P-glycoprotein expression on acute myeloid leukaemia blast cells at diagnosis predicts response to chemotherapy and survival.


:P-glycoprotein (Pgp) expression, which is associated with the multi-drug resistance (MDR) phenotype, has been reported to be a useful predictor of treatment outcome in acute leukaemia. We have examined the expression of Pgp on acute myeloid leukaemia (AML) cells in 54 newly diagnosed patients, using a novel streptavidin-biotin complex (ABC) technique. 55% of patients at diagnosis were positive for Pgp with JSB-1, a monoclonal antibody that binds to an internal epitope of Pgp. All patients received intensive induction chemotherapy. Post-remission treatment consisted of further chemotherapy +/- bone marrow transplantation. Complete remission (CR) rates were significantly lower in the Pgp positive group than in the Pgp negative group (60% v 92%; P = 0.02). The overall survival for Pgp-positive patients was significantly shorter (329 v 534d, P = 0.004), disease-free survival was also reduced but the difference was not statistically significant (median 277 v 522d, P = 0.16). In this study CD34 expression was not predictive of response to chemotherapy nor was it associated with Pgp expression. Our results confirm the prognostic value of Pgp expression in AML at diagnosis and we suggest that Pgp could be a useful therapeutic target for reversing multi-drug resistance. Furthermore, our simple and sensitive method of detecting Pgp should enable widespread testing to be performed.


Br J Haematol


Wood P,Burgess R,MacGregor A,Yin JA




Has Abstract


1994-07-01 00:00:00












  • Cultured human NK cells express the Ki-1/CD30 antigen.

    abstract::In this study we show that in vitro cultured human polyclonal NK cell lines and clones express the Ki-1/CD30 Hodgkin-associated antigen, identified by the BER-H2 monoclonal antibody. The percentage of BER-H2+ cells ranged from 19% to 67% in five polyclonal NK cell lines and was 31% and 20% in two NK clones. The intens...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Cambiaggi A,Cantoni C,Marciano S,De Totero D,Pileri S,Tazzari PL,Stein H,Ferrini S

    更新日期:1993-10-01 00:00:00

  • Evidence for a novel mechanism for gene amplification in multiple myeloma: 1q12 pericentromeric heterochromatin mediates breakage-fusion-bridge cycles of a 1q12 approximately 23 amplicon.

    abstract::Gene amplification is defined as a copy number (CN) increase in a restricted region of a chromosome arm, and is a mechanism for acquired drug resistance and oncogene activation. In multiple myeloma (MM), high CNs of genes in a 1q12 approximately 23 amplicon have been associated with disease progression and poor progno...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Sawyer JR,Tian E,Thomas E,Koller M,Stangeby C,Sammartino G,Goosen L,Swanson C,Binz RL,Barlogie B,Shaughnessy J

    更新日期:2009-11-01 00:00:00

  • Bone marrow cells from patients with Shwachman-Diamond syndrome abnormally express genes involved in ribosome biogenesis and RNA processing.

    abstract::Shwachman-Diamond Syndrome (SDS) is a multi-system genetic disorder with bone marrow failure. SBDS, the gene associated with SDS, has been postulated to play a role in ribosome biogenesis and RNA processing, but its functions are still unknown. To study whether these pathways are interrupted when Sbds protein is lost,...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Rujkijyanont P,Adams SL,Beyene J,Dror Y

    更新日期:2009-06-01 00:00:00

  • Effect of nitric oxide and nitric oxide donors on red blood cell oxygen transport.

    abstract::A mechanism has been proposed in which nitric oxide (NO) may bind to cysteine beta93 and be transported by haemoglobin from the lungs to the tissues and modify vascular tone. In addition, it has been reported that treatment of sickle cell anaemia blood with 80 p.p.m. NO gas in air shifts the oxygen affinity, as measur...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Hrinczenko BW,Alayash AI,Wink DA,Gladwin MT,Rodgers GP,Schechter AN

    更新日期:2000-08-01 00:00:00

  • Variable expression of CD3-zeta and associated protein tyrosine kinases in lymphocytes from patients with myeloid malignancies.

    abstract::In myeloid malignancies, T-cell and NK function has been shown to deteriorate with transformation from pre-leukaemia to advanced disease. Immune dysfunction in solid tumours has been attributed to abnormal signal transduction, possibly through altered expression of intracellular components of the TCR/CD3 complex (e.g....

