Abstract:
:A comparison between the effects of schisandrin B (Sch B) and butylated hydroxytoluene (BHT) treatments on hepatic antioxidant status was made to identify the critical antioxidant action of Sch B involved in hepatoprotection in mice. Whereas Sch B treatment (3 mmol/kg/day x 3, p.o.) increased the hepatic mitochondrial-reduced glutathione (GSH) level, BHT treatment at the same dosage regimen decreased it. However, both Sch B and BHT increased, albeit to a different extent, the activity of mitochondrial glutathione reductase. The differential effect of Sch B and BHT treatment on hepatic mitochondrial glutathione status became more apparent after carbon tetrachloride (CCl4) challenge. Pretreatment with Sch B could sustain the hepatic mitochondrial GSH level in CCl4-intoxicated mice and protect against CCl4 hepatotoxicity. BHT pretreatment did not produce any protective effect on CCl4-induced GSH depletion in mitochondrion and hepatocellular damage. Although both Sch B and BHT treatments increased hepatic ascorbic acid (VC) level in control animals, only Sch B pretreatment sustained a high hepatic VC level in CCl4-intoxicated mice. Moreover, Sch B pretreatment prevented the CCl4-induced decrease in the hepatic alpha-tocopherol (VE) level. However, Sch B inhibited NADPH oxidation in mouse liver microsomes incubated with CCl4 in vitro, whereas BHT stimulated this oxidation. The ensemble of results suggests that the ability to sustain the hepatic mitochondrial GSH level and the hepatic VC and VE levels may represent the crucial antioxidant action of Sch B in protection against CCl4 hepatotoxicity. The possible inhibition of CCl4 metabolism by Sch B may also contribute to its hepatoprotective action.
journal_name
Biochem Pharmacoljournal_title
Biochemical pharmacologyauthors
Ip SP,Ko KMdoi
10.1016/s0006-2952(96)00517-5subject
Has Abstractpub_date
1996-12-13 00:00:00pages
1687-93issue
11eissn
0006-2952issn
1873-2968pii
S0006-2952(96)00517-5journal_volume
52pub_type
杂志文章abstract::Most of the cytochrome P450 (CYP) genes are expressed in an uneven, zonated pattern in the liver. Factors regulating this regionally restricted expression are not well known. In this study we have analysed cell lysates obtained from opposite zones of rat liver by infusing digitonin to the perfused liver to study the z...
journal_title:Biochemical pharmacology
pub_type: 杂志文章
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
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更新日期:1991-08-08 00:00:00
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pub_type: 杂志文章
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1996-07-12 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1999-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2008-04-01 00:00:00
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更新日期:1998-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-08-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章,评审
doi:10.1016/j.bcp.2006.07.010
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
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更新日期:1987-10-15 00:00:00
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pub_type: 杂志文章
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更新日期:2007-10-15 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2005-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2012-12-01 00:00:00
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
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更新日期:1992-08-18 00:00:00
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
doi:10.1016/s0006-2952(96)00869-6
更新日期:1997-06-01 00:00:00
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journal_title:Biochemical pharmacology
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:1999-04-15 00:00:00
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pub_type: 杂志文章
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更新日期:1986-12-01 00:00:00