factor 2 (KLF2), a gene whose deficiency was previously shown to cause splenic marginal zone hyperplasia in mice. KLF2 mutation wa...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2014.330
更新日期:2015-05-01 00:00:00
abstract::T-cell large granular lymphocytic (LGL) leukemia is a complex diagnosis, requiring persistent clonal expansions of LGLs, and cytopenias. Often the diagnosis is unclear as non-clonal expansions of LGLs commonly occur in reactive conditions. To better understand T-LGL leukemia, we performed a comprehensive clinicopathol...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2011.107
更新日期:2011-09-01 00:00:00