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Buggins AG,Hirst WJ,Pagliuca A,Mufti GJ

    更新日期:1998-03-01 00:00:00

  • Maternal plasma or human serum albumin in wash buffer enhances enrichment and ex vivo expansion of human umbilical cord blood CD34+ cells.

    abstract::Umbilical cord blood is a valuable source of haemopoietic stem/progenitor cells (HSC) for transplantation. This study explored the effect of maternal plasma/human serum albumin (HSA) in the purification and culture conditions of CD34+ cells derived from human umbilical cord blood. During CD34+ cell enrichment, includi...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Kwok YK,Tang MH,Law HK,Ngai CS,Lau YL,Lau ET

    更新日期:2007-06-01 00:00:00

  • Characterization of a variant prothrombin in a patient congenitally deficient in factors II, VII, IX and X.

    abstract::An 18-month-old child, who had no evidence of liver disease, malabsorption, or chronic ingestion of coumarin compounds, was found to have plasma deficiencies of factors II, VII, IX and X. Assays for factor II and X by immunological techniques (antibody neutralization and immunoelectrophoresis) revealed normal or eleva...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Johnson CA,Chung KS,McGrath KM,Bean PE,Roberts HR

    更新日期:1980-03-01 00:00:00

  • Clofibric acid: a potential therapeutic agent in AML and MDS.

    abstract::Differentiation therapy using retinoic acids (RAs) or 1alpha25-dihydroxyvitamin D3 (D3) is an attractive alternative to chemotherapy in acute myeloid leukaemia (AML) and myelodysplastic syndromes (MDS). However, with the exception of RA therapy for acute promyelocytic leukaemia (APL), RAs and D3 are not potent enough ...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Fenton SL,Drayson MT,Hewison M,Vickers E,Brown G,Bunce CM

    更新日期:1999-05-01 00:00:00

  • The British Society for Haematology: 'What have the Romans ever done for us?'.

    abstract::'….Alright, but apart from the sanitation, medicine, education, wine, public order, irrigation, the roads, fresh water and public health………………what have the Romans ever done for us?' From Monty Python's Life of Brian An organizational review of the British Society for Haematology (BSH) was started in November 2013 and ...

    journal_title:British journal of haematology

    pub_type: 杂志文章,评审


    authors: Baglin T,Carrington P,Jackson G,Board of Trustees of the BSH.

    更新日期:2015-05-01 00:00:00

  • Kinetic analysis of 52Fe-labelled iron(III) hydroxide-sucrose complex following bolus administration using positron emission tomography.

    abstract::Kinetic analysis of a single intravenous injection of 100 mg iron(III) hydroxide-sucrose complex (Venofer) mixed with 52Fe(III) hydroxide-sucrose as a tracer was followed for 3-6 h in four generally anaesthetized, artificially ventilated minipigs using positron emission tomography (PET). The amount of injected radioac...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Beshara S,Lundqvist H,Sundin J,Lubberink M,Tolmachev V,Valind S,Antoni G,Långström B,Danielson BG

    更新日期:1999-02-01 00:00:00

  • Circulating immune complexes involving the ABO system after platelet transfusion.

    abstract::It has been proposed that when ABO unmatched platelets are transfused circulating immune complexes (CIC) may be formed between the patient's soluble ABH antigens and the transfused antibodies. Platelets might then be destroyed by bystander mechanisms or by the binding of CIC to the Fc receptor or to C3 binding membran...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Heal JM,Masel D,Rowe JM,Blumberg N

    更新日期:1993-11-01 00:00:00

  • Co-administration of Flt-3 ligand counteracts the actions of thrombopoietin in myelosuppressed rhesus monkeys.

    abstract::This placebo-controlled study evaluated the efficacy of Flt-3 ligand (FL) combined with TPO in myelosuppressed rhesus monkeys. The monkeys were subjected to 5 Gy total body irradiation (TBI), resulting in 3 weeks of profound pancytopenia, and received either 5 microg/kg of rhesus TPO i.v. on d 1 (n = 4) and 100 microg...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Hartong SC,Neelis KJ,Wagemaker G

    更新日期:2003-04-01 00:00:00

  • Myeloma-reactive allospecific cytotoxic T lymphocytes lyse target cells via the granule exocytosis pathway.

    abstract::Accumulating evidence indicates that a graft-vs.-myeloma effect (GVM) and its associated clinical remission of the disease can be induced by donor lymphocyte infusion in myeloma patients who have relapsed after allogeneic bone marrow transplantation. Although it is believed that GVM is induced by allospecific T cells,...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Chiriva-Internati M,Du J,Cannon M,Barlogie B,Yi Q

    更新日期:2001-02-01 00:00:00

  • How to manage invasive procedures in children with haemophilia.

    abstract::Invasive procedures can be performed safely in children with haemophilia due to the availability of factor VIII/IX for patients without inhibitors. Most guidelines are based on the experiences in adults, but still there is no established consensus on the optimal factor levels or duration of replacement therapy for adu...

