Dynamics of Mhc evolution in birds and crocodilians: amplification of class II genes with degenerate primers.

Abstract:

:Genes of the major histocompatibility complex (Mhc) are the most polymorphic functional loci in mammalian populations, but little is known of Mhc variability in natural populations of nonmammalian vertebrates. To help extend such studies to birds and relatives, we present a pair of degenerate primers that amplify polymorphic segments of one chain (the beta chain) of the class II genes from the major histocompatibility complex (Mhc) of archosaurs (birds+crocodilians). The primers target two conserved regions lying within portions of the antigen-binding site (ABS) encoded by the second exon and amplify multiple genes from both genomic DNA and cDNA. The pattern of nucleotide substitution in ABS codons of 51 sequences amplified and cloned from five species of passerine birds and an alligator (Alligator mississippiensis) indicates that archosaurian class II beta genes are subject to selective forces similar to those operating in mammalian populations. Hybridization of a genomic clone generated by the primers revealed highly polymorphic bands in a sample of Florida scrub jays (Aphelocoma coerulescens coerulescens). Because the primers amplify only part of the ABS from multiple class II genes, they will be useful primarily for generating species specific clones, thereby providing a critical inroad to more detailed structural and evolutionary studies.

journal_name

Mol Ecol

journal_title

Molecular ecology

authors

Edwards SV,Grahn M,Potts WK

doi

10.1111/j.1365-294x.1995.tb00272.x

subject

Has Abstract

pub_date

1995-12-01 00:00:00

pages

719-29

issue

6

eissn

0962-1083

issn

1365-294X

journal_volume

4

pub_type

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