Abstract:
:We studied the differentiation profiles of B cell precursors (BCP) in normal and post-chemotherapy pediatric bone marrow (BM) using multiparameter flow cytometry. The goal of our study was to draw a comprehensive phenotypic map of the three major maturational BCP stages in BM. By correlating lineage-associated markers, CD45RA, and several adhesion molecules, the stage-specific patterns were found to differ in certain details from previously published concepts. Among the earliest BCP, a subset of CD34+ CD10(lo) precursors was repeatedly observed in addition to the well characterized CD34+ CD10(hi) CD19+ majority of cells. Only two-thirds of these CD34+ CD10(lo) cells expressed CD19. However, uniformity of phenotypic features, absence of T lineage markers, and the regeneration kinetics after chemotherapy suggest the B lineage affiliation of the CD34+ CD10(lo) precursors in general. In the more mature BCP, expression of CD10, CD20, cytoplasmic and surface mu chains (c mu and s mu) was observed to overlap more than previously recognized. We found that CD20 and c mu appear early during B cell ontogeny (already on CD34+ BCP), and that CD10 is lost late, following the onset of s mu expression. Differences between normal and post-chemotherapy BM specimens regarding the phenotypic appearance of BCP were exclusively due to differences in the subset composition, as post-chemotherapy samples showed a preponderance of immature stages. Our observations may build a framework for comparing leukemic cells with their normal counterparts to define possible leukemia-associated aberrations useful for residual disease studies.
journal_name
Leukemiajournal_title
Leukemiaauthors
Dworzak MN,Fritsch G,Fleischer C,Printz D,Fröschl G,Buchinger P,Mann G,Gadner Hdoi
10.1038/sj.leu.2400732subject
Has Abstractpub_date
1997-08-01 00:00:00pages
1266-73issue
8eissn
0887-6924issn
1476-5551journal_volume
11pub_type
杂志文章相关文献
LEUKEMIA文献大全abstract::The unbalanced translocation, der(1;7)(q10;p10), is one of the characteristic cytogenetic abnormalities found in myelodysplastic syndromes (MDS) and other myeloid neoplasms. Although described frequently with very poor clinical outcome and possible relationship with monosomy 7 or 7q- (-7/7q-), this recurrent cytogenet...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2404619
更新日期:2007-05-01 00:00:00
abstract::It has been proposed that adoptive immunotherapy, for the treatment of relapsed AML, with cytotoxic T lymphocytes which show a relative specificity for the leukemic cells may have the advantage of maximizing the beneficial anti-leukemic effect whilst minimizing the probability of graft-versus-host disease. In this stu...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2402013
更新日期:2001-02-01 00:00:00
abstract::This study was undertaken to determine the efficacy and toxicity of a shorter schedule of fludarabine administration (30 mg/m2 i.v. daily for 3 days every 4 weeks) in patients with previously treated chronic lymphocytic leukemia (CLL). Eighty patients with previously treated advanced (Rai III-IV) (54%) or progressive ...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1995-09-01 00:00:00
abstract::Proto-oncogenes are thought to be involved in cellular differentiation and proliferation. Tumor necrosis factors (TNFs) are specific cytokines that have cytostatic and cytotoxic effects in vitro against a wide range of human tumor cells. We have previously demonstrated that recombinant TNFs (rTNFs) have an antiprolife...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1988-11-01 00:00:00
abstract::The fusion transcript AML1/ETO corresponding to translocation t(8;21)(q22;q22) can be found in approximately 7-12% of childhood de novo AML. Despite the favorable prognosis, some of these patients relapse. Most of MRD studies so far were performed on adults treated not uniformly. Therefore, we analyzed the follow-up o...
journal_title:Leukemia
pub_type: 杂志文章,多中心研究
doi:10.1038/sj.leu.2402959
更新日期:2003-06-01 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Leukemia
pub_type: 已发布勘误
doi:10.1038/s41375-020-0821-1
更新日期:2020-10-01 00:00:00
abstract::Multiple myeloma (MM) is characterized by bone marrow infiltration with abnormal plasma cells which synthesize monoclonal immunoglobulins (Ig) or Ig fragments. Regularly, MM cells exhibit a high intrinsic resistance to available chemotherapeutic strategies. A number of cellular alterations including the cellular membr...
