Abstract:
:The modifying effect of sucrose on glutamate dehydrogenase (GDH) activity and isoenzyme pattern was investigated in isolated embryos of lupine (Lupinus luteus L.), cultured in vitro in a medium with sucrose (+S) or without sucrose (-S) and exposed to cadmium (Cd) and lead (Pb) stress. Sucrose starvation of lupine embryos led to a rapid increase in the specific activity of GDH, immunoreactive beta-polypeptide and it was accompanied by appearance of new cathodal isoforms of enzyme. This suggests that isoenzymes induced in lupine embryos by sucrose starvation combine into GDH hexamers with the predominance of beta-GDH subunits synthetized under GDH1 gene control. The addition of sucrose to the medium caused an opposite effect. Along with upregulation of catabolic activity of GDH by sucrose starvation, activity of proteolytic enzymes was also induced. These data can point to regulatory mechanism implying a sucrose dependent repression of the GDH1 gene according to the mechanism of catabolic repression. Treatment of embryos with Cd(2+) or Pb(2+) resulted in ammonium accumulation in the tissues, accompanied by an increase in anabolic activity of GDH and activity of anodal isoenzymes, in both (+S) and (-S) embryos without new de novo synthesis of alpha subunit proteins. Thus, GDH isoenzyme profiles may reflect the physiological function of GDH, which appears to be an important link of metabolic adaptation in cells, aimed at using carbon sources other than sugar during carbohydrate starvation (catabolic activity of GDH) and protecting plant tissues against ammonium accumulated because of heavy metal stress (anabolic activity of GDH).
journal_name
Physiol Plantjournal_title
Physiologia plantarumauthors
Lehmann T,Skrok A,Dabert Mdoi
10.1111/j.1399-3054.2009.01294.xsubject
Has Abstractpub_date
2010-01-01 00:00:00pages
35-47issue
1eissn
0031-9317issn
1399-3054pii
PPL1294journal_volume
138pub_type
杂志文章abstract::Dehydrins (DHNs; late embryogenesis abundant D-11) are a family of plant proteins induced in response to environmental stresses such as water stress, salinity and freezing or which occur during the late stages of embryogenesis. Previously, it was reported that citrus contains a small gene family encoding a unique clas...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/j.0031-9317.2004.0242.x
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journal_title:Physiologia plantarum
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doi:10.1111/j.1399-3054.2008.01157.x
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journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/ppl.12384
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journal_title:Physiologia plantarum
pub_type: 杂志文章
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journal_title:Physiologia plantarum
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doi:10.1111/ppl.13020
更新日期:2020-02-01 00:00:00
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journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/j.1399-3054.2009.01265.x
更新日期:2009-10-01 00:00:00
abstract::Uracil-DNA glycosylase (UDG) is the first enzyme in the base excision repair pathway for removal of uracil in DNA. DNA repair capacity is likely to be a critical factor in mutagenesis and thereby in the capacity to prevent genetic damage and unwanted variation. We have studied expression of UDG in 9 higher plant speci...
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pub_type: 杂志文章
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更新日期:1993-08-01 00:00:00
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journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/ppl.12915
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journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/j.1399-3054.2011.01545.x
更新日期:2012-02-01 00:00:00
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journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/j.1399-3054.2008.01131.x
更新日期:2008-10-01 00:00:00
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journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/j.1399-3054.2008.01122.x
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journal_title:Physiologia plantarum
pub_type: 杂志文章
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journal_title:Physiologia plantarum
pub_type: 杂志文章,评审
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更新日期:2011-05-01 00:00:00
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journal_title:Physiologia plantarum
pub_type: 杂志文章
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更新日期:2001-10-01 00:00:00
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journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:
更新日期:1996-07-01 00:00:00
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journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/ppl.13170
更新日期:2020-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1988-01-01 00:00:00
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journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/j.1399-3054.2008.01062.x
更新日期:2008-05-01 00:00:00
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journal_title:Physiologia plantarum
pub_type: 杂志文章
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更新日期:2014-07-01 00:00:00
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journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1034/j.1399-3054.2003.00049.x
更新日期:2003-03-01 00:00:00