Heterogeneity of carboxylesterases in rat liver cells.

Abstract:

:Rat liver cells were separated into parenchymal cells (PC), Kupffer cells (KC) and endothelial cells (EC). The distribution of carboxylesterases (EC 3.1.1.1) between these cell types was investigated by PAGE and chromatogenic substrate staining, and compared with the results for total liver preparation and individual isoenzymes isolated by chromatofocusing. All of the liver carboxylesterase isoenzymes could be detected in the PC, whereas in both KC and EC only those with isoelectric point (pI) 6.4/6.2 could be detected. Use of carboxylesterase inhibitors like bis-(4-nitrophenyl)phosphate and paraoxon, and organophosphorus compound hydrolase inhibitors like 4-hydroxymercuribenzoate and EDTA confirmed that these esterases were of the carboxylesterase type.

journal_name

Biochem Pharmacol

journal_title

Biochemical pharmacology

authors

Gaustad R,Berg T,Fonnum F

doi

10.1016/0006-2952(92)90423-g

subject

Has Abstract

pub_date

1992-08-18 00:00:00

pages

827-9

issue

4

eissn

0006-2952

issn

1873-2968

pii

0006-2952(92)90423-G

journal_volume

44

pub_type

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