journal_title:Leukemia
pub_type: 杂志文章,评审
doi:
更新日期:1997-12-01 00:00:00
abstract::Evidence is presented that human monocytes and acute myeloblastic leukemic (AML) cells contain both high and low affinity binding sites for interleukin-4 (IL-4). On monocytes 183 +/- 132 high affinity binding sites per cell with a Kd of 60 +/- 29 pM and 1500 +/- 600 low affinity receptors with a Kd of 2.3 +/- 0.4 nM (...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1991-09-01 00:00:00
abstract::Nodular lymphocyte predominant Hodgkin lymphoma (NLPHL)-a subtype of Hodgkin lymphoma (HL)-is characterized by a low content of tumor cells, the lymphocyte predominant (LP) cells. Transformation into diffuse large B-cell lymphoma (DLBCL) occurs in about 10% of patients. We performed whole-genome mutation analysis of t...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2015.328
更新日期:2016-04-01 00:00:00
abstract::Smoldering multiple myeloma (SMM) is a biologically heterogeneous, clinically defined entity with a variable rate of progression to symptomatic multiple myeloma (MM). Reliable markers for progression are critical for the development of potential therapeutic interventions. We retrospectively evaluated the predictive va...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/s41375-018-0013-4
更新日期:2018-06-01 00:00:00
abstract::Bortezomib is a potent drug for the treatment of multiple myeloma. Its anti-tumor activity is mediated by proteasome inhibition leading to decreased cell proliferation and induction of apoptosis. However, an unimpaired proteasomal function plays a crucial role for the induction of anti-tumor immunity by dendritic cell...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2404734
更新日期:2007-07-01 00:00:00
abstract::During T cell selection in the thymic cortex more than 90% of the thymocytes are eliminated by apoptosis. Based on this biology, we propose to define blasts of T cell acute lymphoblastic leukemia (ALL) with the phenotype of cortical thymocytes (CD1+ and/or CD4+ 8+) as selection-related (SR) and all other T-ALL immunop...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2401382
更新日期:1999-04-01 00:00:00
abstract::Upregulation of the immunosuppressive cell surface glycoprotein, CD200, is a common feature of acute myeloid leukemia (AML) and is associated with poor patient outcome. We investigated whether CD200 overexpression on AML cells could specifically compromise patient natural killer (NK) cell anti-tumor responses. We foun...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2011.1
更新日期:2011-05-01 00:00:00
abstract::Eventually, gene therapy may be a valid option for chronic viral infections, including retroviral infections. Human retroviral diseases fit two categories: (1) those that result from a monoclonal outgrowth of a human T-cell leukemia virus type I (HTLV-I)-infected cell, as in the case of adult T cell leukemia (ATL); an...
journal_title:Leukemia
pub_type: 杂志文章,评审
doi:
更新日期:1995-10-01 00:00:00
abstract::Successful treatment results for MLL-rearranged Acute Lymphoblastic Leukemia (ALL) in infants remain difficult to achieve. Significantly contributing to therapy failure is poor response to glucocorticoids (GCs), like prednisone. Thus, overcoming resistance to these drugs may be a crucial step towards improving prognos...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2013.245
更新日期:2014-04-01 00:00:00
abstract::The AML1/ETO fusion transcript can be detected by reverse transcription polymerase chain reaction (RT-PCR) in patients with t(8;21)-associated acute myeloid leukemia (AML) in long-term complete remission (CR). Quantitation of the amount of the fusion transcript during CR may therefore be more predictive of cure or rel...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2401128
更新日期:1998-09-01 00:00:00
abstract::The acquired kinase mutation JAK2V617F plays a central role in myeloproliferative neoplasms (MPNs). However, the mechanisms responsible for the malignant hematopoietic stem/progenitor cell (HSPC) expansion seen in patients with MPNs are not fully understood, limiting the effectiveness of current treatment. Endothelial...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2017.233
更新日期:2018-02-01 00:00:00
abstract::This multicentre, open-label phase 1/2 trial determined safety and efficacy of weekly carfilzomib plus cyclophosphamide-dexamethasone (wKCyd) in newly diagnosed multiple myeloma (NDMM) patients aged ⩾65 years or transplant ineligible. Patients received wKCyd for up to nine 28-day cycles, followed by maintenance with c...
