Abstract:
:Rat liver cells were separated into parenchymal cells (PC), Kupffer cells (KC) and endothelial cells (EC). The distribution of carboxylesterases (EC 3.1.1.1) between these cell types was investigated by PAGE and chromatogenic substrate staining, and compared with the results for total liver preparation and individual isoenzymes isolated by chromatofocusing. All of the liver carboxylesterase isoenzymes could be detected in the PC, whereas in both KC and EC only those with isoelectric point (pI) 6.4/6.2 could be detected. Use of carboxylesterase inhibitors like bis-(4-nitrophenyl)phosphate and paraoxon, and organophosphorus compound hydrolase inhibitors like 4-hydroxymercuribenzoate and EDTA confirmed that these esterases were of the carboxylesterase type.
journal_name
Biochem Pharmacoljournal_title
Biochemical pharmacologyauthors
Gaustad R,Berg T,Fonnum Fdoi
10.1016/0006-2952(92)90423-gsubject
Has Abstractpub_date
1992-08-18 00:00:00pages
827-9issue
4eissn
0006-2952issn
1873-2968pii
0006-2952(92)90423-Gjournal_volume
44pub_type
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journal_title:Biochemical pharmacology
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journal_title:Biochemical pharmacology
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
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journal_title:Biochemical pharmacology
pub_type: 杂志文章
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更新日期:1992-04-15 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1986-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2011-04-01 00:00:00