Importance of the cytochrome pathway of mitochondrial electron transport over the alternative pathway during the Kok effect in leaf discs of pea (Pisum sativum).

Abstract:

:The Kok effect refers to the progressive light-induced inhibition of dark respiration at low light intensities, which saturates around the light compensation point. This appears as a sudden break around the light compensation point in the plot of photosynthesis versus light intensity. The magnitude of the break can be considered as a measure of the Kok effect. In the present work, the importance of different components of dark respiration during the Kok effect was investigated by using low concentrations of mitochondrial inhibitors in leaf discs of pea (Pisum sativum L. cv. Azad P1). The effects of glucose (stimulates respiration) and 0.8 M sorbitol (imposes osmotic stress and inhibits photosynthesis) were also studied for comparison. The magnitude of the break decreased significantly in the presence of antimycin A or oligomycin (inhibitors of cytochrome pathway of mitochondrial electron transport and ATP synthase, respectively). In contrast, there was no significant change with salicylhydroxamic acid (SHAM; an inhibitor of alternative pathway of mitochondrial electron transport). The magnitude of the break increased significantly with glucose, and decreased on exposure to osmotic stress. Our results suggest that the Kok effect (inhibition of dark respiration in light) is modulated by inhibitors of cytochrome pathway and ATP synthesis, but not that of the alternative pathway.

journal_name

Physiol Plant

journal_title

Physiologia plantarum

authors

Padmavathi L,Raghavendra AS

doi

10.1034/j.1399-3054.2001.1130318.x

subject

Has Abstract

pub_date

2001-11-01 00:00:00

pages

430-434

issue

3

eissn

0031-9317

issn

1399-3054

pii

1130318

journal_volume

113

pub_type

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