journal_title:Leukemia
pub_type: 杂志文章,多中心研究
doi:10.1038/leu.2017.327
更新日期:2018-04-01 00:00:00
abstract::14-3-3 proteins are a family of master regulators of intracellular signaling, yet their impact on proteasome function is unknown. We demonstrate that 14-3-3ζ binds the 11S proteasome activator, limiting proteasome assembly and cellular capacity for protein degradation. To define the functional impact of 14-3-3ζ protea...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2017.288
更新日期:2018-03-01 00:00:00
abstract::The myeloproliferative neoplasms (MPNs) are characterized by hematopoietic stem/progenitor cell (HSPC) expansion and overproduction of mature blood cells. The JAK2V617F mutation is present in hematopoietic cells in a majority of patients with MPNs, but the mechanism(s) responsible for MPN stem cell expansion remain in...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2016.114
更新日期:2016-12-01 00:00:00
abstract::Runt-related transcription factor 1 (RUNX1) is essential for normal hematopoiesis. RUNX1 mutations have rarely been reported in chronic myelomonocytic leukemia (CMML). We examined RUNX1 mutations in 81 patients with CMML at initial diagnosis. Mutational analysis was performed on bone marrow samples by direct sequencin...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2009.48
更新日期:2009-08-01 00:00:00
abstract::With a seroprevalence rate (SPR) of 6%-10% among healthy adult blood donors (ABD), Nigeria and other African locales represent an endemic zone for HTLV-I. We studied SPR in patients with leukaemia, lymphomas, solid tumours, and chronic disorders, as well as in groups of men and women with varying sexual lifestyles. Se...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1994-04-01 00:00:00
abstract::Translocation (12;21), the most frequent chromosomal aberration in childhood acute lymphoblastic leukemia, creates TEL/AML1 fusion gene. Resulting hybrid protein was shown to have a role in pre-leukemia establishment. To address its role for leukemic cell survival, we applied RNA interference to silence TEL/AML1 in le...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2010.277
更新日期:2011-02-01 00:00:00
abstract::Improved survival of patients with acute lymphoblastic leukemia (ALL) has emerged from identifying new prognostic markers; however, 20% of children still suffer recurrence. Previously, the altered expression of Fat1 cadherin has been implicated in a number of solid tumors. In this report, in vitro analysis shows that ...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2011.319
更新日期:2012-05-01 00:00:00
abstract::Previous studies demonstrated that triazene compounds (TZC) possess antitumor, antimetastatic and immunosuppressive activity, and induce novel antigenic properties in neoplastic cells. Moreover, TZC showed marked antitumor activity in patients with acute myelogenous leukemias (AML). In most cases leukemic blasts with ...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1995-11-01 00:00:00
abstract::The EU-supported EuroFlow Consortium aimed at innovation and standardization of immunophenotyping for diagnosis and classification of hematological malignancies by introducing 8-color flow cytometry with fully standardized laboratory procedures and antibody panels in order to achieve maximally comparable results among...
journal_title:Leukemia
pub_type: 杂志文章,评审
doi:10.1038/leu.2012.122
更新日期:2012-09-01 00:00:00
abstract::Hematopoietic stem/progenitor cells (HSC/P) reside in the bone marrow in distinct anatomic locations (niches) to receive growth, survival and differentiation signals. HSC/P localization and migration between niches depend on cell-cell and cell-matrix interactions, which result from the cooperation of cytokines, chemok...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/sj.leu.2404769
更新日期:2007-08-01 00:00:00
abstract::Children with Down's syndrome (DS) have an increased risk of developing acute lymphoblastic leukemia (ALL) and have a low frequency of established genetic aberrations. We aimed to determine which genetic abnormalities are involved in DS ALL. We studied the frequency and prognostic value of deletions in B-cell developm...
journal_title:Leukemia
pub_type: 杂志文章
doi:10.1038/leu.2012.84
更新日期:2012-10-01 00:00:00
abstract::Different normal and malignant human B-cell populations were studied with a twofold aim: to define which cytokines are produced in vivo, and to assess the relationship between cytokine production and kinetic state. To analyse normal B-cells representative of different stages of activation and proliferation in vivo, we...
journal_title:Leukemia
pub_type: 杂志文章
doi:
更新日期:1992-02-01 00:00:00
abstract::Remarkable progress has been achieved in the characterization and isolation of primitive hematopoietic stem cells (HSC). HSC represent a very small subset of hematopoietic cells and provide self-renewal, possess differentiation capacity and allow a constant supply of the entire hematopoietic cell spectrum. Until recen...
journal_title:Leukemia
pub_type: 杂志文章,评审
doi:10.1038/sj.leu.2402620
更新日期:2002-09-01 00:00:00