    journal_title:British journal of haematology

    pub_type: 杂志文章,评审


    authors: Ljung RC,Knobe K

    更新日期:2012-06-01 00:00:00

  • A single high dose of idarubicin combined with high-dose ARA-C for treatment of first relapse in childhood 'high-risk' acute lymphoblastic leukaemia: a study of the AIEOP group.

    abstract::The outcome of children with acute lymphoblastic leukaemia (ALL) and early relapse remains unsatisfactory. In January 1995, the AIEOP (Associazione Italiana di Oncologia ed Ematologia Pediatrica) group opened a trial for children with ALL in first isolated or combined bone marrow relapse defined at high risk according...

    journal_title:British journal of haematology

    pub_type: 临床试验,杂志文章,多中心研究


    authors: Testi AM,Del Giudice I,Arcese W,Moleti ML,Giona F,Basso G,Biondi A,Conter V,Messina C,Rondelli R,Micozzi A,Micalizzi C,Barisone E,Locatelli F,Dini G,Aricò M,Casale F,Comis M,Ladogana S,Lippi A,Mura R,Pinta MF,

    更新日期:2002-09-01 00:00:00

  • Plasma clearance of transferrin in control and hypotransferrinaemic mice: implications for regulation of transferrin turnover.

    abstract::Kinetic studies were performed to determine the clearance of iodinated transferrin in hypotransferrinaemic mice, as compared to normal animals. Clearance of i.v. (and i.p.) administered radiolabelled protein in homozygous (hpx/hpx) mice was significantly faster than in heterozygous (hpx/+) and wild-type control (+/+) ...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Raja KB,Simpson RJ,Peters TJ

    更新日期:1995-01-01 00:00:00

  • Allogeneic haematopoietic cell transplantation for myelofibrosis in 30 patients 60-78 years of age.

    abstract::We analysed the results of haematopoietic cell transplantation (HCT) in 30 patients aged 60-78 (median 65) years, with primary myelofibrosis or myelofibrosis evolving from antecedent polycythaemia vera or essential thrombocythaemia. Donors were human leucocyte antigen (HLA)-identical siblings (N = 15) or unrelated ind...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Samuelson S,Sandmaier BM,Heslop HE,Popat U,Carrum G,Champlin RE,Storb R,Prchal JT,Gooley TA,Deeg HJ

    更新日期:2011-04-01 00:00:00

  • Haemoglobin F levels in sudden infant deaths.

    abstract::Fetal haemoglobin levels have been measured prospectively in 135 autopsy cases of sudden, unexpected infant deaths (31 pre-term, 104 full term) using standard laboratory methods. These results have been compared with Hb F values from a normal control group of 570 living infants (145 pre-term, 425 full-term) with a pos...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Fagan DG,Walker A

    更新日期:1992-10-01 00:00:00

  • Primary thrombocythaemia associated with systemic mastocytosis: a report of five cases.

    abstract::Mastocytosis is an uncommon disease characterized by a proliferation of tissue mast cells involving various organs, particularly the bone marrow. Though rare, the association of mastocytosis and haematological malignancies is well established. However, the frequency of mastocytosis reported in patients with essential ...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Le Tourneau A,Gaulard P,D'Agay MF,Vainchencker W,Cadiou M,Devidas A,Haioun C,Clauvel JP,Audouin J,Diebold J

    更新日期:1991-09-01 00:00:00

  • Analysis of heat-shock protein expression in myeloid leukaemia cells by flow cytometry.

    abstract::Expression of heat-shock proteins (hsp) was analysed in the leukaemic cells of 12 patients with acute myeloid leukaemia (AML) and nine patients with chronic myeloid leukaemia (CML). Using monoclonal antibodies to hsp70, hsp90 and hsp60 (ML30, a mycobacterial antigen with homology to human hsp60), we measured hsp level...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Chant ID,Rose PE,Morris AG

    更新日期:1995-05-01 00:00:00

  • Normal glutathione content and some related enzyme activities in the fetal erythrocytes.

    abstract::Pure fetal blood was obtained by direct-vision fetoscopy from 66 fetuses at 17-24 weeks gestation. The concentration of GSH and the activities of the enzymes gamma-glutamylcysteine synthetase (GCS), glutathione synthetase (GS), glutathione reductase (GR) and glutathione peroxidase (GPx) were analysed by established te...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Lestas AN,Rodeck CH

    更新日期:1984-08-01 00:00:00

  • Role of Epstein-Barr virus and soluble CD21 in persistent polyclonal B-cell lymphocytosis.

    abstract::The expression of EBV proteins and immunological properties were studied in the first stable cell line (SM) established from a patient presenting with persistent polyclonal B-cell lymphocytosis (PPBL). SM cells which represent a small population of EBV-positive atypical cells found in the peripheral blood of the patie...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Larcher C,Fend F,Mitterer M,Prang N,Schwarzmann F,Huemer HP

