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Mouse brain opioid receptor identification by direct ultraviolet photoaffinity labeling.

Abstract：

:[3H]Morphine, PL-017[prolyl-3,4-3H,D-prolyl,3,4-3H] ([3H]PL-017) and enkephalin-(2-D-penicillamine,5-D-penicillamine)[tyrosyl-2,6-3H] ([3H]DPDPE) were directly cross-linked to mouse brain opiate receptors by an ultraviolet (254 nm) irradiation procedure. [3H]Morphine preferentially and specifically labeled a 58 kDa protein. The labeling of this protein was suppressed by the addition of excess naloxone. Dithiothreitol reduced the irreversible binding of [3H]morphine and [3H]PL-017 to the receptor protein. In the acid hydrolysate of [3H]DPDPE-labeled opiate receptors, dityrosine, was detected. These results suggest that the [3H]tyrosine residue of [3H]DPDPE covalently bound the tyrosine residue of delta-opioid receptors. The direct UV-photoaffinity labeling method using commercially available radioactive opiates described here should be a useful tool for characterization and purification of the opiate receptors.

journal_name

Biochem Pharmacol

journal_title

Biochemical pharmacology

authors

Nagamatsu K,Tagawa Y,Uchida S,Hasegawa A

doi

10.1016/0006-2952(93)90458-9

subject

Has Abstract

pub_date

1993-10-05 00:00:00

pages

1119-23

issue

7

eissn

0006-2952

issn

1873-2968

pii

0006-2952(93)90458-9

journal_volume

46

pub_type

杂志文章

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