    更新日期:1995-07-01 00:00:00

  • A single-centre prospective study of clinical and haemostatic risk factors for venous thromboembolism following lower limb arthroplasty.

    abstract::Previous studies report conflicting results concerning the potential significance of thrombophilic genotypes in postarthroplasty venous thromboembolism (VTE). This study assessed thrombophilic genotypes, haemostatic and clinical variables as independent risk factors for VTE postarthroplasty. A total number of 569 pati...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Joseph JE,Low J,Courtenay B,Neil MJ,McGrath M,Ma D

    更新日期:2005-04-01 00:00:00

  • Actin pools and actin microfilament organization in cultured human endothelial cells after exposure to thrombin.

    abstract::Human umbilical vein endothelial cells (HUVEC) in primary confluent cultures lost their normal polygonal shape and assumed a 'contracted' appearance as judged by phase contrast microscopy when exposed to highly purified bovine thrombin (2 N.I.H. u/ml). Total actin in thrombin-exposed cells did not differ from that of ...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Galdal KS,Evensen SA,Høglund AS,Nilsen E

    更新日期:1984-12-01 00:00:00

  • Evaluation of a new UVB source for irradiation of platelet concentrates.

    abstract::The application of ultraviolet B (UVB) radiation has been proposed as a new technology to decrease immunogenicity of leucocytes in platelet transfusions. UV radiation also induces platelet aggregation, which occurs most effectively at wavelengths between 240 and 280 nm and falls off sharply above 300 nm. In order to m...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: van Prooijen HC,van Marwijk Kooy M,van Weelden H,Aarts-Riemens MI,Borghuis L,Akkerman JW

    更新日期:1990-08-01 00:00:00

  • A comparison of molecular and enzyme-based assays for the detection of thiopurine methyltransferase mutations.

    abstract::S-Methylation by thiopurine methyltransferase (TPMT) is an important route of metabolism for the thiopurine drugs. About one in 300 individuals are homozygous for a TPMT mutation associated with very low enzyme activity and severe myelosuppression if treated with standard doses of drug. To validate the use of molecula...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Coulthard SA,Rabello C,Robson J,Howell C,Minto L,Middleton PG,Gandhi MK,Jackson G,McLelland J,O'Brien H,Smith S,Reid MM,Pearson AD,Hall AG

    更新日期:2000-09-01 00:00:00

  • Quiescent CD34+ early erythroid progenitors are resistant to several erythropoietic 'inhibitory' cytokines; role of FLIP.

    abstract::In this study, quiescent bone marrow-derived CD34+ erythroid burst-forming units (BFU-E) were found to be resistant to the inhibitory effects of tumour necrosis factor (TNF)-alpha and -beta as well as interferon (IFN)-alpha, -beta and -gamma, in contrast to those stimulated by a combination of erytrhropoietin (Epo) pl...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Ratajczak J,Kucia M,Reca R,Zhang J,Machalinski B,Ratajczak MZ

    更新日期:2003-10-01 00:00:00

  • Splanchnic vein thrombosis in myeloproliferative neoplasms.

    abstract::Splanchnic vein thrombosis (SVT) is one of the most important complications of myeloproliferative neoplasms (MPN). Although MPN are common causes of SVT, the pathophysiological mechanisms underlying this predisposition, their epidemiology and natural history are not fully understood. Studies have concentrated on the g...

    journal_title:British journal of haematology

    pub_type: 杂志文章,评审


    authors: Sekhar M,McVinnie K,Burroughs AK

    更新日期:2013-09-01 00:00:00

  • Heterogeneity of alleles encoding high- and low-prevalence red blood cell antigens across Africa: useful data to facilitate transfusion in African patients.

    abstract::Ethnic variations in red blood cell (RBC) antigens can be a source of alloimmunization, especially in migrant populations. To improve transfusion safety in continental Africa and countries with African migrants, we performed RBC genotyping to determine allele frequencies coding for high- and low-prevalence antigens. A...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Silvy M,Beley S,Granier T,Ba A,Chiaroni J,Bailly P

    更新日期:2013-11-01 00:00:00

  • Primary plasma cell leukaemia.

    abstract::Among 750 previously untreated patients with multiple myeloma, 27 (4%) presented with plasma cell leukaemia. All but one patient had high tumour mass and, when compared with comparable patients without leukaemia, more frequent extraosseous involvement, thrombocytopenia, high serum lactate dehydrogenase and hypodiploid...

    journal_title:British journal of haematology

    pub_type: 临床试验,杂志文章


    authors: Dimopoulos MA,Palumbo A,Delasalle KB,Alexanian R

    更新日期:1994-12-01 00:00:00